DCTN5 (NM_032486) Human Tagged ORF Clone
CAT#: RC201004
DCTN5 (Myc-DDK-tagged)-Human dynactin 5 (p25) (DCTN5), transcript variant 1
ORF Plasmid: tGFP
"NM_032486" in other vectors (4)
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CNY 2,400.00
CNY 3,705.00
CNY 300.00
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RC201004 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGTTGGGCGAGCTGCTCTACAACAAGTCTGAGTACATCGAGACGGCATCTGGGAACAAAGTCAGTC GCCAGTCAGTGTTGTGTGGAAGCCAGAACATCGTTCTCAATGGCAAGACCATTGTGATGAATGACTGTAT TATCCGAGGGGATCTGGCAAATGTAAGAGTTGGACGTCATTGTGTTGTGAAAAGTCGTAGTGTCATAAGG CCACCATTCAAGAAGTTCAGCAAAGGTGTTGCATTCTTTCCTTTACATATTGGAGACCATGTCTTTATTG AGGAAGATTGTGTGGTCAACGCAGCACAGATTGGTTCCTATGTTCATGTTGGGAAGAACTGTGTGATTGG GCGCCGATGTGTGTTGAAAGACTGCTGCAAAATTCTTGACAACACAGTATTACCTCCAGAAACTGTGGTT CCACCATTCACTGTCTTCTCAGGCTGCCCAGGACTCTTCTCAGGGGAGCTCCCGGAGTGCACTCAGGAGC TGATGATTGACGTCACCAAGAGCTACTACCAGAAGTTTTTGCCCCTGACGCAAGTC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC201004 protein sequence
Red=Cloning site Green=Tags(s) MELGELLYNKSEYIETASGNKVSRQSVLCGSQNIVLNGKTIVMNDCIIRGDLANVRVGRHCVVKSRSVIR PPFKKFSKGVAFFPLHIGDHVFIEEDCVVNAAQIGSYVHVGKNCVIGRRCVLKDCCKILDNTVLPPETVV PPFTVFSGCPGLFSGELPECTQELMIDVTKSYYQKFLPLTQV TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
Sequencher program is needed, download here. |
Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
ACCN | NM_032486 |
ORF Size | 546 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_032486.4 |
RefSeq Size | 7329 bp |
RefSeq ORF | 549 bp |
Locus ID | 84516 |
UniProt ID | Q9BTE1 |
Domains | hexapep |
MW | 20.1 kDa |
Gene Summary | This gene encodes a subunit of dynactin, a component of the cytoplasmic dynein motor machinery involved in minus-end-directed transport. The encoded protein is a component of the pointed-end subcomplex and is thought to bind membranous cargo. A pseudogene of this gene is located on the long arm of chromosome 1. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Jan 2011] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC201004L3 | Lenti ORF clone of Human dynactin 5 (p25) (DCTN5), transcript variant 1, Myc-DDK-tagged |
CNY 5,890.00 |
|
RC201004L4 | Lenti ORF clone of Human dynactin 5 (p25) (DCTN5), transcript variant 1, mGFP tagged |
CNY 5,890.00 |
|
RG201004 | DCTN5 (tGFP-tagged) - Human dynactin 5 (p25) (DCTN5), transcript variant 1 |
CNY 4,000.00 |
|
SC104880 | DCTN5 (untagged)-Human dynactin 5 (p25) (DCTN5), transcript variant 1 |
CNY 3,990.00 |