SERPINB6 (NM_004568) Human Tagged ORF Clone

CAT#: RC200668

SERPINB6 (Myc-DDK-tagged)-Human serpin peptidase inhibitor, clade B (ovalbumin), member 6 (SERPINB6), transcript variant 1

ORF Plasmid: DDK tGFP



  "NM_004568" in other vectors (6)

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CNY 3,656.00

CNY 5,320.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (5)
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

CNY 5,420.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00


DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 30 ul

CNY 300.00


SERPINB6 mouse monoclonal antibody, clone OTI1C5 (formerly 1C5)
    • 100 ul

CNY 1,999.00
CNY 2,700.00

Specifications

Product Data
Type Human Tagged ORF Clone
Tag Myc-DDK
Synonyms CAP; DFNB91; MSTP057; PI-6; PI6; PTI; SPI3
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RC200668 representing NM_004568
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGATGTTCTCGCAGAAGCAAATGGCACCTTTGCCTTAAACCTTTTGAAAACACTGGGTAAAGACAACT
CGAAGAATGTGTTTTTCTCACCCATGAGCATGTCCTGTGCCCTGGCCATGGTCTACATGGGGGCAAAGGG
AAACACCGCTGCACAGATGGCCCAGATACTTTCTTTCAATAAAAGTGGCGGTGGTGGAGACATCCACCAG
GGCTTCCAGTCTCTTCTCACCGAAGTGAACAAGACTGGCACGCAGTACTTGCTTAGGATGGCCAACAGGC
TCTTTGGGGAAAAGTCTTGTGATTTCCTCTCATCTTTTAGAGATTCCTGCCAAAAATTCTACCAAGCAGA
GATGGAGGAGCTTGACTTTATCAGCGCCGTAGAGAAGTCCAGAAAACACATAAACACCTGGGTAGCTGAA
AAGACAGAAGGTAAAATTGCGGAGTTGCTCTCTCCGGGCTCAGTGGATCCATTGACAAGGCTGGTTCTGG
TGAATGCTGTCTATTTCAGAGGAAACTGGGATGAACAGTTTGACAAGGAGAACACCGAGGAGAGACTGTT
TAAAGTCAGCAAGAATGAGGAGAAACCTGTGCAAATGATGTTTAAGCAATCTACTTTTAAGAAGACCTAT
ATAGGAGAAATATTTACCCAAATCTTGGTGCTTCCATATGTTGGCAAGGAACTGAATATGATCATCATGC
TTCCGGACGAGACCACTGACTTGAGAACGGTGGAGAAAGAACTCACTTACGAGAAGTTCGTAGAATGGAC
GAGGCTGGACATGATGGATGAAGAGGAGGTGGAAGTGTCCCTCCCGCGGTTTAAACTAGAGGAAAGCTAC
GACATGGAGAGTGTCCTGCGCAACCTGGGCATGACTGATGCCTTCGAGCTGGGCAAGGCAGACTTCTCTG
GAATGTCCCAGACAGACCTGTCTCTGTCCAAGGTCGTGCACAAGTCTTTTGTGGAGGTCAATGAGGAAGG
CACGGAGGCTGCAGCCGCCACAGCTGCCATCATGATGATGCGGTGTGCCAGATTCGTCCCCCGCTTCTGC
GCCGACCACCCCTTCCTTTTCTTCATCCAGCACAGCAAGACCAACGGGATTCTCTTCTGCGGCCGCTTTT
CCTCTCCG


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RC200668 representing NM_004568
Red=Cloning site Green=Tags(s)

MDVLAEANGTFALNLLKTLGKDNSKNVFFSPMSMSCALAMVYMGAKGNTAAQMAQILSFNKSGGGGDIHQ
GFQSLLTEVNKTGTQYLLRMANRLFGEKSCDFLSSFRDSCQKFYQAEMEELDFISAVEKSRKHINTWVAE
KTEGKIAELLSPGSVDPLTRLVLVNAVYFRGNWDEQFDKENTEERLFKVSKNEEKPVQMMFKQSTFKKTY
IGEIFTQILVLPYVGKELNMIIMLPDETTDLRTVEKELTYEKFVEWTRLDMMDEEEVEVSLPRFKLEESY
DMESVLRNLGMTDAFELGKADFSGMSQTDLSLSKVVHKSFVEVNEEGTEAAAATAAIMMMRCARFVPRFC
ADHPFLFFIQHSKTNGILFCGRFSSP

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_004568
ORF Size 1128 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_004568.6
RefSeq Size 1665 bp
RefSeq ORF 1131 bp
Locus ID 5269
UniProt ID P35237
Domains SERPIN
Protein Families Druggable Genome
MW 42.4 kDa
Gene Summary The protein encoded by this gene is a member of the serpin (serine proteinase inhibitor) superfamily, and ovalbumin(ov)-serpin subfamily. It was originally discovered as a placental thrombin inhibitor. The mouse homolog was found to be expressed in the hair cells of the inner ear. Mutations in this gene are associated with nonsyndromic progressive hearing loss, suggesting that this serpin plays an important role in the inner ear in the protection against leakage of lysosomal content during stress, and that loss of this protection results in cell death and sensorineural hearing loss. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Sep 2010]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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