Thioredoxin 2 (TXN2) (NM_012473) Human Tagged ORF Clone

CAT#: RC201666

TXN2 (Myc-DDK-tagged)-Human thioredoxin 2 (TXN2), nuclear gene encoding mitochondrial protein

ORF Plasmid: DDK tGFP



  "NM_012473" in other vectors (6)

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CNY 1,200.00

CNY 3,705.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (5)
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

CNY 5,420.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00


DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 30 ul

CNY 300.00


Thioredoxin 2 Rabbit monoclonal Antibody
    • 100 ul

CNY 1,999.00
CNY 3,280.00

Specifications

Product Data
Type Human Tagged ORF Clone
Tag Myc-DDK
Synonyms COXPD29; MT-TRX; MTRX; TRX2; TXN
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RC201666 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCTCAGCGACTTCTTCTGAGGAGGTTCCTGGCCTCTGTCATCTCCAGGAAGCCCTCTCAGGGTCAGT
GGCCACCCCTCACTTCCAGAGCCCTGCAGACCCCACAATGCAGTCCTGGTGGCCTGACTGTAACACCCAA
CCCAGCCCGGACAATATACACCACGAGGATCTCCTTGACAACCTTTAATATCCAGGATGGACCTGACTTT
CAAGACCGAGTGGTCAACAGTGAGACACCAGTGGTTGTGGATTTCCACGCACAGTGGTGTGGACCCTGCA
AGATCCTGGGGCCGAGGTTAGAGAAGATGGTGGCCAAGCAGCACGGGAAGGTGGTGATGGCCAAGGTGGA
TATTGATGACCACACAGACCTCGCCATTGAGTATGAGGTGTCAGCGGTGCCCACTGTGCTGGCCATGAAG
AATGGGGACGTGGTGGACAAGTTTGTGGGCATCAAGGATGAGGATCAGTTGGAGGCCTTCCTGAAGAAGC
TGATTGGC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RC201666 protein sequence
Red=Cloning site Green=Tags(s)

MAQRLLLRRFLASVISRKPSQGQWPPLTSRALQTPQCSPGGLTVTPNPARTIYTTRISLTTFNIQDGPDF
QDRVVNSETPVVVDFHAQWCGPCKILGPRLEKMVAKQHGKVVMAKVDIDDHTDLAIEYEVSAVPTVLAMK
NGDVVDKFVGIKDEDQLEAFLKKLIG

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_012473
ORF Size 498 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_012473.4
RefSeq Size 1342 bp
RefSeq ORF 501 bp
Locus ID 25828
UniProt ID Q99757
Domains thiored
Protein Families Druggable Genome
MW 18.4 kDa
Gene Summary This nuclear gene encodes a mitochondrial member of the thioredoxin family, a group of small multifunctional redox-active proteins. The encoded protein may play important roles in the regulation of the mitochondrial membrane potential and in protection against oxidant-induced apoptosis. [provided by RefSeq, Jul 2008]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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