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ENTR1 (NM_006643) Human Tagged ORF Clone
CAT＃: RC200139
SDCCAG3 (Myc-DDK-tagged)-Human serologically defined colon cancer antigen 3 (SDCCAG3), transcript variant 2
ORF Plasmid: tGFP
"NM_006643" in other vectors (7)
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CNY 3,656.00
CNY 5,320.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | NY-CO-3; SDCCAG3; SDDAG3 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC200139 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCGGGCTACCAGCGCCACCCGGGCGCCACCCCGCTGTCCCGAGCCCGGAGCCTCGCCATTCCCGACG CTCCAGCGTTCTATGAGCGCCGGTCTTGTCTCCCCCAGCTAAATTGTGAGCGCCCCCATGGCAGGGACCT GGACTCCCCCTTCTTCGGCATTCGGCCGGCCTTTATGTGCTATGTGCCCAGCCCGGTGCTGGCTTCCGTG GGAGACACAGATGACAGATTTGAAGATCTGGAAGAGGCAAATCCATTCTCTTTTAGAGAGTTTCTGAAGA CCAAGAACCTCGGCCTCTCGAAAGAGGATCCGGCCAGCAGAATTTATGCAAAGGAAGCCTCGAGGCATTC CCTGGGACTTGACCACAACTCCCCACCCTCCCAAACCGGCGGGTATGGCCTGGAGTATCAGCAGCCATTT TTCGAGGATCCGACAGGGGCTGGTGACCTCCTGGATGGGGAGGAGGATGAGGACACCGGATGGAGTGGGG CCTACCTGCCGTCCGCCATCGAGCAGACTCACCCCGAGAGGGTCCCTGCCGGCACGTCGCCCTGCAGCAC ATACCTTTCCTTTTTCTCCACCCCGTCGGAGCTGGCAGGGCCTGAGTCTCTGCCCTCGTGGGCGTTGAGT GACACTGATTCTCGCGTGTCTCCGGCCTCTCCGGCAGGGAGTCCTAGCGCAGACTTTGCGGTTCATGGAG AGTCTCTGGGAGACAGGCACCTGCGGACGCTGCAGATAAGTTACGACGCACTGAAAGATGAAAATTCTAA GCTGAGAAGAAAGCTGAATGAGGTTCAGAGCTTCTCTGAAGCTCAAACAGAAATGGTGAGGACGCTTGAG CGGAAGTTAGAAGCAAAAATGATCAAGGAGGAAAGCGACTACCACGACCTGGAGTCGGTGGTTCAGCAGG TGGAGCAGAACCTGGAGCTGATGACCAAACGGGCTGTAAAGGCAGAAAACCACGTCGTGAAACTAAAACA GGAAATCAGTTTGCTCCAGGCGCAGGTCTCCAACTTCCAGCGAGAGAATGAAGCCCTGCGGTGCGGCCAG GGCGCCAGCCTGACCGTGGTGAAGCAGAACGCCGACGTGGCCCTGCAGAACCTCCGGGTGGTCATGAACA GTGCACAGGCTTCCATCAAGCAACTGGTTTCCGGAGCTGAGACACTGAATCTTGTTGCCGAAATCCTTAA ATCTATAGACAGAATTTCTGAAATTAAAGACGAGGAGGAAGACTCT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC200139 protein sequence
Red=Cloning site Green=Tags(s) MSGYQRHPGATPLSRARSLAIPDAPAFYERRSCLPQLNCERPHGRDLDSPFFGIRPAFMCYVPSPVLASV GDTDDRFEDLEEANPFSFREFLKTKNLGLSKEDPASRIYAKEASRHSLGLDHNSPPSQTGGYGLEYQQPF FEDPTGAGDLLDGEEDEDTGWSGAYLPSAIEQTHPERVPAGTSPCSTYLSFFSTPSELAGPESLPSWALS DTDSRVSPASPAGSPSADFAVHGESLGDRHLRTLQISYDALKDENSKLRRKLNEVQSFSEAQTEMVRTLE RKLEAKMIKEESDYHDLESVVQQVEQNLELMTKRAVKAENHVVKLKQEISLLQAQVSNFQRENEALRCGQ GASLTVVKQNADVALQNLRVVMNSAQASIKQLVSGAETLNLVAEILKSIDRISEIKDEEEDS TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_006643 |
| ORF Size | 1236 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_006643.3, NP_006634.3 |
| RefSeq Size | 2321 bp |
| RefSeq ORF | 1239 bp |
| Locus ID | 10807 |
| UniProt ID | Q96C92 |
| MW | 45.5 kDa |
| Gene Summary | Endosome-associated protein that plays a role in membrane receptor sorting, cytokinesis and ciliogenesis (PubMed:23108400, PubMed:25278552, PubMed:27767179). Involved in the endosome-to-plasma membrane trafficking and recycling of SNX27-retromer-dependent cargo proteins, such as GLUT1 (PubMed:25278552). Involved in the regulation of cytokinesis; the function may involve PTPN13 and GIT1 (PubMed:23108400). Plays a role in the formation of cilia (PubMed:27767179). Involved in cargo protein localization, such as PKD2, at primary cilia (PubMed:27767179). Involved in the presentation of the tumor necrosis factor (TNF) receptor TNFRSF1A on the cell surface, and hence in the modulation of the TNF-induced apoptosis (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC200139L1 | Lenti ORF clone of Human serologically defined colon cancer antigen 3 (SDCCAG3), transcript variant 2, Myc-DDK-tagged |
CNY 6,056.00 |
|
| RC200139L2 | Lenti ORF clone of Human serologically defined colon cancer antigen 3 (SDCCAG3), transcript variant 2, mGFP tagged |
CNY 5,890.00 |
|
| RC200139L3 | Lenti ORF clone of Human serologically defined colon cancer antigen 3 (SDCCAG3), transcript variant 2, Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC200139L4 | Lenti ORF clone of Human serologically defined colon cancer antigen 3 (SDCCAG3), transcript variant 2, mGFP tagged |
CNY 5,890.00 |
|
| RG200139 | SDCCAG3 (tGFP-tagged) - Human serologically defined colon cancer antigen 3 (SDCCAG3), transcript variant 2 |
CNY 4,370.00 |
|
| SC115930 | SDCCAG3 (untagged)-Human serologically defined colon cancer antigen 3 (SDCCAG3), transcript variant 2 |
CNY 3,656.00 |
|
| SC321220 | SDCCAG3 (untagged)-Human serologically defined colon cancer antigen 3 (SDCCAG3), transcript variant 2 |
CNY 3,656.00 |
