GALK2 (NM_002044) Human Tagged ORF Clone

CAT#: RC200475

GALK2 (Myc-DDK-tagged)-Human galactokinase 2 (GALK2), transcript variant 1

ORF Plasmid: DDK tGFP



  "NM_002044" in other vectors (6)

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CNY 3,656.00

CNY 5,320.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (5)
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

CNY 5,420.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00


DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 30 ul

CNY 300.00


GALK2 rabbit polyclonal antibody
    • 25 ul

CNY 800.00
CNY 1,280.00

Specifications

Product Data
Type Human Tagged ORF Clone
Tag Myc-DDK
Synonyms GK2
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RC200475 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCTACAGAGAGCCCTGCTACGCGTCGGGTCCAGGTGGCAGAACATCCTAGGTTACTGAAGCTAAAGG
AGATGTTTAACTCCAAGTTTGGATCTATTCCCAAGTTTTATGTTCGAGCACCAGGAAGAGTCAACATAAT
AGGAGAGCATATAGATTATTGTGGATATTCTGTTCTTCCTATGGCTGTAGAACAAGATGTGCTAATAGCT
GTAGAACCTGTGAAAACGTACGCTCTCCAACTGGCCAATACAAATCCCTTGTATCCGGACTTCAGTACTA
GTGCTAATAACATCCAGATTGATAAAACCAAGCCTTTGTGGCACAACTATTTCTTATGTGGACTTAAAGG
AATTCAGGAACACTTTGGTCTTAGTAACCTGACTGGAATGAACTGCCTGGTAGATGGAAATATCCCACCA
AGTTCTGGCCTCTCCAGCTCCAGTGCTTTGGTCTGTTGTGCTGGCTTGGTGACGCTCACAGTGCTGGGAA
GGAATCTATCCAAGGTGGAACTTGCAGAAATCTGTGCCAAGAGTGAGCGTTACATTGGCACTGAAGGAGG
AGGCATGGACCAGTCTATATCATTTCTTGCAGAAGAAGGAACTGCCAAGTTGATAGAATTTAGTCCTCTG
AGGGCAACCGATGTAAAACTCCCAAGTGGAGCAGTGTTTGTGATTGCCAACAGTTGTGTGGAGATGAATA
AGGCAGCAACTTCCCATTTCAATATCAGGGTGATGGAGTGTCGGCTGGCTGCGAAGCTCCTGGCTAAATA
CAAAAGCTTGCAATGGGACAAAGTACTGAGGCTGGAGGAGGTGCAGGCTAAACTAGGGATTAGTCTAGAA
GAAATGCTGTTGGTCACAGAAGATGCCCTTCATCCTGAACCCTATAACCCTGAGGAGATCTGCAGGTGTC
TGGGAATTAGCCTGGAGGAACTCCGAACCCAAATCCTGAGTCCAAACACTCAAGATGTGCTCATCTTCAA
ACTCTATCAGCGGGCAAAGCATGTGTACAGCGAGGCTGCGCGAGTGCTCCAGTTTAAGAAGATATGTGAA
GAAGCACCTGAAAACATGGTCCAGCTGCTGGGAGAGTTGATGAACCAGAGCCACATGAGCTGCCGGGACA
TGTATGAGTGCAGCTGCCCCGAGCTGGATCAGCTGGTGGACATCTGTCGGAAGTTTGGGGCTCAAGGGTC
ACGACTTACTGGAGCAGGATGGGGAGGCTGTACAGTATCAATGGTACCTGCGGACAAGCTGCCCAGCTTT
CTAGCAAATGTGCACAAAGCTTATTACCAGAGGAGTGATGGAAGCTTAGCACCGGAGAAGCAAAGTTTGT
TTGCTACCAAACCTGGAGGTGGGGCTTTGGTTTTGCTTGAGGCC


AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC
TGGATTACAAGGATGACGACGATAAG
GTTTAA
>RC200475 protein sequence
Red=Cloning site Green=Tags(s)

MATESPATRRVQVAEHPRLLKLKEMFNSKFGSIPKFYVRAPGRVNIIGEHIDYCGYSVLPMAVEQDVLIA
VEPVKTYALQLANTNPLYPDFSTSANNIQIDKTKPLWHNYFLCGLKGIQEHFGLSNLTGMNCLVDGNIPP
SSGLSSSSALVCCAGLVTLTVLGRNLSKVELAEICAKSERYIGTEGGGMDQSISFLAEEGTAKLIEFSPL
RATDVKLPSGAVFVIANSCVEMNKAATSHFNIRVMECRLAAKLLAKYKSLQWDKVLRLEEVQAKLGISLE
EMLLVTEDALHPEPYNPEEICRCLGISLEELRTQILSPNTQDVLIFKLYQRAKHVYSEAARVLQFKKICE
EAPENMVQLLGELMNQSHMSCRDMYECSCPELDQLVDICRKFGAQGSRLTGAGWGGCTVSMVPADKLPSF
LANVHKAYYQRSDGSLAPEKQSLFATKPGGGALVLLEA

SGPTRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-RsrII      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_002044
ORF Size 1374 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_002044.1
RefSeq Size 3330 bp
RefSeq ORF 1377 bp
Locus ID 2585
UniProt ID Q01415
Domains GHMP_kinases
Protein Families Druggable Genome
Protein Pathways Amino sugar and nucleotide sugar metabolism, Galactose metabolism, Metabolic pathways
MW 50.4 kDa
Gene Summary This gene encodes a highly efficient N-acetylgalactosamine (GalNAc) kinase, which has galactokinase activity when galactose is present at high concentrations. The encoded protein is a member of the GHMP kinase family. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jun 2017]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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