Flna (NM_001290421) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	Myc-DDK
Synonyms	ABP-280; Dilp2; F730004A14Rik; filamin-1; Fln1; GENA 379
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MR231901 representing NM_001290421 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCTGACATCCGTGAGGCACCCCAGGATTTTCACCCAGACAGGGTGAAGGCACGTGGGCCTGGATTGG AGAAGACTGGTGTGGCTGTCAACAAGCCAGCAGAGTTCACAGTTGATGCCAAGCATGCTGGGAAGGCTCC TCTTCGAGTTCAAGTTCAGGACAATGAGGGCTGCTCTGTGGAAGCGACAGTCAAGGACAATGGCAATGGT ACTTACAGCTGTTCTTATGTGCCCAGAAAGCCAGTGAAGCACACAGCCATGGTTTCTTGGGGAGGTGTCA GCATCCCCAACAGTCCTTTCCGGGTGAATGTGGGAGCTGGCAGCCATCCAAACAAAGTCAAGGTGTATGG TCCAGGAGTGGCCAAGACTGGGCTCAAGGCCCATGAACCTACCTACTTTACTGTGGATTGTACTGAAGCT GGCCAGGGAGATGTCAGCATTGGTATCAAGTGTGCCCCTGGAGTAGTGGGCCCCACTGAGGCTGATATTG ACTTTGATATCATCCGCAATGACAATGACACCTTCACTGTAAAATACACACCCTGTGGGGCTGGCAGCTA TACCATCATGGTCCTTTTTGCTGACCAGGCCACACCCACCAGCCCCATCAGAGTCAAAGTGGAGCCTTCT CATGATGCCAGCAAGGTGAAGGCTGAGGGTCCTGGCCTAAATCGCACTGGTGTTGAACTTGGCAAACCCA CCCATTTCACAGTCAATGCTAAAACTGCTGGGAAAGGCAAGCTGGATGTCCAATTCTCAGGACTGGCTAA GGGAGATGCAGTACGGGATGTGGACATCATTGACCACCATGATAATACCTACACAGTCAAGTACATTCCT GTGCAGCAGGGCCCAGTAGGTGTCAATGTCACTTATGGAGGAGATCACATCCCCAAGAGTCCATTTTCAG TGGGAGTATCTCCAAGCCTGGATCTCAGCAAAATCAAGGTGTCTGGCCTTGGTGACAAAGTGGACGTTGG CAAAGATCAAGAGTTCACAGTAAAGTCAAAGGGTGCAGGTGGTCAAGGCAAAGTAGCATCCAAGATTGTG AGTCCCTCAGGTGCAGCGGTACCCTGCAAGGTAGAGCCAGGCCTGGGAGCTGACAACAGCGTGGTACGTT TTGTGCCCCGTGAAGAGGGGCCCTATGAGGTGGAAGTGACCTATGATGGTGTGCCTGTACCTGGCAGTCC CTTTCCACTAGAAGCTGTGGCCCCCACCAAACCCAGCAAGGTGAAGGCGTTTGGACCAGGGCTACAGGGG GGCAATGCAGGCTCCCCTGCCCGCTTCACCATTGATACAAAGGGTGCTGGCACTGGTGGCCTGGGCCTGA CAGTGGAAGGCCCCTGTGAAGCACAGCTTGAGTGCCTAGACAACGGGGATGGTACATGCTCTGTGTCTTA TGTACCCACTGAGCCTGGGGACTACAACATCAACATCCTTTTTGCTGACACCCACATTCCTGGATCCCCA TTCAAGGCCCATGTGGCTCCTTGTTTTGATGCATCCAAGGTGAAGTGCTCAGGCCCTGGGCTGGAGCGGG CTACTGCTGGTGAGGTAGGGCAGTTCCAAGTGGACTGTTCAAGTGCTGGCAGTGCTGAGTTGACGATTGA GATCTGCTCTGAGGCAGGACTGCCAGCTGAAGTATACATTCAAGACCATGGTGATGGCACACACACCATT ACCTATATTCCTCTCTGTCCTGGGGCTTACACTGTTACCATCAAGTATGGCGGCCAGCCTGTGCCCAACT TCCCCAGCAAGCTACAGGTGGAACCTGCTGTAGATACCTCAGGTGTACAGTGCTATGGGCCTGGGATTGA AGGTCAAGGTGTCTTCCGAGAGGCAACCACTGAGTTCAGTGTGGATGCCCGGGCTCTTACACAGACTGGA GGGCCACATGTCAAGGCTCGAGTGGCCAACCCCTCAGGCAATCTGACAGATACCTATGTGCAAGACTGTG GGGATGGCACATACAAAGTGGAATACACTCCATATGAGGAAGGAGTACACTCTGTGGATGTGACTTATGA TGGCAGCCCTGTGCCCAGCAGCCCCTTCCAGGTGCCTGTAACAGAGGGCTGTGACCCCTCCCGGGTGCGT GTCCATGGACCAGGCATCCAAAGTGGTACCACCAACAAACCCAACAAGTTCACAGTAGAAACCAGGGGAG CTGGCACAGGTGGCCTGGGCTTGGCTGTTGAGGGTCCCTCAGAGGCCAAGATGTCTTGTATGGATAATAA AGATGGCAGCTGCTCAGTAGAATACATCCCCTATGAAGCTGGAACCTATAGCCTTAATGTCACTTATGGT GGTCACCAAGTGCCAGGTAGTCCCTTCAAGGTCCCTGTACATGATGTGACAGATGCATCTAAAGTCAAGT GTTCTGGACCTGGCCTAAGCCCAGGCATGGTCCGTGCCAACCTCCCTCAGTCCTTTCAGGTGGACACAAG CAAAGCTGGAGTTGCCCCACTGCAGGTCAAAGTGCAGGGGCCCAAAGGCCTGGTGGAGCCAGTGGATGTA GTGGACAATGCTGATGGTACTCAGACTGTCAACTATGTGCCCAGCCGAGAAGGGTCCTATAGCATTTCTG TGCTGTATGGTGAAGAAGAAGTGCCACGGAGCCCCTTCAAGGTCAAGGTGCTGCCTACACATGATGCCAG TAAGGTGAAGGCCAGTGGACCTGGACTCAACACCACTGGTGTACCTGCTAGCCTGCCTGTGGAGTTCACC ATTGATGCCAAGGATGCTGGGGAGGGTCTGTTGGCTGTCCAGATTACGGATCCTGAAGGCAAGCCCAAGA AGACACACATTCAAGATAATCATGATGGCACATACACGGTGGCTTATGTGCCAGATGTGCCAGGCCGGTA CACAATCCTCATCAAGTATGGTGGTGATGAGATTCCCTTTTCCCCGTACCGTGTCCGGGCTGTGCCCACT GGGGATGCCAGCAAGTGCACAGTCACAGTGTCAATCGGAGGTCACGGGCTAGGTGCTGGCATTGGCCCCA CCATCCAGATTGGGGAGGAGACGGTGATTACTGTGGACACAAAAGCAGCAGGCAAAGGCAAAGTGACTTG TACTGTGTGCACACCTGATGGCTCAGAGGTAGACGTGGACGTGGTGGAGAATGAGGATGGCACCTTTGAC ATCTTCTACACAGCTCCCCAACCGGGCAAATATGTCATCTGTGTGCGCTTCGGTGGCGAGCATGTGCCCA ACAGCCCCTTCCAAGTTACAGCTTTGGCTGGGGACCAACCAACAGTGCAGACCCCATTAAGGTCTCAGCA GCTGGCTCCACAGTATAACTATCCTCAGGGTAGCCAGCAAACCTGGATTCCAGAGAGGCCCATGGTGGGC GTTAATGGGCTGGATGTGACCAGCCTGAGGCCCTTTGATCTTGTCATCCCCTTCACTATCAAGAAGGGGG AGATCACTGGGGAAGTTCGAATGCCCTCAGGCAAGGTGGCCCAGCCTTCCATTACTGATAACAAAGATGG CACTGTTACTGTACGTTACTCACCCAGTGAAGCTGGCCTGCATGAAATGGACATTCGCTATGACAATATG CATATCCCAGGAAGCCCTCTGCAGTTCTATGTTGATTATGTCAACTGTGGCCACATCACTGCTTATGGTC CTGGCCTTACCCATGGAGTGGTCAACAAACCTGCCACCTTCACTGTCAACACCAAGGATGCAGGAGAGGG GGGCTTGTCTCTGGCCATTGAGGGTCCATCTAAAGCAGAAATCAGTTGCACTGACAACCAGGATGGAACA TGCAGTGTCTCTTACCTGCCTGTACTGCCTGGTGACTATAGCATCCTAGTTAAGTACAATGATCAACACA TCCCAGGCAGTCCCTTTACTGCCAGAGTAACAGGTGACGATTCCATGCGTATGTCCCACCTAAAGGTGGG TTCTGCTGCTGATATCCCCATCAATATCTCAGAAACAGACCTTAGCCTACTCACAGCCACTGTGGTGCCA CCTTCGGGTCGAGAGGAACCCTGTCTGCTGAAACGTTTGCGAAATGGCCACGTGGGGATTTCCTTCGTGC CCAAGGAGACAGGGGAGCACCTGGTACATGTGAAGAAGAATGGCCAGCATGTGGCAAGCAGTCCCATCCC AGTAGTGATCAGCCAGTCGGAGATAGGTGATGCCAGCCGTGTGAGGGTCTCTGGTCAAGGTCTTCATGAA GGTCATACCTTTGAGCCTGCAGAGTTTATTATTGACACCAGAGATGCAGGCTACGGTGGGCTTAGTCTGT CCATTGAGGGCCCTAGCAAAGTAGACATCAACACAGAGGATCTGGAGGATGGCACATGCAGGGTCACCTA CTGTCCCACAGAGCCTGGAAACTACATTATAAACATCAAATTTGCTGACCAGCATGTGCCTGGCAGTCCC TTTTCTGTGAAGGTGACAGGTGAGGGCCGGGTGAAAGAGAGTATCACACGCAGGCGACGTGCCCCTTCTG TGGCCAATATTGGCAGTCATTGTGACCTCAGCCTGAAGATTCCTGAAATTAGCATCCAAGATATGACAGC CCAGGTGACCAGCCCATCAGGCAAGACCCATGAGGCAGAGATCGTAGAAGGAGAGAACCATACTTACTGT ATCCGATTTGTGCCTGCTGAGATGGGAATGCATACAGTCAGTGTCAAGTACAAGGGCCAGCATGTACCTG GGAGCCCCTTCCAGTTCACTGTGGGGCCTCTGGGGGAAGGGGGTGCTCACAAGGTCCGTGCTGGAGGCCC TGGCCTAGAGAGGGCTGAAGTTGGAGTGCCAGCGGAGTTCGGCATTTGGACTAGGGAAGCTGGCGCTGGA GGCCTGGCCATTGCTGTTGAAGGCCCCAGCAAGGCTGAGATCTCTTTCGAGGACCGAAAGGATGGCTCCT GTGGTGTGGCCTACGTAGTTCAGGAGCCAGGTGACTATGAGGTCTCAGTCAAGTTCAACGAGGAGCACAT ACCTGATAGCCCCTTCGTGGTGCCTGTGGCTTCTCCGTCTGGTGACGCCCGCCGCCTTACTGTTTCTAGT CTTCAGGAGTCAGGGTTAAAGGTCAACCAGCCAGCATCTTTTGCAGTCAGTCTGAATGGAGCCAAGGGGG CAATTGATGCCAAGGTGCACAGCCCCTCAGGAGCTCTGGAGGAGTGCTATGTCACAGAGATTGACCAAGA TAAGTATGCTGTGCGTTTCATCCCACGAGAGAATGGCATCTACTTGATTGATGTCAAGTTCAATGGTACT CACATTCCTGGAAGTCCCTTCAAGATCCGAGTTGGGGAGCCTGGGCATGGAGGGGACCCAGGCTTAGTGT CCGCCTATGGAGCAGGCCTGGAAGGTGGTGTCACAGGGAGCCCAGCAGAGTTTATTGTGAACACAAGCAA TGCAGGAGCTGGTGCCCTTTCGGTTACCATTGATGGCCCCTCCAAGGTGAAGATGGATTGCCAGGAGTGC CCCGAGGGCTATCGTGTCACCTATACCCCCATGGCACCTGGCAGCTACCTCATCTCCATCAAGTATGGTG GCCCCTATCACATTGGTGGAAGTCCCTTTAAAGCCAAGGTCACAGGTCCTCGTCTTGTAAGCAACCACAG CCTCCATGAGACATCATCTGTGTTTGTGGACTCTTTGACTAAAGTTGCCACGGTTCCCCAGCATGCAACC TCAGGCCCAGGTCCTGCTGATGTCAGCAAGGTAGTAGCCAAAGGCCTGGGGCTAAGCAAGGCTTATGTAG GCCAGAAGAGCAACTTCACAGTAGATTGCAGCAAAGCAGGTAACAACATGCTGCTGGTGGGCGTGCATGG CCCAAGGACACCCTGTGAAGAGATCCTGGTGAAACACATGGGCAGCCGCCTCTATAGTGTCTCTTACCTG CTCAAGGACAAAGGGGAGTACACACTGGTGGTCAAGTGGGGTGATGAGCATATCCCAGGCAGCCCATACC GCATTATGGTACCC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >MR231901 representing NM_001290421 Red=Cloning site Green=Tags(s) MADIREAPQDFHPDRVKARGPGLEKTGVAVNKPAEFTVDAKHAGKAPLRVQVQDNEGCSVEATVKDNGNG TYSCSYVPRKPVKHTAMVSWGGVSIPNSPFRVNVGAGSHPNKVKVYGPGVAKTGLKAHEPTYFTVDCTEA GQGDVSIGIKCAPGVVGPTEADIDFDIIRNDNDTFTVKYTPCGAGSYTIMVLFADQATPTSPIRVKVEPS HDASKVKAEGPGLNRTGVELGKPTHFTVNAKTAGKGKLDVQFSGLAKGDAVRDVDIIDHHDNTYTVKYIP VQQGPVGVNVTYGGDHIPKSPFSVGVSPSLDLSKIKVSGLGDKVDVGKDQEFTVKSKGAGGQGKVASKIV SPSGAAVPCKVEPGLGADNSVVRFVPREEGPYEVEVTYDGVPVPGSPFPLEAVAPTKPSKVKAFGPGLQG GNAGSPARFTIDTKGAGTGGLGLTVEGPCEAQLECLDNGDGTCSVSYVPTEPGDYNINILFADTHIPGSP FKAHVAPCFDASKVKCSGPGLERATAGEVGQFQVDCSSAGSAELTIEICSEAGLPAEVYIQDHGDGTHTI TYIPLCPGAYTVTIKYGGQPVPNFPSKLQVEPAVDTSGVQCYGPGIEGQGVFREATTEFSVDARALTQTG GPHVKARVANPSGNLTDTYVQDCGDGTYKVEYTPYEEGVHSVDVTYDGSPVPSSPFQVPVTEGCDPSRVR VHGPGIQSGTTNKPNKFTVETRGAGTGGLGLAVEGPSEAKMSCMDNKDGSCSVEYIPYEAGTYSLNVTYG GHQVPGSPFKVPVHDVTDASKVKCSGPGLSPGMVRANLPQSFQVDTSKAGVAPLQVKVQGPKGLVEPVDV VDNADGTQTVNYVPSREGSYSISVLYGEEEVPRSPFKVKVLPTHDASKVKASGPGLNTTGVPASLPVEFT IDAKDAGEGLLAVQITDPEGKPKKTHIQDNHDGTYTVAYVPDVPGRYTILIKYGGDEIPFSPYRVRAVPT GDASKCTVTVSIGGHGLGAGIGPTIQIGEETVITVDTKAAGKGKVTCTVCTPDGSEVDVDVVENEDGTFD IFYTAPQPGKYVICVRFGGEHVPNSPFQVTALAGDQPTVQTPLRSQQLAPQYNYPQGSQQTWIPERPMVG VNGLDVTSLRPFDLVIPFTIKKGEITGEVRMPSGKVAQPSITDNKDGTVTVRYSPSEAGLHEMDIRYDNM HIPGSPLQFYVDYVNCGHITAYGPGLTHGVVNKPATFTVNTKDAGEGGLSLAIEGPSKAEISCTDNQDGT CSVSYLPVLPGDYSILVKYNDQHIPGSPFTARVTGDDSMRMSHLKVGSAADIPINISETDLSLLTATVVP PSGREEPCLLKRLRNGHVGISFVPKETGEHLVHVKKNGQHVASSPIPVVISQSEIGDASRVRVSGQGLHE GHTFEPAEFIIDTRDAGYGGLSLSIEGPSKVDINTEDLEDGTCRVTYCPTEPGNYIINIKFADQHVPGSP FSVKVTGEGRVKESITRRRRAPSVANIGSHCDLSLKIPEISIQDMTAQVTSPSGKTHEAEIVEGENHTYC IRFVPAEMGMHTVSVKYKGQHVPGSPFQFTVGPLGEGGAHKVRAGGPGLERAEVGVPAEFGIWTREAGAG GLAIAVEGPSKAEISFEDRKDGSCGVAYVVQEPGDYEVSVKFNEEHIPDSPFVVPVASPSGDARRLTVSS LQESGLKVNQPASFAVSLNGAKGAIDAKVHSPSGALEECYVTEIDQDKYAVRFIPRENGIYLIDVKFNGT HIPGSPFKIRVGEPGHGGDPGLVSAYGAGLEGGVTGSPAEFIVNTSNAGAGALSVTIDGPSKVKMDCQEC PEGYRVTYTPMAPGSYLISIKYGGPYHIGGSPFKAKVTGPRLVSNHSLHETSSVFVDSLTKVATVPQHAT SGPGPADVSKVVAKGLGLSKAYVGQKSNFTVDCSKAGNNMLLVGVHGPRTPCEEILVKHMGSRLYSVSYL LKDKGEYTLVVKWGDEHIPGSPYRIMVP TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001290421
ORF Size	5964 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001290421.1, NP_001277350.1
RefSeq Size	8369 bp
RefSeq ORF	5967 bp
Locus ID	192176
MW	209.6 kDa
Gene Summary	Actin binding protein that promotes orthogonal branching of actin filaments and links actin filaments to membrane glycoproteins. Anchors various transmembrane proteins to the actin cytoskeleton and serves as a scaffold for a wide range of cytoplasmic signaling proteins (By similarity). Interaction with FLNA may allow neuroblast migration from the ventricular zone into the cortical plate. Tethers cell surface-localized furin, modulates its rate of internalization and directs its intracellular trafficking. Involved in ciliogenesis. Plays a role in cell-cell contacts and adherens junctions during the development of blood vessels, heart and brain organs (PubMed:17172441). Plays a role in platelets morphology through interaction with SYK that regulates ITAM- and ITAM-like-containing receptor signaling, resulting in by platelet cytoskeleton organization maintenance (PubMed:20713593).[UniProtKB/Swiss-Prot Function]

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