Dnmt1 (NM_001199432) Mouse Tagged ORF Clone
CAT#: MR231820
- TrueORF®
Dnmt1 (myc-DDK-tagged) - Mouse DNA methyltransferase (cytosine-5) 1 (Dnmt1), transcript variant 3
"NM_001199432" in other vectors (1)
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CNY 15,010.00
CNY 300.00
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | Cxxc9; Dnmt; Dnmt1o; m.MmuI; MCMT; Met-1; Met1; MommeD; MommeD2; MTa; MTase |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MR231820 representing NM_001199432
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCAGACTCAAATAGATCCCCAAGATCCAGGCCCAAGCCTCGGGGACCCAGGAGAAGCAAGTCGGACA GTGACACCCTTTTTGAAACTTCACCTAGTTCCGTGGCTACGAGGAGAACCACCAGGCAGACCACCATCAC GGCTCACTTCACGAAGGGCCCCACTAAACGGAAACCCAAGGAAGAGTCGGAAGAGGGGAACTCGGCTGAG TCGGCTGCAGAGGAGAGAGACCAGGATAAGAAACGCAGAGTTGTAGACACAGAGAGTGGTGCTGCAGCTG CTGTGGAGAAACTGGAAGAGGTAACAGCGGGAACCCAGCTGGGTCCGGAAGAGCCATGTGAACAGGAAGA TGACAACAGGAGTCTTCGACGTCACACCAGAGAGCTATCATTGAGGCGGAAATCAAAGGAGGATCCAGAC AGAGAAGCAAGACCGGAAACTCACTTGGACGAGGACGAGGACGGAAAAAAGGATAAAAGAAGTTCCAGAC CCAGGAGCCAGCCCAGAGATCCAGCTGCCAAACGGAGACCCAAGGAAGCAGAGCCAGAGCAGGTAGCTCC AGAGACTCCCGAGGACAGAGACGAGGATGAGAGGGAGGAGAAGAGACGAAAAACGACACGTAAAAAACTG GAGTCACACACCGTTCCCGTTCAGAGCAGATCGGAGAGAAAAGCCGCTCAAAGCAAAAGTGTGATCCCGA AGATCAACTCACCAAAGTGCCCCGAGTGTGGCCAGCACCTAGACGACCCTAACCTGAAGTACCAGCAGCA CCCTGAGGATGCTGTGGATGAACCCCAGATGTTGACCAGTGAGAAACTGTCCATCTACGACTCCACCTCG ACCTGGTTTGATACTTATGAAGATTCTCCCATGCATAGGTTCACTTCCTTCAGTGTGTACTGCAGTCGCG GGCACCTGTGTCCTGTCGACACCGGTCTCATTGAGAAGAATGTAGAGCTCTACTTTTCTGGGTGTGCCAA AGCAATTCATGACGAGAATCCATCTATGGAAGGTGGTATTAATGGCAAAAACCTCGGGCCAATCAATCAG TGGTGGCTCAGTGGCTTTGATGGTGGCGAGAAGGTGCTCATTGGCTTCTCCACTGCATTTGCTGAATACA TTTTGATGGAGCCCAGCAAAGAGTATGAGCCAATATTTGGGCTGATGCAGGAGAAAATTTACATCAGCAA GATTGTTGTTGAGTTCCTGCAAAACAATCCTGATGCTGTATATGAAGACCTGATCAATAAGATTGAGACC ACTGTTCCTCCTTCTACCATTAATGTGAACCGGTTCACAGAGGACTCCCTCTTACGCCACGCCCAGTTTG TAGTGAGCCAGGTAGAGAGTTACGACGAAGCCAAGGACGATGATGAGACCCCCATCTTCTTGTCTCCCTG TATGAGAGCCCTGATCCATTTGGCTGGTGTCTCCCTGGGACAGAGGCGAGCAACAAGGCGCGTCATGGGT GCTACCAAGGAGAAGGACAAAGCACCCACGAAAGCCACCACCACCAAGCTGGTCTATCAGATCTTTGACA CTTTCTTCTCAGAGCAGATTGAGAAGTATGATAAGGAGGACAAGGAGAATGCCATGAAGCGCCGCCGCTG TGGTGTCTGTGAGGTCTGTCAGCAGCCTGAGTGTGGGAAGTGCAAGGCGTGCAAAGATATGGTGAAGTTT GGTGGCACTGGACGGAGTAAGCAGGCTTGCCTCAAGAGGAGGTGTCCTAACTTGGCGGTGAAGGAGGCAG ACGACGATGAAGAGGCTGATGATGATGTGTCAGAGATGCCATCACCCAAAAAGCTGCATCAGGGGAAGAA GAAGAAGCAGAACAAGGACCGCATCTCCTGGCTTGGGCAGCCTATGAAGATTGAAGAGAATAGAACTTAC TATCAGAAGGTGAGCATCGATGAGGAGATGCTAGAGGTGGGCGACTGCGTCTCGGTCATTCCAGATGATT CCTCCAAACCACTCTATCTAGCCAGGGTCACAGCTCTGTGGGAAGACAAAAATGGTCAGATGATGTTCCA TGCGCACTGGTTCTGCGCTGGGACAGACACAGTCCTGGGAGCCACCTCCGACCCCCTGGAACTGTTCCTG GTGGGCGAGTGCGAAAACATGCAGCTTTCCTACATCCACAGCAAGGTCAAGGTCATCTACAAAGCCCCTT CTGAAAACTGGGCCATGGAGGGAGGCACAGACCCTGAGACCACACTGCCTGGGGCTGAGGATGGCAAGAC TTACTTCTTCCAGCTCTGGTACAACCAGGAGTACGCAAGGTTTGAATCCCCACCCAAGACCCAGCCGACC GAGGACAACAAGCACAAGTTCTGCCTATCTTGTATCCGGCTGGCTGAGCTGAGACAAAAAGAAATGCCCA AGGTCCTGGAACAAATTGAGGAGGTGGATGGCCGGGTCTACTGCAGTTCCATCACCAAGAATGGTGTTGT CTACCGACTGGGTGACAGTGTGTACCTTCCTCCCGAGGCCTTTACTTTCAACATCAAAGTGGCTAGCCCC GTGAAACGCCCAAAGAAGGATCCTGTGAACGAGACCCTGTACCCTGAGCACTACCGCAAGTATTCTGACT ACATCAAGGGGAGCAACCTGGATGCTCCAGAGCCCTATCGCATCGGTCGGATAAAAGAGATCCACTGTGG CAAGAAGAAAGGCAAGGTCAACGAGGCAGACATCAAGCTGAGGCTCTACAAGTTCTACAGGCCTGAGAAT ACCCACAGGTCCTACAACGGATCCTATCACACTGACATCAACATGCTTTACTGGAGCGACGAGGAAGCTG TGGTGAACTTCAGCGACGTGCAGGGCCGCTGTACCGTGGAGTACGGGGAAGACCTACTTGAGAGCATCCA GGATTATTCACAAGGGGGCCCTGACCGCTTCTACTTCCTCGAGGCCTACAATTCAAAGACCAAGAACTTT GAAGACCCACCAAACCATGCCCGCAGCCCTGGGAACAAAGGGAAAGGGAAGGGGAAAGGGAAGGGGAAGG GGAAGCATCAGGTGTCAGAGCCCAAAGAGCCTGAGGCAGCCATCAAACTGCCCAAGCTCCGGACCCTGGA TGTGTTTTCCGGCTGTGGAGGGTTATCGGAAGGATTCCACCAAGCAGGCATCTCGGAAACGCTGTGGGCC ATCGAGATGTGGGACCCGGCAGCCCAGGCATTTCGGCTGAACAACCCCGGCACCACAGTGTTCACAGAGG ACTGCAACGTGCTTCTTAAGCTGGTCATGGCTGGGGAGGTGACCAACTCTCTGGGCCAAAGGCTGCCACA GAAGGGCGATGTGGAGATGCTGTGTGGTGGGCCACCCTGCCAGGGCTTCAGTGGCATGAACCGCTTCAAC TCCCGCACTTACTCCAAGTTCAAAAACTCCCTAGTGGTCTCCTTCCTCAGCTACTGTGACTACTACCGGC CTCGGTTCTTCCTTCTGGAGAACGTCAGGAACTTCGTGTCCTACAGACGCTCCATGGTGCTGAAGCTCAC ACTGCGCTGCCTGGTCCGCATGGGCTACCAGTGCACCTTTGGTGTGCTCCAGGCTGGACAGTATGGCGTG GCCCAGACACGAAGGAGGGCCATCATCTTGGCTGCAGCCCCAGGAGAAAAGCTGCCTCTGTTCCCAGAGC CTCTGCATGTGTTTGCGCCCCGTGCCTGCCAGCTGAGCGTTGTGGTGGATGACAAGAAGTTTGTTAGCAA CATAACGAGGCTGAGCTCGGGGCCCTTCCGAACCATCACCGTGCGAGACACCATGTCTGACCTCCCCGAG ATCCAGAATGGAGCCTCGAATTCTGAGATCCCCTACAATGGAGAGCCACTGTCCTGGTTCCAGAGGCAGC TGCGAGGATCACACTACCAGCCCATCCTCAGGGACCATATCTGCAAGGACATGAGCCCACTGGTGGCTGC CCGCATGCGGCACATCCCACTGTTCCCAGGATCAGATTGGCGTGACCTGCCCAACATACAGGTGCGGCTG GGAGATGGCGTCATAGCCCATAAGCTACAGTACACCTTTCATGATGTGAAAAATGGCTACAGCAGCACCG GTGCCCTGCGTGGAGTCTGTTCCTGTGCAGAAGGCAAGGCCTGCGACCCTGAGTCCAGGCAATTCAGCAC CCTCATCCCCTGGTGCCTGCCGCACACTGGGAACCGGCACAACCACTGGGCTGGCCTCTACGGGCGTCTG GAGTGGGATGGCTTCTTCAGCACCACTGTCACCAACCCTGAGCCCATGGGCAAGCAGGGTCGGGTGCTCC ACCCGGAGCAGCACCGGGTCGTGAGTGTTCGGGAATGTGCCCGCTCCCAGGGCTTTCCAGATAGCTACCG GTTCTTCGGCAACATCCTGGACAGACACCGGCAGGTGGGTAATGCTGTGCCACCACCCCTGGCCAAAGCC ATTGGCCTGGAGATTAAGCTCTGCCTGCTGTCCAGTGCTCGGGAGAGCGCATCAGCTGCAGTTAAAGCAA AAGAGGAGGCTGCTACCAAGGAC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR231820 representing NM_001199432
Red=Cloning site Green=Tags(s) MADSNRSPRSRPKPRGPRRSKSDSDTLFETSPSSVATRRTTRQTTITAHFTKGPTKRKPKEESEEGNSAE SAAEERDQDKKRRVVDTESGAAAAVEKLEEVTAGTQLGPEEPCEQEDDNRSLRRHTRELSLRRKSKEDPD REARPETHLDEDEDGKKDKRSSRPRSQPRDPAAKRRPKEAEPEQVAPETPEDRDEDEREEKRRKTTRKKL ESHTVPVQSRSERKAAQSKSVIPKINSPKCPECGQHLDDPNLKYQQHPEDAVDEPQMLTSEKLSIYDSTS TWFDTYEDSPMHRFTSFSVYCSRGHLCPVDTGLIEKNVELYFSGCAKAIHDENPSMEGGINGKNLGPINQ WWLSGFDGGEKVLIGFSTAFAEYILMEPSKEYEPIFGLMQEKIYISKIVVEFLQNNPDAVYEDLINKIET TVPPSTINVNRFTEDSLLRHAQFVVSQVESYDEAKDDDETPIFLSPCMRALIHLAGVSLGQRRATRRVMG ATKEKDKAPTKATTTKLVYQIFDTFFSEQIEKYDKEDKENAMKRRRCGVCEVCQQPECGKCKACKDMVKF GGTGRSKQACLKRRCPNLAVKEADDDEEADDDVSEMPSPKKLHQGKKKKQNKDRISWLGQPMKIEENRTY YQKVSIDEEMLEVGDCVSVIPDDSSKPLYLARVTALWEDKNGQMMFHAHWFCAGTDTVLGATSDPLELFL VGECENMQLSYIHSKVKVIYKAPSENWAMEGGTDPETTLPGAEDGKTYFFQLWYNQEYARFESPPKTQPT EDNKHKFCLSCIRLAELRQKEMPKVLEQIEEVDGRVYCSSITKNGVVYRLGDSVYLPPEAFTFNIKVASP VKRPKKDPVNETLYPEHYRKYSDYIKGSNLDAPEPYRIGRIKEIHCGKKKGKVNEADIKLRLYKFYRPEN THRSYNGSYHTDINMLYWSDEEAVVNFSDVQGRCTVEYGEDLLESIQDYSQGGPDRFYFLEAYNSKTKNF EDPPNHARSPGNKGKGKGKGKGKGKHQVSEPKEPEAAIKLPKLRTLDVFSGCGGLSEGFHQAGISETLWA IEMWDPAAQAFRLNNPGTTVFTEDCNVLLKLVMAGEVTNSLGQRLPQKGDVEMLCGGPPCQGFSGMNRFN SRTYSKFKNSLVVSFLSYCDYYRPRFFLLENVRNFVSYRRSMVLKLTLRCLVRMGYQCTFGVLQAGQYGV AQTRRRAIILAAAPGEKLPLFPEPLHVFAPRACQLSVVVDDKKFVSNITRLSSGPFRTITVRDTMSDLPE IQNGASNSEIPYNGEPLSWFQRQLRGSHYQPILRDHICKDMSPLVAARMRHIPLFPGSDWRDLPNIQVRL GDGVIAHKLQYTFHDVKNGYSSTGALRGVCSCAEGKACDPESRQFSTLIPWCLPHTGNRHNHWAGLYGRL EWDGFFSTTVTNPEPMGKQGRVLHPEQHRVVSVRECARSQGFPDSYRFFGNILDRHRQVGNAVPPPLAKA IGLEIKLCLLSSARESASAAVKAKEEAATKD TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_001199432 |
ORF Size | 4503 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001199432.1, NP_001186361.1 |
RefSeq Size | 5251 bp |
RefSeq ORF | 4506 bp |
Locus ID | 13433 |
UniProt ID | P13864 |
MW | 170.4 kDa |
Gene Summary | This gene encodes a methyltransferase that preferentially methylates cytosines of CpG residues in hemimethylated DNA to generate fully methylated CpG base pairs during DNA replication. This enzyme plays roles in diverse cellular processes including cell cycle regulation, DNA repair, and telomere maintenance. The encoded protein is composed of an N-terminal domain with a nuclear localization sequence and replication fork-targeting domain, a DNA-binding CXXC domain, two bromo-adjacent homology domains, and a C-terminal catalytic domain. Mouse embryonic stem cells mutant for this gene are viable, but when introduced into the germ line, cause a recessive lethal phenotype with mutant embryos displaying stunted growth and developmental defects. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2015] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC229609 | Dnmt1 (untagged) - Mouse DNA methyltransferase (cytosine-5) 1 (Dnmt1), transcript variant 3 |
CNY 14,350.00 |