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HEAB (CLP1) (NM_006831) Human Tagged ORF Clone
CAT＃: RC201772
CLP1 (Myc-DDK-tagged)-Human CLP1, cleavage and polyadenylation factor I subunit, homolog (S. cerevisiae) (CLP1), transcript variant 1
ORF Plasmid: tGFP
"NM_006831" in other vectors (6)
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CNY 3,656.00
CNY 3,990.00
Product images
CNY 300.00
CNY 1,999.00
CNY 3,340.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | hClp1; HEAB |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC201772 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGAGAAGAGGCTAATGATGACAAGAAGCCAACCACTAAATTTGAACTAGAGCGAGAAACAGAACTTC GCTTTGAGGTGGAGGCATCTCAGTCAGTTCAGTTGGAGTTGTTGACTGGCATGGCAGAGATCTTTGGCAC AGAGCTGACCCGAAACAAGAAATTCACCTTTGATGCTGGTGCCAAGGTGGCTGTTTTCACTTGGCATGGC TGTTCTGTGCAACTGAGCGGCCGCACTGAGGTGGCTTATGTCTCCAAGGACACTCCTATGTTGCTTTACC TCAACACTCACACAGCCTTGGAACAGATGCGGAGGCAAGCGGAAAAGGAAGAAGAGCGAGGTCCCCGAGT GATGGTAGTGGGCCCCACTGATGTGGGCAAGTCTACAGTGTGTCGCCTTCTGCTCAACTACGCAGTGCGT TTGGGCCGCCGTCCCACTTATGTGGAGCTGGATGTGGGCCAGGGTTCTGTGTCCATCCCTGGTACCATGG GGGCCCTCTACATCGAGCGGCCTGCAGATGTCGAAGAGGGTTTCTCTATCCAGGCCCCTCTGGTGTATCA TTTTGGTTCCACCACTCCTGGCACTAACATCAAGCTTTATAATAAGATTACATCTCGTTTAGCAGATGTG TTCAACCAAAGGTGTGAGGTGAACCGAAGGGCATCTGTGAGTGGCTGTGTCATTAACACCTGTGGCTGGG TCAAGGGCTCTGGTTACCAGGCTCTGGTGCATGCAGCCTCAGCTTTTGAGGTGGATGTCGTTGTTGTTCT GGATCAAGAACGACTGTACAATGAACTGAAACGGGACCTCCCCCACTTTGTACGCACTGTGCTGCTCCCT AAATCTGGGGGTGTGGTGGAGCGCTCCAAGGACTTCCGGCGGGAATGTAGGGATGAGCGTATCCGTGAGT ATTTTTATGGATTCCGAGGCTGTTTCTATCCCCATGCCTTCAATGTCAAATTTTCAGATGTGAAAATCTA CAAAGTTGGGGCACCCACCATCCCAGACTCCTGTTTACCTTTGGGCATGTCTCAAGAGGATAATCAGCTC AAGCTAGTACCTGTCACTCCTGGGCGAGATATGGTGCACCACCTACTGAGTGTTAGCACTGCCGAGGGTA CAGAGGAGAACCTGTCCGAGACAAGTGTAGCTGGCTTCATTGTGGTGACCAGTGTGGACCTGGAGCATCA GGTGTTTACTGTTCTGTCTCCAGCCCCTCGCCCACTGCCTAAGAACTTCCTTCTCATCATGGATATCCGG TTCATGGATCTGAAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC201772 protein sequence
Red=Cloning site Green=Tags(s) MGEEANDDKKPTTKFELERETELRFEVEASQSVQLELLTGMAEIFGTELTRNKKFTFDAGAKVAVFTWHG CSVQLSGRTEVAYVSKDTPMLLYLNTHTALEQMRRQAEKEEERGPRVMVVGPTDVGKSTVCRLLLNYAVR LGRRPTYVELDVGQGSVSIPGTMGALYIERPADVEEGFSIQAPLVYHFGSTTPGTNIKLYNKITSRLADV FNQRCEVNRRASVSGCVINTCGWVKGSGYQALVHAASAFEVDVVVVLDQERLYNELKRDLPHFVRTVLLP KSGGVVERSKDFRRECRDERIREYFYGFRGCFYPHAFNVKFSDVKIYKVGAPTIPDSCLPLGMSQEDNQL KLVPVTPGRDMVHHLLSVSTAEGTEENLSETSVAGFIVVTSVDLEHQVFTVLSPAPRPLPKNFLLIMDIR FMDLK TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_006831 |
| ORF Size | 1275 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_006831.3 |
| RefSeq Size | 1864 bp |
| RefSeq ORF | 1278 bp |
| Locus ID | 10978 |
| UniProt ID | Q92989 |
| MW | 47.6 kDa |
| Gene Summary | This gene encodes a member of the Clp1 family. The encoded protein is a multifunctional kinase which is a component of the tRNA splicing endonuclease complex and a component of the pre-mRNA cleavage complex II. This protein is implicated in tRNA, mRNA, and siRNA maturation. Mutations in this gene are associated with pontocerebellar hypoplasia type 10 (PCH10). Alternatively splice transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2014] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC201772L1 | Lenti ORF clone of Human CLP1, cleavage and polyadenylation factor I subunit, homolog (S. cerevisiae) (CLP1), transcript variant 1, Myc-DDK-tagged |
CNY 6,056.00 |
|
| RC201772L2 | Lenti ORF clone of Human CLP1, cleavage and polyadenylation factor I subunit, homolog (S. cerevisiae) (CLP1), transcript variant 1, mGFP tagged |
CNY 5,890.00 |
|
| RC201772L3 | Lenti ORF clone of Human CLP1, cleavage and polyadenylation factor I subunit, homolog (S. cerevisiae) (CLP1), transcript variant 1, Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC201772L4 | Lenti ORF clone of Human CLP1, cleavage and polyadenylation factor I subunit, homolog (S. cerevisiae) (CLP1), transcript variant 1, mGFP tagged |
CNY 5,890.00 |
|
| RG201772 | CLP1 (tGFP-tagged) - Human CLP1, cleavage and polyadenylation factor I subunit, homolog (S. cerevisiae) (CLP1), transcript variant 1 |
CNY 5,256.00 |
|
| SC115883 | CLP1 (untagged)-Human CLP1, cleavage and polyadenylation factor I subunit, homolog (S. cerevisiae) (CLP1), transcript variant 1 |
CNY 3,656.00 |
