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FEM1A (NM_018708) Human Tagged ORF Clone
CAT＃: RC201318
FEM1A (Myc-DDK-tagged)-Human fem-1 homolog a (C. elegans) (FEM1A)
ORF Plasmid: tGFP
"NM_018708" in other vectors (5)
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CNY 4,904.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | EPRAP |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC201318 representing NM_018708
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGACCTCCGCACCGCCGTGTACAACGCCGCCCGTGATGGCAAGCTGCAGCTGCTCCAGAAGCTGCTCA GCGGCCGGAGCCGGGAGGAACTGGACGAGCTGACGGGCGAGGTGGCCGGCGGGGGAACGCCGCTACTCAT CGCCGCCCGCTACGGCCACCTGGACGTGGTGGAGTACCTGGTGGACCGGTGCGGCGCGAGCGTGGAGGCC GGTGGCTCGGTGCACTTCGATGGCGAGACCATCGAGGGCGCGCCGCCGCTGTGGGCCGCCTCCGCAGCCG GCCACCTGGACGTGGTGCGGAGCCTGCTGCGCCGCGGGGCCTCGGTGAACCGCACCACGCGCACCAACTC CACGCCTCTCCGCGCCGCCTGCTTCGACGGCCACCTGGAGGTGGTGCGCTACCTGGTCGGCGAGCACCAG GCCGACCTGGAGGTGGCCAACCGGCACGGCCACACGTGCCTCATGATCTCGTGCTACAAGGGCCACCGTG AGATCGCCCGCTACCTGCTGGAGCAGGGCGCCCAGGTGAACCGGCGCAGCGCCAAGGGCAACACGGCCCT GCATGACTGCGCCGAGTCCGGCAGCCTGGAGATCCTGCAGCTGCTGCTGGGGTGCAAGGCCCGCATGGAA CGTGACGGCTACGGCATGACCCCGCTGCTCGCGGCCAGCGTGACGGGCCACACCAACATCGTGGAGTACC TCATCCAGGAGCAGCCCGGCCAGGAGCAGGTCGCAGGGGGAGAGGCTCAGCCTGGGCTGCCCCAAGAAGA CCCCTCCACCAGCCAGGGGTGTGCGCAGCCTCAGGGGGCTCCGTGCTGCAGCTCCTCCCCAGAGGAACCA CTGAACGGGGAATCTTACGAAAGCTGCTGTCCCACCAGCCGGGAAGCTGCCGTGGAAGCCTTGGAATTGC TGGGAGCTACGTATGTGGATAAGAAACGAGATCTGCTTGGGGCCCTTAAACACTGGAGGCGGGCCATGGA GCTGCGTCACCAGGGGGGCGAGTACCTGCCCAAACCGGAGCCCCCACAGCTGGTCCTGGCCTATGACTAT TCCAGGGAGGTCAACACCACCGAGGAGCTGGAGGCGCTGATCACCGACCCGGATGAGATGCGCATGCAGG CCCTGTTGATCCGGGAGCGCATCCTCGGTCCCTCGCACCCGGACACTTCCTATTACATCCGTTACAGGGG TGCCGTGTACGCCGACTCGGGCAATTTCGAGCGCTGCATCCGCTTGTGGAAGTACGCCCTGGACATGCAA CAGAGCAACCTGGAGCCTCTGAGCCCCATGACCGCCAGCAGCTTCCTCTCCTTCGCGGAACTCTTCTCCT ACGTGCTTCAGGACCGGGCCGCCAAAGGCAGCCTGGGCACCCAGATCGGCTTTGCAGACCTCATGGGGGT TCTCACCAAAGGGGTCCGGGAAGTGGAACGGGCCCTGCAGCTGCCCAGGGAGCCCGGAGACTCAGCCCAG TTCACCAAGGCGCTGGCCATCATCCTCCACCTGCTCTACCTGCTGGAGAAAGTGGAGTGCACCCCCAGCC AGGAGCACCTGAAGCACCAGACCGTCTACCGCCTGCTCAAGTGCGCGCCCAGGGGCAAGAACGGCTTCAC CCCTCTGCACATGGCTGTGGACAAGGACACCACAAACGTGGGCCGCTATCCCGTGGGCAGATTCCCCTCC CTGCACGTGGTCAAAGTGCTGCTCGACTGCGGGGCCGACCCGGACAGCAGGGATTTTGACAACAACACCC CGCTACACATAGCAGCCCAGAACAACTGCCCGGCCATCATGAATGCCCTGATCGAAGCAGGGGCCCACAT GGACGCCACCAATGCCTTCAAGAAGACGGCCTACGAGCTGCTGGACGAGAAGCTGCTGGCCAGGGGTACC ATGCAGCCCTTCAACTACGTGACCCTGCAGTGCCTTGCGGCCCGGGCCCTGGATAAGAACAAGATCCCTT ACAAGGGCTTCATCCCGGAAGATCTGGAGGCGTTCATCGAACTGCAC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC201318 representing NM_018708
Red=Cloning site Green=Tags(s) MDLRTAVYNAARDGKLQLLQKLLSGRSREELDELTGEVAGGGTPLLIAARYGHLDVVEYLVDRCGASVEA GGSVHFDGETIEGAPPLWAASAAGHLDVVRSLLRRGASVNRTTRTNSTPLRAACFDGHLEVVRYLVGEHQ ADLEVANRHGHTCLMISCYKGHREIARYLLEQGAQVNRRSAKGNTALHDCAESGSLEILQLLLGCKARME RDGYGMTPLLAASVTGHTNIVEYLIQEQPGQEQVAGGEAQPGLPQEDPSTSQGCAQPQGAPCCSSSPEEP LNGESYESCCPTSREAAVEALELLGATYVDKKRDLLGALKHWRRAMELRHQGGEYLPKPEPPQLVLAYDY SREVNTTEELEALITDPDEMRMQALLIRERILGPSHPDTSYYIRYRGAVYADSGNFERCIRLWKYALDMQ QSNLEPLSPMTASSFLSFAELFSYVLQDRAAKGSLGTQIGFADLMGVLTKGVREVERALQLPREPGDSAQ FTKALAIILHLLYLLEKVECTPSQEHLKHQTVYRLLKCAPRGKNGFTPLHMAVDKDTTNVGRYPVGRFPS LHVVKVLLDCGADPDSRDFDNNTPLHIAAQNNCPAIMNALIEAGAHMDATNAFKKTAYELLDEKLLARGT MQPFNYVTLQCLAARALDKNKIPYKGFIPEDLEAFIELH TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_018708 |
| ORF Size | 2007 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_018708.3 |
| RefSeq Size | 3844 bp |
| RefSeq ORF | 2010 bp |
| Locus ID | 55527 |
| UniProt ID | Q9BSK4 |
| Domains | ANK |
| Protein Families | Druggable Genome |
| MW | 73.5 kDa |
| Gene Summary | Probable component of an E3 ubiquitin-protein ligase complex, in which it may act as a substrate recognition subunit (By similarity). May participate in antiinflammatory signaling via its interaction with PTGER4.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC201318L3 | Lenti-ORF clone of FEM1A (Myc-DDK-tagged)-Human fem-1 homolog a (C. elegans) (FEM1A) |
CNY 5,890.00 |
|
| RC201318L4 | Lenti-ORF clone of FEM1A (mGFP-tagged)-Human fem-1 homolog a (C. elegans) (FEM1A) |
CNY 5,890.00 |
|
| RG201318 | FEM1A (tGFP-tagged) - Human fem-1 homolog a (C. elegans) (FEM1A) |
CNY 4,370.00 |
|
| SC100460 | FEM1A (untagged)-Human fem-1 homolog a (C. elegans) (FEM1A) |
CNY 4,912.00 |
|
| SC320694 | FEM1A (untagged)-Human fem-1 homolog a (C. elegans) (FEM1A) |
CNY 4,912.00 |
