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FOXO1 (NM_002015) Human Tagged ORF Clone
CAT＃: RC200477
FOXO1 (Myc-DDK-tagged)-Human forkhead box O1 (FOXO1)
ORF Plasmid: tGFP
"NM_002015" in other vectors (6)
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CNY 7,312.00
| Cited in 7 publications. |
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | FKH1; FKHR; FOXO1A |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC200477 representing NM_002015
Red=Cloning site Blue=ORF Green=Tags(s) CTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCCGAGGCGCCTCAGGTGGTGGAGATCGACCCGGACTTCGAGCCGCTGCCCCGGCCGCGCTCGTGCA CCTGGCCGCTGCCCAGGCCGGAGTTTAGCCAGTCCAACTCGGCCACCTCCAGCCCGGCGCCGTCGGGCAG CGCGGCTGCCAACCCCGACGCCGCGGCGGGCCTGCCCTCGGCCTCGGCTGCCGCTGTCAGCGCCGACTTC ATGAGCAACCTGAGCTTGCTGGAGGAGAGCGAGGACTTCCCGCAGGCGCCCGGCTCCGTGGCGGCGGCGG TGGCGGCGGCGGCCGCCGCGGCCGCCACCGGGGGGCTGTGCGGGGACTTCCAGGGCCCGGAGGCGGGCTG CCTGCACCCAGCGCCACCGCAGCCCCCGCCGCCCGGGCCGCTGTCGCAGCACCCGCCGGTGCCCCCCGCC GCCGCTGGGCCGCTCGCGGGGCAGCCGCGCAAGAGCAGCTCGTCCCGCCGCAACGCGTGGGGCAACCTGT CCTACGCCGACCTCATCACCAAGGCCATCGAGAGCTCGGCGGAGAAGCGGCTCACGCTGTCGCAGATCTA CGAGTGGATGGTCAAGAGCGTGCCCTACTTCAAGGATAAGGGTGACAGCAACAGCTCGGCGGGCTGGAAG AATTCAATTCGTCATAATCTGTCCCTACACAGCAAGTTCATTCGTGTGCAGAATGAAGGAACTGGAAAAA GTTCTTGGTGGATGCTCAATCCAGAGGGTGGCAAGAGCGGGAAATCTCCTAGGAGAAGAGCTGCATCCAT GGACAACAACAGTAAATTTGCTAAGAGCCGAAGCCGAGCTGCCAAGAAGAAAGCATCTCTCCAGTCTGGC CAGGAGGGTGCTGGGGACAGCCCTGGATCACAGTTTTCCAAATGGCCTGCAAGCCCTGGCTCTCACAGCA ATGATGACTTTGATAACTGGAGTACATTTCGCCCTCGAACTAGCTCAAATGCTAGTACTATTAGTGGGAG ACTCTCACCCATTATGACCGAACAGGATGATCTTGGAGAAGGGGATGTGCATTCTATGGTGTACCCGCCA TCTGCCGCAAAGATGGCCTCTACTTTACCCAGTCTGTCTGAGATAAGCAATCCCGAAAACATGGAAAATC TTTTGGATAATCTCAACCTTCTCTCATCACCAACATCATTAACTGTTTCGACCCAGTCCTCACCTGGCAC CATGATGCAGCAGACGCCGTGCTACTCGTTTGCGCCACCAAACACCAGTTTGAATTCACCCAGCCCAAAC TACCAAAAATATACATATGGCCAATCCAGCATGAGCCCTTTGCCCCAGATGCCTATACAAACACTTCAGG ACAATAAGTCGAGTTATGGAGGTATGAGTCAGTATAACTGTGCGCCTGGACTCTTGAAGGAGTTGCTGAC TTCTGACTCTCCTCCCCATAATGACATTATGACACCAGTTGATCCTGGGGTAGCCCAGCCCAACAGCCGG GTTCTGGGCCAGAACGTCATGATGGGCCCTAATTCGGTCATGTCAACCTATGGCAGCCAGGTATCTCATA ACAAAATGATGAATCCCAGCTCCCATACCCACCCTGGACATGCTCAGCAGACATCTGCAGTTAACGGGCG TCCCCTGCCCCACACGGTAAGCACCATGCCCCACACCTCGGGTATGAACCGCCTGACCCAAGTGAAGACA CCTGTACAAGTGCCTCTGCCCCACCCCATGCAGATGAGTGCCCTGGGGGGCTACTCCTCCGTGAGCAGCT GCAATGGCTATGGCAGAATGGGCCTTCTCCACCAGGAGAAGCTCCCAAGTGACTTGGATGGCATGTTCAT TGAGCGCTTAGACTGTGACATGGAATCCATCATTCGGAATGACCTCATGGATGGAGATACATTGGATTTT AACTTTGACAATGTGTTGCCCAACCAAAGCTTCCCACACAGTGTCAAGACAACGACACATAGCTGGGTGT CAGGC CTCGAGCAGAAACTCATCTCTGAAGAGGATCTGGCAGCAAATGATATCCTGGATTACAAGGATGACGACG ATAAGGTTTAA >RC200477 representing NM_002015
Red=Cloning site Green=Tags(s) MAEAPQVVEIDPDFEPLPRPRSCTWPLPRPEFSQSNSATSSPAPSGSAAANPDAAAGLPSASAAAVSADF MSNLSLLEESEDFPQAPGSVAAAVAAAAAAAATGGLCGDFQGPEAGCLHPAPPQPPPPGPLSQHPPVPPA AAGPLAGQPRKSSSSRRNAWGNLSYADLITKAIESSAEKRLTLSQIYEWMVKSVPYFKDKGDSNSSAGWK NSIRHNLSLHSKFIRVQNEGTGKSSWWMLNPEGGKSGKSPRRRAASMDNNSKFAKSRSRAAKKKASLQSG QEGAGDSPGSQFSKWPASPGSHSNDDFDNWSTFRPRTSSNASTISGRLSPIMTEQDDLGEGDVHSMVYPP SAAKMASTLPSLSEISNPENMENLLDNLNLLSSPTSLTVSTQSSPGTMMQQTPCYSFAPPNTSLNSPSPN YQKYTYGQSSMSPLPQMPIQTLQDNKSSYGGMSQYNCAPGLLKELLTSDSPPHNDIMTPVDPGVAQPNSR VLGQNVMMGPNSVMSTYGSQVSHNKMMNPSSHTHPGHAQQTSAVNGRPLPHTVSTMPHTSGMNRLTQVKT PVQVPLPHPMQMSALGGYSSVSSCNGYGRMGLLHQEKLPSDLDGMFIERLDCDMESIIRNDLMDGDTLDF NFDNVLPNQSFPHSVKTTTHSWVSG LEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-XhoI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_002015 |
| ORF Size | 1965 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_002015.4 |
| RefSeq Size | 5723 bp |
| RefSeq ORF | 1968 bp |
| Locus ID | 2308 |
| UniProt ID | Q12778 |
| Domains | FH |
| Protein Families | Druggable Genome, Transcription Factors |
| Protein Pathways | Insulin signaling pathway, Pathways in cancer, Prostate cancer |
| MW | 69.5 kDa |
| Gene Summary | This gene belongs to the forkhead family of transcription factors which are characterized by a distinct forkhead domain. The specific function of this gene has not yet been determined; however, it may play a role in myogenic growth and differentiation. Translocation of this gene with PAX3 has been associated with alveolar rhabdomyosarcoma. [provided by RefSeq, Jul 2008] |
Citations (7)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
Uev1A promotes breast cancer cell survival and chemoresistance through the AKT-FOXO1-BIM pathway
,Wu, Z;Niu, T;Xiao, W;,
Cancer Cell Int.
,PubMed ID 31827405
[FOXO1]
|
|
The HDAC3-SMARCA4-miR-27a axis promotes expression of the PAX3:FOXO1 fusion oncogene in rhabdomyosarcoma
,Bharathy, N;Berlow, NE;Wang, E;Abraham, J;Settelmeyer, TP;Hooper, JE;Svalina, MN;Ishikawa, Y;Zientek, K;Bajwa, Z;Goros, MW;Hernandez, BS;Wolff, JE;Rudek, MA;Xu, L;Anders, NM;Pal, R;Harrold, AP;Davies, AM;Ashok, A;Bushby, D;Mancini, M;Noakes, C;Goodwin, NC;Ordentlich, P;Keck, J;Hawkins, DS;Rudzinski, ER;Chatterjee, B;Bächinger, HP;Barr, FG;Liddle, J;Garcia, BA;Mansoor, A;Perkins, TJ;Vakoc, CR;Michalek, JE;Keller, C;,
Sci Signal
,PubMed ID 30459282
[FOXO1]
|
|
Identification and Functional Analysis of a Novel G310D Variant in the Insulin-like Growth Factor 1 Receptor (IGF1R) Gene that Associates with Type 2 Diabetes in American Indians
,Muller, YL;Skelton, G;Piaggi, P;Chen, P;Nair, A;Kobes, S;Hsueh, WC;Knowler, WC;Hanson, RL;Baier, LJ;Bogardus, C;,
Diabetes Metab. Res. Rev.
,PubMed ID 29470850
[FOXO1]
|
|
Hyperactive FOXO1 results in lack of tip stalk identity and deficient microvascular regeneration during kidney injury
,Dang, LTH;Aburatani, T;Marsh, GA;Johnson, BG;Alimperti, S;Yoon, CJ;Huang, A;Szak, S;Nakagawa, N;Gomez, I;Ren, S;Read, SK;Sparages, C;Aplin, AC;Nicosia, RF;Chen, C;Ligresti, G;Duffield, JS;,
Biomaterials
,PubMed ID 28711779
[FOXO1]
|
|
Chronic Uridine Administration Induces Fatty Liver and Pre-Diabetic Conditions in Mice
,Urasaki, Y;Pizzorno, G;Le, TT;,
PLoS ONE
,PubMed ID 26789264
[FOXO1]
|
|
A functional interaction between Hippo-YAP signalling and FoxO1 mediates the oxidative stress response
,Shao, D;Zhai, P;Del Re, DP;Sciarretta, S;Yabuta, N;Nojima, H;Lim, DS;Pan, D;Sadoshima, J;,
Nat Commun, Feb 2014.
,PubMed ID 24525530
[FOXO1]
|
|
SIRT3 overexpression antagonizes high glucose accelerated cellular senescence in human diploid fibroblasts via the SIRT3-FOXO1 signaling pathway
,null,
Age
,PubMed ID 23494737
[FOXO1]
|
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC200477L1 | Lenti ORF clone of Human forkhead box O1 (FOXO1), Myc-DDK-tagged |
CNY 9,712.00 |
|
| RC200477L2 | Lenti ORF clone of Human forkhead box O1 (FOXO1), mGFP tagged |
CNY 9,712.00 |
|
| RC200477L3 | Lenti ORF clone of Human forkhead box O1 (FOXO1), Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC200477L4 | Lenti ORF clone of Human forkhead box O1 (FOXO1), mGFP tagged |
CNY 9,712.00 |
|
| RG200477 | FOXO1 (tGFP-tagged) - Human forkhead box O1 (FOXO1) |
CNY 8,912.00 |
|
| SC118890 | FOXO1 (untagged)-Human forkhead box O1 (FOXO1) |
CNY 7,328.00 |
