PSMC5 (NM_002805) Human Tagged ORF Clone
CAT#: RC201251
PSMC5 (Myc-DDK-tagged)-Human proteasome (prosome, macropain) 26S subunit, ATPase, 5 (PSMC5), transcript variant 1
ORF Plasmid: tGFP
"NM_002805" in other vectors (6)
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CNY 3,656.00
CNY 3,990.00
Cited in 1 publication. |
CNY 300.00
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | p45; p45/SUG; RPT6; S8; SUG-1; SUG1; TBP10; TRIP1 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RC201251 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGCTTGACGGACCAGAGCAGATGGAGCTGGAGGAGGGGAAGGCAGGCAGCGGACTCCGCCAATATT ATCTGTCCAAGATTGAAGAACTCCAGCTGATTGTGAATGATAAGAGCCAAAACCTCCGGAGGCTGCAGGC ACAGAGGAACGAACTAAATGCTAAAGTTCGCCTATTGCGGGAGGAGCTACAGCTGCTGCAGGAGCAGGGC TCCTATGTGGGGGAAGTAGTCCGGGCCATGGATAAGAAGAAAGTGTTGGTCAAGGTACATCCTGAAGGTA AATTTGTTGTAGACGTGGACAAAAACATTGACATCAATGATGTGACACCCAATTGCCGGGTGGCTCTAAG GAATGACAGCTACACTCTGCACAAGATCCTGCCCAACAAGGTAGACCCATTAGTGTCACTGATGATGGTG GAGAAAGTACCAGATTCAACTTATGAGATGATTGGTGGACTGGACAAACAGATCAAGGAGATCAAAGAAG TGATCGAGCTGCCTGTTAAGCATCCTGAGCTCTTCGAAGCACTGGGCATTGCTCAGCCCAAGGGAGTGCT GCTGTATGGACCTCCAGGCACTGGGAAGACACTGTTGGCCCGGGCTGTGGCTCATCATACGGACTGTACC TTTATTCGTGTCTCTGGCTCTGAACTGGTACAGAAATTCATAGGGGAAGGGGCAAGAATGGTGAGGGAGC TGTTTGTCATGGCACGGGAACATGCTCCATCTATCATCTTCATGGACGAAATCGACTCCATCGGCTCCTC GCGGCTGGAGGGGGGTTCTGGAGGGGACAGTGAAGTGCAGCGCACGATGCTGGAGTTGCTCAACCAGCTC GACGGCTTTGAGGCCACCAAGAACATCAAGGTTATCATGGCTACTAATAGGATTGATATCCTGGACTCGG CACTGCTTCGCCCAGGGCGCATTGACAGAAAAATTGAATTCCCACCCCCCAATGAGGAGGCCCGGCTGGA CATTTTGAAGATTCATTCTCGGAAGATGAACCTGACCCGGGGGATCAACCTGAGAAAAATTGCTGAGCTC ATGCCAGGAGCATCAGGGGCTGAAGTGAAGGGCGTGTGCACAGAAGCTGGCATGTATGCCCTGCGAGAAC GGCGAGTCCATGTCACTCAGGAGGACTTTGAGATGGCAGTAGCCAAGGTCATGCAGAAGGACAGTGAGAA AAACATGTCCATCAAGAAATTATGGAAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC201251 protein sequence
Red=Cloning site Green=Tags(s) MALDGPEQMELEEGKAGSGLRQYYLSKIEELQLIVNDKSQNLRRLQAQRNELNAKVRLLREELQLLQEQG SYVGEVVRAMDKKKVLVKVHPEGKFVVDVDKNIDINDVTPNCRVALRNDSYTLHKILPNKVDPLVSLMMV EKVPDSTYEMIGGLDKQIKEIKEVIELPVKHPELFEALGIAQPKGVLLYGPPGTGKTLLARAVAHHTDCT FIRVSGSELVQKFIGEGARMVRELFVMAREHAPSIIFMDEIDSIGSSRLEGGSGGDSEVQRTMLELLNQL DGFEATKNIKVIMATNRIDILDSALLRPGRIDRKIEFPPPNEEARLDILKIHSRKMNLTRGINLRKIAEL MPGASGAEVKGVCTEAGMYALRERRVHVTQEDFEMAVAKVMQKDSEKNMSIKKLWK TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
![]() Sequencher program is needed, download here. |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_002805 |
ORF Size | 1218 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_002805.6 |
RefSeq Size | 1372 bp |
RefSeq ORF | 1221 bp |
Locus ID | 5705 |
UniProt ID | P62195 |
Domains | AAA, AAA |
Protein Families | Druggable Genome |
Protein Pathways | Proteasome |
MW | 45.6 kDa |
Gene Summary | The 26S proteasome is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. The 20S core is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. The 19S regulator is composed of a base, which contains 6 ATPase subunits and 2 non-ATPase subunits, and a lid, which contains up to 10 non-ATPase subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes one of the ATPase subunits, a member of the triple-A family of ATPases which have a chaperone-like activity. In addition to participation in proteasome functions, this subunit may participate in transcriptional regulation since it has been shown to interact with the thyroid hormone receptor and retinoid X receptor-alpha. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Nov 2010] |
Citations (1)
The use of this cDNA Clones has been cited in the following citations: |
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The autism-linked UBE3A T485A mutant E3 ubiquitin ligase activates the Wnt/β-catenin pathway by inhibiting the proteasome
,Yi, JJ;Paranjape, SR;Walker, MP;Choudhury, R;Wolter, JM;Fragola, G;Emanuele, MJ;Major, MB;Zylka, MJ;,
J. Biol. Chem.
,PubMed ID 28559284
[PSMC5]
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Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC201251L1 | Lenti ORF clone of Human proteasome (prosome, macropain) 26S subunit, ATPase, 5 (PSMC5), transcript variant 1, Myc-DDK-tagged |
CNY 6,056.00 |
|
RC201251L2 | Lenti ORF clone of Human proteasome (prosome, macropain) 26S subunit, ATPase, 5 (PSMC5), transcript variant 1, mGFP tagged |
CNY 5,890.00 |
|
RC201251L3 | Lenti ORF clone of Human proteasome (prosome, macropain) 26S subunit, ATPase, 5 (PSMC5), transcript variant 1, Myc-DDK-tagged |
CNY 6,056.00 |
|
RC201251L4 | Lenti ORF clone of Human proteasome (prosome, macropain) 26S subunit, ATPase, 5 (PSMC5), transcript variant 1, mGFP tagged |
CNY 6,056.00 |
|
RG201251 | PSMC5 (tGFP-tagged) - Human proteasome (prosome, macropain) 26S subunit, ATPase, 5 (PSMC5), transcript variant 1 |
CNY 5,256.00 |
|
SC126754 | PSMC5 (untagged)-Human proteasome (prosome, macropain) 26S subunit, ATPase, 5 (PSMC5), transcript variant 1 |
CNY 3,656.00 |