Ptprt (NM_001291149) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	Myc-DDK
Synonyms	mKIAA0283; mRPTPrho; R-PTP-T; RPTP-rfo; RPTP-rho; RPTPmam4; RPTPrho
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MR231806 representing NM_001291149 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGGAGCCTTGGCGGGCTCGCCCTCTGCCTGCTCCGGCTCCTGCTCCTGGGGCTGCAGCGGCCGCCGT TGCCCGGCGCCGGAGCGCAGAGCGCCGCAGGTGGCTGTTCTTTTGACGAACATTACAGCAACTGCGGGTA TAGCGTGGCTCTGGGAACCAATGGGTTTACCTGGGAGCAGATTAACACATGGGAGAAGCCAATGCTGGAC CCAGCTGTGCCCACAGGGTCCTTCATGATGGTGAACAGCTCTGGAAGGGCTTCAGGCCAGAAAGCCCATC TTCTTCTGCCAACCTTGAAGGAGAATGACACTCACTGCATTGACTTCCATTACTACTTCTCCAGTCGAGA TCGCTCCAGCCCGGGAGCCTTGAATGTCTACGTGAAGGTGAATGGTGGACCCCAAGGGAACCCTGTCTGG AACGTATCTGGCGTCGTCACTGAGGGCTGGGTGAAGGCAGAGCTTGCCATCAGCACCTTCTGGCCTCATT TCTATCAGGTGATATTTGAATCCGTCTCTTTGAAAGGTCATCCTGGTTACATCGCTGTGGACGAAGTTCG GGTCCTTGCTCATCCATGCAGAAAAGCACCTCATTTCCTGCGACTCCAAAACGTTGAGGTGAATGTGGGG CAGAACGCCACGTTTCAGTGCATTGCTGGTGGAAAGTGGTCCCAGCATGACAAACTTTGGCTCCAGCAAT GGAATGGCAGAGACACAGCCCTCATGGTCACCCGGGTGGTCAACCACAGACGCTTCTCAGCCACAGTGAG TGTGGCAGACACCTCTCAACGCAGTATCTCCAAGTATCGCTGCGTGATCCGCTCAGATGGTGGGTCTGGT GTGTCCAACTATGCAGAGCTGATTGTGAAAGAGCCTCCCACGCCCATTGCTCCCCCGGAACTACTGGCCG TGGGTGCCACCTACCTGTGGATTAAACCAAATGCCAACTCCATTATTGGGGATGGCCCCATCATACTGAA GGAGGTAGAATACCGCACAACCACAGGAACCTGGGCCGAGACCCACATCGTGGACTCTCCCAACTACAAG CTCTGGCATCTGGACCCTGATGTGGAGTATGAGATCCGGGTGCTGCTCACACGACCAGGAGAGGGGGGCA CAGGACCACCAGGACCACCCCTAACTACCAGGACCAAGTGTGCCGATCCCGTGCATGGCCCGCAGAATGT GGAGATTGTGGACATTCGGGCTCGGCAGCTGACCCTGCAGTGGGAACCCTTTGGCTATGCAGTGACCCGC TGCCACAGCTACAACCTCACAGTGCAGTACCAGTACGTGTTCAACCAGCAGCAGTATGAAGCTGAAGAGG TGATCCAGACATCTTCCCACTATACCCTTCGGGGTCTACGGCCCTTCATGACCATCAGACTGCGGCTGCT ACTGTCCAACCCTGAGGGCCGGATGGAGAGTGAGGAGTTGGTGGTACAGACAGAAGAGGATGTTCCAGGA GCTGTTCCTCTCGAGTCCATCCAAGGGGGTCCCTTTGAGGAGAAGATCTACATCCAGTGGAAACCTCCCA ATGAGACCAATGGGGTCATCACACTCTATGAGATTAACTACAAGGCTGTGGGCTCACTGGATCCAAGTGC TGACCTCTCCAGCCAGAGGGGAAAGGTGTTCAAACTCCGGAATGAAACCCACCACCTCTTTGTGGGTCTG TACCCTGGGACTACCTACTCTTTCACCATCAAGGCCAGCACAGCCAAGGGCTTTGGACCCCCAGTCACTA CTCGGATTGCCACCAAGATTTCAGCTCCATCAATGCCTGAATATGACGCAGATACCCCACTCAATGAAAC AGATACAACCATCACGGTGATGCTCAAACCCGCCCAGTCCAGGGGAGCCCCAGTCAGTGTTTACCAGCTG GTTGTCAAGGAGGAGCGACTCCAGAAGTCTCGGAGAGCAGCTGACATCATTGAGTGTTTCTCAGTACCTG TGAGCTACCGGAACGCCTCCAACCTCGATTCGCTACACTACTTTGCTGCTGAGCTGAAGCCCTCCAACCT GCCTGTCACCCAGCCATTTACAGTGGGAGACAACAAGACCTACAATGGCTACTGGAACCCTCCTCTCTCC CCATTGAAGAGCTACAGCATCTACTTCCAGGCCCTCAGCAAAGCAAATGGAGAGACAAAAATCAACTGTG TGCGTCTGGCTACAAAAGGTGCTTCCACTCAGAATTCTAACACTGTGGAGCCGGAGAAGCAGGTCGACAA CACGGTGAAGATGGCTGGCGTGATTGCTGGCCTCCTCATGTTCATCATCATTCTCCTGGGGGTGATGCTG ACCATCAAAAGGAGGAAGCTGGCTAAGAAGCAGAAGGAGACCCAGAGTGGAGCCCAGAGGGAGATGGGTC CTGTGGCCTCGACTGACAAGCCTACCGCCAAGCTCGGCACCAACCGCAATGATGAAGGCTTCTCCTCCAG CTCTCAGGATGTTAATGGATTCACAGATGGCAGCCGTGGGGAGCTGTCTCAGCCTACCCTCACCATTCAG ACTCATCCCTACCGGACTTGTGACCCTGTAGAGATGAGCTATCCCCGGGACCAGTTCCAGCCTGCCATCC GGGTGGCGGACCTGCTTCAACACATCACCCAGATGAAGAGAGGCCAGGGCTACGGGTTCAAGGAGGAATA CGAGGCCTTACCAGAAGGACAGACAGCTTCGTGGGACACAGCCAAGGAAGATGAAAACCGCAATAAGAAT CGATACGGAAACATCATATCTTATGACCACTCTCGAGTAAGGCTGTTGGTGCTGGATGGAGACCCTCACT CAGACTACATCAATGCCAACTACATTGACGGGTACCACCGACCCCGGCACTACATTGCAACCCAAGGTCC AATGCAAGAGACGGTGAAGGACTTTTGGAGAATGATCTGGCAGGAAAACTCTGCCAGCATCGTCATGGTC ACAAACCTTGTGGAAGTGGGCAGGCACCCAGCGGAACACACTGTGGGAACTGCCACGCTAGGCCGTGCTG CATCCCCCGGAATGGTGAAGTGTGTCCGATACTGGCCAGATGACACAGAGGTCTATGGAGACATTAAAGT CACCCTAATAGAAACAGAGCCCCTGGCAGAATACGTCATCCGCACCTTCACAGTCCAGAAGAAAGGCTAC CATGAGATCCGGGAGCTCCGCCTCTTCCACTTCACCAGCTGGCCTGACCACGGTGTTCCCTGCTATGCCA CTGGCCTTCTGGGCTTTGTCCGCCAGGTCAAGTTTCTCAATCCCCCAGAAGCTGGGCCCATAGTGGTCCA TTGCAGTGCTGGAGCCGGGAGGACTGGCTGCTTCATTGCGATTGACACCATGCTCGACATGGCTGAGAAT GAAGGGGTTGTGGACATCTTCAACTGTGTGCGTGAGCTCCGGGCACAGAGGGTCAACCTGGTGCAGACAG AGGAGCAGTACGTGTTTGTGCACGATGCCATCCTGGAAGCATGCCTCTGCGGCAATACTGCCATCCCAGT GTGTGAGTTCCGCTCTCTCTACTACAACATCAGCAGGCTGGACCCGCAGACCAACTCCAGCCAGATCAAA GACGAGTTTCAGACACTCAACATTGTGACACCTCGAGTGCGGCCTGAAGACTGCAGCATTGGGCTCTTAC CCCGGAACCATGATAAGAATCGGAGCATGGATGTCCTGCCTCTGGACCGCTGTCTACCCTTCCTCATCTC AGTAGATGGAGAGTCCAGCAACTACATCAATGCAGCACTGATGGATAGCCACAAGCAGCCTGCCGCCTTC GTGGTCACCCAGCATCCTCTACCCAACACGGTGGCAGACTTCTGGAGGCTGGTGTTTGATTATAATTGTT CATCTGTGGTGATGCTGAACGAGATGGATACTGCTCAGCTCTGTATGCAGTACTGGCCTGAGAAGACCTC CGGGTGTTATGGTCCCATCCAGGTGGAGTTTGTCTCTGCAGACATCGATGAGGACATCATCCACAGAATC TTCCGGATCTGTAACATGGCTCGGCCACAGGATGGTTATCGTATTGTCCAGCACCTCCAGTACATCGGCT GGCCTGCATACCGGGACACGCCCCCCTCTAAGCGCTCTCTGCTCAAAGTGGTCCGACGGCTGGAGAAATG GCAGGAGCAATACGACGGAAGAGAGGGGCGCACTGTGGTCCACTGCCTAAATGGGGGAGGCCGCAGTGGA ACCTTCTGTGCTATCTGCAGTGTGTGTGAGATGATCCAGCAGCAGAACATTATTGACGTGTTCCACATTG TGAAAACCCTCCGCAACAACAAGTCCAACATGGTGGAGACGCTGGAACAGTATAAATTTGTATATGAGGT GGCACTGGAATATTTAAGCTCCTTT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >MR231806 representing NM_001291149 Red=Cloning site Green=Tags(s) MGSLGGLALCLLRLLLLGLQRPPLPGAGAQSAAGGCSFDEHYSNCGYSVALGTNGFTWEQINTWEKPMLD PAVPTGSFMMVNSSGRASGQKAHLLLPTLKENDTHCIDFHYYFSSRDRSSPGALNVYVKVNGGPQGNPVW NVSGVVTEGWVKAELAISTFWPHFYQVIFESVSLKGHPGYIAVDEVRVLAHPCRKAPHFLRLQNVEVNVG QNATFQCIAGGKWSQHDKLWLQQWNGRDTALMVTRVVNHRRFSATVSVADTSQRSISKYRCVIRSDGGSG VSNYAELIVKEPPTPIAPPELLAVGATYLWIKPNANSIIGDGPIILKEVEYRTTTGTWAETHIVDSPNYK LWHLDPDVEYEIRVLLTRPGEGGTGPPGPPLTTRTKCADPVHGPQNVEIVDIRARQLTLQWEPFGYAVTR CHSYNLTVQYQYVFNQQQYEAEEVIQTSSHYTLRGLRPFMTIRLRLLLSNPEGRMESEELVVQTEEDVPG AVPLESIQGGPFEEKIYIQWKPPNETNGVITLYEINYKAVGSLDPSADLSSQRGKVFKLRNETHHLFVGL YPGTTYSFTIKASTAKGFGPPVTTRIATKISAPSMPEYDADTPLNETDTTITVMLKPAQSRGAPVSVYQL VVKEERLQKSRRAADIIECFSVPVSYRNASNLDSLHYFAAELKPSNLPVTQPFTVGDNKTYNGYWNPPLS PLKSYSIYFQALSKANGETKINCVRLATKGASTQNSNTVEPEKQVDNTVKMAGVIAGLLMFIIILLGVML TIKRRKLAKKQKETQSGAQREMGPVASTDKPTAKLGTNRNDEGFSSSSQDVNGFTDGSRGELSQPTLTIQ THPYRTCDPVEMSYPRDQFQPAIRVADLLQHITQMKRGQGYGFKEEYEALPEGQTASWDTAKEDENRNKN RYGNIISYDHSRVRLLVLDGDPHSDYINANYIDGYHRPRHYIATQGPMQETVKDFWRMIWQENSASIVMV TNLVEVGRHPAEHTVGTATLGRAASPGMVKCVRYWPDDTEVYGDIKVTLIETEPLAEYVIRTFTVQKKGY HEIRELRLFHFTSWPDHGVPCYATGLLGFVRQVKFLNPPEAGPIVVHCSAGAGRTGCFIAIDTMLDMAEN EGVVDIFNCVRELRAQRVNLVQTEEQYVFVHDAILEACLCGNTAIPVCEFRSLYYNISRLDPQTNSSQIK DEFQTLNIVTPRVRPEDCSIGLLPRNHDKNRSMDVLPLDRCLPFLISVDGESSNYINAALMDSHKQPAAF VVTQHPLPNTVADFWRLVFDYNCSSVVMLNEMDTAQLCMQYWPEKTSGCYGPIQVEFVSADIDEDIIHRI FRICNMARPQDGYRIVQHLQYIGWPAYRDTPPSKRSLLKVVRRLEKWQEQYDGREGRTVVHCLNGGGRSG TFCAICSVCEMIQQQNIIDVFHIVKTLRNNKSNMVETLEQYKFVYEVALEYLSSF TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001291149
ORF Size	4365 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001291149.1, NP_001278078.1
RefSeq Size	12159 bp
RefSeq ORF	4368 bp
Locus ID	19281
UniProt ID	Q99M80
MW	163.6 kDa
Gene Summary	May be involved in both signal transduction and cellular adhesion in the CNS. May have specific signaling roles in the tyrosine phosphorylation/dephosphorylation pathway in the anterior compartment of the adult cerebellar cortex.[UniProtKB/Swiss-Prot Function]

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