Cnot1 (NM_001205226) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	Myc-DDK
Synonyms	6030411K04Rik; AA815922; D830048B13
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MR231947 representing NM_001205226 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAATCTTGACTCGCTCTCGCTGGCCTTGTCTCAAATCAGCTACCTGGTGGACAATTTAACCAAGAAAA ACTACCGAGCCAGCCAGCAGGAAATACAGCATATTGTGAATCGGCACGGTCCTGAGGCAGACAGGCATTT ATTACGCTGCCTATTTTCACATGTGGATTTCAGTGGCGATGGTAAAAGCAGTGGCAAAGATTTCCATCAG TTTCTGATTCAGGAGTGTGCCTCGCTGATTACAAAGCCAAATTTTATCTCAACATTGTCATATGCCATTG ATAATCCATTGCACTACCAGAAGAGTTTAAAGCCTGCACCCCACTTATTTGCCCAGCTGAGTAAAGTTCT CAAGCTAAGTAAAGTACAGGAGGTCATTTTTGGTCTTGCCCTTTTGAATTCTTCTAGCCCAGATCTCAGG GGTTTTGCTGCCCAGTTTATTAAACAGAAGCTTCCGGATCTTCTGCGTTCTTACATTGACGCAGACGTCA GTGGAAATCAAGAAGGTGGCTTCCAAGATATAGCAATAGAGGTCCTACACCTCCTACTCTCCCATCTCCT CTTTGGGCAGAAGGGAGCCTTTGGAGTTGGACAGGAACAGATAGACGCCTTTCTTAAGACACTACGCAGA GATTTTCCCCAAGAGCGCTGTCCTGTGGTGCTTGCACCACTTTTATACCCTGAAAAACGGGACATTCTAA TGGACAGGATTTTGCCTGATTCTGGAGGGGTAGCTAAAACCATGATGGAGAGCTCGCTAGCTGACTTCAT GCAGGAAGTGGGCTATGGCTTTTGTGCAAGTATTGAAGAATGTCGCAATATAATCATGCAGTTTGGTGTT CGGGAGGTCACAGCTGCCCAGGTTGCCAGGGTTTTGGGAATGATGGCTCGGACTCATTCAGGATTAACAG ACGGTATTCCATTACAGAGCATCTCTGCTCCTGGCAGTGGGATCTGGAGCGACGGAAAAGATAAAAGCGA GGGGGCACAGGCACACACGTGGAATGTCGAGGTTTTGATTGATGTTCTTAAAGAGCTGAACCCGAGTTTG AATTTCAAGGAAGTAACTTATGAACTGGACCATCCTGGATTTCAGATTCGTGACAGCAAAGGACTTCACA ATGTGGTTTATGGCATTCAAAGGGGTCTGGGCATGGAAGTGTTTCCAGTAGATTTTATATATAGGCCTTG GAAACATGCAGAAGGCCAGCTCTCCTTCATCCAGCATTCCCTGATAAACCCAGAGGTCTTCTGTTTTGCT GACTACCCCTGTCACACTGTTGCCACTGATATTTTGAAAGCACCACCAGAGGATGACAATCGAGAAATTG CCACATGGAAAAGCTTGGATTTGATTGAATCTCTGCTGAGGCTTGCAGAGGTTGGGCAGTATGAGCAAGT GAAACAACTCTTCAGCTTCCCTATCAAACACTGTCCAGACATGTTGGTATTGGCCTTACTTCAAATCAAC ACTTCCTGGCACACCCTACGCCATGAACTCATCTCTACTTTGATGCCAATTTTCCTTGGAAACCATCCTA ACTCTGCCATTATTTTGCACTATGCATGGCATGGGCAGGGGCAGTCTCCATCAATTCGCCAACTTATAAT GCATGCAATGGCAGAATGGTACATGAGAGGGGAGCAGTATGATCAGGCCAAATTGTCTCGAATACTTGAC GTGGCCCAGGACTTGAAGGCCCTGTCGATGCTGCTGAACGGCACTCCATTTGCTTTTGTCATTGACCTTG CTGCACTCGCTTCACGGCGGGAATACCTCAAACTTGACAAGTGGCTAACAGATAAGATAAGAGAGCATGG GGAGCCTTTTATACAAGCATGTATGACTTTTTTAAAGAGAAGGTGCCCTTCTATTTTGGGTGGACTTGCC CCAGAGAAAGATCAGCCCAAAAGTGCTCAACTTCCCGCTGAAACTCTGGCAACAATGTTGGCTTGTCTGC AGGCTTGTGCAGGGAGTGTTTCTCAAGAGTTATCAGAGACCATCCTCACCATGGTAGCCAATTGCAGTAA TGTAATGAATAAGGCCAGACAGCCACCACCTGGAGTTATGCCAAAAGGTCGCCCTCCTAGTGCTAGCAGC TTAGATGCCATTTCTCCTGTTCAGATTGACCCTCTTGCTGGAATGGCATCTCTTAGTATAGGTGGTTCGG CTGCCCCTCACACCCAGAGCATGCAGGGTTTTCCTCCAAATCTGGGTTCTGCGTTCAGTACCCCTCAGTC ACCAGCAAAAGCATTTCCACCACTTTCAACCCCAAATCAAACAACGGCCTTCAGTGGGATTGGAGGACTC TCCTCACAGCTTCCAGTAGGTGGTCTTGGCACAGGAAGCCTGACTGGCATAGGAACTGGGGCCCTTGGAC TTCCTGCAGTGAACAATGATCCTTTTGTACAGAGGAAACTGGGCACCTCTGGACTCAACCAGCCTACATT CCAGCAGACCGACTTGTCTCAGGTGTGGCCAGAGGCAAATCAGCACTTTAGTAAAGAAATTGATGATGAA GCAAACAGTTATTTCCAGCGAATATATAATCACCCACCACATCCAACCATGTCTGTTGATGAGGTATTAG AAATGCTACAGAGATTTAAAGACTCTACAATAAAAAGGGAACGAGAAGTATTTAACTGTATGCTGAGGAA CTTGTTTGAAGAATACCGGTTTTTCCCTCAGTACCCTGATAAAGAATTACATATAACAGCTTGCTTGTTT GGTGGCATAATTGAAAAAGGACTGGTCACTTACATGGCATTGGGTCTGGCCCTGCGATATGTTCTTGAAG CCTTACGGAAGCCTTTTGGATCCAAAATGTATTATTTTGGTATTGCTGCACTAGATAGATTTAAAAATAG ATTGAAGGACTATCCTCAGTATTGTCAGCACCTGGCTTCTATCAGCCACTTTATGCAGTTCCCACATCAT TTGCAGGAGTACATTGAGTACGGACAGCAGTCTCGAGATCCTCCTGTGAAAATGCAGGGCTCTATTACTA CCCCTGGAAGTATTGCACTGGCTCAGGCCCAGGCTCAGGCCCAGGTTCCAGCAAAAGCTCCTCTTGCCGG TCAGGTTAACACAATGGTAACCACCTCAACAACCACCACTGTTGCTAAAACGGTTACAGTCACCAAGCCA ACGGGAGTCAGCTTTAAGAAAGATGTGCCACCTTCTATAAATACAACAAATATCGATACATTGCTTGTGG CCACAGATCAAACTGAGAGGATTGTGGAGCCCCCAGAAAATATTCAAGAGAAAATTGCTTTTATTTTCAA CAATCTCTCCCAGTCAAATATGACACAGAAGGTTGAAGAGCTTAAAGAAACTGTGAAGGAAGAATTTATG CCTTGGGTTTCCCAGTATCTGGTTATGAAAAGAGTCAGTATTGAGCCAAACTTTCATAGCCTGTATTCAA ACTTTCTTGATACATTGAAGAATCCTGAATTTAACAAGATGGTTCTGAATGAAACCTACAGAAACATTAA AGTGCTGCTGACTTCTGATAAAGCTGCGGCCAATTTCTCAGATCGTTCTTTGCTGAAGAACTTGGGACAT TGGTTAGGAATGATCACATTAGCTAAAAATAAACCCATCTTACACACAGACTTGGATGTGAAATCTTTGC TGTTAGAGGCTTACGTTAAAGGACAGCAAGAGTTGCTCTATGTGGTGCCCTTTGTTGCCAAAGTTCTAGA GTCCAGCATTCGCAGCCTGGTTTTTAGACCACCAAACCCCTGGACAATGGCAATTATGAATGTATTGGCT GAGCTGCATCAGGAGCATGACTTAAAGTTAAACCTGAAGTTTGAGATTGAGGTGCTCTGTAAGAACCTTG CATTAGACATCAATGAGCTCAAACCTGGAAACCTGCTGAAAGATAAAGATCGTCTGAAGAACTTAGATGA GCAACTCTCTGCTCCAAAGAAAGATGTCAAGCAGCCCGAAGAGCTTCCTGCCATCACAACAACAACAACT TCTACAACACCAGCTACCAGTACTACGTGTACAGCCACAGTTCCACCACAGCCACAGTACAGCTACCACG ACATCAATGTCTACTCTCTAGCAGGCCTGGCACCTCATATTACTCTAAACCCTACAATCCCTTTATTTCA AGCTCATCCTCAGCTGAAGCAGTGTGTGCGGCAAGCAATTGAAAGAGCTGTCCAGGAGCTTGTCCATCCT GTGGTGGATCGCTCAATTAAGATTGCTATGACTACGTGCGAACAGATAGTCAGAAAGGACTTTGCCCTGG ATTCTGAGGAGTCTCGAATGAGAATAGCAGCTCATCACATGATGCGGAACTTGACAGCTGGAATGGCTAT GATTACTTGCAGGGAACCTTTACTGATGAGCATATCCACCAACCTCAAAAACAGCTTTGCCTCAGCCCTG CGTACTGCATCCCCACAGCAAAGGGAAATGATGGACCAGGCTGCTGCTCAGTTAGCTCAGGACAATTGTG AATTAGCTTGTTGTTTTATTCAGAAAACTGCAGTGGAGAAAGCAGGCCCTGAGATGGACAAGAGATTGGC AACTGAATTTGAGCTGAGGAAGCATGCCCGGCAAGAAGGACGTAGGTACTGTGATCCTGTGGTCTTAACC TACCAAGCCGAGCGGATGCCGGAGCAGATCAGGCTGAAAGTTGGTGGTGTGGACCCAAAGCAGCTAGCCG TCTATGAAGAGTTTGCACGAAATGTGCCTGGCTTCTTACCTACAAATGACTTAAGTCAGCCTACAGGGTT TTTAGCTCAGCCCATGAAGCAAGCTTGGGCAACTGATGATGTAGCGCAGATTTATGATAAGTGTATTACA GAACTGGAGCAGCACCTACACGCCATTCCACCCACTCTGGCCATGAATCCTCAAGCCCAGGCTCTCCGAA GTCTTTTAGAGGTTGTAGTGTTGTCTCGGAATTCTCGGGATGCGATAGCTGCTCTTGGATTACTACAGAA GGCTGTAGAAGGCTTATTAGATGCCACAAGTGGTGCTGACGCCGACTTACTGCTGCGATACAGGGAGTGC CACCTGTTGGTACTGAAAGCTCTGCAGGATGGCAGGGCATATGGATCTCCATGGTGCAACAAACAAATCA CAAGGTGCCTAATTGAATGTCGAGATGAATATAAATATAATGTGGAGGCTGTGGAATTGTTAATTCGAAA TCATTTGGTTAACATGCAGCAGTATGATCTTCATCTTGCTCAGTCGATGGAGAATGGCTTGAACTATATG GCCGTGGCGTTTGCTATGCAACTGGTAAAAATTCTGCTGGTGGATGAAAGGAGTGTTGCCCATATTACTG AGGCAGATCTGTTCCATACCATTGAAACCCTCATGAGAATTAATGCTCATTCCAGAGGCAATGCTCCAGA AGGGTTGCCCCAGCTGATGGAAGTGGTGAGATCCAACTATGAAGCAATGATTGATCGTGCTCATGGAGGC CCTAACTTTATGATGCATTCTGGGATCTCTCAAGCCTCAGAATATGATGACCCTCCAGGCCTGAGGGAGA AGGCAGAGTATCTTCTGAGGGAATGGGTGAATCTCTACCATTCAGCAGCAGCTGGCCGCGACAGTACCAA AGCATTCTCTGCGTTTGTTGGACAGATGCACCAACAAGGAATACTTAAAACTGATGACCTCATAACAAGG TTTTTTCGTCTTTGCACTGAAATGTGTGTTGAAATCAGTTACCGTGCTCAGGCGGAGCAGCAGCACAATC CTGCGGCTAACCCTACCATGATCCGGGCCAAGTGCTACCACAACCTGGATGCCTTTGTCCGCCTCATTGC ACTGTTAGTGAAGCACTCTGGGGAGGCCACCAACACTGTCACAAAGATCAACCTGCTCAATAAGGTTCTC GGTATAGTAGTGGGAGTTCTCCTACAGGATCATGATGTTCGGCAGAGTGAATTTCAGCAACTTCCGTACC ATAGGATTTTTATCATGCTGCTGTTGGAGCTCAATGCACCCGAGCATGTGTTGGAAACCATTAATTTCCA GACTCTTACTGCTTTCTGCAATACATTCCACATCTTGAGACCTACTAAAGCTCCTGGCTTTGTATATGCT TGGCTCGAATTGATTTCCCATCGGATCTTTATTGCAAGAATGCTGGCACATACGCCACAGCAGAAGGGGT GGCCTATGTATGCTCAGCTGTTGATTGATTTATTCAAATATTTAGCACCTTTCCTTAGAAATGTGGAACT CACTAAACCTATGCAAATCCTCTACAAGGGTACTCTGAGAGTGCTGCTGGTTCTTTTGCATGATTTCCCA GAGTTCCTTTGTGATTACCATTATGGGTTCTGTGATGTGATCCCTCCTAATTGTATCCAGTTAAGAAATT TGATCCTGAGTGCCTTCCCAAGAAACATGAGGCTCCCAGACCCATTCACTCCTAATCTGAAGGTGGACAT GTTAAGCGAAATTAACATCGCACCTCGGATCCTCACCAATTTCACTGGAGTGATGCCGCCTCAGTTCAAA AAGGATTTGGATTCTTATCTTAAAACTCGGTCGCCGGTCACTTTCCTGTCTGATCTGCGTAGTAACCTAC AGGTATCTAATGAGCCCGGGAATCGCTACAACCTCCAGCTTATCAATGCATTGGTGCTCTACGTAGGGAC TCAGGCTATCGCACACATCCACAACAAGGGCAGCACGCCCTCAATGAGCACCATCACCCACTCAGCACAC ATGGACATCTTCCAGAACCTGGCTGTGGACCTAGACACTGAGGGCCGATATCTCTTCTTAAATGCAATTG CGAACCAGCTACGGTACCCAAACAGCCACACTCACTACTTCAGCTGCACAATGTTGTACCTTTTTGCTGA GGCGAACACTGAGGCAATCCAGGAACAGATCACAAGGGTTCTCTTGGAACGGTTGATTGTAAATAGGCCA CATCCTTGGGGTCTTCTTATCACCTTCATTGAGCTGATTAAAAACCCAGCATTTAAGTTCTGGAACCATG AATTTGTACACTGTGCCCCAGAGATTGAAAAGTTATTTCAGTCTGTTGCACAGTGCTGCATGGGACAGAA GCAGGCTCAGCAAGTGATGGAGGGGACCGGTGCCAGT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >MR231947 representing NM_001205226 Red=Cloning site Green=Tags(s) MNLDSLSLALSQISYLVDNLTKKNYRASQQEIQHIVNRHGPEADRHLLRCLFSHVDFSGDGKSSGKDFHQ FLIQECASLITKPNFISTLSYAIDNPLHYQKSLKPAPHLFAQLSKVLKLSKVQEVIFGLALLNSSSPDLR GFAAQFIKQKLPDLLRSYIDADVSGNQEGGFQDIAIEVLHLLLSHLLFGQKGAFGVGQEQIDAFLKTLRR DFPQERCPVVLAPLLYPEKRDILMDRILPDSGGVAKTMMESSLADFMQEVGYGFCASIEECRNIIMQFGV REVTAAQVARVLGMMARTHSGLTDGIPLQSISAPGSGIWSDGKDKSEGAQAHTWNVEVLIDVLKELNPSL NFKEVTYELDHPGFQIRDSKGLHNVVYGIQRGLGMEVFPVDFIYRPWKHAEGQLSFIQHSLINPEVFCFA DYPCHTVATDILKAPPEDDNREIATWKSLDLIESLLRLAEVGQYEQVKQLFSFPIKHCPDMLVLALLQIN TSWHTLRHELISTLMPIFLGNHPNSAIILHYAWHGQGQSPSIRQLIMHAMAEWYMRGEQYDQAKLSRILD VAQDLKALSMLLNGTPFAFVIDLAALASRREYLKLDKWLTDKIREHGEPFIQACMTFLKRRCPSILGGLA PEKDQPKSAQLPAETLATMLACLQACAGSVSQELSETILTMVANCSNVMNKARQPPPGVMPKGRPPSASS LDAISPVQIDPLAGMASLSIGGSAAPHTQSMQGFPPNLGSAFSTPQSPAKAFPPLSTPNQTTAFSGIGGL SSQLPVGGLGTGSLTGIGTGALGLPAVNNDPFVQRKLGTSGLNQPTFQQTDLSQVWPEANQHFSKEIDDE ANSYFQRIYNHPPHPTMSVDEVLEMLQRFKDSTIKREREVFNCMLRNLFEEYRFFPQYPDKELHITACLF GGIIEKGLVTYMALGLALRYVLEALRKPFGSKMYYFGIAALDRFKNRLKDYPQYCQHLASISHFMQFPHH LQEYIEYGQQSRDPPVKMQGSITTPGSIALAQAQAQAQVPAKAPLAGQVNTMVTTSTTTTVAKTVTVTKP TGVSFKKDVPPSINTTNIDTLLVATDQTERIVEPPENIQEKIAFIFNNLSQSNMTQKVEELKETVKEEFM PWVSQYLVMKRVSIEPNFHSLYSNFLDTLKNPEFNKMVLNETYRNIKVLLTSDKAAANFSDRSLLKNLGH WLGMITLAKNKPILHTDLDVKSLLLEAYVKGQQELLYVVPFVAKVLESSIRSLVFRPPNPWTMAIMNVLA ELHQEHDLKLNLKFEIEVLCKNLALDINELKPGNLLKDKDRLKNLDEQLSAPKKDVKQPEELPAITTTTT STTPATSTTCTATVPPQPQYSYHDINVYSLAGLAPHITLNPTIPLFQAHPQLKQCVRQAIERAVQELVHP VVDRSIKIAMTTCEQIVRKDFALDSEESRMRIAAHHMMRNLTAGMAMITCREPLLMSISTNLKNSFASAL RTASPQQREMMDQAAAQLAQDNCELACCFIQKTAVEKAGPEMDKRLATEFELRKHARQEGRRYCDPVVLT YQAERMPEQIRLKVGGVDPKQLAVYEEFARNVPGFLPTNDLSQPTGFLAQPMKQAWATDDVAQIYDKCIT ELEQHLHAIPPTLAMNPQAQALRSLLEVVVLSRNSRDAIAALGLLQKAVEGLLDATSGADADLLLRYREC HLLVLKALQDGRAYGSPWCNKQITRCLIECRDEYKYNVEAVELLIRNHLVNMQQYDLHLAQSMENGLNYM AVAFAMQLVKILLVDERSVAHITEADLFHTIETLMRINAHSRGNAPEGLPQLMEVVRSNYEAMIDRAHGG PNFMMHSGISQASEYDDPPGLREKAEYLLREWVNLYHSAAAGRDSTKAFSAFVGQMHQQGILKTDDLITR FFRLCTEMCVEISYRAQAEQQHNPAANPTMIRAKCYHNLDAFVRLIALLVKHSGEATNTVTKINLLNKVL GIVVGVLLQDHDVRQSEFQQLPYHRIFIMLLLELNAPEHVLETINFQTLTAFCNTFHILRPTKAPGFVYA WLELISHRIFIARMLAHTPQQKGWPMYAQLLIDLFKYLAPFLRNVELTKPMQILYKGTLRVLLVLLHDFP EFLCDYHYGFCDVIPPNCIQLRNLILSAFPRNMRLPDPFTPNLKVDMLSEINIAPRILTNFTGVMPPQFK KDLDSYLKTRSPVTFLSDLRSNLQVSNEPGNRYNLQLINALVLYVGTQAIAHIHNKGSTPSMSTITHSAH MDIFQNLAVDLDTEGRYLFLNAIANQLRYPNSHTHYFSCTMLYLFAEANTEAIQEQITRVLLERLIVNRP HPWGLLITFIELIKNPAFKFWNHEFVHCAPEIEKLFQSVAQCCMGQKQAQQVMEGTGAS TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001205226
ORF Size	7107 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001205226.2
RefSeq Size	8369 bp
RefSeq ORF	7110 bp
Locus ID	234594
MW	266.6 kDa
Gene Summary	Scaffolding component of the CCR4-NOT complex which is one of the major cellular mRNA deadenylases and is linked to various cellular processes including bulk mRNA degradation, miRNA-mediated repression, translational repression during translational initiation and general transcription regulation. Additional complex functions may be a consequence of its influence on mRNA expression. Its scaffolding function implies its interaction with the catalytic complex module and diverse RNA-binding proteins mediating the complex recruitment to selected mRNA 3' UTRs. Involved in degradation of AU-rich element (ARE)-containing mRNAs probably via association with ZFP36. Mediates the recruitment of the CCR4-NOT complex to miRNA targets and to the RISC complex via association with TNRC6A, TNRC6B or TNRC6C. Acts as a transcriptional repressor. Represses the ligand-dependent transcriptional activation by nuclear receptors. Involved in the maintenance of embryonic stem (ES) cell identity; prevents their differentiation towards extraembryonic trophectoderm lineages.[UniProtKB/Swiss-Prot Function]

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