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KIAA1333 (G2E3) (NM_017769) Human Tagged ORF Clone
CAT＃: RC201508
- TrueORF®
G2E3 (Myc-DDK-tagged)-Human G2/M-phase specific E3 ubiquitin protein ligase (G2E3)
ORF Plasmid: tGFP
"NM_017769" in other vectors (4)
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CNY 5,168.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | KIAA1333; PHF7B |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC201508 representing NM_017769
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAATGAAAGTAAACCTGGTGACTCACAGAACCTTGCTTGTGTTTTCTGTCGAAAACATGATGACTGTC CTAATAAATACGGAGAAAAGAAAACTAAGGAGAAATGGAATCTCACTGTACATTACTACTGTTTGTTGAT GTCAAGTGGAATTTGGCAGAGAGGCAAAGAAGAAGAAGGAGTTTATGGTTTTCTAATAGAAGATATCAGG AAGGAAGTGAATAGAGCTTCTAAACTGAAATGCTGTGTTTGCAAGAAAAATGGTGCTTCAATTGGATGTG TTGCACCCCGATGTAAACGAAGTTATCATTTCCCATGTGGACTTCAGAGAGAATGTATTTTCCAGTTTAC TGGCAATTTTGCGTCATTTTGTTGGGACCATCGACCTGTTCAAATAATTACATCTAATAATTATAGAGAG TCCTTACCATGCACCATTTGCTTGGAATTTATTGAGCCTATTCCAAGTTATAACATATTACGAAGTCCTT GTTGTAAGAACGCTTGGTTTCATAGAGACTGTTTACAGGTTCAAGCAATAAATGCGGGAGTGTTTTTCTT TAGGTGTACAATATGCAATAATAGTGACATCTTTCAGAAAGAGATGTTGAGAATGGGAATTCATATTCCT GAAAAAGATGCTTCCTGGGAATTAGAGGAAAACGCTTATCAAGAGCTTCTGCAGCACTATGAGCGTTGTG ATGTTCGAAGATGTCGTTGCAAAGAAGGGCGAGACTATAATGCACCTGATAGCAAATGGGAAATAAAGCG CTGTCAGTGTTGTGGTTCCAGTGGCACACATTTAGCCTGCTCCTCATTACGGTCATGGGAGCAAAATTGG GAGTGTTTGGAATGTAGGGGTATTATCTACAATTCAGGAGAGTTCCAAAAAGCCAAAAAACATGTATTAC CCAATTCTAATAATGTGGGGATTACAGATTGTTTGTTGGAAGAGTCATCACCTAAATTACCCAGACAGTC ACCTGGATCCCAGAGTAAAGATCTACTGAGGCAAGGCAGCAAATTTAGAAGAAATGTATCAACACTATTA ATAGAGTTAGGATTCCAAATTAAAAAAAAAACTAAAAGATTGTATATCAACAAAGCCAATATCTGGAATA GTGCCTTAGATGCATTCAGAAATCGAAACTTTAATCCTTCATATGCAATTGAAGTAGCATATGTTATTGA AAATGATAATTTTGGAAGTGAGCATCCTGGATCAAAGCAAGAATTTCTGAGTCTCTTAATGCAACATCTT GAGAACTCATCATTGTTTGAAGGGTCCTTGTCAAAGAACTTGTCTCTAAATTCTCAAGCTCTGAAAGAGA ATCTTTACTATGAAGCTGGCAAAATGCTTGCCATTTCTTTAGTTCACGGTGGTCCTTCACCTGGTTTCTT TTCTAAAACCTTGTTTAACTGCCTTGTTTATGGACCAGAAAATACCCAGCCAATTTTAGATGATGTTTCA GACTTTGATGTGGCACAGATTATAATCAGGATAAATACTGCAACAACTGTAGCTGACTTAAAGTCAATAA TAAATGAATGCTATAACTACCTTGAGTTAATTGGATGTCTCAGACTTATAACGACATTAAGTGATAAATA TATGTTAGTAAAAGACATACTTGGCTACCATGTAATTCAGAGAGTCCACACACCCTTTGAAAGTTTTAAG CAGGGTCTGAAAACCCTTGGTGTTTTGGAGAAAATTCAGGCTTATCCAGAAGCATTTTGTAGCATCCTGT GTCATAAACCTGAGAGTCTTTCTGCAAAAATCCTTAGTGAGCTTTTTACAGTACACACATTACCTGATGT GAAAGCTTTGGGGTTTTGGAACAGTTACTTACAGGCTGTTGAAGATGGTAAATCTACAACAACAATGGAA GACATTCTTATTTTTGCAACTGGTTGCAGTTCCATTCCTCCAGCTGGATTTAAACCCACTCCTTCAATTG AGTGTCTGCATGTGGATTTTCCTGTTGGAAACAAGTGTAATAACTGTTTAGCAATTCCCATCACCAATAC ATATAAAGAGTTTCAAGAAAATATGGACTTCACCATAAGAAACACTCTAAGACTAGAAAAGGAAGAAAGT TCTCATTACATTGGACAT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC201508 representing NM_017769
Red=Cloning site Green=Tags(s) MNESKPGDSQNLACVFCRKHDDCPNKYGEKKTKEKWNLTVHYYCLLMSSGIWQRGKEEEGVYGFLIEDIR KEVNRASKLKCCVCKKNGASIGCVAPRCKRSYHFPCGLQRECIFQFTGNFASFCWDHRPVQIITSNNYRE SLPCTICLEFIEPIPSYNILRSPCCKNAWFHRDCLQVQAINAGVFFFRCTICNNSDIFQKEMLRMGIHIP EKDASWELEENAYQELLQHYERCDVRRCRCKEGRDYNAPDSKWEIKRCQCCGSSGTHLACSSLRSWEQNW ECLECRGIIYNSGEFQKAKKHVLPNSNNVGITDCLLEESSPKLPRQSPGSQSKDLLRQGSKFRRNVSTLL IELGFQIKKKTKRLYINKANIWNSALDAFRNRNFNPSYAIEVAYVIENDNFGSEHPGSKQEFLSLLMQHL ENSSLFEGSLSKNLSLNSQALKENLYYEAGKMLAISLVHGGPSPGFFSKTLFNCLVYGPENTQPILDDVS DFDVAQIIIRINTATTVADLKSIINECYNYLELIGCLRLITTLSDKYMLVKDILGYHVIQRVHTPFESFK QGLKTLGVLEKIQAYPEAFCSILCHKPESLSAKILSELFTVHTLPDVKALGFWNSYLQAVEDGKSTTTME DILIFATGCSSIPPAGFKPTPSIECLHVDFPVGNKCNNCLAIPITNTYKEFQENMDFTIRNTLRLEKEES SHYIGH TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_017769 |
| ORF Size | 2118 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_017769.5 |
| RefSeq Size | 5581 bp |
| RefSeq ORF | 2121 bp |
| Locus ID | 55632 |
| UniProt ID | Q7L622 |
| Domains | HECT |
| Protein Families | Druggable Genome, Transcription Factors |
| MW | 80.5 kDa |
| Gene Summary | E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. Essential in early embryonic development to prevent apoptotic death.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC201508L3 | Lenti-ORF clone of G2E3 (Myc-DDK-tagged)-Human G2/M-phase specific E3 ubiquitin protein ligase (G2E3) |
CNY 5,890.00 |
|
| RC201508L4 | Lenti-ORF clone of G2E3 (mGFP-tagged)-Human G2/M-phase specific E3 ubiquitin protein ligase (G2E3) |
CNY 5,890.00 |
|
| RG201508 | G2E3 (tGFP-tagged) - Human G2/M-phase specific E3 ubiquitin protein ligase (G2E3) |
CNY 6,768.00 |
|
| SC113933 | G2E3 (untagged)-Human G2/M-phase specific E3 ubiquitin protein ligase (G2E3) |
CNY 5,176.00 |
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