CD82 (NM_002231) Human Tagged ORF Clone
CAT#: RC200457
CD82 (Myc-DDK-tagged)-Human CD82 molecule (CD82), transcript variant 1
ORF Plasmid: tGFP
"NM_002231" in other vectors (7)
Need custom modification / cloning service?
Get a free quote
CNY 2,400.00
CNY 4,465.00
Cited in 1 publication. |
CNY 300.00
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | 4F9; C33; GR15; IA4; KAI1; R2; SAR2; ST6; TSPAN27 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RC200457 representing NM_002231
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGCTCAGCCTGTATCAAAGTCACCAAATACTTTCTCTTCCTCTTCAACTTGATCTTCTTTATCCTGG GCGCAGTGATCCTGGGCTTCGGGGTGTGGATCCTGGCCGACAAGAGCAGTTTCATCTCTGTCCTGCAAAC CTCCTCCAGCTCGCTTAGGATGGGGGCCTATGTCTTCATCGGCGTGGGGGCAGTCACTATGCTCATGGGC TTCCTGGGCTGCATCGGCGCCGTCAACGAGGTCCGCTGCCTGCTGGGGCTGTACTTTGCTTTCCTGCTCC TGATCCTCATTGCCCAGGTGACGGCCGGGGCCCTCTTCTACTTCAACATGGGCAAGCTGAAGCAGGAGAT GGGCGGCATCGTGACTGAGCTCATTCGAGACTACAACAGCAGTCGCGAGGACAGCCTGCAGGATGCCTGG GACTACGTGCAGGCTCAGGTGAAGTGCTGCGGCTGGGTCAGCTTCTACAACTGGACAGACAACGCTGAGC TCATGAATCGCCCTGAGGTCACCTACCCCTGTTCCTGCGAAGTCAAGGGGGAAGAGGACAACAGCCTTTC TGTGAGGAAGGGCTTCTGCGAGGCCCCCGGCAACAGGACCCAGAGTGGCAACCACCCTGAGGACTGGCCT GTGTACCAGGAGGGCTGCATGGAGAAGGTGCAGGCGTGGCTGCAGGAGAACCTGGGCATCATCCTCGGCG TGGGCGTGGGTGTGGCCATCGTCGAGCTCCTGGGGATGGTCCTGTCCATCTGCTTGTGCCGGCACGTCCA TTCCGAAGACTACAGCAAGGTCCCCAAGTAC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC200457 representing NM_002231
Red=Cloning site Green=Tags(s) MGSACIKVTKYFLFLFNLIFFILGAVILGFGVWILADKSSFISVLQTSSSSLRMGAYVFIGVGAVTMLMG FLGCIGAVNEVRCLLGLYFAFLLLILIAQVTAGALFYFNMGKLKQEMGGIVTELIRDYNSSREDSLQDAW DYVQAQVKCCGWVSFYNWTDNAELMNRPEVTYPCSCEVKGEEDNSLSVRKGFCEAPGNRTQSGNHPEDWP VYQEGCMEKVQAWLQENLGIILGVGVGVAIVELLGMVLSICLCRHVHSEDYSKVPKY TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
![]() Sequencher program is needed, download here. |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
![]() |
ACCN | NM_002231 |
ORF Size | 801 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_002231.4 |
RefSeq Size | 1715 bp |
RefSeq ORF | 804 bp |
Locus ID | 3732 |
UniProt ID | P27701 |
Domains | transmembrane4 |
Protein Families | Druggable Genome, Transmembrane |
Protein Pathways | p53 signaling pathway |
MW | 29.4 kDa |
Gene Summary | This metastasis suppressor gene product is a membrane glycoprotein that is a member of the transmembrane 4 superfamily. Expression of this gene has been shown to be downregulated in tumor progression of human cancers and can be activated by p53 through a consensus binding sequence in the promoter. Its expression and that of p53 are strongly correlated, and the loss of expression of these two proteins is associated with poor survival for prostate cancer patients. Two alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, Jul 2008] |
Citations (1)
The use of this cDNA Clones has been cited in the following citations: |
---|
CD82 suppresses CD44 alternative splicing-dependent melanoma metastasis by mediating U2AF2 ubiquitination and degradation
,Zhang, P;Feng, S;Liu, G;Wang, H;Fu, A;Zhu, H;Ren, Q;Wang, B;Xu, X;Bai, H;Dong, C;,
Oncogene
,PubMed ID 27041584
[CD82]
|
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC200457L1 | Lenti ORF clone of Human CD82 molecule (CD82), transcript variant 1, Myc-DDK-tagged |
CNY 4,800.00 |
|
RC200457L2 | Lenti ORF clone of Human CD82 molecule (CD82), transcript variant 1, mGFP tagged |
CNY 5,890.00 |
|
RC200457L3 | Lenti ORF clone of Human CD82 molecule (CD82), transcript variant 1, Myc-DDK-tagged |
CNY 5,890.00 |
|
RC200457L4 | Lenti ORF clone of Human CD82 molecule (CD82), transcript variant 1, mGFP tagged |
CNY 4,800.00 |
|
RG200457 | CD82 (tGFP-tagged) - Human CD82 molecule (CD82), transcript variant 1 |
CNY 4,000.00 |
|
SC107926 | CD82 (untagged)-Human CD82 molecule (CD82), transcript variant 1 |
CNY 2,400.00 |
|
SC324395 | CD82 (untagged)-Human CD82 molecule (CD82), transcript variant 1 |
CNY 2,400.00 |