BRCC45 (BRE) (NM_199194) Human Tagged ORF Clone

CAT#: RC201509

BRE (Myc-DDK-tagged)-Human brain and reproductive organ-expressed (TNFRSF1A modulator) (BRE), transcript variant 2

ORF Plasmid: DDK tGFP



  "NM_199194" in other vectors (4)

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CNY 3,656.00

CNY 3,990.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (5)
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

CNY 5,420.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00


DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 30 ul

CNY 300.00


Rabbit Polyclonal Anti-BRE Antibody
    • 100 ul

CNY 1,999.00
CNY 3,280.00

Specifications

Product Data
Type Human Tagged ORF Clone
Tag Myc-DDK
Synonyms BRCC4; BRCC45; BRE
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RC201509 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTCCCCAGAAGTGGCCTTGAACCGAATATCTCCAATGCTCTCCCCTTTCATATCTAGCGTGGTCCGGA
ATGGAAAAGTGGGACTGGATGCTACAAACTGTTTGAGGATAACTGACTTAAAATCTGGCTGCACATCATT
GACTCCTGGGCCCAACTGTGACCGATTTAAACTGCACATACCATATGCTGGAGAGACATTAAAGTGGGAT
ATCATTTTCAATGCCCAATACCCAGAACTGCCTCCCGATTTTATCTTTGGAGAAGATGCTGAATTCCTGC
CAGACCCCTCAGCTTTGCAGAATCTTGCCTCCTGGAATCCTTCAAATCCTGAATGTCTCTTACTTGTGGT
GAAGGAACTTGTGCAACAATATCACCAATTCCAATGTAGCCGCCTCCGGGAGAGCTCCCGCCTCATGTTT
GAATACCAGACATTACTGGAGGAGCCACAGTATGGAGAGAACATGGAAATTTATGCTGGGAAAAAAAACA
ACTGGACTGGTGAATTTTCAGCTCGTTTCCTTTTGAAGCTGCCCGTAGATTTCAGCAATATCCCCACATA
CCTTCTCAAGGATGTAAATGAAGACCCTGGAGAAGATGTGGCCCTCCTCTCTGTTAGTTTTGAGGACACT
GAAGCCACCCAGGTGTACCCCAAGCTGTACTTGTCACCTCGAATTGAGCATGCACTTGGAGGCTCCTCAG
CTCTTCATATCCCAGCTTTTCCAGGAGGAGGATGTCTCATTGATTACGTTCCTCAAGTATGCCACCTGCT
CACCAACAAGGTGCAGTACGTGATTCAAGGGTATCACAAAAGAAGAGAGTATATTGCTGCTTTTCTCAGT
CACTTTGGCACAGGTGTCGTGGAATATGATGCAGAAGGCTTTACAAAACTCACTCTGCTGCTGATGTGGA
AAGATTTTTGTTTTCTTGTACACATTGACCTGCCTCTGTTTTTCCCTCGAGACCAGCCAACTCTCACATT
TCAGTCCGTTTATCACTTTACCAACAGTGGACAGCTTTACTCCCAGGCCCAAAAAAATTATCCGTACAGC
CCCAGATGGGATGGAAATGAAATGGCCAAAAGAGCAAAGGCTTATTTCAAAACCTTTGTCCCTCAGTTCC
AGGAGGCAGCATTTGCCAATGGAAAGCTC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RC201509 protein sequence
Red=Cloning site Green=Tags(s)

MSPEVALNRISPMLSPFISSVVRNGKVGLDATNCLRITDLKSGCTSLTPGPNCDRFKLHIPYAGETLKWD
IIFNAQYPELPPDFIFGEDAEFLPDPSALQNLASWNPSNPECLLLVVKELVQQYHQFQCSRLRESSRLMF
EYQTLLEEPQYGENMEIYAGKKNNWTGEFSARFLLKLPVDFSNIPTYLLKDVNEDPGEDVALLSVSFEDT
EATQVYPKLYLSPRIEHALGGSSALHIPAFPGGGCLIDYVPQVCHLLTNKVQYVIQGYHKRREYIAAFLS
HFGTGVVEYDAEGFTKLTLLLMWKDFCFLVHIDLPLFFPRDQPTLTFQSVYHFTNSGQLYSQAQKNYPYS
PRWDGNEMAKRAKAYFKTFVPQFQEAAFANGKL

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_199194
ORF Size 1149 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_199194.2
RefSeq Size 1870 bp
RefSeq ORF 1152 bp
Locus ID 9577
UniProt ID Q9NXR7
Protein Families Druggable Genome
MW 43.6 kDa
Gene Summary This gene encodes an anti-apoptotic, death receptor-associated protein that interacts with tumor necrosis factor-receptor-1. The encoded protein acts as an adapter in several protein complexes, including the BRCA1-A complex and the BRISC complex. The BRCA1-A complex possesses ubiquitinase activity and targets sites of double strand DNA breaks, while the BRISC complex exhibits deubiquitinase activity and is involved in mitotic spindle assembly. This gene is upregulated in several types of cancer. [provided by RefSeq, Jun 2016]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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