SNRP70 (SNRNP70) (NM_003089) Human Tagged ORF Clone

Name: SNRP70 (SNRNP70) (NM_003089) Human Tagged ORF Clone
SKU: RC201713
Price: 3656.00

CAT＃: RC201713

SNRNP70 (Myc-DDK-tagged)-Human small nuclear ribonucleoprotein 70kDa (U1) (SNRNP70)

ORF Plasmid: DDK tGFP

Datasheet

SDS

Plasmid Map

"NM_003089" in other vectors (6)

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CNY 3,656.00

CNY 3,990.00

货期*

现货

规格

Cited in 2 publications.

Product images

经常一起买 (5)

PS100001

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CC100003

DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)

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PRIMER

Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each

CNY 480.00

TA592569S

DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags

CNY 300.00

【特别福利】购一抗正装产品（≥50ul或≥50ug），免费领10ul抗体试用装

TA345725

Rabbit Polyclonal Anti-SNRP70 Antibody

CNY 5,250.00

Specifications

Product Data
Type	Human Tagged ORF Clone
Tag	Myc-DDK
Synonyms	RNPU1Z; RPU1; Snp1; SNRP70; U1-70K; U1AP; U1RNP; U170K
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RC201713 ORF sequence Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGACCCAGTTCCTGCCGCCCAACCTTCTGGCCCTCTTTGCCCCCCGTGACCCTATTCCATACCTGCCAC CCCTGGAGAAACTGCCACATGAAAAACACCACAATCAACCTTATTGTGGCATTGCGCCGTACATTCGAGA GTTTGAGGACCCTCGAGATGCCCCTCCTCCAACTCGTGCTGAAACCCGAGAGGAGCGCATGGAGAGGAAA AGACGGGAAAAGATTGAGCGGCGACAGCAAGAAGTGGAGACAGAGCTTAAAATGTGGGACCCTCACAATG ATCCCAATGCTCAGGGGGATGCCTTCAAGACTCTCTTCGTGGCGAGAGTGAATTATGACACAACAGAATC CAAGCTCCGGAGAGAGTTTGAGGTGTACGGACCTATCAAAAGAATACACATGGTCTACAGTAAGCGGTCA GGAAAGCCCCGTGGCTATGCCTTCATCGAGTACGAACACGAGCGAGACATGCACTCCGCTTACAAACACG CAGATGGCAAGAAGATTGATGGCAGGAGGGTCCTTGTGGACGTGGAGAGGGGCCGAACCGTGAAGGGCTG GAGGCCCCGGCGGCTAGGAGGAGGCCTCGGTGGTACCAGAAGAGGAGGGGCTGATGTGAACATCCGGCAT TCAGGCCGCGATGACACCTCCCGCTACGATGAGAGGCCCGGCCCCTCCCCGCTTCCGCACAGGGACCGGG ACCGGGACCGTGAGCGGGAGCGCAGAGAGCGGAGCCGGGAGCGAGACAAGGAGCGAGAACGGCGACGCTC CCGCTCCCGGGACCGGCGGAGGCGCTCACGGAGTCGCGACAAGGAGGAGCGGAGGCGCTCCAGGGAGCGG AGCAAGGACAAGGACCGGGACCGGAAGCGGCGAAGCAGCCGGAGTCGGGAGCGGGCCCGGCGGGAGCGGG AGCGCAAGGAGGAGCTGCGTGGTGGCGGTGGCGACATGGCGGAGCCCTCCGAGGCGGGTGACGCGCCCCC TGATGATGGGCCTCCAGGGGAGCTCGGGCCTGACGGCCCTGACGGTCCAGAGGAAAAGGGCCGGGATCGT GACCGGGAGCGACGGCGGAGCCACCGGAGCGAGCGCGAGCGGCGCCGGGACCGGGATCGTGACCGTGACC GTGACCGCGAGCACAAACGGGGGGAGCGGGGCAGTGAGCGGGGCAGGGATGAGGCCCGAGGTGGGGGCGG TGGCCAGGACAACGGGCTGGAGGGTCTGGGCAACGACAGCCGAGACATGTACATGGAGTCTGAGGGCGGC GACGGCTACCTGGCTCCGGAGAATGGGTATTTGATGGAGGCTGCGCCGGAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RC201713 protein sequence Red=Cloning site Green=Tags(s) MTQFLPPNLLALFAPRDPIPYLPPLEKLPHEKHHNQPYCGIAPYIREFEDPRDAPPPTRAETREERMERK RREKIERRQQEVETELKMWDPHNDPNAQGDAFKTLFVARVNYDTTESKLRREFEVYGPIKRIHMVYSKRS GKPRGYAFIEYEHERDMHSAYKHADGKKIDGRRVLVDVERGRTVKGWRPRRLGGGLGGTRRGGADVNIRH SGRDDTSRYDERPGPSPLPHRDRDRDRERERRERSRERDKERERRRSRSRDRRRRSRSRDKEERRRSRER SKDKDRDRKRRSSRSRERARRERERKEELRGGGGDMAEPSEAGDAPPDDGPPGELGPDGPDGPEEKGRDR DRERRRSHRSERERRRDRDRDRDRDREHKRGERGSERGRDEARGGGGGQDNGLEGLGNDSRDMYMESEGG DGYLAPENGYLMEAAPE TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms	CHROMATOGRAMS Sequencher program is needed, download here.
Restriction Sites	SgfI-MluI Cloning Scheme for this gene Plasmid Map
ACCN	NM_003089
ORF Size	1311 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_003089.3
RefSeq Size	2008 bp
RefSeq ORF	1314 bp
Locus ID	6625
UniProt ID	P08621
Protein Families	Druggable Genome
Protein Pathways	Spliceosome
MW	51.6 kDa
Gene Summary	Component of the spliceosomal U1 snRNP, which is essential for recognition of the pre-mRNA 5' splice-site and the subsequent assembly of the spliceosome (PubMed:19325628, PubMed:25555158). SNRNP70 binds to the loop I region of U1-snRNA (PubMed:2467746, PubMed:19325628, PubMed:25555158).[UniProtKB/Swiss-Prot Function]

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

Citations (2)

The use of this cDNA Clones has been cited in the following citations:
Integrated Approaches for Analyzing U1-70K Cleavage in Alzheimer’s Disease ,null, Journal of Proteome Research ,PubMed ID 24902715 [SNRP70]
Aggregation Properties of the Small Nuclear Ribonucleoprotein U1-70K in Alzheimer Disease ,Diner, I;Hales, CM;Rabenold, L;Bishof, I;Duong, DM;Yi, H;Laur, O;Gearing, M;Troncoso, J;Thambisetty, M;Lah, JJ;Levey, AI;Seyfried, NT;, J. Biol. Chem. ,PubMed ID 25355317 [SNRP70]

Documents

Product Manuals
TrueORFs: Open Reading Frame Clones - 10 ug in PrecisionShuttle Vector System pLenti-ORF destination vector Transfection - TurboFectin 8.0 Transfection Reagent Plasmid Preparation - PowerPrep Plasmid Purification Kits
FAQs
TrueORF Clones/PrecisionShuttle Organelle Marker Transfection Reagent
SDS
TrueORF SDS

Resources

cDNA 克隆相关资源
Video Tutorial: Clone Selection Guide Product Video: What is TrueORF GOLD and why it is the most popular type of clone Video Tutorial: How to make Lentivirus particles Video Tutorial: Is the Lentivirus Safe Product Brochure: cDNA clones $98 Clones. Download the List of 15,000 Protocol: Lentivirus packaging

Other Versions

SKU	Description	Size	Price
RC201713L1	Lenti ORF clone of Human small nuclear ribonucleoprotein 70kDa (U1) (SNRNP70), Myc-DDK-tagged	10 ug 产品选项验证失败所有选项都是必需的	CNY 6,056.00
RC201713L2	Lenti ORF clone of Human small nuclear ribonucleoprotein 70kDa (U1) (SNRNP70), mGFP tagged	10 ug 产品选项验证失败所有选项都是必需的	CNY 5,890.00
RC201713L3	Lenti ORF clone of Human small nuclear ribonucleoprotein 70kDa (U1) (SNRNP70), Myc-DDK-tagged	10 ug 产品选项验证失败所有选项都是必需的	CNY 5,890.00
RC201713L4	Lenti ORF clone of Human small nuclear ribonucleoprotein 70kDa (U1) (SNRNP70), mGFP tagged	10 ug 产品选项验证失败所有选项都是必需的	CNY 5,890.00
RG201713	SNRNP70 (tGFP-tagged) - Human small nuclear ribonucleoprotein 70kDa (U1) (SNRNP70)	10 ug 产品选项验证失败所有选项都是必需的	CNY 5,256.00
SC321910	SNRNP70 (untagged)-Human small nuclear ribonucleoprotein 70kDa (U1) (SNRNP70)	10 ug 产品选项验证失败所有选项都是必需的	CNY 3,656.00