EIF2AK1 (NM_014413) Human Tagged ORF Clone
CAT#: RC200065
- TrueORF®
EIF2AK1 (Myc-DDK-tagged)-Human eukaryotic translation initiation factor 2-alpha kinase 1 (EIF2AK1), transcript variant 1
ORF Plasmid: tGFP
"NM_014413" in other vectors (7)
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CNY 4,688.00
CNY 6,270.00
CNY 300.00
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | HCR; hHRI; HRI; LEMSPAD |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RC200065 representing NM_014413
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCAGGGGGGCAACTCCGGGGTCCGCAAGCGCGAAGAGGAGGGCGACGGGGCTGGGGCTGTGGCTGCGC CGCCGGCCATCGACTTTCCCGCCGAGGGCCCGGACCCCGAATATGACGAATCTGATGTTCCAGCAGAAAT CCAGGTGTTAAAAGAACCCCTACAACAGCCAACCTTCCCTTTTGCAGTTGCAAACCAACTCTTGCTGGTT TCTTTGCTGGAGCACTTGAGCCACGTGCATGAACCAAACCCACTTCGTTCAAGACAGGTGTTTAAGCTAC TTTGCCAGACGTTTATCAAAATGGGGCTGCTGTCTTCTTTCACTTGTAGTGACGAGTTTAGCTCATTGAG ACTACATCACAACAGAGCTATTACTCACTTAATGAGGTCTGCTAAAGAGAGAGTTCGTCAGGATCCTTGT GAGGATATTTCTCGTATCCAGAAAATCAGATCAAGGGAAGTAGCCTTGGAAGCACAAACTTCACGTTACT TAAATGAATTTGAAGAACTTGCCATCTTAGGAAAAGGTGGATACGGAAGAGTATACAAGGTCAGGAATAA ATTAGATGGTCAGTATTATGCAATAAAAAAAATCCTGATTAAGGGTGCAACTAAAACAGTTTGCATGAAG GTCCTACGGGAAGTGAAGGTGCTGGCAGGTCTTCAGCACCCCAATATTGTTGGCTATCACACCGCGTGGA TAGAACATGTTCATGTGATTCAGCCACGAGCAGACAGAGCTGCCATTGAGTTGCCATCTCTGGAAGTGCT CTCCGACCAGGAAGAGGACAGAGAGCAATGTGGTGTTAAAAATGATGAAAGTAGCAGCTCATCCATTATC TTTGCTGAGCCCACCCCAGAAAAAGAAAAACGCTTTGGAGAATCTGACACTGAAAATCAGAATAACAAGT CGGTGAAGTACACCACCAATTTAGTCATAAGAGAATCTGGTGAACTTGAGTCGACCCTGGAGCTCCAGGA AAATGGCTTGGCTGGTTTGTCTGCCAGTTCAATTGTGGAACAGCAGCTGCCACTCAGGCGTAATTCCCAC CTAGAGGAGAGTTTCACATCCACCGAAGAATCTTCCGAAGAAAATGTCAACTTTTTGGGTCAGACAGAGG CACAGTACCACCTGATGCTGCACATCCAGATGCAGCTGTGTGAGCTCTCGCTGTGGGATTGGATAGTCGA GAGAAACAAGCGGGGCCGGGAGTATGTGGACGAGTCTGCCTGTCCTTATGTTATGGCCAATGTTGCAACA AAAATTTTTCAAGAATTGGTAGAAGGTGTGTTTTACATACATAACATGGGAATTGTGCACCGAGATCTGA AGCCAAGAAATATTTTTCTTCATGGCCCTGATCAGCAAGTAAAAATAGGAGACTTTGGTCTGGCCTGCAC AGACATCCTACAGAAGAACACAGACTGGACCAACAGAAACGGGAAGAGAACACCAACACATACGTCCAGA GTGGGTACTTGTCTGTACGCTTCACCCGAACAGTTGGAAGGATCTGAGTATGATGCCAAGTCAGATATGT ACAGCTTGGGTGTGGTCCTGCTAGAGCTCTTTCAGCCGTTTGGAACAGAAATGGAGCGAGCAGAAGTTCT AACAGGTTTAAGAACTGGTCAGTTGCCGGAATCCCTCCGTAAAAGGTGTCCAGTGCAAGCCAAGTATATC CAGCACTTAACGAGAAGGAACTCATCGCAGAGACCATCTGCCATTCAGCTGCTGCAGAGTGAACTTTTCC AAAATTCTGGAAATGTTAACCTCACCCTACAGATGAAGATAATAGAGCAAGAAAAAGAAATTGCAGAACT AAAGAAGCAGCTAAACCTCCTTTCTCAAGACAAAGGGGTGAGGGATGACGGAAAGGATGGGGGCGTGGGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC200065 representing NM_014413
Red=Cloning site Green=Tags(s) MQGGNSGVRKREEEGDGAGAVAAPPAIDFPAEGPDPEYDESDVPAEIQVLKEPLQQPTFPFAVANQLLLV SLLEHLSHVHEPNPLRSRQVFKLLCQTFIKMGLLSSFTCSDEFSSLRLHHNRAITHLMRSAKERVRQDPC EDISRIQKIRSREVALEAQTSRYLNEFEELAILGKGGYGRVYKVRNKLDGQYYAIKKILIKGATKTVCMK VLREVKVLAGLQHPNIVGYHTAWIEHVHVIQPRADRAAIELPSLEVLSDQEEDREQCGVKNDESSSSSII FAEPTPEKEKRFGESDTENQNNKSVKYTTNLVIRESGELESTLELQENGLAGLSASSIVEQQLPLRRNSH LEESFTSTEESSEENVNFLGQTEAQYHLMLHIQMQLCELSLWDWIVERNKRGREYVDESACPYVMANVAT KIFQELVEGVFYIHNMGIVHRDLKPRNIFLHGPDQQVKIGDFGLACTDILQKNTDWTNRNGKRTPTHTSR VGTCLYASPEQLEGSEYDAKSDMYSLGVVLLELFQPFGTEMERAEVLTGLRTGQLPESLRKRCPVQAKYI QHLTRRNSSQRPSAIQLLQSELFQNSGNVNLTLQMKIIEQEKEIAELKKQLNLLSQDKGVRDDGKDGGVG TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
![]() Sequencher program is needed, download here. |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_014413 |
ORF Size | 1890 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_014413.4 |
RefSeq Size | 4465 bp |
RefSeq ORF | 1893 bp |
Locus ID | 27102 |
UniProt ID | Q9BQI3 |
Domains | pkinase, TyrKc, S_TKc |
Protein Families | Druggable Genome, Protein Kinase |
MW | 71.1 kDa |
Gene Summary | The protein encoded by this gene acts at the level of translation initiation to downregulate protein synthesis in response to stress. The encoded protein is a kinase that can be inactivated by hemin. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2008] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC200065L1 | Lenti ORF clone of Human eukaryotic translation initiation factor 2-alpha kinase 1 (EIF2AK1), transcript variant 1, Myc-DDK-tagged |
CNY 8,170.00 |
|
RC200065L2 | Lenti ORF clone of Human eukaryotic translation initiation factor 2-alpha kinase 1 (EIF2AK1), transcript variant 1, mGFP tagged |
CNY 7,088.00 |
|
RC200065L3 | Lenti ORF clone of Human eukaryotic translation initiation factor 2-alpha kinase 1 (EIF2AK1), transcript variant 1, Myc-DDK-tagged |
CNY 8,170.00 |
|
RC200065L4 | Lenti ORF clone of Human eukaryotic translation initiation factor 2-alpha kinase 1 (EIF2AK1), transcript variant 1, mGFP tagged |
CNY 8,170.00 |
|
RG200065 | EIF2AK1 (tGFP-tagged) - Human eukaryotic translation initiation factor 2-alpha kinase 1 (EIF2AK1), transcript variant 1 |
CNY 6,288.00 |
|
SC115013 | EIF2AK1 (untagged)-Human eukaryotic translation initiation factor 2-alpha kinase 1 (EIF2AK1), transcript variant 1 |
CNY 4,696.00 |
|
SC323477 | EIF2AK1 (untagged)-Kinase deficient mutant (K210M) of Human eukaryotic translation initiation factor 2-alpha kinase 1 (EIF2AK1), transcript variant 1 |
CNY 4,696.00 |
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