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Integrin alpha 5 (ITGA5) (NM_002205) Human Tagged ORF Clone
CAT＃: RC201151
ITGA5 (Myc-DDK-tagged)-Human integrin, alpha 5 (fibronectin receptor, alpha polypeptide) (ITGA5)
ORF Plasmid: tGFP
"NM_002205" in other vectors (6)
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CNY 7,304.00
CNY 7,600.00
| Cited in 4 publications. |
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | CD49e; FNRA; VLA-5; VLA5A |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC201151 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGGAGCCGGACGCCAGAGTCCCCTCTCCACGCCGTGCAGCTGCGCTGGGGCCCCCGGCGCCGACCCC CGCTGCTGCCGCTGCTGTTGCTGCTGCTGCCGCCGCCACCCAGGGTCGGGGGCTTCAACTTAGACGCGGA GGCCCCAGCAGTACTCTCGGGGCCCCCGGGCTCCTTCTTCGGATTCTCAGTGGAGTTTTACCGGCCGGGA ACAGACGGGGTCAGTGTGCTGGTGGGAGCACCCAAGGCTAATACCAGCCAGCCAGGAGTGCTGCAGGGTG GTGCTGTCTACCTCTGTCCTTGGGGTGCCAGCCCCACACAGTGCACCCCCATTGAATTTGACAGCAAAGG CTCTCGGCTCCTGGAGTCCTCACTGTCCAGCTCAGAGGGAGAGGAGCCTGTGGAGTACAAGTCCTTGCAG TGGTTCGGGGCAACAGTTCGAGCCCATGGCTCCTCCATCTTGGCATGCGCTCCACTGTACAGCTGGCGCA CAGAGAAGGAGCCACTGAGCGACCCCGTGGGCACCTGCTACCTCTCCACAGATAACTTCACCCGAATTCT GGAGTATGCACCCTGCCGCTCAGATTTCAGCTGGGCAGCAGGACAGGGTTACTGCCAAGGAGGCTTCAGT GCCGAGTTCACCAAGACTGGCCGTGTGGTTTTAGGTGGACCAGGAAGCTATTTCTGGCAAGGCCAGATCC TGTCTGCCACTCAGGAGCAGATTGCAGAATCTTATTACCCCGAGTACCTGATCAACCTGGTTCAGGGGCA GCTGCAGACTCGCCAGGCCAGTTCCATCTATGATGACAGCTACCTAGGATACTCTGTGGCTGTTGGTGAA TTCAGTGGTGATGACACAGAAGACTTTGTTGCTGGTGTGCCCAAAGGGAACCTCACTTACGGCTATGTCA CCATCCTTAATGGCTCAGACATTCGATCCCTCTACAACTTCTCAGGGGAACAGATGGCCTCCTACTTTGG CTATGCAGTGGCCGCCACAGACGTCAATGGGGACGGGCTGGATGACTTGCTGGTGGGGGCACCCCTGCTC ATGGATCGGACCCCTGACGGGCGGCCTCAGGAGGTGGGCAGGGTCTACGTCTACCTGCAGCACCCAGCCG GCATAGAGCCCACGCCCACCCTTACCCTCACTGGCCATGATGAGTTTGGCCGATTTGGCAGCTCCTTGAC CCCCCTGGGGGACCTGGACCAGGATGGCTACAATGATGTGGCCATCGGGGCTCCCTTTGGTGGGGAGACC CAGCAGGGAGTAGTGTTTGTATTTCCTGGGGGCCCAGGAGGGCTGGGCTCTAAGCCTTCCCAGGTTCTGC AGCCCCTGTGGGCAGCCAGCCACACCCCAGACTTCTTTGGCTCTGCCCTTCGAGGAGGCCGAGACCTGGA TGGCAATGGATATCCTGATCTGATTGTGGGGTCCTTTGGTGTGGACAAGGCTGTGGTATACAGGGGCCGC CCCATCGTGTCCGCTAGTGCCTCCCTCACCATCTTCCCCGCCATGTTCAACCCAGAGGAGCGGAGCTGCA GCTTAGAGGGGAACCCTGTGGCCTGCATCAACCTTAGCTTCTGCCTCAATGCTTCTGGAAAACACGTTGC TGACTCCATTGGTTTCACAGTGGAACTTCAGCTGGACTGGCAGAAGCAGAAGGGAGGGGTACGGCGGGCA CTGTTCCTGGCCTCCAGGCAGGCAACCCTGACCCAGACCCTGCTCATCCAGAATGGGGCTCGAGAGGATT GCAGAGAGATGAAGATCTACCTCAGGAACGAGTCAGAATTTCGAGACAAACTCTCGCCGATTCACATCGC TCTCAACTTCTCCTTGGACCCCCAAGCCCCAGTGGACAGCCACGGCCTCAGGCCAGCCCTACATTATCAG AGCAAGAGCCGGATAGAGGACAAGGCTCAGATCTTGCTGGACTGTGGAGAAGACAACATCTGTGTGCCTG ACCTGCAGCTGGAAGTGTTTGGGGAGCAGAACCATGTGTACCTGGGTGACAAGAATGCCCTGAACCTCAC TTTCCATGCCCAGAATGTGGGTGAGGGTGGCGCCTATGAGGCTGAGCTTCGGGTCACCGCCCCTCCAGAG GCTGAGTACTCAGGACTCGTCAGACACCCAGGGAACTTCTCCAGCCTGAGCTGTGACTACTTTGCCGTGA ACCAGAGCCGCCTGCTGGTGTGTGACCTGGGCAACCCCATGAAGGCAGGAGCCAGTCTGTGGGGTGGCCT TCGGTTTACAGTCCCTCATCTCCGGGACACTAAGAAAACCATCCAGTTTGACTTCCAGATCCTCAGCAAG AATCTCAACAACTCGCAAAGCGACGTGGTTTCCTTTCGGCTCTCCGTGGAGGCTCAGGCCCAGGTCACCC TGAACGGTGTCTCCAAGCCTGAGGCAGTGCTATTCCCAGTAAGCGACTGGCATCCCCGAGACCAGCCTCA GAAGGAGGAGGACCTGGGACCTGCTGTCCACCATGTCTATGAGCTCATCAACCAAGGCCCCAGCTCCATT AGCCAGGGTGTGCTGGAACTCAGCTGTCCCCAGGCTCTGGAAGGTCAGCAGCTCCTATATGTGACCAGAG TTACGGGACTCAACTGCACCACCAATCACCCCATTAACCCAAAGGGCCTGGAGTTGGATCCCGAGGGTTC CCTGCACCACCAGCAAAAACGGGAAGCTCCAAGCCGCAGCTCTGCTTCCTCGGGACCTCAGATCCTGAAA TGCCCGGAGGCTGAGTGTTTCAGGCTGCGCTGTGAGCTCGGGCCCCTGCACCAACAAGAGAGCCAAAGTC TGCAGTTGCATTTCCGAGTCTGGGCCAAGACTTTCTTGCAGCGGGAGCACCAGCCATTTAGCCTGCAGTG TGAGGCTGTGTACAAAGCCCTGAAGATGCCCTACCGAATCCTGCCTCGGCAGCTGCCCCAAAAAGAGCGT CAGGTGGCCACAGCTGTGCAATGGACCAAGGCAGAAGGCAGCTATGGCGTCCCACTGTGGATCATCATCC TAGCCATCCTGTTTGGCCTCCTGCTCCTAGGTCTACTCATCTACATCCTCTACAAGCTTGGATTCTTCAA ACGCTCCCTCCCATATGGCACCGCCATGGAAAAAGCTCAGCTCAAGCCTCCAGCCACCTCTGATGCC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC201151 protein sequence
Red=Cloning site Green=Tags(s) MGSRTPESPLHAVQLRWGPRRRPPLLPLLLLLLPPPPRVGGFNLDAEAPAVLSGPPGSFFGFSVEFYRPG TDGVSVLVGAPKANTSQPGVLQGGAVYLCPWGASPTQCTPIEFDSKGSRLLESSLSSSEGEEPVEYKSLQ WFGATVRAHGSSILACAPLYSWRTEKEPLSDPVGTCYLSTDNFTRILEYAPCRSDFSWAAGQGYCQGGFS AEFTKTGRVVLGGPGSYFWQGQILSATQEQIAESYYPEYLINLVQGQLQTRQASSIYDDSYLGYSVAVGE FSGDDTEDFVAGVPKGNLTYGYVTILNGSDIRSLYNFSGEQMASYFGYAVAATDVNGDGLDDLLVGAPLL MDRTPDGRPQEVGRVYVYLQHPAGIEPTPTLTLTGHDEFGRFGSSLTPLGDLDQDGYNDVAIGAPFGGET QQGVVFVFPGGPGGLGSKPSQVLQPLWAASHTPDFFGSALRGGRDLDGNGYPDLIVGSFGVDKAVVYRGR PIVSASASLTIFPAMFNPEERSCSLEGNPVACINLSFCLNASGKHVADSIGFTVELQLDWQKQKGGVRRA LFLASRQATLTQTLLIQNGAREDCREMKIYLRNESEFRDKLSPIHIALNFSLDPQAPVDSHGLRPALHYQ SKSRIEDKAQILLDCGEDNICVPDLQLEVFGEQNHVYLGDKNALNLTFHAQNVGEGGAYEAELRVTAPPE AEYSGLVRHPGNFSSLSCDYFAVNQSRLLVCDLGNPMKAGASLWGGLRFTVPHLRDTKKTIQFDFQILSK NLNNSQSDVVSFRLSVEAQAQVTLNGVSKPEAVLFPVSDWHPRDQPQKEEDLGPAVHHVYELINQGPSSI SQGVLELSCPQALEGQQLLYVTRVTGLNCTTNHPINPKGLELDPEGSLHHQQKREAPSRSSASSGPQILK CPEAECFRLRCELGPLHQQESQSLQLHFRVWAKTFLQREHQPFSLQCEAVYKALKMPYRILPRQLPQKER QVATAVQWTKAEGSYGVPLWIIILAILFGLLLLGLLIYILYKLGFFKRSLPYGTAMEKAQLKPPATSDA TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_002205 |
| ORF Size | 3147 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_002205.5 |
| RefSeq Size | 4267 bp |
| RefSeq ORF | 3150 bp |
| Locus ID | 3678 |
| UniProt ID | P08648 |
| Domains | FG-GAP |
| Protein Families | Druggable Genome, ES Cell Differentiation/IPS, Transmembrane |
| Protein Pathways | Arrhythmogenic right ventricular cardiomyopathy (ARVC), Dilated cardiomyopathy, ECM-receptor interaction, Focal adhesion, Hematopoietic cell lineage, Hypertrophic cardiomyopathy (HCM), Regulation of actin cytoskeleton |
| MW | 114.5 kDa |
| Gene Summary | The product of this gene belongs to the integrin alpha chain family. Integrins are heterodimeric integral membrane proteins composed of an alpha subunit and a beta subunit that function in cell surface adhesion and signaling. The encoded preproprotein is proteolytically processed to generate light and heavy chains that comprise the alpha 5 subunit. This subunit associates with the beta 1 subunit to form a fibronectin receptor. This integrin may promote tumor invasion, and higher expression of this gene may be correlated with shorter survival time in lung cancer patients. Note that the integrin alpha 5 and integrin alpha V subunits are encoded by distinct genes. [provided by RefSeq, Oct 2015] |
Citations (4)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
TGF-β triggers rapid fibrillogenesis via a novel TβRII dependent fibronectin trafficking mechanism
,Varadaraj, A;Jenkins, LM;Singh, P;Chanda, A;Snider, J;Lee, NY;Amsalem-Zafran, AR;Ehrlich, M;Henis, YI;Mythreye, K;,
Mol. Biol. Cell
,PubMed ID 28298487
[Integrin alpha 5]
|
|
Constitutive Association of Tie1 and Tie2 with Endothelial Integrins is Functionally Modulated by Angiopoietin-1 and Fibronectin
,Dalton, AC;Shlamkovitch, T;Papo, N;Barton, WA;,
PLoS ONE
,PubMed ID 27695111
[Integrin alpha 5]
|
|
Effects of integrin a5ß1 on the proliferation and migration of human aortic vascular smooth muscle cells
,Song, Y;Qin, X;Wang, H;Miao, R;Zhang, Y;Miao, C;Wang, Z;,
Mol Med Rep
,PubMed ID 26648324
[Integrin alpha 5]
|
|
CEACAM1 regulates TIM-3-mediated tolerance and exhaustion
,Huang, YH;Zhu, C;Kondo, Y;Anderson, AC;Gandhi, A;,
Nature
[Integrin alpha 5]
|
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC201151L1 | Lenti ORF clone of Human integrin, alpha 5 (fibronectin receptor, alpha polypeptide) (ITGA5), Myc-DDK-tagged |
CNY 9,704.00 |
|
| RC201151L2 | Lenti ORF clone of Human integrin, alpha 5 (fibronectin receptor, alpha polypeptide) (ITGA5), mGFP tagged |
CNY 9,704.00 |
|
| RC201151L3 | Lenti ORF clone of Human integrin, alpha 5 (fibronectin receptor, alpha polypeptide) (ITGA5), Myc-DDK-tagged |
CNY 10,830.00 |
|
| RC201151L4 | Lenti ORF clone of Human integrin, alpha 5 (fibronectin receptor, alpha polypeptide) (ITGA5), mGFP tagged |
CNY 9,704.00 |
|
| RG201151 | ITGA5 (tGFP-tagged) - Human integrin, alpha 5 (fibronectin receptor, alpha polypeptide) (ITGA5) |
CNY 8,904.00 |
|
| SC118748 | ITGA5 (untagged)-Human integrin, alpha 5 (fibronectin receptor, alpha polypeptide) (ITGA5) |
CNY 7,312.00 |
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