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GADD34 (PPP1R15A) (NM_014330) Human Tagged ORF Clone
CAT＃: RC200581
PPP1R15A (Myc-DDK-tagged)-Human protein phosphatase 1, regulatory (inhibitor) subunit 15A (PPP1R15A)
ORF Plasmid: tGFP
"NM_014330" in other vectors (6)
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CNY 4,936.00
CNY 5,320.00
| Cited in 2 publications. |
Product images
CNY 300.00
CNY 1,999.00
CNY 2,700.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | GADD34 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC200581 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCCCCAGGCCAAGCACCCCATCAGGCTACCCCGTGGAGGGATGCCCACCCTTTCTTCCTCCTGTCCC CAGTGATGGGCCTCCTCAGCCGCGCCTGGAGCCGCCTGAGGGGCCTGGGACCTCTAGAGCCCTGGCTGGT GGAAGCAGTAAAAGGAGCAGCTCTGGTAGAAGCTGGCCTGGAGGGAGAAGCTAGGACTCCTCTGGCAATC CCCCATACCCCTTGGGGCAGACGCCCTGAAGAGGAGGCTGAAGACAGTGGAGGCCCTGGAGAGGACAGAG AAACACTGGGGCTGAAAACCAGCAGTTCCCTTCCTGAAGCCTGGGGACTTTTGGATGATGATGATGGCAT GTATGGTGAGCGAGAGGCAACCAGTGTCCCTAGAGGGCAGGGAAGTCAATTTGCAGATGGCCAGCGTGCT CCCCTGTCTCCCAGCCTTCTGATAAGGACACTGCAAGGTTCTGATAAGAACCCAGGGGAGGAGAAAGCCG AGGAAGAGGGAGTTGCTGAAGAGGAGGGAGTTAACAAGTTCTCTTATCCACCATCACACCGGGAGTGTTG TCCAGCCGTGGAGGAGGAGGACGATGAAGAAGCTGTAAAGAAAGAAGCTCACAGAACCTCTACTTCTGCC TTGTCTCCAGGATCCAAGCCCAGCACTTGGGTGTCTTGCCCAGGGGAGGAAGAGAATCAAGCCACGGAGG ATAAAAGAACAGAAAGAAGTAAAGGAGCCAGGAAGACCTCCGTGTCCCCCCGATCTTCAGGCTCCGACCC CAGGTCCTGGGAGTATCGTTCAGGAGAGGCGTCCGAGGAGAAGGAGGAAAAGGCACACAAAGAAACTGGG AAAGGAGAAGCTGCCCCAGGGCCGCAATCCTCAGCCCCAGCCCAGAGGCCCCAGCTCAAGTCCTGGTGGT GCCAACCCAGTGATGAAGAGGAGGGTGAGGTCAAGGCTTTGGGGGCAGCTGAGAAGGATGGAGAAGCTGA GTGTCCTCCCTGCATCCCCCCACCAAGTGCCTTCCTGAAGGCCTGGGTGTATTGGCCAGGAGAGGACACA GAGGAAGAGGAAGATGAGGAAGAAGATGAGGACAGTGACTCTGGATCAGATGAGGAAGAGGGAGAAGCTG AGGCTTCCTCTTCCACTCCTGCTACAGGTGTCTTCTTGAAGTCCTGGGTCTATCAGCCAGGAGAGGACAC AGAGGAGGAGGAAGATGAGGACAGTGATACAGGATCAGCCGAGGATGAAAGAGAAGCTGAGACTTCTGCT TCCACACCCCCTGCAAGTGCTTTCTTGAAGGCCTGGGTGTATCGGCCAGGAGAGGACACGGAGGAGGAGG AAGATGAGGATGTGGATAGTGAGGATAAGGAAGATGATTCAGAAGCAGCCTTGGGAGAAGCTGAGTCAGA CCCACATCCCTCCCACCCGGACCAGAGGGCCCACTTCAGGGGCTGGGGATATCGACCTGGAAAAGAGACA GAGGAAGAGGAAGCTGCTGAGGACTGGGGAGAAGCTGAGCCCTGCCCCTTCCGAGTGGCCATCTATGTAC CTGGAGAGAAGCCACCGCCTCCCTGGGCTCCTCCTAGGCTGCCCCTCCGACTGCAAAGGCGGCTCAAGCG CCCAGAAACCCCTACTCATGATCCGGACCCTGAGACTCCCCTAAAGGCCAGAAAGGTGCGCTTCTCCGAG AAGGTCACTGTCCATTTCCTGGCTGTCTGGGCAGGGCCGGCCCAGGCCGCCCGCCAGGGCCCCTGGGAGC AGCTTGCTCGGGATCGCAGCCGCTTCGCACGCCGCATCACCCAGGCCCAGGAGGAGCTGAGCCCCTGCCT CACCCCTGCTGCCCGGGCCAGAGCCTGGGCACGCCTCAGGAACCCACCTTTAGCCCCCATCCCTGCCCTC ACCCAGACCTTGCCTTCCTCCTCTGTCCCTTCGTCCCCAGTCCAGACCACGCCCTTGAGCCAAGCTGTGG CCACACCTTCCCGCTCGTCTGCTGCTGCAGCGGCTGCCCTGGACCTCAGTGGGAGGCGTGGC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC200581 protein sequence
Red=Cloning site Green=Tags(s) MAPGQAPHQATPWRDAHPFFLLSPVMGLLSRAWSRLRGLGPLEPWLVEAVKGAALVEAGLEGEARTPLAI PHTPWGRRPEEEAEDSGGPGEDRETLGLKTSSSLPEAWGLLDDDDGMYGEREATSVPRGQGSQFADGQRA PLSPSLLIRTLQGSDKNPGEEKAEEEGVAEEEGVNKFSYPPSHRECCPAVEEEDDEEAVKKEAHRTSTSA LSPGSKPSTWVSCPGEEENQATEDKRTERSKGARKTSVSPRSSGSDPRSWEYRSGEASEEKEEKAHKETG KGEAAPGPQSSAPAQRPQLKSWWCQPSDEEEGEVKALGAAEKDGEAECPPCIPPPSAFLKAWVYWPGEDT EEEEDEEEDEDSDSGSDEEEGEAEASSSTPATGVFLKSWVYQPGEDTEEEEDEDSDTGSAEDEREAETSA STPPASAFLKAWVYRPGEDTEEEEDEDVDSEDKEDDSEAALGEAESDPHPSHPDQRAHFRGWGYRPGKET EEEEAAEDWGEAEPCPFRVAIYVPGEKPPPPWAPPRLPLRLQRRLKRPETPTHDPDPETPLKARKVRFSE KVTVHFLAVWAGPAQAARQGPWEQLARDRSRFARRITQAQEELSPCLTPAARARAWARLRNPPLAPIPAL TQTLPSSSVPSSPVQTTPLSQAVATPSRSSAAAAAALDLSGRRG TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_014330 |
| ORF Size | 2022 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_014330.3 |
| RefSeq Size | 2399 bp |
| RefSeq ORF | 2025 bp |
| Locus ID | 23645 |
| UniProt ID | O75807 |
| Protein Families | Druggable Genome |
| MW | 73.5 kDa |
| Gene Summary | This gene is a member of a group of genes whose transcript levels are increased following stressful growth arrest conditions and treatment with DNA-damaging agents. The induction of this gene by ionizing radiation occurs in certain cell lines regardless of p53 status, and its protein response is correlated with apoptosis following ionizing radiation. [provided by RefSeq, Jul 2008] |
Citations (2)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
Activation of the eIF2α/ATF4 axis drives triple-negative breast cancer radioresistance by promoting glutathione biosynthesis
,Bai, X;Ni, J;Beretov, J;Wasinger, V;Wang, S;Zhu, Y;Graham, P;Li, Y;,
Redox Biology
,PubMed ID 33946018
[GADD34]
|
|
Integrated stress response is critical for gemcitabine resistance in pancreatic ductal adenocarcinoma
,null,
Cell Death & Disease
,PubMed ID 26469962
[GADD34]
|
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC200581L1 | Lenti ORF clone of Human protein phosphatase 1, regulatory (inhibitor) subunit 15A (PPP1R15A), Myc-DDK-tagged |
CNY 7,336.00 |
|
| RC200581L2 | Lenti ORF clone of Human protein phosphatase 1, regulatory (inhibitor) subunit 15A (PPP1R15A), mGFP tagged |
CNY 7,700.00 |
|
| RC200581L3 | Lenti ORF clone of Human protein phosphatase 1, regulatory (inhibitor) subunit 15A (PPP1R15A), Myc-DDK-tagged |
CNY 7,700.00 |
|
| RC200581L4 | Lenti ORF clone of Human protein phosphatase 1, regulatory (inhibitor) subunit 15A (PPP1R15A), mGFP tagged |
CNY 7,336.00 |
|
| RG200581 | PPP1R15A (tGFP-tagged) - Human protein phosphatase 1, regulatory (inhibitor) subunit 15A (PPP1R15A) |
CNY 6,536.00 |
|
| SC115045 | PPP1R15A (untagged)-Human protein phosphatase 1, regulatory (inhibitor) subunit 15A (PPP1R15A) |
CNY 4,944.00 |
