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NSFL1C (NM_016143) Human Tagged ORF Clone
CAT＃: RC201022
NSFL1C (Myc-DDK-tagged)-Human NSFL1 (p97) cofactor (p47) (NSFL1C), transcript variant 1
ORF Plasmid: tGFP
"NM_016143" in other vectors (6)
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CNY 3,656.00
CNY 3,990.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | dJ776F14.1; P47; UBX1; UBXD10; UBXN2C |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC201022 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGGCGGAGCGACAGGAGGCGCTGAGGGAGTTCGTGGCGGTGACGGGCGCCGAGGAGGACCGGGCCC GCTTCTTTCTCGAGTCGGCCGGCTGGGACTTGCAGATCGCGCTAGCGAGCTTTTATGAGGACGGAGGGGA TGAAGACATTGTGACCATTTCGCAGGCAACCCCCAGTTCAGTGTCCAGAGGCACAGCCCCCAGTGATAAT AGAGTGACATCCTTCAGAGACCTCATTCATGACCAAGATGAAGATGAGGAGGAAGAGGAAGGCCAGAGGT TTTATGCTGGGGGCTCAGAGAGAAGTGGACAGCAGATTGTTGGCCCTCCCAGGAAGAAAAGTCCCAACGA GCTGGTGGATGATCTCTTTAAAGGTGCCAAAGAGCATGGAGCTGTAGCTGTGGAGCGAGTGACCAAGAGC CCTGGAGAGACCAGTAAACCGAGACCATTTGCAGGAGGTGGCTACCGCCTTGGGGCAGCACCAGAGGAAG AGTCTGCCTATGTGGCAGGAGAAAAGAGGCAGCATTCCAGCCAAGATGTTCATGTAGTATTGAAACTCTG GAAGAGTGGATTCAGCCTGGATAATGGAGAACTCAGAAGCTACCAAGACCCATCCAATGCCCAGTTTCTG GAGTCTATCCGCAGAGGGGAGGTGCCAGCAGAGCTTCGGAGGCTAGCTCACGGTGGACAGGTGAACTTGG ATATGGAGGACCATCGGGACGAGGACTTTGTGAAGCCCAAAGGAGCCTTCAAAGCCTTCACTGGCGAGGG TCAGAAACTGGGCAGCACTGCCCCCCAGGTGTTGAGTACCAGCTCTCCAGCCCAACAGGCAGAAAATGAA GCCAAAGCCAGCTCTTCCATCTTAATCGACGAATCAGAGCCTACCACAAACATCCAAATTCGGCTTGCAG ACGGCGGGAGGCTGGTGCAGAAATTTAACCACAGCCACAGGATCAGCGACATCCGACTCTTCATCGTGGA TGCCCGGCCAGCCATGGCTGCCACCAGCTTTATCCTCATGACTACTTTCCCGAACAAAGAGCTGGCTGAT GAGAGCCAGACCCTGAAGGAAGCCAACCTGCTCAATGCTGTCATCGTGCAGCGGTTAACA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC201022 protein sequence
Red=Cloning site Green=Tags(s) MAAERQEALREFVAVTGAEEDRARFFLESAGWDLQIALASFYEDGGDEDIVTISQATPSSVSRGTAPSDN RVTSFRDLIHDQDEDEEEEEGQRFYAGGSERSGQQIVGPPRKKSPNELVDDLFKGAKEHGAVAVERVTKS PGETSKPRPFAGGGYRLGAAPEEESAYVAGEKRQHSSQDVHVVLKLWKSGFSLDNGELRSYQDPSNAQFL ESIRRGEVPAELRRLAHGGQVNLDMEDHRDEDFVKPKGAFKAFTGEGQKLGSTAPQVLSTSSPAQQAENE AKASSSILIDESEPTTNIQIRLADGGRLVQKFNHSHRISDIRLFIVDARPAMAATSFILMTTFPNKELAD ESQTLKEANLLNAVIVQRLT TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_016143 |
| ORF Size | 1110 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_016143.4 |
| RefSeq Size | 3568 bp |
| RefSeq ORF | 1113 bp |
| Locus ID | 55968 |
| UniProt ID | Q9UNZ2 |
| Domains | UBX, FAF |
| MW | 40.6 kDa |
| Gene Summary | N-ethylmaleimide-sensitive factor (NSF) and valosin-containing protein (p97) are two ATPases known to be involved in transport vesicle/target membrane fusion and fusions between membrane compartments. A trimer of the protein encoded by this gene binds a hexamer of cytosolic p97 and is required for p97-mediated regrowth of Golgi cisternae from mitotic Golgi fragments. Alternative splicing results in multiple transcript variants. A related pseudogene has been identified on chromosome 8. [provided by RefSeq, May 2011] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC201022L1 | Lenti ORF clone of Human NSFL1 (p97) cofactor (p47) (NSFL1C), transcript variant 1, Myc-DDK-tagged |
CNY 6,056.00 |
|
| RC201022L2 | Lenti ORF clone of Human NSFL1 (p97) cofactor (p47) (NSFL1C), transcript variant 1, mGFP tagged |
CNY 5,890.00 |
|
| RC201022L3 | Lenti ORF clone of Human NSFL1 (p97) cofactor (p47) (NSFL1C), transcript variant 1, Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC201022L4 | Lenti ORF clone of Human NSFL1 (p97) cofactor (p47) (NSFL1C), transcript variant 1, mGFP tagged |
CNY 5,890.00 |
|
| RG201022 | NSFL1C (tGFP-tagged) - Human NSFL1 (p97) cofactor (p47) (NSFL1C), transcript variant 1 |
CNY 5,256.00 |
|
| SC127944 | NSFL1C (untagged)-Human NSFL1 (p97) cofactor (p47) (NSFL1C), transcript variant 1 |
CNY 3,656.00 |
