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GATM (NM_001482) Human Tagged ORF Clone
CAT＃: RC200474
GATM (Myc-DDK-tagged)-Human glycine amidinotransferase (L-arginine:glycine amidinotransferase) (GATM), nuclear gene encoding mitochondrial protein
ORF Plasmid: tGFP
"NM_001482" in other vectors (6)
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CNY 3,656.00
CNY 5,320.00
Product images
CNY 300.00
CNY 1,999.00
CNY 2,700.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | AGAT; AT; CCDS3; FRTS1 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC200474 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCTGCGGGTGCGGTGTCTGCGCGGCGGGAGCCGCGGCGCCGAGGCGGTGCACTACATCGGATCTCGGC TTGGACGAACCTTGACAGGATGGGTGCAGCGAACTTTCCAGAGCACCCAGGCAGCTACGGCTTCCTCCCG GAACTCCTGTGCAGCTGACGACAAAGCCACTGAGCCTCTGCCCAAGGACTGCCCTGTCTCTTCTTACAAC GAATGGGACCCCTTAGAGGAAGTGATAGTGGGCAGAGCAGAAAACGCCTGTGTTCCACCGTTCACCATCG AGGTGAAGGCCAACACATATGAAAAGTACTGGCCATTTTACCAGAAGCAAGGAGGGCATTATTTTCCCAA AGATCATTTGAAAAAGGCTGTTGCTGAAATTGAAGAAATGTGCAATATTTTAAAAACGGAAGGAGTGACA GTAAGGAGGCCTGACCCCATTGACTGGTCATTGAAGTATAAAACTCCTGATTTTGAGTCTACGGGTTTAT ACAGTGCAATGCCTCGAGACATCCTGATAGTTGTGGGCAATGAGATTATCGAGGCTCCCATGGCATGGCG TTCACGCTTCTTTGAGTACCGAGCGTACAGGTCAATTATCAAAGACTACTTCCACCGTGGCGCCAAGTGG ACAACAGCTCCTAAGCCCACAATGGCTGATGAGCTTTATAACCAGGATTATCCCATCCACTCTGTAGAAG ACAGACACAAATTGGCTGCTCAGGGAAAATTTGTGACAACTGAGTTTGAGCCATGCTTTGATGCTGCTGA CTTCATTCGAGCTGGAAGAGATATTTTTGCACAGAGAAGCCAGGTTACAAACTACCTAGGCATTGAATGG ATGCGTAGGCATCTTGCTCCAGACTACAGAGTGCATATCATCTCCTTTAAAGATCCCAATCCCATGCATA TTGATGCTACCTTCAACATCATTGGACCTGGTATTGTGCTTTCCAACCCTGACCGACCATGTCACCAGAT TGATCTTTTCAAGAAAGCAGGATGGACTATCATTACTCCTCCAACACCAATCATCCCAGACGATCATCCA CTCTGGATGTCATCCAAATGGCTTTCCATGAATGTCTTAATGCTAGATGAAAAACGTGTTATGGTGGATG CCAATGAAGTTCCAATTCAAAAGATGTTTGAAAAGCTGGGTATCACTACCATTAAAGTTAACATTCGTAA TGCCAATTCCCTGGGAGGAGGCTTCCATTGCTGGACCTGCGATGTCCGGCGCCGAGGCACCTTACAGTCC TACTTGGAC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC200474 protein sequence
Red=Cloning site Green=Tags(s) MLRVRCLRGGSRGAEAVHYIGSRLGRTLTGWVQRTFQSTQAATASSRNSCAADDKATEPLPKDCPVSSYN EWDPLEEVIVGRAENACVPPFTIEVKANTYEKYWPFYQKQGGHYFPKDHLKKAVAEIEEMCNILKTEGVT VRRPDPIDWSLKYKTPDFESTGLYSAMPRDILIVVGNEIIEAPMAWRSRFFEYRAYRSIIKDYFHRGAKW TTAPKPTMADELYNQDYPIHSVEDRHKLAAQGKFVTTEFEPCFDAADFIRAGRDIFAQRSQVTNYLGIEW MRRHLAPDYRVHIISFKDPNPMHIDATFNIIGPGIVLSNPDRPCHQIDLFKKAGWTIITPPTPIIPDDHP LWMSSKWLSMNVLMLDEKRVMVDANEVPIQKMFEKLGITTIKVNIRNANSLGGGFHCWTCDVRRRGTLQS YLD TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001482 |
| ORF Size | 1269 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_001482.3 |
| RefSeq Size | 2602 bp |
| RefSeq ORF | 1272 bp |
| Locus ID | 2628 |
| UniProt ID | P50440 |
| Domains | Amidinotransf |
| Protein Families | Druggable Genome |
| Protein Pathways | Arginine and proline metabolism, Glycine, serine and threonine metabolism, Metabolic pathways |
| MW | 48.5 kDa |
| Gene Summary | This gene encodes a mitochondrial enzyme that belongs to the amidinotransferase family. This enzyme is involved in creatine biosynthesis, whereby it catalyzes the transfer of a guanido group from L-arginine to glycine, resulting in guanidinoacetic acid, the immediate precursor of creatine. Mutations in this gene cause arginine:glycine amidinotransferase deficiency, an inborn error of creatine synthesis characterized by cognitive disability, language impairment, and behavioral disorders. [provided by RefSeq, Jul 2008] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC200474L1 | Lenti ORF clone of Human glycine amidinotransferase (L-arginine:glycine amidinotransferase) (GATM), nuclear gene encoding mitochondrial protein, Myc-DDK-tagged |
CNY 6,056.00 |
|
| RC200474L2 | Lenti ORF clone of Human glycine amidinotransferase (L-arginine:glycine amidinotransferase) (GATM), nuclear gene encoding mitochondrial protein, mGFP tagged |
CNY 5,890.00 |
|
| RC200474L3 | Lenti ORF clone of Human glycine amidinotransferase (L-arginine:glycine amidinotransferase) (GATM), nuclear gene encoding mitochondrial protein, Myc-DDK-tagged |
CNY 6,056.00 |
|
| RC200474L4 | Lenti ORF clone of Human glycine amidinotransferase (L-arginine:glycine amidinotransferase) (GATM), nuclear gene encoding mitochondrial protein, mGFP tagged |
CNY 6,056.00 |
|
| RG200474 | GATM (tGFP-tagged) - Human glycine amidinotransferase (L-arginine:glycine amidinotransferase) (GATM), nuclear gene encoding mitochondrial protein |
CNY 5,256.00 |
|
| SC321307 | GATM (untagged)-Human glycine amidinotransferase (L-arginine:glycine amidinotransferase) (GATM), nuclear gene encoding mitochondrial protein |
CNY 3,656.00 |
