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STIP1 (NM_006819) Human Tagged ORF Clone
CAT＃: RC201084
STIP1 (Myc-DDK-tagged)-Human stress-induced-phosphoprotein 1 (STIP1)
ORF Plasmid: tGFP
"NM_006819" in other vectors (6)
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CNY 4,040.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | HEL-S-94n; HOP; IEF-SSP-3521; P60; STI1; STI1L |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC201084 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGCAGGTCAATGAGCTGAAGGAGAAAGGCAACAAGGCCCTGAGCGTGGGTAACATCGATGATGCCT TACAGTGCTACTCCGAAGCTATTAAGCTGGATCCCCACAACCACGTGCTGTACAGCAACCGTTCTGCTGC CTATGCCAAGAAAGGAGACTACCAGAAGGCTTATGAGGATGGCTGCAAGACTGTCGACCTAAAGCCTGAC TGGGGCAAGGGCTATTCACGAAAAGCAGCAGCTCTAGAGTTCTTAAACCGCTTTGAAGAAGCCAAGCGAA CCTATGAGGAGGGCTTAAAACACGAGGCAAATAACCCTCAACTGAAAGAGGGTTTACAGAATATGGAGGC CAGGTTGGCAGAGAGAAAATTCATGAACCCTTTCAACATGCCTAATCTGTATCAGAAGTTGGAGAGTGAT CCCAGGACAAGGACACTACTCAGTGATCCTACCTACCGGGAGCTGATAGAGCAGCTACGAAACAAGCCTT CTGACCTGGGCACGAAACTACAAGATCCCCGGATCATGACCACTCTCAGCGTCCTCCTTGGGGTCGATCT GGGCAGTATGGATGAGGAGGAAGAGATTGCAACACCTCCACCACCACCCCCTCCCAAAAAGGAGACCAAG CCAGAGCCAATGGAAGAAGATCTTCCAGAGAATAAGAAGCAGGCACTGAAAGAAAAAGAGCTGGGGAACG ATGCCTACAAGAAGAAAGACTTTGACACAGCCTTGAAGCATTACGACAAAGCCAAGGAGCTGGACCCCAC TAACATGACTTACATTACCAATCAAGCAGCGGTATACTTTGAAAAGGGCGACTACAATAAGTGCCGGGAG CTTTGTGAGAAGGCCATTGAAGTGGGGAGAGAAAACCGAGAAGACTATCGACAGATTGCCAAAGCATATG CTCGAATTGGCAACTCCTACTTCAAAGAAGAAAAGTACAAGGATGCCATCCATTTCTATAACAAGTCTCT GGCAGAGCACCGAACCCCAGATGTGCTCAAGAAATGCCAGCAGGCAGAGAAAATCCTGAAGGAGCAAGAG CGGCTGGCCTACATAAACCCCGACCTGGCTTTGGAGGAGAAGAACAAAGGCAACGAGTGTTTTCAGAAAG GGGACTATCCCCAGGCCATGAAGCATTATACAGAAGCCATCAAAAGGAACCCGAAAGATGCCAAATTATA CAGCAATCGAGCTGCCTGCTACACCAAACTCCTGGAGTTCCAGCTGGCACTCAAGGACTGTGAGGAATGT ATCCAGCTGGAGCCGACCTTCATCAAGGGTTATACACGGAAAGCCGCTGCGCTGGAAGCGATGAAGGACT ACACCAAAGCCATGGATGTGTACCAGAAGGCGCTAGACCTGGACTCCAGCTGTAAGGAGGCGGCAGACGG CTACCAGCGCTGTATGATGGCGCAGTACAACCGGCACGACAGCCCCGAAGATGTGAAGCGACGAGCCATG GCCGACCCTGAGGTGCAGCAGATCATGAGTGACCCAGCCATGCGCCTTATCCTGGAACAGATGCAGAAGG ACCCCCAGGCACTCAGCGAACACTTAAAGAATCCTGTAATAGCACAGAAGATCCAGAAGCTGATGGATGT GGGTCTGATTGCAATTCGG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC201084 protein sequence
Red=Cloning site Green=Tags(s) MEQVNELKEKGNKALSVGNIDDALQCYSEAIKLDPHNHVLYSNRSAAYAKKGDYQKAYEDGCKTVDLKPD WGKGYSRKAAALEFLNRFEEAKRTYEEGLKHEANNPQLKEGLQNMEARLAERKFMNPFNMPNLYQKLESD PRTRTLLSDPTYRELIEQLRNKPSDLGTKLQDPRIMTTLSVLLGVDLGSMDEEEEIATPPPPPPPKKETK PEPMEEDLPENKKQALKEKELGNDAYKKKDFDTALKHYDKAKELDPTNMTYITNQAAVYFEKGDYNKCRE LCEKAIEVGRENREDYRQIAKAYARIGNSYFKEEKYKDAIHFYNKSLAEHRTPDVLKKCQQAEKILKEQE RLAYINPDLALEEKNKGNECFQKGDYPQAMKHYTEAIKRNPKDAKLYSNRAACYTKLLEFQLALKDCEEC IQLEPTFIKGYTRKAAALEAMKDYTKAMDVYQKALDLDSSCKEAADGYQRCMMAQYNRHDSPEDVKRRAM ADPEVQQIMSDPAMRLILEQMQKDPQALSEHLKNPVIAQKIQKLMDVGLIAIR TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_006819 |
| ORF Size | 1629 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_006819.3 |
| RefSeq Size | 2219 bp |
| RefSeq ORF | 1632 bp |
| Locus ID | 10963 |
| UniProt ID | P31948 |
| Domains | TPR, STI1 |
| Protein Families | Stem cell - Pluripotency |
| Protein Pathways | Prion diseases |
| MW | 62.6 kDa |
| Gene Summary | STIP1 is an adaptor protein that coordinates the functions of HSP70 (see HSPA1A; MIM 140550) and HSP90 (see HSP90AA1; MIM 140571) in protein folding. It is thought to assist in the transfer of proteins from HSP70 to HSP90 by binding both HSP90 and substrate-bound HSP70. STIP1 also stimulates the ATPase activity of HSP70 and inhibits the ATPase activity of HSP90, suggesting that it regulates both the conformations and ATPase cycles of these chaperones (Song and Masison, 2005 [PubMed 16100115]).[supplied by OMIM, Jul 2009] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC201084L1 | Lenti ORF clone of Human stress-induced-phosphoprotein 1 (STIP1), Myc-DDK-tagged |
CNY 6,440.00 |
|
| RC201084L2 | Lenti ORF clone of Human stress-induced-phosphoprotein 1 (STIP1), mGFP tagged |
CNY 5,890.00 |
|
| RC201084L3 | Lenti ORF clone of Human stress-induced-phosphoprotein 1 (STIP1), Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC201084L4 | Lenti ORF clone of Human stress-induced-phosphoprotein 1 (STIP1), mGFP tagged |
CNY 6,440.00 |
|
| RG201084 | STIP1 (tGFP-tagged) - Human stress-induced-phosphoprotein 1 (STIP1) |
CNY 4,370.00 |
|
| SC115872 | STIP1 (untagged)-Human stress-induced-phosphoprotein 1 (STIP1) |
CNY 4,048.00 |
