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HEXB (NM_000521) Human Tagged ORF Clone
CAT＃: RC200465
HEXB (Myc-DDK-tagged)-Human hexosaminidase B (beta polypeptide) (HEXB)
ORF Plasmid: tGFP
"NM_000521" in other vectors (6)
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CNY 6,208.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | ENC-1AS; HEL-248; HEL-S-111 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC200465 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGCTGTGCGGGCTGGGGCTGCCCCGGCCGCCCATGCTGCTGGCGCTGCTGTTGGCGACACTGCTGG CGGCGATGTTGGCGCTGCTGACTCAGGTGGCGCTGGTGGTGCAGGTGGCGGAGGCGGCTCGGGCCCCGAG CGTCTCGGCCAAGCCGGGGCCGGCGCTGTGGCCCCTGCCGCTCTCGGTGAAGATGACCCCGAACCTGCTG CATCTCGCCCCGGAGAACTTCTACATCAGCCACAGCCCCAATTCCACGGCGGGCCCCTCCTGCACCCTGC TGGAGGAAGCGTTTCGACGATATCATGGCTATATTTTTGGTTTCTACAAGTGGCATCATGAACCTGCTGA ATTCCAGGCTAAAACCCAGGTTCAGCAACTTCTTGTCTCAATCACCCTTCAGTCAGAGTGTGATGCTTTC CCCAACATATCTTCAGATGAGTCTTATACTTTACTTGTGAAAGAACCAGTGGCTGTCCTTAAGGCCAACA GAGTTTGGGGAGCATTACGAGGTTTAGAGACCTTTAGCCAGTTAGTTTATCAAGATTCTTATGGAACTTT CACCATCAATGAATCCACCATTATTGATTCTCCAAGGTTTTCTCACAGAGGAATTTTGATTGATACATCC AGACATTATCTGCCAGTTAAGATTATTCTTAAAACTCTGGATGCCATGGCTTTTAATAAGTTTAATGTTC TTCACTGGCACATAGTTGATGACCAGTCTTTCCCATATCAGAGCATCACTTTTCCTGAGTTAAGCAATAA AGGAAGCTATTCTTTGTCTCATGTTTATACACCAAATGATGTCCGTATGGTGATTGAATATGCCAGATTA CGAGGAATTCGAGTCCTGCCAGAATTTGATACCCCTGGGCATACACTATCTTGGGGAAAAGGTCAGAAAG ACCTCCTGACTCCATGTTACAGTAGACAAAACAAGTTGGACTCTTTTGGACCTATAAACCCTACTCTGAA TACAACATACAGCTTCCTTACTACATTTTTCAAAGAAATTAGTGAGGTGTTTCCAGATCAATTCATTCAT TTGGGAGGAGATGAAGTGGAATTTAAATGTTGGGAATCAAATCCAAAAATTCAAGATTTCATGAGGCAAA AAGGCTTTGGCACAGATTTTAAGAAACTAGAATCTTTCTACATTCAAAAGGTTTTGGATATTATTGCAAC CATAAACAAGGGATCCATTGTCTGGCAGGAGGTTTTTGATGATAAAGCAAAGCTTGCGCCGGGCACAATA GTTGAAGTATGGAAAGACAGCGCATATCCTGAGGAACTCAGTAGAGTCACAGCATCTGGCTTCCCTGTAA TCCTTTCTGCTCCTTGGTACTTAGATTTGATTAGCTATGGACAAGATTGGAGGAAATACTATAAAGTGGA ACCTCTTGATTTTGGCGGTACTCAGAAACAGAAACAACTTTTCATTGGTGGAGAAGCTTGTCTATGGGGA GAATATGTGGATGCAACTAACCTCACTCCAAGATTATGGCCTCGGGCAAGTGCTGTTGGTGAGAGACTCT GGAGTTCCAAAGATGTCAGAGATATGGATGACGCCTATGACAGACTGACAAGGCACCGCTGCAGGATGGT CGAACGTGGAATAGCTGCACAACCTCTTTATGCTGGATATTGTAACCATGAGAACATG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC200465 protein sequence
Red=Cloning site Green=Tags(s) MELCGLGLPRPPMLLALLLATLLAAMLALLTQVALVVQVAEAARAPSVSAKPGPALWPLPLSVKMTPNLL HLAPENFYISHSPNSTAGPSCTLLEEAFRRYHGYIFGFYKWHHEPAEFQAKTQVQQLLVSITLQSECDAF PNISSDESYTLLVKEPVAVLKANRVWGALRGLETFSQLVYQDSYGTFTINESTIIDSPRFSHRGILIDTS RHYLPVKIILKTLDAMAFNKFNVLHWHIVDDQSFPYQSITFPELSNKGSYSLSHVYTPNDVRMVIEYARL RGIRVLPEFDTPGHTLSWGKGQKDLLTPCYSRQNKLDSFGPINPTLNTTYSFLTTFFKEISEVFPDQFIH LGGDEVEFKCWESNPKIQDFMRQKGFGTDFKKLESFYIQKVLDIIATINKGSIVWQEVFDDKAKLAPGTI VEVWKDSAYPEELSRVTASGFPVILSAPWYLDLISYGQDWRKYYKVEPLDFGGTQKQKQLFIGGEACLWG EYVDATNLTPRLWPRASAVGERLWSSKDVRDMDDAYDRLTRHRCRMVERGIAAQPLYAGYCNHENM TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_000521 |
| ORF Size | 1668 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_000521.3 |
| RefSeq Size | 1919 bp |
| RefSeq ORF | 1671 bp |
| Locus ID | 3074 |
| UniProt ID | P07686 |
| Domains | Glyco_hydro_20 |
| Protein Families | Druggable Genome, Transmembrane |
| Protein Pathways | Amino sugar and nucleotide sugar metabolism, Glycosaminoglycan degradation, Glycosphingolipid biosynthesis - ganglio series, Glycosphingolipid biosynthesis - globo series, Lysosome, Metabolic pathways, Other glycan degradation |
| MW | 63.1 kDa |
| Gene Summary | Hexosaminidase B is the beta subunit of the lysosomal enzyme beta-hexosaminidase that, together with the cofactor GM2 activator protein, catalyzes the degradation of the ganglioside GM2, and other molecules containing terminal N-acetyl hexosamines. Beta-hexosaminidase is composed of two subunits, alpha and beta, which are encoded by separate genes. Both beta-hexosaminidase alpha and beta subunits are members of family 20 of glycosyl hydrolases. Mutations in the alpha or beta subunit genes lead to an accumulation of GM2 ganglioside in neurons and neurodegenerative disorders termed the GM2 gangliosidoses. Beta subunit gene mutations lead to Sandhoff disease (GM2-gangliosidosis type II). Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, May 2014] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC200465L1 | Lenti ORF clone of Human hexosaminidase B (beta polypeptide) (HEXB), Myc-DDK-tagged |
CNY 8,608.00 |
|
| RC200465L2 | Lenti ORF clone of Human hexosaminidase B (beta polypeptide) (HEXB), mGFP tagged |
CNY 5,890.00 |
|
| RC200465L3 | Lenti ORF clone of Human hexosaminidase B (beta polypeptide) (HEXB), Myc-DDK-tagged |
CNY 8,608.00 |
|
| RC200465L4 | Lenti ORF clone of Human hexosaminidase B (beta polypeptide) (HEXB), mGFP tagged |
CNY 8,608.00 |
|
| RG200465 | HEXB (tGFP-tagged) - Human hexosaminidase B (beta polypeptide) (HEXB) |
CNY 7,808.00 |
|
| SC321203 | HEXB (untagged)-Human hexosaminidase B (beta polypeptide) (HEXB) |
CNY 4,152.00 |
