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TTLL12 (NM_015140) Human Tagged ORF Clone
CAT＃: RC200841
TTLL12 (Myc-DDK-tagged)-Human tubulin tyrosine ligase-like family, member 12 (TTLL12)
ORF Plasmid: tGFP
"NM_015140" in other vectors (6)
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CNY 4,792.00
Product images
CNY 300.00
CNY 1,999.00
CNY 2,700.00
热销推荐Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | dJ526I14.2 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC200841 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGGCCGAGCGGGGTCCCGAGCGCCGGCCTGCGGAGCGTAGCAGCCCGGGCCAGACGCCGGAGGAGG GCGCGCAGGCCTTGGCCGAGTTCGCGGCGCTGCACGGCCCGGCGCTGCGCGCTTCGGGGGTCCCCGAACG TTACTGGGGCCGCCTCCTGCACAAGCTGGAGCACGAGGTTTTCGACGCTGGGGAAGTGTTTGGGATCATG CAAGTGGAGGAGGTAGAAGAGGAGGAGGACGAGGCAGCCCGGGAGGTGCGGAAGCAGCAGCCCAACCCGG GGAACGAGCTGTGCTACAAGGTCATCGTGACCAGGGAGAGCGGGCTCCAGGCAGCCCACCCCAACAGCAT CTTCCTCATCGACCACGCCTGGACGTGCCGTGTGGAGCACGCGCGCCAGCAGCTGCAGCAGGTGCCCGGG CTGCTGCACCGCATGGCCAACCTGATGGGCATTGAGTTCCACGGTGAGCTGCCCAGTACAGAGGCTGTGG CCCTGGTGCTGGAGGAGATGTGGAAGTTCAACCAGACCTACCAGCTGGCCCATGGGACAGCTGAGGAGAA GATGCCGGTGTGGTATATCATGGACGAGTTCGGTTCGCGGATCCAGCACGCGGACGTGCCCAGCTTCGCC ACGGCACCCTTCTTCTACATGCCGCAGCAGGTGGCCTACACGCTGCTGTGGCCCCTGAGGGACCTGGACA CTGGCGAGGAGGTGACCCGAGACTTTGCCTACGGAGAGACGGACCCCCTGATCCGGAAGTGCATGCTGCT GCCCTGGGCCCCCACCGACATGCTGGACCTCAGCTCTTGCACACCCGAGCCGCCCGCCGAGCACTACCAG GCCATTCTGGAGGAAAACAAGGAGAAGCTGCCACTTGACATCAACCCCGTGGTGCACCCCCACGGCCACA TCTTCAAGGTCTACACGGACGTGCAGCAGGTGGCCAGCAGCCTCACCCACCCGCGCTTCACCCTCACCCA GAGTGAGGCGGACGCCGACATCCTCTTCAACTTCTCACACTTCAAGGACTACAGGAAACTCAGCCAGGAG AGGCCAGGCGTGCTGCTGAACCAGTTCCCCTGCGAGAACCTGCTGACTGTCAAGGACTGCCTGGCCTCCA TCGCGCGCCGGGCAGGTGGCCCCGAGGGCCCACCCTGGCTGCCCCGAACCTTCAACCTGCGCACTGAGCT GCCCCAGTTTGTCAGCTACTTCCAGCAGCGGGAAAGGTGGGGCGAGGACAACCACTGGATCTGCAAGCCC TGGAACCTGGCGCGCAGCCTGGACACCCACGTCACCAAGAGCCTGCACAGCATCATCCGGCACCGAGAGA GCACCCCCAAGGTTGTGTCCAAGTACATCGAAAGTCCCGTGTTGTTCCTTCGAGAAGACGTGGGAAAGGT CAAGTTCGACATCCGCTACATCGTGCTGCTGCGGTCAGTGAGGCCCCTACGGTTGTTCGTGTATGATGTG TTCTGGCTGCGGTTCTCCAACCGGGCCTTTGCACTCAACGACCTGGATGACTACGAGAAGCACTTCACGG TCATGAACTATGACCCGGATGTGGTGCTGAAGCAGGTGCACTGTGAAGAGTTCATCCCCGAGTTTGAGAA GCAATACCCAGAATTTCCCTGGACGGACGTCCAGGCTGAGATCTTCCGGGCCTTCACGGAGCTGTTCCAG GTGGCCTGTGCCAAGCCACCACCCCTGGGCCTCTGCGACTACCCCTCATCCCGGGCCATGTATGCCGTCG ACCTCATGCTGAAGTGGGACAACGGCCCAGATGGAAGGCGGGTGATGCAGCCGCAGATCCTGGAGGTGAA CTTCAACCCCGACTGTGAGCGAGCCTGCAGGTACCACCCCACCTTCTTCAACGACGTCTTCAGCACCTTG TTTCTGGACCAGCCCGGTGGCTGCCACGTTACCTGCCTTGTC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC200841 protein sequence
Red=Cloning site Green=Tags(s) MEAERGPERRPAERSSPGQTPEEGAQALAEFAALHGPALRASGVPERYWGRLLHKLEHEVFDAGEVFGIM QVEEVEEEEDEAAREVRKQQPNPGNELCYKVIVTRESGLQAAHPNSIFLIDHAWTCRVEHARQQLQQVPG LLHRMANLMGIEFHGELPSTEAVALVLEEMWKFNQTYQLAHGTAEEKMPVWYIMDEFGSRIQHADVPSFA TAPFFYMPQQVAYTLLWPLRDLDTGEEVTRDFAYGETDPLIRKCMLLPWAPTDMLDLSSCTPEPPAEHYQ AILEENKEKLPLDINPVVHPHGHIFKVYTDVQQVASSLTHPRFTLTQSEADADILFNFSHFKDYRKLSQE RPGVLLNQFPCENLLTVKDCLASIARRAGGPEGPPWLPRTFNLRTELPQFVSYFQQRERWGEDNHWICKP WNLARSLDTHVTKSLHSIIRHRESTPKVVSKYIESPVLFLREDVGKVKFDIRYIVLLRSVRPLRLFVYDV FWLRFSNRAFALNDLDDYEKHFTVMNYDPDVVLKQVHCEEFIPEFEKQYPEFPWTDVQAEIFRAFTELFQ VACAKPPPLGLCDYPSSRAMYAVDLMLKWDNGPDGRRVMQPQILEVNFNPDCERACRYHPTFFNDVFSTL FLDQPGGCHVTCLV TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_015140 |
| ORF Size | 1932 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_015140.4 |
| RefSeq Size | 3421 bp |
| RefSeq ORF | 1935 bp |
| Locus ID | 23170 |
| UniProt ID | Q14166 |
| MW | 74.4 kDa |
| Gene Summary | Negatively regulates post-translational modifications of tubulin, including detyrosination of the C-terminus and polyglutamylation of glutamate residues (PubMed:20162578, PubMed:23251473). Also, indirectly promotes histone H4 trimethylation at 'Lys-20' (H4K20me3) (PubMed:23251473). Probably by controlling tubulin and/or histone H4 post-translational modifications, plays a role in mitosis and in maintaining chromosome number stability (PubMed:20162578, PubMed:23251473). During RNA virus-mediated infection, acts as a negative regulator of the DDX58/RIG-I pathway by preventing MAVS binding to TBK1 and IKBKE (PubMed:28011935).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC200841L1 | Lenti ORF clone of Human tubulin tyrosine ligase-like family, member 12 (TTLL12), Myc-DDK-tagged |
CNY 7,192.00 |
|
| RC200841L2 | Lenti ORF clone of Human tubulin tyrosine ligase-like family, member 12 (TTLL12), mGFP tagged |
CNY 5,890.00 |
|
| RC200841L3 | Lenti ORF clone of Human tubulin tyrosine ligase-like family, member 12 (TTLL12), Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC200841L4 | Lenti ORF clone of Human tubulin tyrosine ligase-like family, member 12 (TTLL12), mGFP tagged |
CNY 5,890.00 |
|
| RG200841 | TTLL12 (tGFP-tagged) - Human tubulin tyrosine ligase-like family, member 12 (TTLL12) |
CNY 6,392.00 |
|
| SC122822 | TTLL12 (untagged)-Human tubulin tyrosine ligase-like family, member 12 (TTLL12) |
CNY 4,800.00 |
