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PDZK1 (NM_002614) Human Tagged ORF Clone
CAT＃: RC200444
PDZK1 (Myc-DDK-tagged)-Human PDZ domain containing 1 (PDZK1), transcript variant 1
ORF Plasmid: tGFP
"NM_002614" in other vectors (6)
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CNY 3,864.00
CNY 5,320.00
Product images
CNY 300.00
CNY 1,999.00
CNY 2,700.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | CAP70; CLAMP; NHERF-3; NHERF3; PDZD1 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC200444 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGACCTCCACCTTCAACCCCCGAGAATGTAAACTGTCCAAGCAAGAAGGGCAAAACTATGGCTTCTTCC TGCGAATTGAGAAGGACACCGAGGGCCACCTGGTCCGGGTGGTTGAGAAGTGTAGCCCAGCAGAGAAGGC TGGCCTTCAAGATGGAGACAGAGTTCTTAGGATCAATGGTGTCTTTGTGGACAAAGAAGAACATATGCAG GTTGTGGATCTGGTCAGAAAGAGTGGGAATTCAGTGACTTTACTAGTTCTGGATGGGGATTCCTATGAGA AAGCAGTGAAAACACGGGTGGACTTGAAAGAGTTGGGTCAAAGTCAGAAGGAGCAAGGTTTGAGTGATAA TATACTTTCCCCTGTGATGAATGGAGGTGTGCAAACTTGGACCCAGCCCCGGCTCTGCTATCTCGTGAAG GAAGGAGGCAGCTATGGCTTCTCTCTGAAAACTGTCCAAGGTAAAAAGGGGGTGTACATGACTGATATTA CACCTCAAGGTGTGGCTATGAGAGCTGGAGTTCTGGCTGATGATCACTTGATTGAAGTGAATGGAGAGAA TGTAGAGGATGCCAGCCATGAGGAAGTGGTTGAAAAGGTGAAGAAGTCAGGAAGCCGTGTCATGTTCCTG CTGGTGGACAAAGAAACTGACAAGCGTCATGTTGAGCAGAAGATACAATTCAAAAGAGAAACAGCCAGTT TGAAACTGTTACCCCACCAGCCCCGAATTGTGGAGATGAAGAAAGGAAGCAATGGCTATGGTTTCTATCT GAGGGCAGGCTCAGAACAGAAAGGTCAAATCATCAAGGACATAGATTCTGGAAGTCCAGCAGAGGAGGCT GGCTTGAAGAACAATGATCTGGTAGTTGCTGTCAACGGCGAGTCTGTGGAAACCCTGGATCATGACAGTG TGGTAGAAATGATTAGAAAGGGTGGAGATCAGACTTCACTGTTGGTGGTAGACAAAGAGACGGACAACAT GTACAGACTGGCTCATTTTTCTCCATTTCTCTACTATCAAAGTCAAGAACTGCCCAATGGCTCTGTCAAG GAGGCTCCAGCTCCTACTCCCACTTCTCTGGAAGTCTCAAGTCCACCAGATACTACAGAGGAAGTAGATC ATAAGCCTAAACTCTGCAGGCTGGCTAAAGGTGAAAATGGCTATGGCTTTCACTTAAATGCGATTCGGGG TCTGCCAGGCTCATTCATCAAAGAGGTACAGAAGGGCGGTCCTGCTGACTTGGCTGGGCTAGAGGATGAG GATGTCATCATTGAAGTGAATGGGGTGAATGTGCTAGATGAACCCTATGAGAAGGTGGTGGATAGAATCC AGAGCAGTGGGAAGAATGTCACACTTCTAGTCTGTGGAAAGAAGGCCTATGATTATTTCCAAGCTAAGAA AATCCCTATTGTTTCCTCCCTGGCTGATCCACTTGACACCCCTCCAGATTCTAAAGAAGGAATAGTGGTG GAGTCAAACCATGACTCGCACATGGCAAAAGAACGGGCCCACAGTACAGCCTCACATTCTTCTTCCAATT CTGAAGATACAGAGATG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC200444 protein sequence
Red=Cloning site Green=Tags(s) MTSTFNPRECKLSKQEGQNYGFFLRIEKDTEGHLVRVVEKCSPAEKAGLQDGDRVLRINGVFVDKEEHMQ VVDLVRKSGNSVTLLVLDGDSYEKAVKTRVDLKELGQSQKEQGLSDNILSPVMNGGVQTWTQPRLCYLVK EGGSYGFSLKTVQGKKGVYMTDITPQGVAMRAGVLADDHLIEVNGENVEDASHEEVVEKVKKSGSRVMFL LVDKETDKRHVEQKIQFKRETASLKLLPHQPRIVEMKKGSNGYGFYLRAGSEQKGQIIKDIDSGSPAEEA GLKNNDLVVAVNGESVETLDHDSVVEMIRKGGDQTSLLVVDKETDNMYRLAHFSPFLYYQSQELPNGSVK EAPAPTPTSLEVSSPPDTTEEVDHKPKLCRLAKGENGYGFHLNAIRGLPGSFIKEVQKGGPADLAGLEDE DVIIEVNGVNVLDEPYEKVVDRIQSSGKNVTLLVCGKKAYDYFQAKKIPIVSSLADPLDTPPDSKEGIVV ESNHDSHMAKERAHSTASHSSSNSEDTEM TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_002614 |
| ORF Size | 1557 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_002614.4 |
| RefSeq Size | 2301 bp |
| RefSeq ORF | 1560 bp |
| Locus ID | 5174 |
| UniProt ID | Q5T2W1 |
| Domains | PDZ |
| MW | 57.1 kDa |
| Gene Summary | This gene encodes a PDZ domain-containing scaffolding protein. PDZ domain-containing molecules bind to and mediate the subcellular localization of target proteins. The encoded protein mediates the localization of cell surface proteins and plays a critical role in cholesterol metabolism by regulating the HDL receptor, scavenger receptor class B type 1. Single nucleotide polymorphisms in this gene may be associated with metabolic syndrome, and overexpression of this gene may play a role in drug resistance of multiple myeloma. Pseudogenes of this gene are located on the long arm of chromosome 1. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Jan 2011] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC200444L1 | Lenti ORF clone of Human PDZ domain containing 1 (PDZK1), transcript variant 1, Myc-DDK-tagged |
CNY 6,264.00 |
|
| RC200444L2 | Lenti ORF clone of Human PDZ domain containing 1 (PDZK1), transcript variant 1, mGFP tagged |
CNY 5,890.00 |
|
| RC200444L3 | Lenti ORF clone of Human PDZ domain containing 1 (PDZK1), transcript variant 1, Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC200444L4 | Lenti ORF clone of Human PDZ domain containing 1 (PDZK1), transcript variant 1, mGFP tagged |
CNY 5,890.00 |
|
| RG200444 | PDZK1 (tGFP-tagged) - Human PDZ domain containing 1 (PDZK1), transcript variant 1 |
CNY 5,464.00 |
|
| SC127846 | PDZK1 (untagged)-Human PDZ domain containing 1 (PDZK1), transcript variant 1 |
CNY 3,872.00 |
