United States
Germany
Japan
United Kingdom
China
SACM1L (NM_014016) Human Tagged ORF Clone
CAT＃: RC201684
SACM1L (Myc-DDK-tagged)-Human SAC1 suppressor of actin mutations 1-like (yeast) (SACM1L)
ORF Plasmid: tGFP
"NM_014016" in other vectors (6)
Need custom modification / cloning service?
Get a free quote
CNY 6,552.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | SAC1 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC201684 representing NM_014016
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGACGGCGGCCTACGAGCAGCTGAAGCTGCATATCACACCTGAAAAATTTTATGTGGAAGCTTGTG ATGATGGAGCAGATGACGTACTTACCATTGACCGTGTGTCCACAGAGGTTACCCTTGCAGTCAAGAAAGA TGTTCCTCCTTCAGCTGTCACAAGACCAATATTTGGTATACTGGGCACAATCCATCTGGTGGCAGGTAAT TATCTTATAGTCATTACCAAAAAGATAAAAGTAGGTGAATTTTTCAGTCATGTAGTCTGGAAAGCAACAG ATTTTGATGTCCTTTCTTATAAGAAGACAATGTTGCACTTAACTGATATTCAGTTACAAGATAATAAAAC CTTCCTAGCGATGCTAAACCATGTCTTGAATGTGGATGGATTTTACTTTTCAACAACATATGATTTGACC CATACTTTGCAGCGGCTATCCAACACTAGTCCTGAATTCCAAGAAATGAGTCTCTTGGAAAGGGCAGATC AGCGGTTTGTATGGAATGGTCATCTTCTAAGAGAACTTTCTGCACAGCCAGAGGTTCATCGGTTTGCCCT TCCAGTGTTACATGGCTTTATTACCATGCATTCATGTTCTATTAATGGAAAATACTTTGATTGGATTCTC ATCTCGAGGAGGAGCTGTTTCAGAGCTGGTGTGCGCTATTATGTAAGAGGAATTGATTCGGAAGGCCATG CAGCTAACTTTGTAGAAACAGAACAAATTGTGCACTACAATGGGAGCAAAGCTTCGTTTGTACAGACTCG AGGATCAATACCTGTTTTCTGGTCCCAAAGACCAAACCTCAAGTACAAACCACTGCCACAGATCAGCAAA GTAGCAAATCACATGGACGGTTTCCAAAGGCATTTTGATTCCCAAGTAATTATTTATGGAAAACAAGTTA TAATCAATCTGATTAACCAGAAGGGCTCGGAGAAGCCACTTGAGCAGACATTTGCAACAATGGTGTCTTC CTTGGGAAGTGGAATGATGAGATACATTGCCTTTGACTTCCATAAGGAATGTAAAAATATGAGATGGGAT CGACTAAGTATTTTATTGGATCAGGTAGCAGAAATGCAAGATGAATTAAGTTATTTTCTAGTGGACTCTG CTGGCCAGGTGGTGGCAAACCAGGAAGGCGTGTTCCGAAGCAATTGCATGGATTGTCTAGATAGAACCAA TGTGATCCAGAGTTTGTTAGCTCGTCGTTCACTTCAGGCCCAACTTCAGAGACTAGGAGTTTTGCATGTG GGACAAAAGCTTGAAGAACAAGATGAATTTGAGAAGATTTTCAAAAATGCCTGGGCTGACAACGCAAATG CTTGTGCCAAGCAATATGCGGGAACTGGTGCCTTGAAGACTGACTTTACCAGAACTGGAAAGAGAACTCA TTTGGGACTTATAATGGATGGCTGGAACTCAATGATACGATATTATAAGAACAACTTTTCCGATGGATTT AGACAAGATTCCATAGACTTATTTCTTGGAAACTATTCAGTGGATGAATTAGAATCTCATAGTCCTTTAA GTGTTCCAAGGGACTGGAAATTCCTGGCTTTGCCTATTATCATGGTTGTTGCCTTTTCAATGTGCATTAT CTGTTTGCTTATGGCTGGTGACACTTGGACAGAAACACTGGCCTATGTGCTCTTCTGGGGAGTTGCAAGC ATTGGAACATTTTTTATCATTCTTTACAATGGCAAAGATTTTGTCGATGCTCCCAGACTGGTCCAGAAAG AAAAGATAGAC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC201684 representing NM_014016
Red=Cloning site Green=Tags(s) MATAAYEQLKLHITPEKFYVEACDDGADDVLTIDRVSTEVTLAVKKDVPPSAVTRPIFGILGTIHLVAGN YLIVITKKIKVGEFFSHVVWKATDFDVLSYKKTMLHLTDIQLQDNKTFLAMLNHVLNVDGFYFSTTYDLT HTLQRLSNTSPEFQEMSLLERADQRFVWNGHLLRELSAQPEVHRFALPVLHGFITMHSCSINGKYFDWIL ISRRSCFRAGVRYYVRGIDSEGHAANFVETEQIVHYNGSKASFVQTRGSIPVFWSQRPNLKYKPLPQISK VANHMDGFQRHFDSQVIIYGKQVIINLINQKGSEKPLEQTFATMVSSLGSGMMRYIAFDFHKECKNMRWD RLSILLDQVAEMQDELSYFLVDSAGQVVANQEGVFRSNCMDCLDRTNVIQSLLARRSLQAQLQRLGVLHV GQKLEEQDEFEKIFKNAWADNANACAKQYAGTGALKTDFTRTGKRTHLGLIMDGWNSMIRYYKNNFSDGF RQDSIDLFLGNYSVDELESHSPLSVPRDWKFLALPIIMVVAFSMCIICLLMAGDTWTETLAYVLFWGVAS IGTFFIILYNGKDFVDAPRLVQKEKID TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_014016 |
| ORF Size | 1761 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_014016.5 |
| RefSeq Size | 3550 bp |
| RefSeq ORF | 1764 bp |
| Locus ID | 22908 |
| UniProt ID | Q9NTJ5 |
| Domains | Syja_N |
| Protein Families | Druggable Genome, Transmembrane |
| MW | 66.8 kDa |
| Gene Summary | This gene encodes an integral membrane protein, which is localized to the endoplasmic reticulum, and functions as a phosphoinositide phosphatase that hydrolyzes phosphatidylinositol 3-phosphate, phosphatidylinositol 4-phosphate, and phosphatidylinositol 3,5-bisphosphate. Deletion of this gene in mouse results in preimplantation lethality. Other studies suggest that this gene is also involved in the organization of golgi membranes and mitotic spindles. Alternatively spliced transcript variants have been found for this gene. A C-terminally extended isoform is also predicted to be produced by the use of an alternative in-frame, downstream translation termination codon via a stop codon readthrough mechanism.[provided by RefSeq, Dec 2017] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC201684L1 | Lenti ORF clone of Human SAC1 suppressor of actin mutations 1-like (yeast) (SACM1L), Myc-DDK-tagged |
CNY 8,952.00 |
|
| RC201684L2 | Lenti ORF clone of Human SAC1 suppressor of actin mutations 1-like (yeast) (SACM1L), mGFP tagged |
CNY 6,460.00 |
|
| RC201684L3 | Lenti ORF clone of Human SAC1 suppressor of actin mutations 1-like (yeast) (SACM1L), Myc-DDK-tagged |
CNY 6,460.00 |
|
| RC201684L4 | Lenti ORF clone of Human SAC1 suppressor of actin mutations 1-like (yeast) (SACM1L), mGFP tagged |
CNY 6,460.00 |
|
| RG201684 | SACM1L (tGFP-tagged) - Human SAC1 suppressor of actin mutations 1-like (yeast) (SACM1L) |
CNY 8,152.00 |
|
| SC115176 | SACM1L (untagged)-Human SAC1 suppressor of actin mutations 1-like (yeast) (SACM1L) |
CNY 6,568.00 |
