GFPT2 (NM_005110) Human Tagged ORF Clone
CAT#: RC200519
GFPT2 (Myc-DDK-tagged)-Human glutamine-fructose-6-phosphate transaminase 2 (GFPT2)
ORF Plasmid: tGFP
"NM_005110" in other vectors (6)
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CNY 5,000.00
CNY 5,320.00
CNY 300.00
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | GFAT; GFAT 2; GFAT2 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RC200519 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTGCGGAATCTTTGCCTACATGAACTACAGAGTCCCCCGGACGAGGAAGGAGATCTTCGAAACCCTCA TCAAGGGCCTGCAGCGGCTGGAGTACAGAGGCTACGACTCGGCAGGTGTGGCGATCGATGGGAATAATCA CGAAGTCAAAGAAAGACACATTCAGCTGGTCAAGAAAAGGGGGAAAGTCAAGGCTCTCGATGAAGAACTT TACAAACAAGACAGCATGGACTTAAAAGTGGAGTTTGAGACACACTTCGGCATTGCCCACACGCGCTGGG CCACCCACGGGGTCCCCAGTGCTGTCAACAGCCACCCTCAGCGCTCAGACAAAGGCAACGAATTTGTTGT CATCCACAATGGGATCATCACAAATTACAAAGATCTGAGGAAATTTCTGGAAAGCAAAGGCTACGAGTTT GAGTCAGAAACAGATACAGAGACCATCGCCAAGCTGATTAAATATGTGTTCGACAACAGAGAAACTGAGG ACATTACGTTTTCAACGTTGGTCGAGAGAGTCATTCAGCAGTTGGAAGGTGCATTCGCGCTGGTTTTCAA GAGTGTCCACTACCCAGGAGAAGCCGTTGCCACACGGAGAGGCAGCCCCCTGCTCATCGGAGTCCGGAGC AAATACAAGCTCTCCACAGAACAGATCCCTATCTTATACAGGACGTGCACTCTGGAGAATGTGAAGAATA TCTGTAAGACACGGATGAAGAGGCTGGACAGCTCCGCCTGCCTGCATGCTGTGGGCGACAAGGCCGTGGA ATTCTTCTTTGCTTCTGATGCAAGCGCTATCATAGAGCACACCAACCGGGTCATCTTCCTGGAGGACGAT GACATCGCCGCAGTGGCTGATGGGAAACTCTCCATTCACCGGGTCAAGCGCTCGGCCAGTGATGACCCAT CTCGAGCCATCCAGACCTTGCAGATGGAACTGCAGCAAATCATGAAAGGTAACTTCAGTGCGTTTATGCA GAAGGAGATCTTCGAACAGCCAGAATCAGTTTTCAATACTATGAGAGGTCGGGTGAATTTTGAAACCAAC ACAGTGCTCCTGGGTGGCTTGAAGGACCACTTGAAGGAGATTCGACGATGCCGACGGCTCATCGTGATTG GCTGTGGAACCAGCTACCACGCTGCCGTGGCTACGCGGCAAGTTTTGGAGGAACTGACTGAGCTTCCTGT GATGGTTGAACTTGCTAGTGATTTTCTGGACAGGAACACACCTGTGTTCAGGGATGACGTTTGCTTTTTC ATCAGCCAGTCAGGCGAGACCGCGGACACCCTCCTGGCGCTGCGCTACTGTAAGGACCGCGGCGCTCTCA CCGTGGGCGTCACCAACACCGTGGGCAGCTCCATCTCTCGCGAGACCGACTGCGGCGTCCACATCAACGC AGGGCCGGAGGTCGGCGTGGCCAGCACCAAGGCTTATACCAGTCAGTTCATCTCTCTGGTGATGTTTGGT TTGATGATGTCTGAAGACCGAATTTCACTACAAAACAGGAGGCAAGAGATCATCCGTGGCTTGAGATCTT TACCTGAGCTGATCAAGGAAGTGCTGTCTCTGGAGGAGAAGATCCACGACTTGGCCCTGGAGCTCTACAC GCAGAGATCGCTGCTGGTGATGGGGCGGGGCTACAACTATGCCACCTGCCTGGAAGGAGCCCTGAAAATT AAAGAGATAACCTACATGCACTCAGAAGGCATCCTGGCTGGGGAGCTGAAGCACGGGCCCCTGGCACTGA TTGACAAGCAGATGCCCGTCATCATGGTCATTATGAAGGATCCTTGCTTCGCCAAATGCCAGAACGCCCT GCAGCAAGTCACGGCCCGCCAGGGTCGCCCCATTATACTGTGCTCCAAGGACGATACTGAAAGTTCCAAG TTTGCGTATAAGACAATCGAGCTGCCCCACACTGTGGACTGCCTCCAGGGCATCCTGAGCGTGATTCCGC TGCAGCTGCTGTCCTTCCACCTGGCTGTTCTCCGAGGATATGACGTTGACTTCCCCAGAAATCTGGCCAA GTCTGTAACTGTGGAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC200519 protein sequence
Red=Cloning site Green=Tags(s) MCGIFAYMNYRVPRTRKEIFETLIKGLQRLEYRGYDSAGVAIDGNNHEVKERHIQLVKKRGKVKALDEEL YKQDSMDLKVEFETHFGIAHTRWATHGVPSAVNSHPQRSDKGNEFVVIHNGIITNYKDLRKFLESKGYEF ESETDTETIAKLIKYVFDNRETEDITFSTLVERVIQQLEGAFALVFKSVHYPGEAVATRRGSPLLIGVRS KYKLSTEQIPILYRTCTLENVKNICKTRMKRLDSSACLHAVGDKAVEFFFASDASAIIEHTNRVIFLEDD DIAAVADGKLSIHRVKRSASDDPSRAIQTLQMELQQIMKGNFSAFMQKEIFEQPESVFNTMRGRVNFETN TVLLGGLKDHLKEIRRCRRLIVIGCGTSYHAAVATRQVLEELTELPVMVELASDFLDRNTPVFRDDVCFF ISQSGETADTLLALRYCKDRGALTVGVTNTVGSSISRETDCGVHINAGPEVGVASTKAYTSQFISLVMFG LMMSEDRISLQNRRQEIIRGLRSLPELIKEVLSLEEKIHDLALELYTQRSLLVMGRGYNYATCLEGALKI KEITYMHSEGILAGELKHGPLALIDKQMPVIMVIMKDPCFAKCQNALQQVTARQGRPIILCSKDDTESSK FAYKTIELPHTVDCLQGILSVIPLQLLSFHLAVLRGYDVDFPRNLAKSVTVE TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
Sequencher program is needed, download here. |
Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
ACCN | NM_005110 |
ORF Size | 2046 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
Reference Data | |
RefSeq | NM_005110.4 |
RefSeq Size | 3011 bp |
RefSeq ORF | 2049 bp |
Locus ID | 9945 |
UniProt ID | O94808 |
Domains | GATase_2, SIS |
Protein Families | Protease |
Protein Pathways | Alanine, aspartate and glutamate metabolism, Amino sugar and nucleotide sugar metabolism, Metabolic pathways |
MW | 76.9 kDa |
Gene Summary | Controls the flux of glucose into the hexosamine pathway. Most likely involved in regulating the availability of precursors for N- and O-linked glycosylation of proteins.[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC200519L1 | Lenti ORF clone of Human glutamine-fructose-6-phosphate transaminase 2 (GFPT2), Myc-DDK-tagged |
CNY 7,400.00 |
|
RC200519L2 | Lenti ORF clone of Human glutamine-fructose-6-phosphate transaminase 2 (GFPT2), mGFP tagged |
CNY 7,400.00 |
|
RC200519L3 | Lenti ORF clone of Human glutamine-fructose-6-phosphate transaminase 2 (GFPT2), Myc-DDK-tagged |
CNY 7,700.00 |
|
RC200519L4 | Lenti ORF clone of Human glutamine-fructose-6-phosphate transaminase 2 (GFPT2), mGFP tagged |
CNY 7,700.00 |
|
RG200519 | GFPT2 (tGFP-tagged) - Human glutamine-fructose-6-phosphate transaminase 2 (GFPT2) |
CNY 6,600.00 |
|
SC321384 | GFPT2 (untagged)-Human glutamine-fructose-6-phosphate transaminase 2 (GFPT2) |
CNY 5,000.00 |