DCXR (NM_016286) Human Tagged ORF Clone

CAT#: RC200023

DCXR (Myc-DDK-tagged)-Human dicarbonyl/L-xylulose reductase (DCXR), transcript variant 1

ORF Plasmid: DDK tGFP



  "NM_016286" in other vectors (6)

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CNY 2,400.00

CNY 3,705.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (5)
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

CNY 5,420.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00


DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 30 ul

CNY 300.00


Anti-DCXR mouse monoclonal antibody, clone OTI4D11 (formerly 4D11)
    • 100 ul

CNY 1,999.00
CNY 2,700.00

Specifications

Product Data
Type Human Tagged ORF Clone
Tag Myc-DDK
Synonyms DCR; HCR2; HCRII; KIDCR; P34H; PNTSU; SDR20C1; XR
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RC200023 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGAGCTGTTCCTCGCGGGCCGCCGGGTGCTGGTCACCGGGGCAGGCAAAGGTATAGGGCGCGGCACGG
TCCAGGCGCTGCACGCGACGGGCGCGCGGGTGGTGGCTGTGAGCCGGACTCAGGCGGATCTTGACAGCCT
TGTCCGCGAGTGCCCGGGGATAGAACCCGTGTGCGTGGACCTGGGTGACTGGGAGGCCACCGAGCGGGCG
CTGGGCAGCGTGGGCCCCGTGGACCTGCTGGTGAACAACGCCGCTGTCGCCCTGCTGCAGCCCTTCCTGG
AGGTCACCAAGGAGGCCTTTGACAGATCCTTTGAGGTGAACCTGCGTGCGGTCATCCAGGTGTCGCAGAT
TGTGGCCAGGGGCTTAATAGCCCGGGGAGTCCCAGGGGCCATCGTGAATGTCTCCAGCCAGTGCTCCCAG
CGGGCAGTAACTAACCATAGCGTCTACTGCTCCACCAAGGGTGCCCTGGACATGCTGACCAAGGTGATGG
CCCTAGAGCTCGGGCCCCACAAGATCCGAGTGAATGCAGTAAACCCCACAGTGGTGATGACGTCCATGGG
CCAGGCCACCTGGAGTGACCCCCACAAGGCCAAGACTATGCTGAACCGAATCCCACTTGGCAAGTTTGCT
GAGGTAGAGCACGTGGTGAACGCCATCCTCTTTCTGCTGAGTGACCGAAGTGGCATGACCACGGGTTCCA
CTTTGCCGGTGGAAGGGGGCTTCTGGGCCTGC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RC200023 protein sequence
Red=Cloning site Green=Tags(s)

MELFLAGRRVLVTGAGKGIGRGTVQALHATGARVVAVSRTQADLDSLVRECPGIEPVCVDLGDWEATERA
LGSVGPVDLLVNNAAVALLQPFLEVTKEAFDRSFEVNLRAVIQVSQIVARGLIARGVPGAIVNVSSQCSQ
RAVTNHSVYCSTKGALDMLTKVMALELGPHKIRVNAVNPTVVMTSMGQATWSDPHKAKTMLNRIPLGKFA
EVEHVVNAILFLLSDRSGMTTGSTLPVEGGFWAC

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_016286
ORF Size 732 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_016286.4
RefSeq Size 860 bp
RefSeq ORF 735 bp
Locus ID 51181
UniProt ID Q7Z4W1
Domains adh_short
Protein Families Druggable Genome
Protein Pathways Metabolic pathways, Pentose and glucuronate interconversions
MW 25.9 kDa
Gene Summary The protein encoded by this gene acts as a homotetramer to catalyze diacetyl reductase and L-xylulose reductase reactions. The encoded protein may play a role in the uronate cycle of glucose metabolism and in the cellular osmoregulation in the proximal renal tubules. Defects in this gene are a cause of pentosuria. Two transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Aug 2010]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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