SOX15 (NM_006942) Human Tagged ORF Clone

CAT#: RC201586

SOX15 (Myc-DDK-tagged)-Human SRY (sex determining region Y)-box 15 (SOX15)

ORF Plasmid: DDK tGFP



  "NM_006942" in other vectors (4)

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CNY 2,400.00

CNY 3,705.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (5)
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

CNY 5,420.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00


DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 30 ul

CNY 300.00


Rabbit polyclonal SOX15 Antibody (Center)
    • 400 ul

CNY 6,314.00

Specifications

Product Data
Type Human Tagged ORF Clone
Tag Myc-DDK
Synonyms SOX20; SOX26; SOX27
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RC201586 representing NM_006942
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCGCTACCAGGCTCCTCACAGGACCAGGCCTGGAGCCTGGAGCCTCCGGCTGCCACGGCTGCTGCCT
CCTCATCTTCGGGACCCCAGGAGCGGGAGGGCGCTGGGAGCCCCGCGGCCCCCGGGACGCTGCCCCTGGA
GAAGGTGAAGCGGCCGATGAACGCGTTCATGGTGTGGAGCTCCGCTCAGCGCCGCCAGATGGCGCAGCAG
AACCCCAAGATGCACAACTCCGAGATCTCCAAGCGCCTGGGCGCGCAGTGGAAGCTGCTGGACGAGGACG
AGAAGCGGCCCTTCGTGGAGGAGGCCAAGCGGCTCCGCGCCCGACACCTGCGCGACTACCCCGACTACAA
GTACCGGCCTCGGCGCAAGGCCAAGAGCTCGGGCGCCGGACCTTCCCGCTGCGGACAGGGAAGAGGCAAC
CTGGCCAGCGGCGGCCCGCTCTGGGGGCCGGGGTACGCGACCACCCAACCGAGCAGAGGCTTTGGGTACA
GACCCCCCAGCTACTCGACAGCCTACCTGCCTGGCAGCTATGGCTCTTCCCACTGCAAACTGGAAGCCCC
CTCACCGTGCTCCCTCCCTCAGAGTGACCCTAGGCTCCAGGGGGAACTGCTGCCCACCTATACCCACTAC
CTGCCCCCTGGCTCTCCCACTCCATACAACCCTCCCCTTGCTGGTGCCCCCATGCCCCTAACCCACCTC


AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC
TGGATTACAAGGATGACGACGATAAG
GTTTAA
>RC201586 representing NM_006942
Red=Cloning site Green=Tags(s)

MALPGSSQDQAWSLEPPAATAAASSSSGPQEREGAGSPAAPGTLPLEKVKRPMNAFMVWSSAQRRQMAQQ
NPKMHNSEISKRLGAQWKLLDEDEKRPFVEEAKRLRARHLRDYPDYKYRPRRKAKSSGAGPSRCGQGRGN
LASGGPLWGPGYATTQPSRGFGYRPPSYSTAYLPGSYGSSHCKLEAPSPCSLPQSDPRLQGELLPTYTHY
LPPGSPTPYNPPLAGAPMPLTHL

SGPTRTRRLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-RsrII      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_006942
ORF Size 699 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_006942.2
RefSeq Size 1394 bp
RefSeq ORF 702 bp
Locus ID 6665
UniProt ID O60248
Protein Families Transcription Factors
MW 25.1 kDa
Gene Summary This gene encodes a member of the SOX (SRY-related HMG-box) family of transcription factors involved in the regulation of embryonic development and in the determination of the cell fate. The encoded protein may act as a transcriptional regulator after forming a protein complex with other proteins. [provided by RefSeq, Jul 2008]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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