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SOX2 (NM_003106) Human Tagged ORF Clone
CAT＃: RC200757
SOX2 (Myc-DDK-tagged)-Human SRY (sex determining region Y)-box 2 (SOX2)
ORF Plasmid: tGFP
"NM_003106" in other vectors (6)
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CNY 3,600.00
CNY 4,465.00
| Cited in 7 publications. |
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | ANOP3; MCOPS3 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC200757 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTACAACATGATGGAGACGGAGCTGAAGCCGCCGGGCCCGCAGCAAACTTCGGGGGGCGGCGGCGGCA ACTCCACCGCGGCGGCGGCCGGCGGCAACCAGAAAAACAGCCCGGACCGCGTCAAGCGGCCCATGAATGC CTTCATGGTGTGGTCCCGCGGGCAGCGGCGCAAGATGGCCCAGGAGAACCCCAAGATGCACAACTCGGAG ATCAGCAAGCGCCTGGGCGCCGAGTGGAAACTTTTGTCGGAGACGGAGAAGCGGCCGTTCATCGACGAGG CTAAGCGGCTGCGAGCGCTGCACATGAAGGAGCACCCGGATTATAAATACCGGCCCCGGCGGAAAACCAA GACGCTCATGAAGAAGGATAAGTACACGCTGCCCGGCGGGCTGCTGGCCCCCGGCGGCAATAGCATGGCG AGCGGGGTCGGGGTGGGCGCCGGCCTGGGCGCGGGCGTGAACCAGCGCATGGACAGTTACGCGCACATGA ACGGCTGGAGCAACGGCAGCTACAGCATGATGCAGGACCAGCTGGGCTACCCGCAGCACCCGGGCCTCAA TGCGCACGGCGCAGCGCAGATGCAGCCCATGCACCGCTACGACGTGAGCGCCCTGCAGTACAACTCCATG ACCAGCTCGCAGACCTACATGAACGGCTCGCCCACCTACAGCATGTCCTACTCGCAGCAGGGCACCCCTG GCATGGCTCTTGGCTCCATGGGTTCGGTGGTCAAGTCCGAGGCCAGCTCCAGCCCCCCTGTGGTTACCTC TTCCTCCCACTCCAGGGCGCCCTGCCAGGCCGGGGACCTCCGGGACATGATCAGCATGTATCTCCCCGGC GCCGAGGTGCCGGAACCCGCCGCCCCCAGCAGACTTCACATGTCCCAGCACTACCAGAGCGGCCCGGTGC CCGGCACGGCCATTAACGGCACACTGCCCCTCTCACACATG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC200757 protein sequence
Red=Cloning site Green=Tags(s) MYNMMETELKPPGPQQTSGGGGGNSTAAAAGGNQKNSPDRVKRPMNAFMVWSRGQRRKMAQENPKMHNSE ISKRLGAEWKLLSETEKRPFIDEAKRLRALHMKEHPDYKYRPRRKTKTLMKKDKYTLPGGLLAPGGNSMA SGVGVGAGLGAGVNQRMDSYAHMNGWSNGSYSMMQDQLGYPQHPGLNAHGAAQMQPMHRYDVSALQYNSM TSSQTYMNGSPTYSMSYSQQGTPGMALGSMGSVVKSEASSSPPVVTSSSHSRAPCQAGDLRDMISMYLPG AEVPEPAAPSRLHMSQHYQSGPVPGTAINGTLPLSHM TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_003106 |
| ORF Size | 951 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_003106.4 |
| RefSeq Size | 2520 bp |
| RefSeq ORF | 954 bp |
| Locus ID | 6657 |
| UniProt ID | P48431 |
| Protein Families | Adult stem cells, Cancer stem cells, Embryonic stem cells, ES Cell Differentiation/IPS, Induced pluripotent stem cells, Transcription Factors |
| MW | 34.3 kDa |
| Gene Summary | This intronless gene encodes a member of the SRY-related HMG-box (SOX) family of transcription factors involved in the regulation of embryonic development and in the determination of cell fate. The product of this gene is required for stem-cell maintenance in the central nervous system, and also regulates gene expression in the stomach. Mutations in this gene have been associated with optic nerve hypoplasia and with syndromic microphthalmia, a severe form of structural eye malformation. This gene lies within an intron of another gene called SOX2 overlapping transcript (SOX2OT). [provided by RefSeq, Jul 2008] |
Citations (7)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
SOX9 and TCF transcription factors associate to mediate Wnt/β-catenin target gene activation in colorectal cancer
,null,
The Journal of Biological Chemistry
,PubMed ID 36423688
[SOX2]
|
|
Downregulation of SOX2 by inhibition of Usp9X induces apoptosis in melanoma
,Potu, H;Kandarpa, M;Peterson, LF;Durham, A;Donato, NJ;Talpaz, M;,
Oncotarget
,PubMed ID 33613844
[SOX2]
|
|
FOLFOX Therapy Induces Feedback Upregulation of CD44v6 through YB-1 to Maintain Stemness in Colon Initiating Cells
,Ghatak, S;Hascall, VC;Markwald, RR;Misra, S;,
International journal of molecular sciences
,PubMed ID 33451103
[SOX2]
|
|
Downregulation of FOXO3a by DNMT1 promotes breast cancer stem cell properties and tumorigenesis
,Liu, H;Song, Y;Qiu, H;Liu, Y;Luo, K;Yi, Y;Jiang, G;Lu, M;Zhang, Z;Yin, J;Zeng, S;Chen, X;Deng, M;Jia, X;Gu, Y;Chen, D;Zheng, G;He, Z;,
Cell Death Differ.
,PubMed ID 31296961
[SOX2]
|
|
O-GlcNAc modification of Sox2 regulates self-renewal in pancreatic cancer by promoting its stability
,null,
Theranostics
,PubMed ID 31281487
[SOX2]
|
|
Oncogenic Sox2 regulates and cooperates with VRK1 in cell cycle progression and differentiation
,Moura, DS;Fernández, IF;Marín-Royo, G;López-Sánchez, I;Martín-Doncel, E;Vega, FM;Lazo, PA;,
Sci Rep
,PubMed ID 27334688
[SOX2]
|
|
Loss of miR-638 in vitro promotes cell invasion and a mesenchymal-like transition by influencing SOX2 expression in colorectal carcinoma cells
,Ma, K;Pan, X;Fan, P;He, Y;Gu, J;Wang, W;Zhang, T;Li, Z;Luo, X;,
Mol. Cancer
,PubMed ID 24885288
[SOX2]
|
Documents
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| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC200757L1 | Lenti ORF clone of Human SRY (sex determining region Y)-box 2 (SOX2), Myc-DDK-tagged |
CNY 6,000.00 |
|
| RC200757L2 | Lenti ORF clone of Human SRY (sex determining region Y)-box 2 (SOX2), mGFP tagged |
CNY 6,000.00 |
|
| RC200757L3 | Lenti ORF clone of Human SRY (sex determining region Y)-box 2 (SOX2), Myc-DDK-tagged |
CNY 6,000.00 |
|
| RC200757L4 | Lenti ORF clone of Human SRY (sex determining region Y)-box 2 (SOX2), mGFP tagged |
CNY 6,000.00 |
|
| RG200757 | SOX2 (tGFP-tagged) - Human SRY (sex determining region Y)-box 2 (SOX2) |
CNY 5,200.00 |
|
| SC303274 | SOX2 (untagged)-Human SRY (sex determining region Y)-box 2 (SOX2) |
CNY 3,600.00 |
