United States
Germany
Japan
United Kingdom
China
GET3 (NM_004317) Human Tagged ORF Clone
CAT＃: RC201194
ASNA1 (Myc-DDK-tagged)-Human arsA arsenite transporter, ATP-binding, homolog 1 (bacterial) (ASNA1)
ORF Plasmid: tGFP
"NM_004317" in other vectors (7)
Need custom modification / cloning service?
Get a free quote
CNY 3,656.00
CNY 3,990.00
Product images
CNY 300.00
CNY 6,281.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | ARSA-I; ARSA1; ASNA-I; ASNA1; TRC40 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC201194 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGGCAGGGGTGGCCGGGTGGGGGGTTGAGGCAGAGGAGTTCGAAGATGCTCCTGATGTGGAGCCGC TGGAGCCTACACTTAGCAACATCATCGAGCAGCGCAGCCTGAAGTGGATCTTCGTCGGGGGCAAGGGTGG TGTGGGCAAGACCACCTGCAGCTGCAGCCTGGCAGTCCAGCTCTCCAAGGGGCGTGAGAGTGTTCTGATC ATCTCCACAGACCCAGCACACAACATCTCAGATGCTTTTGACCAGAAGTTCTCAAAGGTGCCTACCAAGG TCAAAGGCTATGACAACCTCTTTGCTATGGAGATTGACCCCAGCCTGGGCGTGGCGGAGCTGCCTGACGA GTTCTTCGAGGAGGACAACATGCTGAGCATGGGCAAGAAGATGATGCAGGAGGCCATGAGCGCATTTCCC GGCATCGATGAGGCCATGAGCTATGCCGAGGTCATGAGGCTGGTGAAGGGCATGAACTTCTCGGTGGTGG TATTTGACACGGCACCCACGGGCCACACCCTGAGGCTGCTCAACTTCCCCACCATCGTGGAGCGGGGCCT GGGCCGGCTTATGCAGATCAAGAACCAGATCAGCCCTTTCATCTCACAGATGTGCAACATGCTGGGCCTG GGGGACATGAACGCAGACCAGCTGGCCTCCAAGCTGGAGGAGACGCTGCCCGTCATCCGCTCAGTCAGCG AACAGTTCAAGGACCCTGAGCAGACAACTTTCATCTGCGTATGCATTGCTGAGTTCCTGTCCCTGTATGA GACAGAGAGGCTGATCCAGGAGCTGGCCAAGTGCAAGATTGACACACACAATATAATTGTCAACCAGCTC GTCTTCCCCGACCCCGAGAAGCCCTGCAAGATGTGTGAGGCCCGTCACAAGATCCAGGCCAAGTATCTGG ACCAGATGGAGGACCTGTATGAAGACTTCCACATCGTGAAGCTGCCGCTGTTACCCCATGAGGTGCGGGG GGCAGACAAGGTCAACACCTTCTCGGCCCTCCTCCTGGAGCCCTACAAGCCCCCCAGTGCCCAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC201194 protein sequence
Red=Cloning site Green=Tags(s) MAAGVAGWGVEAEEFEDAPDVEPLEPTLSNIIEQRSLKWIFVGGKGGVGKTTCSCSLAVQLSKGRESVLI ISTDPAHNISDAFDQKFSKVPTKVKGYDNLFAMEIDPSLGVAELPDEFFEEDNMLSMGKKMMQEAMSAFP GIDEAMSYAEVMRLVKGMNFSVVVFDTAPTGHTLRLLNFPTIVERGLGRLMQIKNQISPFISQMCNMLGL GDMNADQLASKLEETLPVIRSVSEQFKDPEQTTFICVCIAEFLSLYETERLIQELAKCKIDTHNIIVNQL VFPDPEKPCKMCEARHKIQAKYLDQMEDLYEDFHIVKLPLLPHEVRGADKVNTFSALLLEPYKPPSAQ TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_004317 |
| ORF Size | 1044 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_004317.4 |
| RefSeq Size | 1298 bp |
| RefSeq ORF | 1047 bp |
| Locus ID | 439 |
| UniProt ID | O43681 |
| Domains | ArsA_ATPase |
| MW | 38.8 kDa |
| Gene Summary | This gene represents the human homolog of the bacterial arsA gene, encoding the arsenite-stimulated ATPase component of the arsenite transporter responsible for resistance to arsenicals. This protein is also a central component of a transmembrane domain (TMD) recognition complex (TRC) that is involved in the post-translational delivery of tail-anchored (TA) proteins from the cytosol to the endoplasmic reticulum (ER). It recognizes and selectively binds the TMD of TA proteins in the cytosol, and delivers them to the ER for insertion. [provided by RefSeq, Oct 2011] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC201194L1 | Lenti ORF clone of Human arsA arsenite transporter, ATP-binding, homolog 1 (bacterial) (ASNA1), Myc-DDK-tagged |
CNY 6,056.00 |
|
| RC201194L2 | Lenti ORF clone of Human arsA arsenite transporter, ATP-binding, homolog 1 (bacterial) (ASNA1), mGFP tagged |
CNY 5,890.00 |
|
| RC201194L3 | Lenti ORF clone of Human arsA arsenite transporter, ATP-binding, homolog 1 (bacterial) (ASNA1), Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC201194L4 | Lenti ORF clone of Human arsA arsenite transporter, ATP-binding, homolog 1 (bacterial) (ASNA1), mGFP tagged |
CNY 5,890.00 |
|
| RG201194 | ASNA1 (tGFP-tagged) - Human arsA arsenite transporter, ATP-binding, homolog 1 (bacterial) (ASNA1) |
CNY 4,370.00 |
|
| SC317543 | ASNA1 (untagged)-Human arsA arsenite transporter, ATP-binding, homolog 1 (bacterial) (ASNA1) |
CNY 5,610.00 |
|
| SC319116 | ASNA1 (untagged)-Human arsA arsenite transporter, ATP-binding, homolog 1 (bacterial) (ASNA1) |
CNY 3,656.00 |
