Zyxin (ZYX) (NM_001010972) Human Tagged ORF Clone
CAT#: RC200960
- TrueORF®
ZYX (Myc-DDK-tagged)-Human zyxin (ZYX), transcript variant 2
ORF Plasmid: tGFP
"NM_001010972" in other vectors (4)
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CNY 4,256.00
CNY 300.00
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | ESP-2; HED-2 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RC200960 representing NM_001010972
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGGCCCCCCGCCCGTCTCCCGCGATCTCCGTTTCGGTCTCGGCTCCGGCTTTTTACGCCCCGCAGA AGAAGTTCGGCCCTGTGGTGGCCCCAAAGCCCAAAGTGAATCCCTTCCGGCCCGGGGACAGCGAGCCTCC CCCGGCACCCGGGGCCCAGCGCGCACAGATGGGCCGGGTGGGCGAGATTCCCCCGCCGCCCCCGGAAGAC TTTCCCCTGCCTCCACCTCCCCTTGCTGGGGATGGCGACGATGCAGAGGGTGCTCTGGGAGGTGCCTTCC CGCCGCCCCCTCCCCCGATCGAGGAATCATTTCCCCCTGCGCCTCTGGAGGAGGAGATCTTCCCTTCCCC GCCGCCTCCTCCGGAGGAGGAGGGAGGGCCTGAGGCCCCCATACCGCCCCCACCACAGCCCAGGGAGAAG GTGAGCAGTATTGATTTGGAGATCGACTCTCTGTCCTCACTGCTGGATGACATGACCAAGAATGATCCTT TCAAAGCCCGGGTGTCATCTGGATATGTGCCCCCACCAGTGGCCACTCCATTCAGTTCCAAGTCCAGTAC CAAGCCTGCAGCCGGGGGCACAGCACCCCTGCCTCCTTGGAAGTCCCCTTCCAGCTCCCAGCCTCTGCCC CAGGTTCCGGCTCCGGCTCAGAGCCAGACACAGTTCCATGTTCAGCCCCAGCCCCAGCCCAAGCCTCAGG TCCAACTCCATGTCCAGTCCCAGACCCAGCCTGTGTCTTTGGCTAACACCCAGCCCCGAGGGCCCCCAGC CTCATCTCCGGCTCCAGCCCCTAAGTTTTCTCCAGTGACTCCTAAGTTTACTCCTGTGGCTTCCAAGTTC AGTCCTGGAGCCCCAGGTGGATCTGGGTCACAACCAAATCAAAAATTGGGGCACCCCGAAGCTCTTTCTG CTGGCACAGGCTCCCCTCAACCTCCCAGCTTCACCTATGCCCAGCAGAGGGAGAAGCCCCGAGTGCAGGA GAAGCAGCACCCCGTGCCCCCACCGGCTCAGAACCAAAACCAGGTGCGCTCCCCTGGGGCCCCAGGGCCC CTGACTCTGAAGGAGGTGGAGGAGCTGGAGCAGCTGACCCAGCAGCTAATGCAGGACATGGAGCATCCTC AGAGGCAGAATGTGGCTGTCAACGAACTCTGCGGCCGATGCCATCAACCCCTGGCCCGGGCGCAGCCAGC CGTCCGCGCTCTAGGGCAGCTGTTCCACATCGCCTGCTTCACCTGCCACCAGTGTGCGCAGCAGCTCCAG GGCCAGCAGTTCTACAGTCTGGAGGGGGCGCCGTACTGCGAGGGCTGTTACACTGACACCCTGGAGAAGT GTAACACCTGCGGGGAGCCCATCACTGACCGCATGCTGAGGGCCACGGGCAAGGCCTATCACCCGCACTG CTTCACCTGTGTGGTCTGCGCCCGCCCCCTGGAGGGCACCTCCTTCATCGTGGACCAGGCCAACCGGCCC CACTGTGTCCCCGACTACCACAAGCAGTACGCCCCGAGGTGCTCCGTCTGCTCTGAGCCCATCATGCCTG AGCCTGGCCGAGATGAGACTGTGCGAGTGGTCGCCCTGGACAAGAACTTCCACATGAAGTGTTACAAGTG TGAGGACTGCGGGAAGCCCCTGTCGATTGAGGCAGATGACAATGGCTGCTTCCCCCTGGACGGTCACGTG CTCTGTCGGAAGTGCCACACTGCTAGAGCCCAGACC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC200960 representing NM_001010972
Red=Cloning site Green=Tags(s) MAAPRPSPAISVSVSAPAFYAPQKKFGPVVAPKPKVNPFRPGDSEPPPAPGAQRAQMGRVGEIPPPPPED FPLPPPPLAGDGDDAEGALGGAFPPPPPPIEESFPPAPLEEEIFPSPPPPPEEEGGPEAPIPPPPQPREK VSSIDLEIDSLSSLLDDMTKNDPFKARVSSGYVPPPVATPFSSKSSTKPAAGGTAPLPPWKSPSSSQPLP QVPAPAQSQTQFHVQPQPQPKPQVQLHVQSQTQPVSLANTQPRGPPASSPAPAPKFSPVTPKFTPVASKF SPGAPGGSGSQPNQKLGHPEALSAGTGSPQPPSFTYAQQREKPRVQEKQHPVPPPAQNQNQVRSPGAPGP LTLKEVEELEQLTQQLMQDMEHPQRQNVAVNELCGRCHQPLARAQPAVRALGQLFHIACFTCHQCAQQLQ GQQFYSLEGAPYCEGCYTDTLEKCNTCGEPITDRMLRATGKAYHPHCFTCVVCARPLEGTSFIVDQANRP HCVPDYHKQYAPRCSVCSEPIMPEPGRDETVRVVALDKNFHMKCYKCEDCGKPLSIEADDNGCFPLDGHV LCRKCHTARAQT TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
![]() Sequencher program is needed, download here. |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_001010972 |
ORF Size | 1716 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001010972.2 |
RefSeq Size | 2322 bp |
RefSeq ORF | 1719 bp |
Locus ID | 7791 |
UniProt ID | Q15942 |
Protein Pathways | Focal adhesion |
MW | 61.3 kDa |
Gene Summary | Focal adhesions are actin-rich structures that enable cells to adhere to the extracellular matrix and at which protein complexes involved in signal transduction assemble. Zyxin is a zinc-binding phosphoprotein that concentrates at focal adhesions and along the actin cytoskeleton. Zyxin has an N-terminal proline-rich domain and three LIM domains in its C-terminal half. The proline-rich domain may interact with SH3 domains of proteins involved in signal transduction pathways while the LIM domains are likely involved in protein-protein binding. Zyxin may function as a messenger in the signal transduction pathway that mediates adhesion-stimulated changes in gene expression and may modulate the cytoskeletal organization of actin bundles. Alternative splicing results in multiple transcript variants that encode the same isoform. [provided by RefSeq, Jul 2008] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC200960L3 | Lenti-ORF clone of ZYX (Myc-DDK-tagged)-Human zyxin (ZYX), transcript variant 2 |
CNY 5,890.00 |
|
RC200960L4 | Lenti-ORF clone of ZYX (mGFP-tagged)-Human zyxin (ZYX), transcript variant 2 |
CNY 5,890.00 |
|
RG200960 | ZYX (tGFP-tagged) - Human zyxin (ZYX), transcript variant 2 |
CNY 4,370.00 |
|
SC319514 | ZYX (untagged)-Human zyxin (ZYX), transcript variant 2 |
CNY 4,264.00 |