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FBXL12 (NM_017703) Human Tagged ORF Clone
CAT＃: RC200178
FBXL12 (Myc-DDK-tagged)-Human F-box and leucine-rich repeat protein 12 (FBXL12)
ORF Plasmid: tGFP
"NM_017703" in other vectors (6)
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CNY 2,400.00
CNY 4,465.00
Product images
CNY 300.00
CNY 6,281.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | Fbl12 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC200178 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGACTTTGGTCGAACTGCCGGACTCGGTCCTGCTCGAGATCTTCTCTTACCTCCCGGTACGGGACC GGATCCGCATCTCCAGGGTCTGTCACCGCTGGAAGAGGCTGGTGGACGACCGGTGGCTGTGGCGACATGT CGACCTGACGCTCTACACGATGCGACCTAAAGTCATGTGGCACCTCCTTCGAAGGTACATGGCATCCCGG CTCCATTCCCTGCGGATGGGTGGCTACCTGTTCTCTGGCTCCCAGGCCCCCCAGTTGTCCCCTGCTCTGT TGAGAGCCCTGGGCCAGAAGTGCCCCAACCTGAAGCGCCTCTGCCTGCATGTGGCCGACCTGAGCATGGT GCCCATCACCAGCCTGCCCAGCACCTTGAGGACCCTGGAGCTGCACAGCTGCGAGATCTCCATGGCCTGG CTCCACAAGCAGCAGGACCCCACCGTGCTGCCCCTGCTTGAATGCATCGTGCTGGACCGCGTCCCCGCCT TCCGTGACGAGCACCTGCAGGGCCTGACGCGCTTCCGGGCCTTGCGCTCGCTGGTGCTGGGTGGTACCTA CCGTGTGACCGAGACAGGGCTGGATGCTGGCCTGCAGGAGCTCAGCTATCTGCAGAGGCTTGAGGTGCTG GGCTGCACCCTGTCTGCCGACAGCACCCTGCTGGCCATCAGCCGCCACCTCCGAGATGTGCGCAAGATCC GGCTGACCGTGAGGGGCCTCTCTGCCCCTGGCCTGGCTGTGCTGGAGGGAATGCCGGCCCTGGAGAGTCT GTGCCTGCAGGGTCCCCTCGTCACCCCAGAAATGCCCTCCCCCACTGAAATCCTCTCCTCCTGCCTCACT ATGCCCAAGCTCAGAGTCCTTGAGCTGCAGGGGCTGGGGTGGGAGGGTCAGGAGGCGGAGAAGATCCTGT GTAAGGGGCTGCCCCACTGTATGGTCATCGTCAGGGCTTGCCCCAAAGAGTCTATGGACTGGTGGATG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC200178 protein sequence
Red=Cloning site Green=Tags(s) MATLVELPDSVLLEIFSYLPVRDRIRISRVCHRWKRLVDDRWLWRHVDLTLYTMRPKVMWHLLRRYMASR LHSLRMGGYLFSGSQAPQLSPALLRALGQKCPNLKRLCLHVADLSMVPITSLPSTLRTLELHSCEISMAW LHKQQDPTVLPLLECIVLDRVPAFRDEHLQGLTRFRALRSLVLGGTYRVTETGLDAGLQELSYLQRLEVL GCTLSADSTLLAISRHLRDVRKIRLTVRGLSAPGLAVLEGMPALESLCLQGPLVTPEMPSPTEILSSCLT MPKLRVLELQGLGWEGQEAEKILCKGLPHCMVIVRACPKESMDWWM TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_017703 |
| ORF Size | 978 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_017703.3 |
| RefSeq Size | 1876 bp |
| RefSeq ORF | 981 bp |
| Locus ID | 54850 |
| UniProt ID | Q9NXK8 |
| Domains | F-box |
| Protein Families | Druggable Genome |
| MW | 37 kDa |
| Gene Summary | Members of the F-box protein family, such as FBXL12, are characterized by an approximately 40-amino acid F-box motif. SCF complexes, formed by SKP1 (MIM 601434), cullin (see CUL1; MIM 603134), and F-box proteins, act as protein-ubiquitin ligases. F-box proteins interact with SKP1 through the F box, and they interact with ubiquitination targets through other protein interaction domains (Jin et al., 2004 [PubMed 15520277]).[supplied by OMIM, Mar 2008] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC200178L1 | Lenti ORF clone of Human F-box and leucine-rich repeat protein 12 (FBXL12), Myc-DDK-tagged |
CNY 4,800.00 |
|
| RC200178L2 | Lenti ORF clone of Human F-box and leucine-rich repeat protein 12 (FBXL12), mGFP tagged |
CNY 5,890.00 |
|
| RC200178L3 | Lenti ORF clone of Human F-box and leucine-rich repeat protein 12 (FBXL12), Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC200178L4 | Lenti ORF clone of Human F-box and leucine-rich repeat protein 12 (FBXL12), mGFP tagged |
CNY 5,890.00 |
|
| RG200178 | FBXL12 (tGFP-tagged) - Human F-box and leucine-rich repeat protein 12 (FBXL12) |
CNY 4,000.00 |
|
| SC113988 | FBXL12 (untagged)-Human F-box and leucine-rich repeat protein 12 (FBXL12) |
CNY 2,400.00 |
