Scn9a (NM_001290674) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	Myc-DDK
Synonyms	mKIAA4197; Nav1.7; PN1
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MR231900 ORF sequence Red=Cloning site Blue=ORF Green=Tags(s) CTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCCGGCGC GCCC ATGGCGATGTTGCCTCCGCCAGGACCTCAGAGCTTCGTTCACTTCACAAAACAGTCCCTTGCCCTCATTG AACAACGCATTTCTGAAGAAAAAGCCAAGGGACACAAAGATGAAAAGAAAGATGATGAGGAAGAAGGTCC CAAGCCCAGTAGTGACTTGGAAGCAGGTAAACAGCTACCCTTCATCTACGGAGACATTCCCCCGGGAATG GTGTCAGAGCCCCTGGAGGACCTGGACCCATACTATGCAGACAAAAAAACTTTTATAGTATTGAACAAAG GGAAAGCAATCTTCCGTTTCAACGCCACCCCTGCTTTGTACATGCTGTCTCCCTTCAGTCCTCTCAGAAG AATATCTATTAAGATTTTAGTGCACTCCTTATTCAGCATGCTAATCATGTGCACAATTCTGACAAACTGC ATATTCATGACCATGAGCAACCCTCCAGATTGGACCAAAAACGTAGAGTACACTTTTACTGGGATATATA CTTTTGAATCACTCATAAAAATCCTTGCAAGAGGCTTTTGCGTGGGCGAATTCACCTTCCTCCGTGACCC TTGGAACTGGCTGGACTTTGTTGTCATTGTTTTTGCGTATTTAACAGAATTTGTAAACCTAGGCAATGTT TCAGCTCTTCGAACTTTCAGAGTCTTGAGAGCTTTGAAAACTATTTCTGTAATTCCAGGACTAAAAACCA TCGTGGGGGCCCTGATCCAATCAGTGAAGAAGCTCTCTGACGTCATGATCCTCACTGTGTTCTGTCTCAG TGTGTTCGCACTAATTGGACTACAACTGTTTATGGGCAACTTGAAGCATAAATGTTTCCGGAAGGACCTT GAGCAGAATGAAACATTAGAAAGCATCATGAGTACTGCTGAGAGTGAAGAAGAATTGAAAAGATATTTTT ATTACTTGGAGGGATCCAAAGATGCTCTTCTTTGCGGTTTCAGCACAGATTCAGGGCAGTGTCCTGAAGG GTACGAGTGTGTGACAGCTGGCAGAAACCCAGATTATGGCTACACAAGCTTTGACACGTTCGGCTGGGCC TTCTTGGCCTTGTTTCGGCTAATGACTCAGGACTACTGGGAGAACCTTTATCAACAGACACTGCGTGCTG CTGGCAAAACCTACATGATTTTCTTTGTCGTGGTGATATTTCTGGGATCCTTTTACCTGATAAACTTGAT CCTGGCTGTGGTAGCCATGGCGTACGAGGAACAGAACCAGGCCAACATCGAAGAAGCTAAACAGAAAGAG TTAGAATTTCAGCAGATGTTAGACCGACTCAAAAAAGAGCAGGAAGAAGCCGAGGCGATCGCTGCAGCCG CTGCTGAGTACACGAGTTTAGGGCGGAGCAGGATTATGGGACTCTCTGAGAGCTCTTCAGAAACCTCCAG GCTGAGCTCAAAGAGTGCCAAGGAGAGAAGAAACCGAAGAAAGAAAAAAAAACAGAAGCTGTCCAGTGGC GAGGAAAAGGGTGATGATGAGAAGCTGTCCAAGTCAGGGTCAGAGGAAAGCATCCGAAAGAAAAGCTTCC ATCTCGGCGTGGAAGGGCACCACCGGGCCAGGGAAAAGAGGCTGTCCACCCCCAACCAGTCACCACTCAG CATTCGTGGGTCCTTGTTTTCTGCCAGGCGCAGCAGCAGAACAAGTCTCTTCAGTTTTAAGGGGCGAGGA AGAGATCTGGGATCTGAAACGGAATTTGCTGATGATGAGCATAGCATTTTTGGAGACAACGAGAGCAGAA GGGGTTCACTATTTGTACCCCATAGACCCCGGGAGCGGCGCAGCAGTAACATCAGCCAGGCCAGTAGGTC CCCACCAGTGCTGCCGGTGAACGGGAAGATGCACAGTGCAGTGGACTGCAATGGCGTGGTGTCGCTTGTT GATGGACCCTCAGCCCTCATGCTCCCCAATGGACAGCTTCTTCCAGAGGTGATAATAGATAAGGCAACTT CCGACGACAGCGGCACAACTAATCAGATGCGTAAAAAAAGGCTCTCTAGTTCTTACTTTTTGTCTGAGGA CATGCTGAATGACCCACATCTCAGGCAAAGGGCCATGAGCAGAGCAAGCATTCTAACCAACACAGTAGAA GAACTTGAAGAATCTAGACAAAAATGTCCACCATGGTGGTACAGATTTGCTCACACATTTTTAATCTGGA ATTGTTCTCCATATTGGATAAAATTCAAAAAGTTCATCTATTTTATTGTAATGGATCCTTTTGTAGATCT TGCAATTACCATTTGCATAGTTTTAAACACCTTGTTTATGGCTATGGAGCACCATCCAATGACGGATGAA TTCAAAAATGTACTTGCAGTCGGGAACCTGGTCTTCACAGGGATCTTCGCAGCTGAAATGGTACTGAAGT TAATAGCCATGGATCCCTATGAATATTTCCAAGTAGGGTGGAATATTTTTGACAGCCTGATTGTGACGTT GAGTTTGGTGGAGCTTTTCCTAGCAGATGTGGAAGGATTATCAGTCCTGCGGTCCTTTAGATTGCTGCGA GTCTTCAAGTTGGCAAAATCCTGGCCCACACTGAATATGCTCATTAAGATCATCGGCAACTCGGTGGGCG CACTGGGCAACCTGACCCTGGTGCTGGCCATCATCGTCTTCATTTTTGCCGTGGTCGGCATGCAGCTGTT TGGAAAGAGCTACAAGGAGTGTGTTTGCAAGATCAACGAGAACTGCAAGCTCCCACGCTGGCACATGAAC GACTTCTTCCACTCCTTCCTGATCGTGTTCCGTGTGCTGTGTGGGGAGTGGATAGAGACCATGTGGGACT GCATGGAGGTTGCGGGCCAGACCATGTGCCTTATTGTTTACATGATGGTCATGGTGATTGGGAACCTTGT GGTCCTGAACCTGTTTCTGGCTTTATTACTGAGTTCCTTTAGTTCTGACAATCTTACAGCAATTGAAGAA GACACCGACGCAAACAACCTCCAGATTGCAGTGGCCAGAATTAAAAGAGGGATCAATTATGTGAAACAGA CCCTGCGTGAATTCATTCTAAAGTCATTTTCCAAAAAGCCAAAGGGCTCCAAGGACACAAAACGAACAGC GGATCCCAACAACAAAAGAGAAAACTATATCTCAAACCGTACCCTTGCGGAGATAAGCAAAGATCACAAT TTCCTCAAAGAAAAGGATAAGATCAGTGGTTTTAGCAGCAGTCTAGACAAAAGCTTTATGGATGAAAACG ATTACCAGTCCTTTATTCATAATCCCAGCCTCACAGTGACAGTGCCCATTGCACCTGGGGAGTCTGATTT GGAGAATATGAACACAGAAGAGCTTAGCAGTGACTCAGATAGTGACTACAGCAAAGAGAGACGGAACCGA TCAAGTTCTTCAGAGTGCAGCACAGTTGATAACCCTCTGCCAGGAGAAGAGGAGGCAGAAGCTGAGCCTA TCAATGCAGATGAGCCCGAAGCCTGTTTTACAGATGGCTGTGTGAGGAGATTTCCATGCTGCCAAGTTAA CATAGACTCCGGGAAAGGGAAAGTTTGGTGGACCATCCGGAAGACCTGCTACAGGATAGTGGAGCACAGC TGGTTTGAAAGCTTCATTGTTCTCATGATCCTGCTCAGCAGTGGAGCTCTGGCTTTTGAGGATATATATA TTGAAAAGAAAAAGACCATTAAGATTATCCTGGAGTATGCTGACAAGATATTCACCTACATCTTCATTCT GGAAATGCTTCTAAAATGGGTGGCATACGGGTATAAAACATATTTCACTAATGCCTGGTGTTGGCTGGAC TTCTTAATTGTTGATGTGTCTCTAGTTACTTTAGTAGCCAACACTCTTGGCTACTCAGACCTTGGCCCCA TTAAATCTCTACGGACACTGAGGGCCCTAAGACCCCTAAGAGCTTTGTCTAGATTTGAAGGAATGAGGGT AGTGGTCAACGCACTCATAGGAGCAATCCCTTCCATCATGAATGTGCTTCTTGTGTGCCTTATATTCTGG CTAATATTTAGCATCATGGGAGTCAATCTGTTTGCTGGCAAGTTCTATGAGTGTGTTAACACCACAGATG GCTCACGATTTTCTGTATCTCAAGTTGCAAACCGTTCTGAGTGTTTTGCCCTGATGAATGTTAGTGGAAA TGTGCGATGGAAAAACCTGAAAGTAAACTTCGATAACGTTGGACTTGGTTACCTGTCGCTGCTTCAAGTT GCAACGTTCAAGGGCTGGATGGATATTATGTATGCAGCAGTTGACTCTGTTAATGTAAATGCACAACCAA TATATGAATACAACCTCTACATGTACATTTATTTTGTCATCTTCATCATCTTTGGCTCATTCTTCACTTT GAACTTGTTCATTGGTGTCATCATCGATAATTTCAACCAACAGAAGAAGAAGCTTGGAGGTCAAGATATC TTTATGACAGAAGAACAGAAGAAATACTATAATGCAATGAAGAAGCTGGGGTCCAAGAAACCACAAAAAC CAATTCCGAGGCCAGGGAACAAATTCCAAGGATGCATATTTGACTTAGTGACAAACCAAGCTTTTGATAT CACCATCATGGTTCTTATCTGCCTCAATATGGTAACCATGATGGTAGAAAAAGAGGGGCAAACTGACTAC ATGAGTTTTGTGCTATACTGGATCAACGTGGTCTTCATCATCCTGTTCACTGGGGAGTGTGTGCTGAAGC TGATCTCTCTCAGGCATTACTACTTCACTGTGGGATGGAACATTTTTGATTTTGTGGTAGTGATCCTCTC CATTGTAGGAATGTTTCTCGCTGAGATGATAGAGAAGTATTTCGTGTCTCCTACCCTGTTCCGAGTCATT CGCCTGGCCAGGATTGGACGAATCCTACGCCTGATCAAAGGCGCCAAGGGGATCCGCACGCTGCTCTTTG CTCTGATGATGTCCCTTCCTGCGCTGTTCAACATCGGCCTCCTGCTTTTCCTCGTCATGTTCATCTACGC CATCTTTGGGATGTCCAACTTTGCCTACGTTAAAAAGGAAGCTGGAATTAATGACATGTTCAACTTTGAG ACCTTCGGCAACAGCATGATCTGCCTGTTCCAAATCACCACCTCTGCGGGCTGGGATGGACTGCTGGCCC CCATCCTCAACAGTGCACCTCCTGACTGTGACCCAAAAAAGGTTCACCCAGGAAGTTCAGTGGAAGGGGA CTGTGGAAATCCATCTGTGGGAATTTTCTACTTTGTCAGCTACATCATCATATCCTTCCTGGTTGTGGTG AACATGTACATTGCTGTCATCCTGGAGAACTTCAGCGTTGCCACAGAAGAAAGTACTGAGCCCCTGAGTG AGGACGACTTTGAGATGTTCTACGAAGTCTGGGAGAAGTTCGACCCTGACGCCACCCAGTTCATAGAGTT CTGCAAGCTCTCTGACTTTGCAGCAGCCCTGGATCCTCCCCTCCTCATCGCAAAGCCAAACAAAGTCCAG CTCATTGCCATGGACCTGCCCATGGTGAGTGGAGACCGCATCCACTGCCTGGACATCTTATTTGCTTTTA CAAAGCGGGTCCTGGGCGAGAGCGGAGAGATGGATTCCCTTCGTTCACAGATGGAAGAAAGGTTTATGTC AGCCAATCCTTCTAAAGTGTCCTATGAGCCCATCACAACCACACTGAAGCGAAAACAAGAGGATGTATCT GCGACTATCATTCAGCGTGCTTACAGACGGTACCGCCTTAGGCAAAACGTCAAGAATATATCAAGTATAT ATATAAAAGATGGAGACAGAGATGACGATTTGCCCAATAAAGAAGATATAGTTTTTGATAATGTTAACGA GAACTCAAGTCCAGAAAAGACAGATGCAACAGCCTCTACCATCTCTCCACCTTCCTATGACAGTGTCACA AAGCCAGATCAAGAGAAATATGAAACAGACAAAACGGAGAAGGAAGACAAAGAGAAAGACGAAAGCAGGA AA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >MR231900 protein sequence Red=Cloning site Green=Tags(s) MAMLPPPGPQSFVHFTKQSLALIEQRISEEKAKGHKDEKKDDEEEGPKPSSDLEAGKQLPFIYGDIPPGM VSEPLEDLDPYYADKKTFIVLNKGKAIFRFNATPALYMLSPFSPLRRISIKILVHSLFSMLIMCTILTNC IFMTMSNPPDWTKNVEYTFTGIYTFESLIKILARGFCVGEFTFLRDPWNWLDFVVIVFAYLTEFVNLGNV SALRTFRVLRALKTISVIPGLKTIVGALIQSVKKLSDVMILTVFCLSVFALIGLQLFMGNLKHKCFRKDL EQNETLESIMSTAESEEELKRYFYYLEGSKDALLCGFSTDSGQCPEGYECVTAGRNPDYGYTSFDTFGWA FLALFRLMTQDYWENLYQQTLRAAGKTYMIFFVVVIFLGSFYLINLILAVVAMAYEEQNQANIEEAKQKE LEFQQMLDRLKKEQEEAEAIAAAAAEYTSLGRSRIMGLSESSSETSRLSSKSAKERRNRRKKKKQKLSSG EEKGDDEKLSKSGSEESIRKKSFHLGVEGHHRAREKRLSTPNQSPLSIRGSLFSARRSSRTSLFSFKGRG RDLGSETEFADDEHSIFGDNESRRGSLFVPHRPRERRSSNISQASRSPPVLPVNGKMHSAVDCNGVVSLV DGPSALMLPNGQLLPEVIIDKATSDDSGTTNQMRKKRLSSSYFLSEDMLNDPHLRQRAMSRASILTNTVE ELEESRQKCPPWWYRFAHTFLIWNCSPYWIKFKKFIYFIVMDPFVDLAITICIVLNTLFMAMEHHPMTDE FKNVLAVGNLVFTGIFAAEMVLKLIAMDPYEYFQVGWNIFDSLIVTLSLVELFLADVEGLSVLRSFRLLR VFKLAKSWPTLNMLIKIIGNSVGALGNLTLVLAIIVFIFAVVGMQLFGKSYKECVCKINENCKLPRWHMN DFFHSFLIVFRVLCGEWIETMWDCMEVAGQTMCLIVYMMVMVIGNLVVLNLFLALLLSSFSSDNLTAIEE DTDANNLQIAVARIKRGINYVKQTLREFILKSFSKKPKGSKDTKRTADPNNKRENYISNRTLAEISKDHN FLKEKDKISGFSSSLDKSFMDENDYQSFIHNPSLTVTVPIAPGESDLENMNTEELSSDSDSDYSKERRNR SSSSECSTVDNPLPGEEEAEAEPINADEPEACFTDGCVRRFPCCQVNIDSGKGKVWWTIRKTCYRIVEHS WFESFIVLMILLSSGALAFEDIYIEKKKTIKIILEYADKIFTYIFILEMLLKWVAYGYKTYFTNAWCWLD FLIVDVSLVTLVANTLGYSDLGPIKSLRTLRALRPLRALSRFEGMRVVVNALIGAIPSIMNVLLVCLIFW LIFSIMGVNLFAGKFYECVNTTDGSRFSVSQVANRSECFALMNVSGNVRWKNLKVNFDNVGLGYLSLLQV ATFKGWMDIMYAAVDSVNVNAQPIYEYNLYMYIYFVIFIIFGSFFTLNLFIGVIIDNFNQQKKKLGGQDI FMTEEQKKYYNAMKKLGSKKPQKPIPRPGNKFQGCIFDLVTNQAFDITIMVLICLNMVTMMVEKEGQTDY MSFVLYWINVVFIILFTGECVLKLISLRHYYFTVGWNIFDFVVVILSIVGMFLAEMIEKYFVSPTLFRVI RLARIGRILRLIKGAKGIRTLLFALMMSLPALFNIGLLLFLVMFIYAIFGMSNFAYVKKEAGINDMFNFE TFGNSMICLFQITTSAGWDGLLAPILNSAPPDCDPKKVHPGSSVEGDCGNPSVGIFYFVSYIIISFLVVV NMYIAVILENFSVATEESTEPLSEDDFEMFYEVWEKFDPDATQFIEFCKLSDFAAALDPPLLIAKPNKVQ LIAMDLPMVSGDRIHCLDILFAFTKRVLGESGEMDSLRSQMEERFMSANPSKVSYEPITTTLKRKQEDVS ATIIQRAYRRYRLRQNVKNISSIYIKDGDRDDDLPNKEDIVFDNVNENSSPEKTDATASTISPPSYDSVT KPDQEKYETDKTEKEDKEKDESRK TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	AscI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001290674
ORF Size	5952 bp
OTI Disclaimer	Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001290674.1, NP_001277603.1
RefSeq Size	9865 bp
RefSeq ORF	5955 bp
Locus ID	20274
UniProt ID	Q62205
MW	225.8 kDa
Gene Summary	Mediates the voltage-dependent sodium ion permeability of excitable membranes. Assuming opened or closed conformations in response to the voltage difference across the membrane, the protein forms a sodium-selective channel through which Na(+) ions may pass in accordance with their electrochemical gradient (PubMed:15123669). It is a tetrodotoxin-sensitive Na(+) channel isoform. Plays a role in pain mechanisms, especially in the development of inflammatory pain (PubMed:15314237).[UniProtKB/Swiss-Prot Function]

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