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NSUN5 (NM_148956) Human Tagged ORF Clone
CAT＃: RC200144
NSUN5 (Myc-DDK-tagged)-Human NOP2/Sun domain family, member 5 (NSUN5), transcript variant 1
ORF Plasmid: tGFP
"NM_148956" in other vectors (6)
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CNY 3,656.00
CNY 5,320.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | NOL1; NOL1R; NSUN5A; p120; p120(NOL1); WBSCR20; WBSCR20A |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC200144 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGGCTGTATGCTGCAGCTGCAGGCGTGTTGGCCGGCGTGGAGAGCCGCCAGGGCTCTATCAAGGGGT TGGTGTACTCCAGCAACTTCCAGAACGTGAAGCAGCTGTACGCGCTGGTGTGCGAAACGCAGCGCTACTC CGCCGTGCTGGATGCTGTGATCGCCAGCGCCGGCCTCCTCCGTGCGGAGAAGAAGCTGCGGCCGCACCTG GCCAAGGTGCTAGTGTATGAGTTGTTGTTGGGAAAGGGCTTTCGAGGGGGTGGGGGCCGATGGAAGGCTC TGTTGGGCCGGCACCAGGCGAGGCTCAAGGCTGAGTTGGCTCGGCTCAAGGTTCATCGGGGTGTGAGCCG GAATGAGGACCTGTTGGAAGTGGGATCCAGGCCTGGTCCAGCCTCCCAGCTGCCTCGATTTGTGCGTGTG AACACTCTCAAGACCTGCTCCGATGATGTAGTTGATTATTTCAAGAGACAAGGTTTCTCCTATCAGGGTC GGGCTTCCAGCCTCGATGACTTACGAGCCCTCAAGGGGAAGCATTTTCTCCTGGACCCCTTGATGCCGGA GCTGCTGGTGTTTCCCGCCCAGACAGATCTGCATGAACACCCACTGTACCGGGCCGGACACCTCATTCTG CAGGACAGGGCCAGCTGTCTCCCAGCCATGCTGCTGGACCCCCCGCCAGGCTCCCATGTCATCGATGCCT GTGCCGCCCCAGGCAATAAGACCAGTCACTTGGCTGCTCTTCTGAAGAACCAAGGGAAGATCTTTGCCTT TGACCTGGATGCCAAGCGGCTGGCATCCATGGCCACGCTGCTGGCCCGGGCTGGCGTCTCTTGCTGTGAA CTGGCTGAGGAGGACTTCCTGGCGGTCTCCCCCTCGGATCCACGCTACCATGAGGTCCACTACATCCTGC TGGATCCTTCCTGCAGTGGCTCGGGTATGCCGAGCAGACAGCTGGAGGAGCCCGGGGCAGGCACACCTAG CCCGGTGCGTCTGCATGCCCTGGCAGGGTTCCAGCAGCGAGCCCTGTGCCACGCACTCACTTTCCCTTCC CTGCAGCGGCTCGTCTACTCCACGTGCTCCCTCTGCCAGGAGGAGAATGAAGACGTGGTGCGAGATGCGC TGCAGCAGAACCCGGGCGCCTTCAGGCTAGCTCCCGCCCTGCCTGCCTGGCCCCACCGAGGCCTGAGCAC GTTCCCGGGTGCCGAGCACTGCCTCCGGGCCTCCCCTGAGACCACACTCAGCAGTGGCTTCTTCGTTGCT GTAATTGAACGGGTCGAGGTGCCAAGCTCAGCCTCACAGGCCAAAGCATCAGCACCAGAACGCACACCCA GCCCAGCCCCAAAGAGAAAGAAGAGACAGCAAAGAGCCGCAGCCGGTGCTTGCACACCGCCTTGCACA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC200144 protein sequence
Red=Cloning site Green=Tags(s) MGLYAAAAGVLAGVESRQGSIKGLVYSSNFQNVKQLYALVCETQRYSAVLDAVIASAGLLRAEKKLRPHL AKVLVYELLLGKGFRGGGGRWKALLGRHQARLKAELARLKVHRGVSRNEDLLEVGSRPGPASQLPRFVRV NTLKTCSDDVVDYFKRQGFSYQGRASSLDDLRALKGKHFLLDPLMPELLVFPAQTDLHEHPLYRAGHLIL QDRASCLPAMLLDPPPGSHVIDACAAPGNKTSHLAALLKNQGKIFAFDLDAKRLASMATLLARAGVSCCE LAEEDFLAVSPSDPRYHEVHYILLDPSCSGSGMPSRQLEEPGAGTPSPVRLHALAGFQQRALCHALTFPS LQRLVYSTCSLCQEENEDVVRDALQQNPGAFRLAPALPAWPHRGLSTFPGAEHCLRASPETTLSSGFFVA VIERVEVPSSASQAKASAPERTPSPAPKRKKRQQRAAAGACTPPCT TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_148956 |
| ORF Size | 1398 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_148956.4 |
| RefSeq Size | 2377 bp |
| RefSeq ORF | 1401 bp |
| Locus ID | 55695 |
| UniProt ID | Q96P11 |
| Domains | Nol1_Nop2_Sun |
| MW | 50.4 kDa |
| Gene Summary | This gene encodes a member of an evolutionarily conserved family of proteins that may function as methyltransferases. This gene is located in a larger region of chromosome 7 that is deleted in Williams-Beuren syndrome, a multisystem developmental disorder. There are two pseudogenes for this gene located in the same region of chromosome 7. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2013] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC200144L1 | Lenti ORF clone of Human NOP2/Sun domain family, member 5 (NSUN5), transcript variant 1, Myc-DDK-tagged |
CNY 6,056.00 |
|
| RC200144L2 | Lenti ORF clone of Human NOP2/Sun domain family, member 5 (NSUN5), transcript variant 1, mGFP tagged |
CNY 5,890.00 |
|
| RC200144L3 | Lenti ORF clone of Human NOP2/Sun domain family, member 5 (NSUN5), transcript variant 1, Myc-DDK-tagged |
CNY 6,056.00 |
|
| RC200144L4 | Lenti ORF clone of Human NOP2/Sun domain family, member 5 (NSUN5), transcript variant 1, mGFP tagged |
CNY 5,890.00 |
|
| RG200144 | NSUN5 (tGFP-tagged) - Human NOP2/Sun domain family, member 5 (NSUN5), transcript variant 1 |
CNY 5,256.00 |
|
| SC319490 | NSUN5 (untagged)-Human NOP2/Sun domain family, member 5 (NSUN5), transcript variant 1 |
CNY 3,656.00 |
