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DTYMK (NM_012145) Human Tagged ORF Clone
CAT＃: RC201228
DTYMK (Myc-DDK-tagged)-Human deoxythymidylate kinase (thymidylate kinase) (DTYMK), transcript variant 1
ORF Plasmid: tGFP
"NM_012145" in other vectors (6)
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CNY 2,400.00
CNY 3,705.00
Product images
CNY 300.00
CNY 1,999.00
CNY 2,700.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | CDC8; PP3731; TMPK; TYMK |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC201228 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGGCCCGGCGCGGGGCTCTCATAGTGCTGGAGGGCGTGGACCGCGCCGGGAAGAGCACGCAGAGCC GCAAGCTGGTGGAAGCGCTGTGCGCCGCGGGCCACCGCGCCGAACTGCTCCGGTTCCCGGAAAGATCAAC TGAAATCGGCAAACTTCTGAGTTCCTACTTGCAAAAGAAAAGTGACGTGGAGGATCACTCGGTGCACCTG CTTTTTTCTGCAAATCGCTGGGAACAAGTGCCGTTAATTAAGGAAAAGTTGAGCCAGGGCGTGACCCTCG TCGTGGACAGATACGCATTTTCTGGTGTGGCCTTCACCGGTGCCAAGGAGAATTTTTCCCTAGACTGGTG TAAACAGCCAGACGTGGGCCTTCCCAAACCCGACCTGGTCCTGTTCCTCCAGTTACAGCTGGCGGATGCT GCCAAGCGGGGAGCGTTTGGCCATGAGCGCTATGAGAACGGGGCTTTCCAGGAGCGGGCGCTCCGGTGTT TCCACCAGCTCATGAAAGACACGACTTTGAACTGGAAGATGGTGGATGCTTCCAAAAGCATCGAAGCTGT CCATGAGGACATCCGCGTGCTCTCTGAGGACGCCATCCGCACTGCCACAGAGAAGCCGCTGGGGGAGCTA TGGAAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC201228 protein sequence
Red=Cloning site Green=Tags(s) MAARRGALIVLEGVDRAGKSTQSRKLVEALCAAGHRAELLRFPERSTEIGKLLSSYLQKKSDVEDHSVHL LFSANRWEQVPLIKEKLSQGVTLVVDRYAFSGVAFTGAKENFSLDWCKQPDVGLPKPDLVLFLQLQLADA AKRGAFGHERYENGAFQERALRCFHQLMKDTTLNWKMVDASKSIEAVHEDIRVLSEDAIRTATEKPLGEL WK TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_012145 |
| ORF Size | 636 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_012145.4 |
| RefSeq Size | 1227 bp |
| RefSeq ORF | 639 bp |
| Locus ID | 1841 |
| UniProt ID | P23919 |
| Domains | Thymidylate_kin |
| Protein Families | Druggable Genome |
| Protein Pathways | Metabolic pathways, Pyrimidine metabolism |
| MW | 23.8 kDa |
| Gene Summary | Catalyzes the conversion of dTMP to dTDP.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC201228L1 | Lenti ORF clone of Human deoxythymidylate kinase (thymidylate kinase) (DTYMK), transcript variant 1, Myc-DDK-tagged |
CNY 4,800.00 |
|
| RC201228L2 | Lenti ORF clone of Human deoxythymidylate kinase (thymidylate kinase) (DTYMK), transcript variant 1, mGFP tagged |
CNY 5,890.00 |
|
| RC201228L3 | Lenti ORF clone of Human deoxythymidylate kinase (thymidylate kinase) (DTYMK), transcript variant 1, Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC201228L4 | Lenti ORF clone of Human deoxythymidylate kinase (thymidylate kinase) (DTYMK), transcript variant 1, mGFP tagged |
CNY 5,890.00 |
|
| RG201228 | DTYMK (tGFP-tagged) - Human deoxythymidylate kinase (thymidylate kinase) (DTYMK), transcript variant 1 |
CNY 4,000.00 |
|
| SC115536 | DTYMK (untagged)-Human deoxythymidylate kinase (thymidylate kinase) (DTYMK), transcript variant 1 |
CNY 2,400.00 |
