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ESD (NM_001984) Human Tagged ORF Clone
CAT＃: RC200533
ESD (Myc-DDK-tagged)-Human esterase D (ESD)
ORF Plasmid: tGFP
"NM_001984" in other vectors (7)
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CNY 2,400.00
CNY 4,465.00
| Cited in 1 publication. |
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | FGH |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC200533 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCATTGAAGCAGATTTCCAGCAACAAGTGCTTTGGGGGATTGCAGAAAGTTTTTGAACATGACAGTG TTGAACTAAACTGCAAAATGAAATTTGCTGTCTACTTACCACCAAAGGCAGAAACAGGAAAGTGCCCTGC ACTGTATTGGCTCTCAGGTTTAACTTGCACAGAGCAAAATTTTATATCAAAATCTGGTTATCATCAGTCT GCTTCAGAACATGGTCTTGTTGTCATTGCTCCAGATACCAGCCCTCGTGGCTGCAATATTAAAGGTGAAG ATGAGAGCTGGGACTTTGGCACTGGTGCTGGATTTTATGTTGATGCCACTGAAGATCCTTGGAAAACCAA CTACAGAATGTACTCTTATGTCACAGAGGAGCTTCCCCAACTCATAAATGCCAATTTTCCAGTGGATCCC CAAAGGATGTCTATTTTTGGCCACTCCATGGGAGGTCATGGAGCTCTGATCTGTGCTTTGAAAAATCCTG GAAAATACAAATCTGTGTCAGCATTTGCTCCAATTTGCAACCCTGTACTCTGTCCCTGGGGCAAAAAAGC CTTTAGTGGATATTTGGGAACAGATCAAAGTAAATGGAAGGCTTATGATGCTACCCACCTTGTGAAATCC TATCCAGGATCTCAGCTGGACATACTAATTGATCAAGGGAAAGATGACCAGTTTCTTTTAGATGGACAGT TACTCCCTGATAACTTCATAGCTGCCTGTACAGAAAAGAAAATCCCCGTTGTTTTTCGATTGCAAGAGGA TTATGATCATAGCTACTACTTCATTGCAACCTTTATTACTGACCACATCAGACATCATGCTAAATACCTG AATGCA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC200533 protein sequence
Red=Cloning site Green=Tags(s) MALKQISSNKCFGGLQKVFEHDSVELNCKMKFAVYLPPKAETGKCPALYWLSGLTCTEQNFISKSGYHQS ASEHGLVVIAPDTSPRGCNIKGEDESWDFGTGAGFYVDATEDPWKTNYRMYSYVTEELPQLINANFPVDP QRMSIFGHSMGGHGALICALKNPGKYKSVSAFAPICNPVLCPWGKKAFSGYLGTDQSKWKAYDATHLVKS YPGSQLDILIDQGKDDQFLLDGQLLPDNFIAACTEKKIPVVFRLQEDYDHSYYFIATFITDHIRHHAKYL NA TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001984 |
| ORF Size | 846 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_001984.1, NP_001975.1 |
| RefSeq Size | 1208 bp |
| RefSeq ORF | 849 bp |
| Locus ID | 2098 |
| UniProt ID | P10768 |
| MW | 31.5 kDa |
| Gene Summary | This gene encodes a serine hydrolase that belongs to the esterase D family. The encoded enzyme is active toward numerous substrates including O-acetylated sialic acids, and it may be involved in the recycling of sialic acids. This gene is used as a genetic marker for retinoblastoma and Wilson's disease. [provided by RefSeq, Feb 2009] |
Citations (1)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
An activity-dependent proximity ligation platform for spatially resolved quantification of active enzymes in single cells
,Li, G;Montgomery, JE;Eckert, MA;Chang, JW;Tienda, SM;Lengyel, E;Moellering, RE;,
Nat Commun
,PubMed ID 29176560
[ESD]
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Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC200533L1 | Lenti ORF clone of Human esterase D (ESD), Myc-DDK-tagged |
CNY 4,800.00 |
|
| RC200533L2 | Lenti ORF clone of Human esterase D (ESD), mGFP tagged |
CNY 5,890.00 |
|
| RC200533L3 | Lenti ORF clone of Human esterase D (ESD), Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC200533L4 | Lenti ORF clone of Human esterase D (ESD), mGFP tagged |
CNY 5,890.00 |
|
| RG200533 | ESD (tGFP-tagged) - Human esterase D (ESD) |
CNY 4,000.00 |
|
| SC310576 | ESD (untagged)-Human esterase D (ESD) |
CNY 2,400.00 |
|
| SC320664 | ESD (untagged)-Human esterase D (ESD) |
CNY 2,400.00 |
