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p53 (TP53) (NM_000546) Human Tagged ORF Clone
CAT＃: RC200003
TP53 (Myc-DDK-tagged)-Human tumor protein p53 (TP53), transcript variant 1
ORF Plasmid: tGFP
"NM_000546" in other vectors (6)
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CNY 3,656.00
CNY 5,320.00
| Cited in 23 publications. |
CNY 300.00
CNY 1,999.00
CNY 2,700.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | BCC7; BMFS5; LFS1; P53; TRP53 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC200003 representing NM_000546.
Blue=ORF Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGGAGGAGCCGCAGTCAGATCCTAGCGTCGAGCCCCCTCTGAGTCAGGAAACATTTTCAGACCTATGG AAACTACTTCCTGAAAACAACGTTCTGTCCCCCTTGCCGTCCCAAGCAATGGATGATTTGATGCTGTCC CCGGACGATATTGAACAATGGTTCACTGAAGACCCAGGTCCAGATGAAGCTCCCAGAATGCCAGAGGCT GCTCCCCCCGTGGCCCCTGCACCAGCAGCTCCTACACCGGCGGCCCCTGCACCAGCCCCCTCCTGGCCC CTGTCATCTTCTGTCCCTTCCCAGAAAACCTACCAGGGCAGCTACGGTTTCCGTCTGGGCTTCTTGCAT TCTGGGACAGCCAAGTCTGTGACTTGCACGTACTCCCCTGCCCTCAACAAGATGTTTTGCCAACTGGCC AAGACCTGCCCTGTGCAGCTGTGGGTTGATTCCACACCCCCGCCCGGCACCCGCGTCCGCGCCATGGCC ATCTACAAGCAGTCACAGCACATGACGGAGGTTGTGAGGCGCTGCCCCCACCATGAGCGCTGCTCAGAT AGCGATGGTCTGGCCCCTCCTCAGCATCTTATCCGAGTGGAAGGAAATTTGCGTGTGGAGTATTTGGAT GACAGAAACACTTTTCGACATAGTGTGGTGGTGCCCTATGAGCCGCCTGAGGTTGGCTCTGACTGTACC ACCATCCACTACAACTACATGTGTAACAGTTCCTGCATGGGCGGCATGAACCGGAGGCCCATCCTCACC ATCATCACACTGGAAGACTCCAGTGGTAATCTACTGGGACGGAACAGCTTTGAGGTGCGTGTTTGTGCC TGTCCTGGGAGAGACCGGCGCACAGAGGAAGAGAATCTCCGCAAGAAAGGGGAGCCTCACCACGAGCTG CCCCCAGGGAGCACTAAGCGAGCACTGCCCAACAACACCAGCTCCTCTCCCCAGCCAAAGAAGAAACCA CTGGATGGAGAATATTTCACCCTTCAGATCCGTGGGCGTGAGCGCTTCGAGATGTTCCGAGAGCTGAAT GAGGCCTTGGAACTCAAGGATGCCCAGGCTGGGAAGGAGCCAGGGGGGAGCAGGGCTCACTCCAGCCAC CTGAAGTCCAAAAAGGGTCAGTCTACCTCCCGCCATAAAAAACTCATGTTCAAGACAGAAGGGCCTGAC TCAGAC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC >Peptide sequence encoded by RC200003
Blue=ORF Red=Cloning site Green=Tag(s) MEEPQSDPSVEPPLSQETFSDLWKLLPENNVLSPLPSQAMDDLMLSPDDIEQWFTEDPGPDEAPRMPEA APPVAPAPAAPTPAAPAPAPSWPLSSSVPSQKTYQGSYGFRLGFLHSGTAKSVTCTYSPALNKMFCQLA KTCPVQLWVDSTPPPGTRVRAMAIYKQSQHMTEVVRRCPHHERCSDSDGLAPPQHLIRVEGNLRVEYLD DRNTFRHSVVVPYEPPEVGSDCTTIHYNYMCNSSCMGGMNRRPILTIITLEDSSGNLLGRNSFEVRVCA CPGRDRRTEEENLRKKGEPHHELPPGSTKRALPNNTSSSPQPKKKPLDGEYFTLQIRGRERFEMFRELN EALELKDAQAGKEPGGSRAHSSHLKSKKGQSTSRHKKLMFKTEGPDSD TRTRPLEQKLISEEDLAANDILDYKDDDDKV Recombinant protein using RC200003 also available, TP300003 |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_000546 |
| ORF Size | 1179 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_000546.6 |
| RefSeq Size | 2591 bp |
| RefSeq ORF | 1182 bp |
| Locus ID | 7157 |
| UniProt ID | P04637 |
| Domains | P53 |
| Protein Families | Druggable Genome, Stem cell - Pluripotency, Transcription Factors |
| Protein Pathways | Amyotrophic lateral sclerosis (ALS), Apoptosis, Basal cell carcinoma, Bladder cancer, Cell cycle, Chronic myeloid leukemia, Colorectal cancer, Endometrial cancer, Glioma, Huntington's disease, MAPK signaling pathway, Melanoma, Neurotrophin signaling pathway, Non-small cell lung cancer, p53 signaling pathway, Pancreatic cancer, Pathways in cancer, Prostate cancer, Small cell lung cancer, Thyroid cancer, Wnt signaling pathway |
| MW | 43.7 kDa |
| Gene Summary | This gene encodes a tumor suppressor protein containing transcriptional activation, DNA binding, and oligomerization domains. The encoded protein responds to diverse cellular stresses to regulate expression of target genes, thereby inducing cell cycle arrest, apoptosis, senescence, DNA repair, or changes in metabolism. Mutations in this gene are associated with a variety of human cancers, including hereditary cancers such as Li-Fraumeni syndrome. Alternative splicing of this gene and the use of alternate promoters result in multiple transcript variants and isoforms. Additional isoforms have also been shown to result from the use of alternate translation initiation codons from identical transcript variants (PMIDs: 12032546, 20937277). [provided by RefSeq, Dec 2016] |
Citations (23)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
A Proteogenomic Atlas of Clear Cell Renal Cell Carcinoma in a Chinese Population
,Ding, C;Qu, Y;Feng, J;Wu, X;Bai, L;Xu, W;Zhu, L;Liu, Y;Xu, F;Zhang, X;Yang, G;Lv, J;Chen, X;Shi, G;Wang, H;Cao, D;Xiang, H;Li, L;Tan, S;Gan, H;Sun, M;Zhang, H;Zhao, J;Ye, D;,
Research Square
[p53]
|
|
AXL Inactivation Inhibits Mesothelioma Growth and Migration via Regulation of p53 Expression
,Song, W;Wang, H;Lu, M;Ni, X;Bahri, N;Zhu, S;Chen, L;Wu, Y;Qiu, J;Fletcher, JA;Ou, WB;,
Cancers
,PubMed ID 32992696
[p53]
|
|
The endoplasmic reticulum acetyltransferases ATase1/NAT8B and ATase2/NAT8 are differentially regulated to adjust engagement of the secretory pathway
,null,
Journal of neurochemistry
,PubMed ID 31945187
[p53]
|
|
High-resolution protein–protein interaction mapping using all-versus-all sequencing (AVA-Seq)
,null,
The Journal of Biological Chemistry
,PubMed ID 31182485
[p53]
|
|
Inhibition of DYRK1A-EGFR axis by p53-MDM2 cascade mediates the induction of cellular senescence
,Xu, X;Liu, Q;Zhang, C;Ren, S;Xu, L;Zhao, Z;Dou, H;Li, P;Zhang, X;Gong, Y;Shao, C;,
Cell Death Dis
,PubMed ID 30910997
[p53]
|
|
DRAM2 acts as an oncogene in non-small cell lung cancer and suppresses the expression of p53
,Wudu, M;Ren, H;Hui, L;Jiang, J;Zhang, S;Xu, Y;Wang, Q;Su, H;Jiang, X;Dao, R;Qiu, X;,
J. Exp. Clin. Cancer Res.
,PubMed ID 30755245
[p53]
|
|
Oncogenic KSHV-encoded interferon regulatory factor upregulates HMGB2 and CMPK1 expression to promote cell invasion by disrupting a complex lncRNA-OIP5-AS1/miR-218-5p network
,Li, W;Wang, Q;Feng, Q;Wang, F;Yan, Q;Gao, SJ;Lu, C;,
PLoS Pathog.
,PubMed ID 30699189
[p53]
|
|
Identification and characterization of synthetic viability with ERCC1 deficiency in response to interstrand crosslinks in lung cancer
,Heyza, J;Lei, W;Watza, D;Zhang, H;Chen, W;Back, JB;Schwartz, AG;Bepler, G;Patrick, SM;,
Clin. Cancer Res.
,PubMed ID 30538112
[p53]
|
|
Zinc deficiency causes neural tube defects through attenuation of p53 ubiquitylation
,null,
Development (Cambridge, England)
,PubMed ID 30545932
[p53]
|
|
Inhibition of the formation of autophagosome but not autolysosome augments ABT-751-induced apoptosis in TP53-deficient Hep-3B cells
,Wei, RJ;Wu, WR;Pan, CT;Yu, CY;Li, CF;Chen, LR;Liang, SS;Shiue, YL;,
J. Cell. Physiol.
,PubMed ID 30367486
[p53]
|
|
Trans-chalcone increases p53 activity via DNAJB1/HSP40 induction and CRM1 inhibition
,Silva, G;Marins, M;Chaichanasak, N;Yoon, Y;Fachin, AL;Pinhanelli, VC;Regasini, LO;Dos Santos, MB;Ayusso, GM;Marques, BC;Wu, WW;Phue, JN;Shen, RF;Baek, SJ;,
PLoS ONE
,PubMed ID 30118500
[p53]
|
|
CD44 variant isoform 9 emerges in response to injury and contributes to the regeneration of the gastric epithelium
,Bertaux-Skeirik, N;Wunderlich, M;Teal, E;Chakrabarti, J;Biesiada, J;Mahe, M;Sundaram, N;Gabre, J;Hawkins, J;Jian, G;Engevik, AC;Yang, L;Wang, J;Goldenring, JR;Qualls, JE;Medvedovic, M;Helmrath, MA;Diwan, T;Mulloy, JC;Zavros, Y;,
J. Pathol.
,PubMed ID 28497484
[p53]
|
|
iASPP induces EMT and cisplatin resistance in human cervical cancer through miR-20a-FBXL5/BTG3 signaling
,Xiong, Y;Sun, F;Dong, P;Watari, H;Yue, J;Yu, MF;Lan, CY;Wang, Y;Ma, ZB;,
J. Exp. Clin. Cancer Res.
,PubMed ID 28399926
[p53]
|
|
Role of a p53 polymorphism in the development of nonfunctional pituitary adenomas
,Yagnik, G;Jahangiri, A;Chen, R;Wagner, JR;Aghi, MK;,
Mol. Cell. Endocrinol
,PubMed ID 28214592
[p53]
|
|
mir-660-p53-mir-486 Network: A New Key Regulatory Pathway in Lung Tumorigenesis
,Borzi, C;Calzolari, L;Centonze, G;Milione, M;Sozzi, G;Fortunato, O;,
Int J Mol Sci
,PubMed ID 28124991
[p53]
|
|
Suppression of iASPP-dependent aggressiveness in cervical cancer through reversal of methylation silencing of microRNA-124
,Dong, P;Xiong, Y;Watari, H;Hanley, SJ;Konno, Y;Ihira, K;Suzuki, F;Yamada, T;Kudo, M;Yue, J;Sakuragi, N;,
Sci Rep
,PubMed ID 27765948
[p53]
|
|
Influence of Human p53 on Plant Development
,Ma, H;Song, T;Wang, T;Wang, S;,
PLoS ONE
,PubMed ID 27648563
[p53]
|
|
A microtubule inhibitor, ABT-751, induces autophagy and delays apoptosis in Huh-7 cells.
,null,
Toxicology and applied pharmacology
,PubMed ID 27678524
[p53]
|
|
The miR-644a/CTBP1/p53 axis suppresses drug resistance by simultaneous inhibition of cell survival and epithelial-mesenchymal transition in breast cancer
,Raza, U;Saatci, Ö;Uhlmann, S;Ansari, SA;Eyüpoğlu, E;Yurdusev, E;Mutlu, M;Ersan, PG;Altundağ, MK;Zhang, JD;Doğan, HT;Güler, G;Şahin, Ö;,
Oncotarget
,PubMed ID 27409664
[p53]
|
|
Synergy between histone deacetylase inhibitors and DNA-damaging agents is mediated by histone deacetylase 2 in colorectal cancer
,Alzoubi, S;Brody, L;Rahman, S;Mahul-Mellier, AL;Mercado, N;Ito, K;El-Bahrawy, M;Silver, A;Boobis, A;Bell, JD;Hajji, N;,
Oncotarget
,PubMed ID 27283986
[p53]
|
|
The Role and Regulation of Mitochondrial Dynamics in Cisplatin Resistance in Human Gynecologic Cancer Cells
,Kong, B;,
Thesis
[p53]
|
|
p53 is required for cisplatin-induced processing of the mitochondrial fusion protein L-Opa1 that is mediated by the mitochondrial metallopeptidase Oma1 in Gynecologic Cancers
,Kong, B;Wang, Q;Fung, E;Xue, K;Tsang, BK;,
J. Biol. Chem.
,PubMed ID 25112877
[p53]
|
|
Tumor Suppressor Protein p53 Negatively Regulates Human Pregnane X Receptor Activity
,Ayesha Elias, Jing Wu, and Taosheng Chen,
Mol. Pharmacol., Jun 2013; 83: 1229 - 1236.
,PubMed ID 23536728
[p53]
|
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC200003L1 | Lenti ORF clone of Human tumor protein p53 (TP53), transcript variant 1, Myc-DDK-tagged |
CNY 6,056.00 |
|
| RC200003L2 | Lenti ORF clone of Human tumor protein p53 (TP53), transcript variant 1, mGFP tagged |
CNY 6,056.00 |
|
| RC200003L3 | Lenti ORF clone of Human tumor protein p53 (TP53), transcript variant 1, Myc-DDK-tagged |
CNY 6,056.00 |
|
| RC200003L4 | Lenti ORF clone of Human tumor protein p53 (TP53), transcript variant 1, mGFP tagged |
CNY 6,056.00 |
|
| RG200003 | TP53 (tGFP-tagged) - Human tumor protein p53 (TP53), transcript variant 1 |
CNY 5,256.00 |
|
| SC119832 | TP53 (untagged)-Human tumor protein p53 (TP53), transcript variant 1 |
CNY 5,488.00 |
