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GMDS (NM_001500) Human Tagged ORF Clone
CAT＃: RC200471
GMDS (Myc-DDK-tagged)-Human GDP-mannose 4,6-dehydratase (GMDS)
ORF Plasmid: tGFP
"NM_001500" in other vectors (5)
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CNY 3,656.00
CNY 5,320.00
Product images
CNY 300.00
CNY 1,999.00
CNY 2,700.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | GMD; SDR3E1 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC200471 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCACACGCACCGGCACGCTGCCCCAGCGCCCGGGGCTCCGGGGACGGCGAGATGGGCAAGCCCAGGA ACGTGGCGCTCATCACCGGTATCACAGGCCAGGATGGTTCCTACCTGGCTGAGTTCCTGCTGGAGAAAGG CTATGAGGTCCATGGAATTGTACGGCGGTCCAGTTCATTTAATACGGGTCGAATTGAGCATCTGTATAAG AATCCCCAGGCTCACATTGAAGGAAACATGAAGTTGCACTATGGCGATCTCACTGACAGTACCTGCCTTG TGAAGATCATTAATGAAGTAAAGCCCACAGAGATCTACAACCTTGGAGCCCAGAGCCACGTCAAAATTTC CTTTGACCTCGCTGAGTACACTGCGGACGTTGACGGAGTTGGCACTCTACGACTTCTAGATGCAGTTAAG ACTTGTGGCCTTATCAACTCTGTGAAGTTCTACCAAGCCTCAACAAGTGAACTTTATGGGAAAGTGCAGG AAATACCCCAGAAGGAGACCACCCCTTTCTATCCCCGGTCACCCTATGGGGCAGCAAAACTCTATGCCTA TTGGATTGTGGTGAACTTCCGTGAGGCGTATAATCTCTTTGCAGTGAACGGCATTCTCTTCAATCATGAG AGTCCCAGAAGAGGAGCTAATTTCGTTACTCGAAAAATTAGCCGGTCAGTAGCTAAGATTTACCTTGGAC AACTGGAATGTTTCAGTTTGGGAAATCTGGATGCCAAACGAGATTGGGGCCATGCCAAGGACTATGTGGA GGCTATGTGGTTGATGTTGCAGAATGACGAGCCGGAGGACTTCGTTATAGCTACTGGGGAGGTCCATAGT GTCCGGGAATTTGTCGAGAAATCATTCTTGCACATTGGAAAAACCATTGTGTGGGAAGGAAAGAATGAAA ATGAAGTGGGCAGATGTAAAGAGACCGGCAAAGTTCACGTGACTGTGGATCTCAAGTACTACCGGCCAAC TGAAGTGGACTTTCTGCAGGGCGACTGCACCAAAGCGAAACAGAAGCTGAACTGGAAGCCCCGGGTCGCT TTCGATGAGCTGGTGAGGGAGATGGTGCACGCCGACGTGGAGCTCATGAGGACAAACCCCAATGCC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC200471 protein sequence
Red=Cloning site Green=Tags(s) MAHAPARCPSARGSGDGEMGKPRNVALITGITGQDGSYLAEFLLEKGYEVHGIVRRSSSFNTGRIEHLYK NPQAHIEGNMKLHYGDLTDSTCLVKIINEVKPTEIYNLGAQSHVKISFDLAEYTADVDGVGTLRLLDAVK TCGLINSVKFYQASTSELYGKVQEIPQKETTPFYPRSPYGAAKLYAYWIVVNFREAYNLFAVNGILFNHE SPRRGANFVTRKISRSVAKIYLGQLECFSLGNLDAKRDWGHAKDYVEAMWLMLQNDEPEDFVIATGEVHS VREFVEKSFLHIGKTIVWEGKNENEVGRCKETGKVHVTVDLKYYRPTEVDFLQGDCTKAKQKLNWKPRVA FDELVREMVHADVELMRTNPNA TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001500 |
| ORF Size | 1116 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_001500.4 |
| RefSeq Size | 1700 bp |
| RefSeq ORF | 1119 bp |
| Locus ID | 2762 |
| UniProt ID | O60547 |
| Protein Families | Druggable Genome |
| Protein Pathways | Amino sugar and nucleotide sugar metabolism, Fructose and mannose metabolism, Metabolic pathways |
| MW | 41.9 kDa |
| Gene Summary | GDP-mannose 4,6-dehydratase (GMD; EC 4.2.1.47) catalyzes the conversion of GDP-mannose to GDP-4-keto-6-deoxymannose, the first step in the synthesis of GDP-fucose from GDP-mannose, using NADP+ as a cofactor. The second and third steps of the pathway are catalyzed by a single enzyme, GDP-keto-6-deoxymannose 3,5-epimerase, 4-reductase, designated FX in humans (MIM 137020).[supplied by OMIM, Aug 2009] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC200471L3 | Lenti ORF clone of Human GDP-mannose 4,6-dehydratase (GMDS), Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC200471L4 | Lenti ORF clone of Human GDP-mannose 4,6-dehydratase (GMDS), mGFP tagged |
CNY 5,890.00 |
|
| RG200471 | GMDS (tGFP-tagged) - Human GDP-mannose 4,6-dehydratase (GMDS) |
CNY 5,256.00 |
|
| SC119207 | GMDS (untagged)-Human GDP-mannose 4,6-dehydratase (GMDS) |
CNY 3,656.00 |
|
| SC324372 | GMDS (untagged)-Human GDP-mannose 4,6-dehydratase (GMDS) |
CNY 3,656.00 |
