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TRIB2 (NM_021643) Human Tagged ORF Clone
CAT＃: RC201210
TRIB2 (Myc-DDK-tagged)-Human tribbles homolog 2 (Drosophila) (TRIB2), transcript variant 1
ORF Plasmid: tGFP
"NM_021643" in other vectors (6)
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CNY 3,656.00
CNY 3,990.00
Product images
CNY 300.00
CNY 1,999.00
CNY 2,700.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | C5FW; GS3955; TRB2 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC201210 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAACATACACAGGTCTACCCCCATCACAATAGCGAGATATGGGAGATCGCGGAACAAAACCCAGGATT TCGAAGAGTTGTCGTCTATAAGGTCCGCGGAGCCCAGCCAGAGTTTCAGCCCGAACCTCGGCTCCCCGAG CCCGCCCGAGACTCCGAACTTGTCGCATTGCGTTTCTTGTATCGGGAAATACTTATTGTTGGAACCTCTG GAGGGAGACCACGTTTTTCGTGCCGTGCATCTGCACAGCGGAGAGGAGCTGGTGTGCAAGGTGTTTGATA TCAGCTGCTACCAGGAATCCCTGGCACCGTGCTTTTGCCTGTCTGCTCATAGTAACATCAACCAAATCAC TGAAATTATCCTGGGTGAGACCAAAGCCTATGTGTTCTTTGAGCGAAGCTATGGGGACATGCATTCCTTC GTCCGCACCTGCAAGAAGCTGAGAGAGGAGGAGGCAGCCAGACTGTTCTACCAGATTGCCTCGGCAGTGG CCCACTGCCATGACGGGGGGCTGGTGCTGCGGGACCTCAAGCTGCGGAAATTCATCTTTAAGGACGAAGA GAGGACTCGGGTCAAGCTGGAAAGCCTGGAAGACGCCTACATTCTGCGGGGAGATGATGATTCCCTCTCC GACAAGCATGGCTGCCCGGCTTACGTAAGCCCAGAGATCTTGAACACCAGTGGCAGCTACTCGGGCAAAG CAGCCGACGTGTGGAGCCTGGGGGTGATGCTGTACACCATGTTGGTGGGGCGGTACCCTTTCCATGACAT TGAACCCAGCTCCCTCTTCAGCAAGATCCGGCGTGGCCAGTTCAACATTCCAGAGACTCTGTCGCCCAAG GCCAAGTGCCTCATCCGAAGCATTCTGCGTCGGGAGCCCTCAGAGCGGCTGACCTCGCAGGAAATTCTGG ACCATCCTTGGTTTTCTACAGATTTTAGCGTCTCGAATTCAGCATATGGTGCTAAGGAAGTGTCTGACCA GCTGGTGCCGGACGTCAACATGGAAGAGAACTTGGACCCTTTCTTTAAC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC201210 protein sequence
Red=Cloning site Green=Tags(s) MNIHRSTPITIARYGRSRNKTQDFEELSSIRSAEPSQSFSPNLGSPSPPETPNLSHCVSCIGKYLLLEPL EGDHVFRAVHLHSGEELVCKVFDISCYQESLAPCFCLSAHSNINQITEIILGETKAYVFFERSYGDMHSF VRTCKKLREEEAARLFYQIASAVAHCHDGGLVLRDLKLRKFIFKDEERTRVKLESLEDAYILRGDDDSLS DKHGCPAYVSPEILNTSGSYSGKAADVWSLGVMLYTMLVGRYPFHDIEPSSLFSKIRRGQFNIPETLSPK AKCLIRSILRREPSERLTSQEILDHPWFSTDFSVSNSAYGAKEVSDQLVPDVNMEENLDPFFN TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_021643 |
| ORF Size | 1029 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_021643.3 |
| RefSeq Size | 4408 bp |
| RefSeq ORF | 1032 bp |
| Locus ID | 28951 |
| UniProt ID | Q92519 |
| Domains | pkinase, TyrKc, S_TKc |
| Protein Families | Druggable Genome, Protein Kinase |
| MW | 38.8 kDa |
| Gene Summary | This gene encodes one of three members of the Tribbles family. The Tribbles members share a Trb domain, which is homologous to protein serine-threonine kinases, but lacks the active site lysine and probably lacks a catalytic function. The Tribbles proteins interact and modulate the activity of signal transduction pathways in a number of physiological and pathological processes. This Tribbles member induces apoptosis of cells mainly of the hematopoietic origin. It has been identified as a protein up-regulated by inflammatory stimuli in myeloid (THP-1) cells, and also as an oncogene that inactivates the transcription factor C/EBPalpha (CCAAT/enhancer-binding protein alpha) and causes acute myelogenous leukemia. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Mar 2009] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC201210L1 | Lenti ORF clone of Human tribbles homolog 2 (Drosophila) (TRIB2), transcript variant 1, Myc-DDK-tagged |
CNY 6,056.00 |
|
| RC201210L2 | Lenti ORF clone of Human tribbles homolog 2 (Drosophila) (TRIB2), transcript variant 1, mGFP tagged |
CNY 5,890.00 |
|
| RC201210L3 | Lenti ORF clone of Human tribbles homolog 2 (Drosophila) (TRIB2), transcript variant 1, Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC201210L4 | Lenti ORF clone of Human tribbles homolog 2 (Drosophila) (TRIB2), transcript variant 1, mGFP tagged |
CNY 6,056.00 |
|
| RG201210 | TRIB2 (tGFP-tagged) - Human tribbles homolog 2 (Drosophila) (TRIB2), transcript variant 1 |
CNY 5,256.00 |
|
| SC111247 | TRIB2 (untagged)-Human tribbles homolog 2 (Drosophila) (TRIB2), transcript variant 1 |
CNY 3,656.00 |
