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PP5 (PPP5C) (NM_006247) Human Tagged ORF Clone
CAT＃: RC201650
PPP5C (Myc-DDK-tagged)-Human protein phosphatase 5, catalytic subunit (PPP5C), transcript variant 1
ORF Plasmid: tGFP
"NM_006247" in other vectors (6)
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CNY 3,656.00
CNY 3,990.00
Product images
CNY 300.00
CNY 1,999.00
CNY 2,700.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | PP5; PPP5; PPT |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC201650 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGATGGCGGAGGGCGAGAGGACTGAGTGTGCTGAGCCCCCCCGGGACGAACCCCCGGCTGATGGAG CTCTGAAGCGGGCAGAGGAGCTCAAGACTCAGGCCAATGACTACTTCAAAGCCAAGGACTACGAGAACGC CATCAAGTTCTACAGCCAGGCCATCGAGCTGAACCCCAGCAATGCCATCTACTATGGCAACCGCAGCCTG GCCTACCTGCGCACTGAGTGCTATGGCTACGCGCTGGGAGACGCCACGCGGGCCATTGAGCTGGACAAGA AGTACATCAAGGGTTATTACCGCCGGGCTGCCAGCAACATGGCACTGGGCAAGTTCCGGGCCGCGCTGCG AGACTACGAGACGGTGGTCAAGGTGAAGCCCCATGACAAGGATGCCAAAATGAAATACCAGGAGTGCAAC AAGATCGTGAAGCAGAAGGCCTTTGAGCGGGCCATCGCGGGCGACGAGCACAAGCGCTCCGTGGTGGACT CGCTGGACATCGAGAGCATGACCATTGAGGATGAGTACAGCGGACCCAAGCTTGAAGACGGCAAAGTGAC AATCAGTTTCATGAAGGAGCTCATGCAGTGGTACAAGGACCAGAAGAAACTGCACCGGAAATGTGCCTAC CAGATTCTGGTACAGGTCAAAGAGGTCCTCTCCAAGCTGAGCACGCTCGTGGAAACCACACTCAAAGAGA CAGAGAAGATTACAGTATGTGGGGACACCCATGGCCAGTTCTATGACCTCCTCAACATATTCGAGCTCAA CGGTTTACCCTCGGAGACCAACCCCTATATATTTAATGGTGACTTTGTGGACCGAGGCTCCTTCTCTGTA GAAGTGATCCTCACCCTTTTCGGCTTCAAGCTCCTGTACCCAGATCACTTTCACCTCCTTCGAGGCAACC ACGAGACAGACAACATGAACCAGATCTACGGTTTCGAGGGTGAGGTGAAGGCCAAGTACACAGCCCAGAT GTACGAGCTCTTTAGCGAGGTGTTCGAGTGGCTCCCGTTGGCCCAGTGCATCAACGGCAAAGTGCTGATC ATGCACGGAGGCCTGTTCAGTGAAGACGGTGTCACCCTGGATGACATCCGGAAAATTGAGCGGAATCGAC AACCCCCAGATTCAGGGCCCATGTGTGACCTGCTCTGGTCAGATCCACAGCCACAGAACGGGCGCTCGAT CAGCAAGCGGGGCGTGAGCTGTCAGTTTGGGCCTGACGTCACCAAGGCCTTCTTGGAAGAGAACAACCTG GACTATATCATCCGCAGCCACGAAGTCAAGGCCGAGGGCTACGAGGTGGCTCACGGAGGCCGCTGTGTCA CCGTCTTCTCTGCCCCCAACTACTGCGACCAGATGGGGAACAAAGCCTCCTACATCCACCTCCAGGGCTC TGACCTACGGCCTCAGTTCCACCAGTTCACAGCAGTGCCTCATCCCAACGTCAAGCCCATGGCCTATGCC AACACGCTGCTGCAGCTAGGAATGATG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC201650 protein sequence
Red=Cloning site Green=Tags(s) MAMAEGERTECAEPPRDEPPADGALKRAEELKTQANDYFKAKDYENAIKFYSQAIELNPSNAIYYGNRSL AYLRTECYGYALGDATRAIELDKKYIKGYYRRAASNMALGKFRAALRDYETVVKVKPHDKDAKMKYQECN KIVKQKAFERAIAGDEHKRSVVDSLDIESMTIEDEYSGPKLEDGKVTISFMKELMQWYKDQKKLHRKCAY QILVQVKEVLSKLSTLVETTLKETEKITVCGDTHGQFYDLLNIFELNGLPSETNPYIFNGDFVDRGSFSV EVILTLFGFKLLYPDHFHLLRGNHETDNMNQIYGFEGEVKAKYTAQMYELFSEVFEWLPLAQCINGKVLI MHGGLFSEDGVTLDDIRKIERNRQPPDSGPMCDLLWSDPQPQNGRSISKRGVSCQFGPDVTKAFLEENNL DYIIRSHEVKAEGYEVAHGGRCVTVFSAPNYCDQMGNKASYIHLQGSDLRPQFHQFTAVPHPNVKPMAYA NTLLQLGMM TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_006247 |
| ORF Size | 1497 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_006247.4 |
| RefSeq Size | 2232 bp |
| RefSeq ORF | 1500 bp |
| Locus ID | 5536 |
| UniProt ID | P53041 |
| Domains | TPR, Metallophos |
| Protein Families | Druggable Genome, Transcription Factors |
| Protein Pathways | MAPK signaling pathway |
| MW | 56.9 kDa |
| Gene Summary | This gene encodes a serine/threonine phosphatase which is a member of the protein phosphatase catalytic subunit family. Proteins in this family participate in pathways regulated by reversible phosphorylation at serine and threonine residues; many of these pathways are involved in the regulation of cell growth and differentiation. The product of this gene has been shown to participate in signaling pathways in response to hormones or cellular stress, and elevated levels of this protein may be associated with breast cancer development. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Feb 2011] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC201650L1 | Lenti ORF clone of Human protein phosphatase 5, catalytic subunit (PPP5C), transcript variant 1, Myc-DDK-tagged |
CNY 6,056.00 |
|
| RC201650L2 | Lenti ORF clone of Human protein phosphatase 5, catalytic subunit (PPP5C), transcript variant 1, mGFP tagged |
CNY 5,890.00 |
|
| RC201650L3 | Lenti ORF clone of Human protein phosphatase 5, catalytic subunit (PPP5C), transcript variant 1, Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC201650L4 | Lenti ORF clone of Human protein phosphatase 5, catalytic subunit (PPP5C), transcript variant 1, mGFP tagged |
CNY 5,890.00 |
|
| RG201650 | PPP5C (tGFP-tagged) - Human protein phosphatase 5, catalytic subunit (PPP5C), transcript variant 1 |
CNY 5,256.00 |
|
| SC323793 | PPP5C (untagged)-Human protein phosphatase 5, catalytic subunit (PPP5C), transcript variant 1 |
CNY 3,656.00 |
