Tet1 (NM_001253857) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	Myc-DDK
Synonyms	2510010B09Rik; AA517754; BB001228; Cxxc6; D10Ertd17e; LCX; mKIAA1676
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MR231909 representing NM_001253857 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCTCGGTCCCGCCCCGCAAAGCCTTCCAAATCAGTCAAAACAAAGCTACAGAAAAAGAAAGACATCC AGATGAAGACGAAGACATCAAAGCAGGCTGTCAGACATGGGGCATCAGCGAAGGCAGTGAACCCCGGAAA ACCGAAGCAATTAATTAAAAGAAGAGATGGTAAGAAGGAAACGGAAGACAAAACCCCGACACCGGCGCCG AGTTTTCTGACGCGAGCCGGGGCCGCCCGAATGAATCGGGATCGAAACCAAGTTCTTTTTCAGAACCCAG ACTCCTTAACTTGCAACGGGTTTACAATGGCTCTTCGAAGAACCTCTCTTAGCTGGCGACTCTCCCAGCG CCCAGTAGTCACACCCAAACCTAAGAAAGTACCACCTTCAAAGAAGCAGTGTACACATAATATCCAGGAC GAGCCGGGGGTAAAGCACTCTGAAAATGATTCAGTCCCCAGCCAACACGCTACCGTGTCCCCAGGCACAG AGAATGGTGAACAGAACAGGTGTTTAGTTGAAGGGGAGAGCCAAGAGATAACCCAGTCTTGCCCTGTATT TGAGGAAAGAATAGAAGATACCCAAAGCTGTATTTCCGCATCTGGGAACCTGGAAGCAGAAATTTCTTGG CCACTGGAAGGGACACATTGTGAAGAACTACTTTCTCATCAGACATCTGATAATGAGTGCACTTCCCCTC AAGAGTGTGCTCCATTGCCCCAAAGATCAACTTCCGAAGTTACCTCTCAGAAAAACACTAGCAATCAGTT AGCAGACTTGAGCTCACAAGTAGAGTCTATCAAGCTGTCTGATCCTTCTCCGAATCCTACGGGAAGCGAC CATAATGGCTTTCCAGACTCCAGTTTTAGGATCGTTCCTGAATTGGACCTGAAAACCTGCATGCCTCTTG ATGAGTCTGTGTATCCCACAGCTCTGATAAGATTCATCTTGGCAGGCTCACAGCCGGATGTCTTTGATAC TAAGCCCCAAGAAAAAACCCTTATAACAACCCCAGAACAAGTGGGAAGCCACCCAAATCAAGTTCTGGAT GCCACCTCTGTCCTAGGACAGGCCTTTAGTACTCTTCCACTTCAGTGGGGATTTTCTGGTGCTAACTTAG TTCAAGTTGAGGCCCTGGGCAAGGGTTCAGACTCACCAGAGGATCTTGGTGCTATTACTATGCTAAACCA ACAAGAAACTGTGGCTATGGATATGGATAGGAATGCTACCCCGGACCTGCCTATCTTCCTTCCTAAGCCT CCAAACACAGTTGCTACCTACAGTAGCCCTCTTTTAGGGCCTGAGCCCCACAGCTCTACCTCCTGTGGGC TTGAAGTCCAAGGTGCTACCCCGATTTTAACTTTGGACTCAGGACACACTCCCCAACTCCCACCAAACCC TGAGTCAAGTTCAGTACCTCTGGTAATAGCTGCAAACGGTACGCGAGCTGAGAAGCAGTTTGGTACCAGC CTTTTCCCAGCCGTCCCACAAGGGTTTACAGTCGCAGCCGAGAATGAAGTCCAGCATGCCCCATTAGATC TTACCCAGGGTTCTCAGGCTGCTCCCAGCAAACTGGAAGGAGAGATTTCTCGGGTCAGCATCACTGGCTC AGCTGATGTCAAAGCCACAGCGATGTCCATGCCGGTTACACAGGCCAGTACCTCTTCTCCCCCATGTAAT TCTACGCCGCCAATGGTGGAGAGGAGGAAGCGCAAGGCCTGTGGGGTCTGTGAGCCGTGCCAGCAGAAGG CCAACTGTGGGGAGTGCACCTACTGCAAGAATCGAAAGAACAGCCACCAGATCTGTAAGAAGAGGAAATG CGAGGTGCTCAAAAAGAAGCCAGAGGCCACCTCACAGGCACAGGTTACAAAAGAAAACAAGAGGCCCCAG AGGGAAAAGAAGCCCAAAGTTTTAAAGACAGACTTTAACAACAAACCAGTAAATGGCCCCAAATCAGAGT CCATGGACTGCAGTAGACGTGGTCATGGGGAAGAAGAGCAAAGGTTGGATTTGATCACACATCCCCTTGA AAATGTAAGGAAGAATGCAGGCGGCATGACGGGAATTGAGGTGGAGAAGTGGGCGCCAAACAAGAAATCA CACTTAGCAGAGGGTCAAGTCAAAGGAAGCTGTGATGCCAATTTAACTGGGGTGGAAAACCCCCAGCCCT CTGAAGATGACAAGCAGCAAACCAACCCTTCTCCGACATTTGCCCAGACCATAAGGAACGGCATGAAAAA TGTACACTGTTTACCCACTGACACACACCTTCCGCTCAACAAATTGAATCATGAGGAATTTAGCAAGGCG TTGGGAAATAATTCCTCTAAATTGCTGACTGATCCCTCAAACTGCAAGGATGCCATGAGTGTCACCACCT CCGGGGGGGAGTGTGATCATCTCAAGGGGCCAAGGAACACCCTGCTCTTCCAGAAGCCTGGCTTGAACTG CAGGTCTGGTGCAGAACCCACCATCTTTAATAATCACCCTAATACACACAGCGCTGGGAGTCGACCTCAC CCTCCTGAGAAGGTACCCAACAAAGAACCCAAAGATGGCTCTCCGGTTCAGCCAAGTCTTCTATCCCTAA TGAAGGACAGGAGATTAACGCTGGAACAAGTGGTAGCCATAGAAGCCCTGACTCAGCTCTCAGAAGCGCC CTCAGAGAGCTCCTCCCCATCCAAGCCGGAGAAGGATGAAGAAGCGCATCAGAAAACGGCTAGTCTGCTT AACAGTTGCAAGGCTATCCTTCATTCGGTGAGAAAAGACCTCCAAGACCCAAACGTTCAAGGAAAGGGCC TTCACCATGACACTGTTGTCTTTAATGGCCAGAACAGAACATTCAAGTCACCTGATAGTTTCGCTACTAA CCAAGCACTTATAAAATCACAAGGATATCCAAGTTCACCTACAGCTGAGAAGAAAGGCGCTGCTGGTGGG AGAGCTCCCTTTGATGGTTTTGAGAATTCACATCCATTGCCCATAGAAAGTCACAATCTTGAAAACTGCA GTCAGGTACTAAGCTGCGATCAGAATTTGAGTTCCCATGACCCATCATGCCAGGATGCACCCTACAGTCA AATTGAAGAAGATGTCGCAGCACAATTGACCCAACTTGCATCCACGATTAATCACATCAACGCCGAGGTC AGGAATGCTGAAAGTACACCAGAAAGCCTTGTTGCAAAGAACACAAAGCAAAAACACAGTCAGGAGAAAC GCATGGTACATCAGAAGCCACCCTCTAGTACACAAACTAAACCCAGTGTACCATCAGCAAAGCCAAAGAA GGCCCAGAAAAAGGCAAGAGCGACCCCACACGCAAACAAGCGGAAAAAGAAACCCCCAGCCCGAAGCTCT CAAGAAAATGATCAGAAGAAGCAAGAGCAGCTAGCTATAGAGTATAGTAAAATGCATGACATTTGGATGT CATCTAAGTTTCAAAGGTTTGGCCAGTCTAGTCCACGTAGTTTTCCTGTTCTGTTGAGAAATATTCCTGT CTTTAACCAAATATTAAAGCCCGTGACTCAGAGTAAAACACCCTCACAACATAATGAGTTATTTCCTCCT ATCAATCAGATAAAATTCACAAGAAACCCTGAATTGGCAAAGGAGAAAGTGAAGGTTGAACCATCAGATT CTCTGCCAACATGCCAATTCAAGACAGAATCCGGTGGGCAGACATTTGCAGAGCCGGCAGATAATTCTCA GGGACAGCCCATGGTGAGTGTCAATCAGGAAGCTCACCCTCTGCCCCAGTCCCCACCATCTAACCAGTGT GCTAATATAATGGCTGGTGCTGCCCAAACACAGTTTCATCTAGGCGCTCAAGAGAACCTGGTGCATCAGA TACCACCACCGACGTTGCCTGGTACCTCCCCTGACACACTCTTGCCAGACCCAGCATCAATCCTCAGGAA AGGGAAAGTGTTACATTTCGATGGAATTACAGTTGTTACGGAGAAGCGTGAAGCTCAAACATCAAGCAAT GGACCACTGGGTCCCACCACAGACAGTGCACAGTCAGAATTTAAAGAGAGCATCATGGACTTACTTAGCA AGCCTGCAAAAAACCTGATAGCTGGATTGAAGGAACAGGAAGCTGCACCCTGTGACTGTGATGGAGGTAC ACAAAAAGAAAAAGGCCCATATTATACACACCTTGGGGCAGGACCAAGTGTGGCTGCTGTCAGGGAGCTC ATGGAGACTAGGTTTGGCCAGAAGGGGAAGGCAATCCGGATTGAGAAGATAGTGTTCACGGGGAAGGAAG GGAAGAGCTCTCAGGGCTGCCCGGTCGCCAAGTGGGTGATCAGAAGAAGTGGTCCTGAAGAGAAGCTTAT TTGTTTGGTTCGTGAGCGTGTAGACCATCACTGTTCGACGGCTGTGATAGTTGTCCTTATCCTGCTGTGG GAAGGTATCCCTCGCCTGATGGCTGACCGCCTGTACAAAGAGCTCACTGAGAACTTGAGGTCCTACAGCG GACATCCCACAGACCGAAGATGTACCCTCAACAAAAAGCGTACCTGCACCTGTCAAGGCATCGACCCAAA AACCTGCGGAGCGTCCTTCTCCTTTGGCTGTTCGTGGAGCATGTATTTCAACGGCTGTAAGTTTGGGAGG AGTGAAAACCCCAGAAAATTCAGACTTGCTCCAAACTACCCCTTACATAACTACTATAAGAGAATTACTG GAATGAGTTCTGAAGGAAGTGACGTGAAAACCGGGTGGATCATTCCAGACCGCAAGACCCTCATAAGCAG AGAGGAAAAACAGCTTGAAAAGAATTTACAAGAATTGGCTACAGTATTAGCTCCACTTTACAAGCAGATG GCTCCAGTTGCTTATCAAAATCAGGTGGAATATGAAGAAGTTGCTGGAGACTGTCGACTTGGAAATGAAG AGGGGCGTCCTTTCTCTGGTGTCACCTGTTGCATGGATTTTTGTGCCCATTCTCACAAGGACATTCACAA CATGCACAACGGAAGCACCGTGGTGTGTACGTTGATTCGAGCAGATGGCCGTGACACAAATTGTCCCGAG GATGAACAACTCCACGTCCTGCCACTATACCGGCTTGCAGACACTGATGAATTTGGCTCCGTGGAAGGGA TGAAGGCCAAAATCAAATCTGGGGCCATCCAAGTCAATGGGCCAACCAGGAAGAGGCGACTACGTTTTAC TGAGCCTGTTCCTCGATGTGGGAAGAGGGCCAAAATGAAGCAGAACCACAATAAATCAGGTTCACACAAC ACTAAGAGCTTTTCATCAGCCTCATCTACTTCTCACCTAGTGAAAGACGAATCTACAGACTTCTGTCCCC TGCAGGCTTCCTCCGCAGAAACATCTACCTGTACGTACAGTAAAACAGCCTCAGGTGGGTTTGCAGAAAC AAGTAGTATTCTCCACTGCACAATGCCTTCTGGAGCACACAGTGGTGCTAATGCAGCTGCTGGGGAATGT ACTGGAACGGTGCAGCCTGCCGAGGTGGCTGCTCATCCTCACCAGTCTCTTCCCACAGCCGATTCTCCCG TTCATGCTGAGCCTCTCACTAGTCCATCTGAGCAGCTAACTTCTAACCAGTCAAACCAGCAGCTCCCTCT CCTCAGCAATTCTCAGAAACTGGCTTCCTGTCAGGTGGAAGATGAGCGGCACCCTGAAGCGGATGAGCCT CAGCACCCCGAGGACGATAACTTGCCTCAACTTGATGAATTCTGGTCAGACAGTGAGGAGATCTACGCCG ATCCTTCCTTTGGTGGCGTGGCGATAGCACCCATTCACGGCTCGGTGCTCATTGAGTGCGCTCGGAAGGA GCTTCATGCTACCACCTCTTTGCGCTCCCCCAAACGAGGGGTCCCTTTTCGTGTGTCCCTTGTATTCTAC CAGCACAAAAGCCTAAACAAGCCTAATCATGGTTTTGATATCAACAAAATTAAGTGTAAATGCAAAAAAG TAACGAAAAAAAAGCCCGCAGACCGGGAGTGTCCTGATGTATCCCCCGAAGCCAATTTATCACACCAAAT TCCTTCTCGAGTTGCATCAACCTTAACCCGAGACAATGTTGTTACCGTGTCCCCATACTCTCTCACTCAT GTTGCGGGACCCTACAATCGTTGGGTC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >MR231909 representing NM_001253857 Red=Cloning site Green=Tags(s) MSRSRPAKPSKSVKTKLQKKKDIQMKTKTSKQAVRHGASAKAVNPGKPKQLIKRRDGKKETEDKTPTPAP SFLTRAGAARMNRDRNQVLFQNPDSLTCNGFTMALRRTSLSWRLSQRPVVTPKPKKVPPSKKQCTHNIQD EPGVKHSENDSVPSQHATVSPGTENGEQNRCLVEGESQEITQSCPVFEERIEDTQSCISASGNLEAEISW PLEGTHCEELLSHQTSDNECTSPQECAPLPQRSTSEVTSQKNTSNQLADLSSQVESIKLSDPSPNPTGSD HNGFPDSSFRIVPELDLKTCMPLDESVYPTALIRFILAGSQPDVFDTKPQEKTLITTPEQVGSHPNQVLD ATSVLGQAFSTLPLQWGFSGANLVQVEALGKGSDSPEDLGAITMLNQQETVAMDMDRNATPDLPIFLPKP PNTVATYSSPLLGPEPHSSTSCGLEVQGATPILTLDSGHTPQLPPNPESSSVPLVIAANGTRAEKQFGTS LFPAVPQGFTVAAENEVQHAPLDLTQGSQAAPSKLEGEISRVSITGSADVKATAMSMPVTQASTSSPPCN STPPMVERRKRKACGVCEPCQQKANCGECTYCKNRKNSHQICKKRKCEVLKKKPEATSQAQVTKENKRPQ REKKPKVLKTDFNNKPVNGPKSESMDCSRRGHGEEEQRLDLITHPLENVRKNAGGMTGIEVEKWAPNKKS HLAEGQVKGSCDANLTGVENPQPSEDDKQQTNPSPTFAQTIRNGMKNVHCLPTDTHLPLNKLNHEEFSKA LGNNSSKLLTDPSNCKDAMSVTTSGGECDHLKGPRNTLLFQKPGLNCRSGAEPTIFNNHPNTHSAGSRPH PPEKVPNKEPKDGSPVQPSLLSLMKDRRLTLEQVVAIEALTQLSEAPSESSSPSKPEKDEEAHQKTASLL NSCKAILHSVRKDLQDPNVQGKGLHHDTVVFNGQNRTFKSPDSFATNQALIKSQGYPSSPTAEKKGAAGG RAPFDGFENSHPLPIESHNLENCSQVLSCDQNLSSHDPSCQDAPYSQIEEDVAAQLTQLASTINHINAEV RNAESTPESLVAKNTKQKHSQEKRMVHQKPPSSTQTKPSVPSAKPKKAQKKARATPHANKRKKKPPARSS QENDQKKQEQLAIEYSKMHDIWMSSKFQRFGQSSPRSFPVLLRNIPVFNQILKPVTQSKTPSQHNELFPP INQIKFTRNPELAKEKVKVEPSDSLPTCQFKTESGGQTFAEPADNSQGQPMVSVNQEAHPLPQSPPSNQC ANIMAGAAQTQFHLGAQENLVHQIPPPTLPGTSPDTLLPDPASILRKGKVLHFDGITVVTEKREAQTSSN GPLGPTTDSAQSEFKESIMDLLSKPAKNLIAGLKEQEAAPCDCDGGTQKEKGPYYTHLGAGPSVAAVREL METRFGQKGKAIRIEKIVFTGKEGKSSQGCPVAKWVIRRSGPEEKLICLVRERVDHHCSTAVIVVLILLW EGIPRLMADRLYKELTENLRSYSGHPTDRRCTLNKKRTCTCQGIDPKTCGASFSFGCSWSMYFNGCKFGR SENPRKFRLAPNYPLHNYYKRITGMSSEGSDVKTGWIIPDRKTLISREEKQLEKNLQELATVLAPLYKQM APVAYQNQVEYEEVAGDCRLGNEEGRPFSGVTCCMDFCAHSHKDIHNMHNGSTVVCTLIRADGRDTNCPE DEQLHVLPLYRLADTDEFGSVEGMKAKIKSGAIQVNGPTRKRRLRFTEPVPRCGKRAKMKQNHNKSGSHN TKSFSSASSTSHLVKDESTDFCPLQASSAETSTCTYSKTASGGFAETSSILHCTMPSGAHSGANAAAGEC TGTVQPAEVAAHPHQSLPTADSPVHAEPLTSPSEQLTSNQSNQQLPLLSNSQKLASCQVEDERHPEADEP QHPEDDNLPQLDEFWSDSEEIYADPSFGGVAIAPIHGSVLIECARKELHATTSLRSPKRGVPFRVSLVFY QHKSLNKPNHGFDINKIKCKCKKVTKKKPADRECPDVSPEANLSHQIPSRVASTLTRDNVVTVSPYSLTH VAGPYNRWV TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001253857
ORF Size	6117 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001253857.2
RefSeq Size	14082 bp
RefSeq ORF	6120 bp
Locus ID	52463
MW	223.4 kDa
Gene Summary	Dioxygenase that catalyzes the conversion of the modified genomic base 5-methylcytosine (5mC) into 5-hydroxymethylcytosine (5hmC) and plays a key role in active DNA demethylation. Also mediates subsequent conversion of 5hmC into 5-formylcytosine (5fC), and conversion of 5fC to 5-carboxylcytosine (5caC). Conversion of 5mC into 5hmC, 5fC and 5caC probably constitutes the first step in cytosine demethylation. Methylation at the C5 position of cytosine bases is an epigenetic modification of the mammalian genome which plays an important role in transcriptional regulation. In addition to its role in DNA demethylation, plays a more general role in chromatin regulation. Preferentially binds to CpG-rich sequences at promoters of both transcriptionally active and Polycomb-repressed genes. Involved in the recruitment of the O-GlcNAc transferase OGT to CpG-rich transcription start sites of active genes, thereby promoting histone H2B GlcNAcylation by OGT. Also involved in transcription repression of a subset of genes through recruitment of transcriptional repressors to promoters. Involved in the balance between pluripotency and lineage commitment of cells it plays a role in embryonic stem cells maintenance and inner cell mass cell specification. Plays an essential role in the tumorigenicity of glioblastoma cells. TET1-mediated production of 5hmC acts as a recruitment signal for the CHTOP-methylosome complex to selective sites on the chromosome, where it methylates H4R3 and activates the transcription of genes involved in glioblastomagenesis (PubMed:25284789).[UniProtKB/Swiss-Prot Function]

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