Myh14 (NM_001271538) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	Myc-DDK
Synonyms	2400004E04Rik; II-C; NHMCII; NMHC II-C
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MR231906 representing NM_001271538 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCTGCAGTGACCATGTCCGTGTCTGGGAGGAAGGTAGCCTCCAGGCCAGGCCCGGTGCCTGAGGCAG CCCAATCGTTCCTCTACGCGCCCCGGACGCCAAATGTAGGTGGCCCTGGAGGGCCACAGGTGGAGTGGAC AGCCCGGCGCATGGTGTGGGTGCCCTCGGAACTGCATGGGTTCGAGGCAGCAGCCCTGCGGGATGAAGGG GAGGAGGAGGCAGAAGTGGAGCTGGCGGAGAGTGGGCGCCGCCTGCGGCTGCCCAGGGACCAGATCCAGC GCATGAACCCACCCAAGTTCAGCAAGGCAGAAGATATGGCTGAGCTCACCTGCCTCAACGAGGCCTCGGT CCTGCACAACCTGCGAGAACGCTACTACTCCGGGCTCATTTATACCTACTCTGGCCTCTTCTGTGTGGTC ATTAACCCATACAAGCAGCTGCCCATCTACACGGAGGCCATTGTTGAAATGTACCGGGGCAAGAAGCGCC ATGAGGTGCCACCTCACGTGTATGCTGTGACGGAGGGCGCGTACCGCAGCATGCTTCAGGATCGTGAGGA TCAATCCATTCTCTGCACGGGAGAGTCTGGCGCTGGGAAGACGGAGAACACCAAGAAGGTCATCCAGTAC CTGGCCCATGTGGCATCATCTCCAAAGGGCAGGAAGGAGCCTGGTGTCCCTGGGGAGCTAGAGCGTCAGC TTCTTCAAGCCAACCCCATCCTAGAAGCCTTTGGCAATGCCAAGACGGTGAAGAACGACAACTCTTCCCG ATTTGGCAAATTCATCCGCATCAACTTTGATATTGCTGGCTACATCGTGGGAGCAAACATCGAGACCTAT CTGTTGGAGAAGTCCCGGGCCATCAGACAGGCCAAGGATGAATGCAGCTTCCATATCTTCTACCAGCTGC TAGGGGGCGCTGGGGAGCAGCTAAAAGCTGACCTCCTTCTGGAGCCCTGTTCCCATTATCGCTTCCTGAC CAATGGGCCCTCATCGTCCCCGGGCCAGGAGCGTGAGTTATTCCAGGAGACCCTGGAGTCCCTGCGTGTG CTGGGCCTCCTCCCAGAAGAGATCACTGCCATGCTGCGCACTGTCTCTGCTGTCCTCCAGTTTGGCAACA TTGTCCTGAAGAAAGAGCGCAATACGGACCAAGCCACCATGCCTGACAACACAGCTGCCCAGAAGCTTTG CCGCCTCTTGGGACTCGGAGTGACCGACTTCTCCAGAGCCCTTCTCACACCCCGCATCAAAGTGGGCCGA GATTATGTTCAGAAAGCACAAACCAAGGAGCAGGCTGACTTTGCGCTGGAGGCTCTGGCCAAAGCTACCT ATGAGCGCCTGTTCCGCTGGCTGGTTCTGCGGCTCAACCGTGCCCTGGACAGAAGCCCGCGGCAGGGTGC CTCCTTCCTGGGCATCCTGGACATCGCGGGCTTTGAGATCTTCCAGCTGAACTCCTTCGAGCAGCTGTGC ATCAACTACACCAACGAGAAGCTACAGCAGCTATTCAACCACACCATGTTCGTGCTGGAGCAGGAGGAGT ACCAGCGAGAGGGCATCCCCTGGACCTTCCTAGACTTCGGGTTGGACCTGCAACCTTGCATCGACCTCAT TGAGCGTCCGGCCAACCCTCCAGGTCTCCTGGCCCTGCTGGACGAGGAGTGCTGGTTCCCCAAGGCCACG GACAAGTCTTTTGTGGAGAAGGTTGCCCAGGAGCAGGGCAGCCACCCCAAGTTCCAGCGCCCCAGGAACC TGCGAGATCAGGCCGACTTCAGCGTCCTGCACTATGCCGGCAAGGTTGACTACAAAGCCAGTGAGTGGCT GATGAAGAACATGGACCCACTGAATGACAATGTGGCCGCCTTGCTTCACCAGAGCACGGATCGTCTCACA GCTGAGATCTGGAAGGATGAACAAGGGGGTCTCCAACAGTTCACTTTACTTGGCTCCTTCCCATCGCCGT CCCCTGGACCTGCAGGGAGACTCGGCTCTGGCGCTTCTCCTCCAGGGGTTGGGTCTCTCTGTGCACCCAC GGTGGAGGGCATCGTGGGGCTGGAGCAAGTAAGCAGCCTTGGAGATGGCCCACCGGGAGGCCGCCCCCGC CGTGGAATGTTCCGGACTGTGGGGCAGCTCTACAAAGAATCCCTGAGCCGCCTCATGGCCACGCTCAGCA ACACCAACCCTAGTTTTGTCCGCTGCATCGTTCCCAATCATGAGAAGAGGGCTGGAAAGCTGGAGCCGCG CCTGGTGCTGGACCAACTCCGTTGTAACGGGGTCCTCGAGGGTATCCGCATCTGTCGCCAAGGCTTCCCC AACCGCATCCTCTTCCAGGAGTTCCGACAGCGCTATGAAATCCTCACCCCGAACGCTATTCCCAAGGGCT TCATGGACGGCAAACAGGCCTGTGAGAAGATGATCCAGGCCCTGGAGCTAGACCCCAACCTGTACCGTGT TGGCCAAAGCAAGATCTTCTTCCGGGCAGGGGTCCTGGCCCAGCTGGAGGAGGAGCGGGACCTGAAAGTC ACCGACATCATAGTGTCTTTCCAGGCAGCGGCACGGGGCTACCTGGCCCGTAGGGCTTTCCAGAGACGGC AGCAGCAGCAGAGTGCTCTGAGGGTGATGCAGAGAAACTGTGCTGCCTACCTCAAGCTCAGGAACTGGCA GTGGTGGAGGCTGTTCATCAAGGTGAAGCCCCTGCTGCAGGTGACACGGCAGGATGAGGTGCTGCAGGCG CGCGCCCAGGAGCTGCAGAAAGTTCAGGAGCTGCAGCAGCAGAGCGCTCGTGAAGTGGGGGAACTGCAGG GTCGAGTGGCACAGCTAGAGGAGGAGCGCACGCGCCTGGCTGAGCAGCTTCGAGCAGAAGCCGAGCTCTG CTCTGAGGCCGAGGAGACGCGGGCGCGACTGGCTGCCCGGAAGCAGGAGCTGGAGCTGGTGGTGACAGAG CTGGAGGCACGAGTGGGCGAGGAAGAAGAGTGCAGCCGGCAGCTGCAGAGTGAGAAGAAGAGGCTGCAGC AGCATATCCAGGAGCTAGAGAGCCACCTGGAAGCTGAGGAGGGTGCCCGGCAGAAGCTACAGCTGGAGAA GGTGACCACAGAGGCCAAGATGAAGAAATTTGAGGAGGACCTGCTGCTCCTGGAGGACCAGAATTCCAAG CTGAGCAAGGAGCGGAGGCTGCTGGAGGAGCGGCTGGCTGAGTTCTCCTCACAGGCAGCAGAAGAGGAAG AGAAAGTCAAAAGTCTCAACAAGCTGAGGCTCAAATATGAAGCCACAATCTCAGACATGGAAGACCGGCT GAAGAAGGAGGAGAAGGGACGCCAGGAACTAGAGAAGCTGAAGCGACGGCTGGACGGGGAGAGCTCAGAG CTTCAGGAGCAGATGGTGGAGCAGAAGCAGAGGGCAGAGGAACTGCTCGCACAGCTGGGCCGCAAGGAGG ATGAGCTGCAGGCCGCCCTGCTCAGGGCAGAGGAAGAGGGTGGTGCCCGTGCCCAGTTGCTCAAGTCCCT GCGAGAGGCACAGGCTGGCCTTGCTGAGGCTCAGGAGGACCTGGAAGCTGAGCGGGTAGCCAGGGCCAAG GCGGAGAAGCAGCGCCGGGACCTGGGCGAGGAGTTGGAGGCCCTACGTGGGGAGCTCGAGGACACTCTGG ATTCCACCAACGCCCAGCAGGAGCTGCGGTCCAAGAGGGAGCAGGAGGTGACAGAGCTGAAGAAAGCATT GGAAGAGGAGTCCCGTGCCCATGAGGTGTCCATGCAGGAGCTGAGACAGAGGCATAGCCAGGCACTGGTG GAGATGGCCGAGCAGTTGGAGCAAGCCCGGAGGGGCAAAGGTGTGTGGGAGAAGACTCGGCTATCCCTGG AGGCTGAGGTGTCCGAGCTGAAGGCCGAGCTGAGCAGCCTGCAGACCTCGAGACAGGAGGGTGAGCAGAA GAGGCGCCGCCTGGAGTCCCAGCTACAGGAGGTCCAGGGCCGATCCAGTGATTCGGAGCGGGCTCGGTCT GAGGCTGCTGAGAAGCTGCAGAGAGCCCAGGCGGAACTTGAGAGCGTGTCCACAGCCCTGAGTGAGGCGG AGTCCAAAGCCATCAGGCTGGGCAAGGAGCTGAGCAGTGCAGAGTCCCAGCTGCATGACACCCAGGAACT GCTTCAGGAGGAGACCAGGGCAAAGCTGGCCTTGGGGTCCCGTGTGCGTGCCCTAGAGGCCGAGGCGGCG GGGCTTCGGGAGCAGATGGAAGAGGAGGTGGTTGCCAGGGAACGGGCTGGCCGGGAGCTGCAGAGCACGC AGGCCCAGCTCTCTGAATGGCGGCGCCGCCAGGAAGAAGAGGCCGCGGTGCTGGAGGCTGGGGAGGAGGC TCGGCGCCGTGCAGCCCGGGAGGCAGAGACCCTGACCCAGCGCCTGGCAGAAAAGACTGAGGCTGTAGAA CGACTGGAGCGAGCCCGGCGCCGACTGCAGCAGGAGTTGGACGATGCCACTGTGGATCTGGGGCAGCAGA AGCAGCTCCTGAGCACACTGGAGAAGAAGCAGCGGAAATTTGACCAGCTCCTGGCAGAGGAGAAGGCTGC AGTTCTACGGGCTGTGGAAGACCGTGAACGGATAGAGGCCGAAGGCCGGGAGCGAGAGGCCCGGGCCCTG TCGCTGACCCGGGCCCTGGAAGAGGAGCAGGAGGCCCGGGAGGAGCTGGAGAGGCAGAACCGTGCTCTGA GGGCTGAGCTGGAAGCACTGCTGAGCAGCAAGGATGACGTGGGCAAGAACGTGCACGAGCTGGAGCGAGC CCGTAAGGCGGCTGAACAGGCAGCCAGTGACCTGCGGACACAGGTGACAGAATTGGAGGATGAGCTGACA GCCGCAGAGGATGCCAAGCTGCGCCTGGAGGTGACTGTGCAGGCTCTGAAGGCTCAACATGAACGCGACC TGCAGGGCCGCGATGATGCCGGTGAGGAGAGGCGGAGGCAGCTGGCCAAGCAGCTAAGAGACGCAGAGGT AGAGCGCGATGAGGAACGGAAGCAGAGGGCACTGGCTATGGCTGCCCGCAAGAAGCTGGAGCTGGAACTG GAGGAGTTGAAGGCGCAGACATCTGCTGCTGGGCAGGGCAAGGAAGAGGCAGTGAAGCAGCTGAAGAAGA TGCAGGTCCAGATGAAGGAGCTGTGGCGGGAGGTAGAGGAGACGCGTAGCTCCCGCGACGAGATGTTTAC CCTGAGCAGGGAAAATGAGAAGAAGCTCAAGGGGCTGGAAGCTGAGGTGCTGCGTCTGCAAGAGGAACTT GCTGCCTCAGACCGAGCCCGGAGGCAGGCCCAGCAAGACAGAGACGAGATGGCAGAGGAGGTGGCCAGTG GCAATCTTAGCAAGGCAGCCACCCTGGAGGAAAAACGGCAGCTGGAGGGGCGACTGAGCCAGTTGGAAGA GGAGCTGGAGGAAGAACAGAACAACTCGGAGCTGCTCAAGGACCATTACCGAAAGCTAGTGCTACAGGTC GAGTCCCTCACCACAGAACTGTCTGCCGAACGAAGTTTCTCAGCCAAGGCCGAGAGTGGACGGCAGCAGC TGGAGCGGCAGATCCAGGAACTGCGGGCCCGCTTGGGTGAAGAGGATGCTGGAGCCCGAGCCAGGCAGAA AATGCTGATCGCTGCTCTGGAGTCTAAACTGGCCCAGGCAGAGGAGCAGCTGGAGCAGGAGAGCAGGGAG CGCATCCTCTCTGGCAAGCTGGTACGCAGAGCTGAGAAGCGGCTGAAGGAGGTAGTTCTTCAGGTGGATG AAGAGCGCAGGGTGGCTGACCAGGTCCGGGACCAGCTGGAGAAAAGCAACCTCCGGCTGAAGCAGCTCAA GAGGCAGCTGGAGGAGGCAGAGGAGGAGGCATCTCGGGCACAGGCTGGTCGGAGGCGGCTGCAGCGGGAG CTGGAGGACGTCACTGAGTCTGCAGAATCCATGAACCGGGAGGTGACCACGCTGAGGAACAGGCTCCGGC GTGGCCCACTTACATTCACCACACGGACTGTGCGCCAGGTGTTCCGGCTGGAAGAGGGCGTGGCTTCTGA CGAGGAAGAGGCTGAAGGAGCTGAACCTGGCTCTGCACCAGGCCAGGAGCCGGAGGCTCCGCCCCCTGCC ACACCCCAA AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC TGGATTACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >MR231906 representing NM_001271538 Red=Cloning site Green=Tags(s) MAAVTMSVSGRKVASRPGPVPEAAQSFLYAPRTPNVGGPGGPQVEWTARRMVWVPSELHGFEAAALRDEG EEEAEVELAESGRRLRLPRDQIQRMNPPKFSKAEDMAELTCLNEASVLHNLRERYYSGLIYTYSGLFCVV INPYKQLPIYTEAIVEMYRGKKRHEVPPHVYAVTEGAYRSMLQDREDQSILCTGESGAGKTENTKKVIQY LAHVASSPKGRKEPGVPGELERQLLQANPILEAFGNAKTVKNDNSSRFGKFIRINFDIAGYIVGANIETY LLEKSRAIRQAKDECSFHIFYQLLGGAGEQLKADLLLEPCSHYRFLTNGPSSSPGQERELFQETLESLRV LGLLPEEITAMLRTVSAVLQFGNIVLKKERNTDQATMPDNTAAQKLCRLLGLGVTDFSRALLTPRIKVGR DYVQKAQTKEQADFALEALAKATYERLFRWLVLRLNRALDRSPRQGASFLGILDIAGFEIFQLNSFEQLC INYTNEKLQQLFNHTMFVLEQEEYQREGIPWTFLDFGLDLQPCIDLIERPANPPGLLALLDEECWFPKAT DKSFVEKVAQEQGSHPKFQRPRNLRDQADFSVLHYAGKVDYKASEWLMKNMDPLNDNVAALLHQSTDRLT AEIWKDEQGGLQQFTLLGSFPSPSPGPAGRLGSGASPPGVGSLCAPTVEGIVGLEQVSSLGDGPPGGRPR RGMFRTVGQLYKESLSRLMATLSNTNPSFVRCIVPNHEKRAGKLEPRLVLDQLRCNGVLEGIRICRQGFP NRILFQEFRQRYEILTPNAIPKGFMDGKQACEKMIQALELDPNLYRVGQSKIFFRAGVLAQLEEERDLKV TDIIVSFQAAARGYLARRAFQRRQQQQSALRVMQRNCAAYLKLRNWQWWRLFIKVKPLLQVTRQDEVLQA RAQELQKVQELQQQSAREVGELQGRVAQLEEERTRLAEQLRAEAELCSEAEETRARLAARKQELELVVTE LEARVGEEEECSRQLQSEKKRLQQHIQELESHLEAEEGARQKLQLEKVTTEAKMKKFEEDLLLLEDQNSK LSKERRLLEERLAEFSSQAAEEEEKVKSLNKLRLKYEATISDMEDRLKKEEKGRQELEKLKRRLDGESSE LQEQMVEQKQRAEELLAQLGRKEDELQAALLRAEEEGGARAQLLKSLREAQAGLAEAQEDLEAERVARAK AEKQRRDLGEELEALRGELEDTLDSTNAQQELRSKREQEVTELKKALEEESRAHEVSMQELRQRHSQALV EMAEQLEQARRGKGVWEKTRLSLEAEVSELKAELSSLQTSRQEGEQKRRRLESQLQEVQGRSSDSERARS EAAEKLQRAQAELESVSTALSEAESKAIRLGKELSSAESQLHDTQELLQEETRAKLALGSRVRALEAEAA GLREQMEEEVVARERAGRELQSTQAQLSEWRRRQEEEAAVLEAGEEARRRAAREAETLTQRLAEKTEAVE RLERARRRLQQELDDATVDLGQQKQLLSTLEKKQRKFDQLLAEEKAAVLRAVEDRERIEAEGREREARAL SLTRALEEEQEAREELERQNRALRAELEALLSSKDDVGKNVHELERARKAAEQAASDLRTQVTELEDELT AAEDAKLRLEVTVQALKAQHERDLQGRDDAGEERRRQLAKQLRDAEVERDEERKQRALAMAARKKLELEL EELKAQTSAAGQGKEEAVKQLKKMQVQMKELWREVEETRSSRDEMFTLSRENEKKLKGLEAEVLRLQEEL AASDRARRQAQQDRDEMAEEVASGNLSKAATLEEKRQLEGRLSQLEEELEEEQNNSELLKDHYRKLVLQV ESLTTELSAERSFSAKAESGRQQLERQIQELRARLGEEDAGARARQKMLIAALESKLAQAEEQLEQESRE RILSGKLVRRAEKRLKEVVLQVDEERRVADQVRDQLEKSNLRLKQLKRQLEEAEEEASRAQAGRRRLQRE LEDVTESAESMNREVTTLRNRLRRGPLTFTTRTVRQVFRLEEGVASDEEEAEGAEPGSAPGQEPEAPPPA TPQ SGPTRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-RsrII      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001271538
ORF Size	6099 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001271538.1, NP_001258467.1
RefSeq Size	6515 bp
RefSeq ORF	6102 bp
Locus ID	71960
UniProt ID	Q6URW6
MW	232.1 kDa
Gene Summary	Cellular myosin that appears to play a role in cytokinesis, cell shape, and specialized functions such as secretion and capping.[UniProtKB/Swiss-Prot Function]

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