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katanin p80 (KATNB1) (NM_005886) Human Tagged ORF Clone
CAT＃: RC201852
KATNB1 (Myc-DDK-tagged)-Human katanin p80 (WD repeat containing) subunit B 1 (KATNB1)
ORF Plasmid: tGFP
"NM_005886" in other vectors (4)
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CNY 4,880.00
Product images
CNY 300.00
CNY 1,999.00
CNY 2,700.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | KAT; LIS6 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC201852 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCCACCCCTGTGGTCACCAAGACAGCCTGGAAGTTGCAAGAGATCGTCGCGCATGCCAGCAACGTGT CCTCACTGGTGCTGGGCAAAGCCTCCGGGCGGCTGCTGGCTACAGGCGGGGATGACTGCCGCGTCAACCT GTGGTCCATCAACAAGCCCAACTGCATCATGAGCCTGACGGGCCACACATCCCCAGTGGAGAGCGTCCGC CTCAACACCCCCGAGGAGCTCATCGTGGCCGGCTCTCAGTCGGGCTCCATCCGTGTCTGGGACCTGGAAG CTGCCAAAATTCTTCGCACACTCATGGGACACAAAGCCAACATCTGCAGCCTGGATTTCCACCCGTACGG CGAGTTTGTAGCCTCTGGTTCCCAGGACACAAACATCAAGCTCTGGGACATCAGGAGGAAAGGCTGTGTC TTCCGATACAGGGGGCACAGCCAGGCCGTGCGGTGTCTCCGGTTCAGCCCCGATGGGAAGTGGTTGGCGT CGGCCGCAGATGACCACACCGTGAAGCTCTGGGATCTCACTGCCGGCAAGATGATGTCTGAGTTCCCTGG TCACACGGGGCCTGTCAACGTGGTCGAGTTTCACCCCAACGAGTACCTCCTGGCCTCCGGCAGCTCTGAC AGGACAATCCGCTTCTGGGACCTGGAGAAGTTCCAGGTGGTGAGCTGCATCGAAGGGGAGCCTGGGCCCG TCAGGAGCGTCCTCTTCAACCCAGATGGCTGCTGCCTGTACAGCGGCTGCCAGGACTCACTGCGTGTCTA CGGCTGGGAACCTGAGCGGTGCTTTGATGTGGTCCTCGTCAACTGGGGCAAGGTGGCCGACCTGGCCATC TGCAATGACCAGTTGATAGGTGTGGCCTTCTCCCAGAGCAACGTCTCCTCCTACGTGGTGGATCTGACGC GTGTCACCAGGACTGGCACGGTGGCCCGGGACCCTGTGCAGGACCACCGGCCCCTGGCACAGCCACTGCC CAACCCCAGCGCCCCCCTCCGGCGCATCTATGAGCGGCCCAGCACAACCTGCAGCAAGCCTCAGAGGGTG AAGCAGAACTCAGAGAGCGAGCGCCGCAGCCCCAGCAGCGAGGATGACCGGGACGAGCGCGAGTCCCGCG CGGAGATCCAGAACGCCGAGGACTACAACGAGATCTTCCAGCCCAAGAACAGCATCAGTCGGACGCCACC CCGGAGAAGTGAGCCCTTCCCTGCACCCCCAGAGGACGACGCAGCCACAGCAAAGGAGGCAGCAAAGCCC AGCCCTGCCATGGATGTGCAGTTCCCGGTGCCAAATCTGGAGGTCCTGCCCCGGCCCCCAGTGGTTGCTT CCACACCTGCACCCAAGGCTGAGCCTGCCATCATCCCTGCCACCCGGAACGAGCCCATCGGGCTGAAGGC CTCCGACTTCCTGCCCGCCGTGAAGATCCCCCAGCAGGCCGAGCTGGTGGACGAGGATGCCATGTCACAG ATCCGCAAAGGCCACGACACCATGTGTGTGGTGCTCACCAGCCGCCACAAGAACCTGGACACTGTGCGGG CTGTGTGGACCATGGGCGACATCAAGACGTCGGTGGACTCCGCTGTGGCCATCAACGACCTGTCGGTGGT GGTGGACCTCCTGAACATCGTCAACCAGAAAGCCTCCCTGTGGAAGCTGGACCTGTGCACCACCGTCCTG CCACAGATTGAGAAGCTTCTGCAGAGCAAGTATGAGAGCTACGTCCAGACGGGCTGCACCTCCCTGAAGC TGATCCTGCAGCGGTTTCTGCCCCTCATCACAGACATGCTGGCGGCCCCACCCTCTGTGGGTGTGGATAT CAGCAGGGAGGAGAGGCTGCATAAGTGCCGGCTCTGCTACAAGCAGCTTAAGAGCATCAGCGGCCTGGTC AAGAGCAAGTCAGGCCTGAGCGGCCGCCATGGCAGTACCTTCCGCGAGCTGCACCTGCTCATGGCCAGTC TGGAC AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC TGGATTACAAGGATGACGACGATAAGGTTTAA >RC201852 protein sequence
Red=Cloning site Green=Tags(s) MATPVVTKTAWKLQEIVAHASNVSSLVLGKASGRLLATGGDDCRVNLWSINKPNCIMSLTGHTSPVESVR LNTPEELIVAGSQSGSIRVWDLEAAKILRTLMGHKANICSLDFHPYGEFVASGSQDTNIKLWDIRRKGCV FRYRGHSQAVRCLRFSPDGKWLASAADDHTVKLWDLTAGKMMSEFPGHTGPVNVVEFHPNEYLLASGSSD RTIRFWDLEKFQVVSCIEGEPGPVRSVLFNPDGCCLYSGCQDSLRVYGWEPERCFDVVLVNWGKVADLAI CNDQLIGVAFSQSNVSSYVVDLTRVTRTGTVARDPVQDHRPLAQPLPNPSAPLRRIYERPSTTCSKPQRV KQNSESERRSPSSEDDRDERESRAEIQNAEDYNEIFQPKNSISRTPPRRSEPFPAPPEDDAATAKEAAKP SPAMDVQFPVPNLEVLPRPPVVASTPAPKAEPAIIPATRNEPIGLKASDFLPAVKIPQQAELVDEDAMSQ IRKGHDTMCVVLTSRHKNLDTVRAVWTMGDIKTSVDSAVAINDLSVVVDLLNIVNQKASLWKLDLCTTVL PQIEKLLQSKYESYVQTGCTSLKLILQRFLPLITDMLAAPPSVGVDISREERLHKCRLCYKQLKSISGLV KSKSGLSGRHGSTFRELHLLMASLD SGPTRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-RsrII
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_005886 |
| ORF Size | 1965 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_005886.3 |
| RefSeq Size | 2661 bp |
| RefSeq ORF | 1968 bp |
| Locus ID | 10300 |
| UniProt ID | Q9BVA0 |
| Domains | WD40 |
| MW | 72.3 kDa |
| Gene Summary | Microtubules, polymers of alpha and beta tubulin subunits, form the mitotic spindle of a dividing cell and help to organize membranous organelles during interphase. Katanin is a heterodimer that consists of a 60 kDa ATPase (p60 subunit A 1) and an 80 kDa accessory protein (p80 subunit B 1). The p60 subunit acts to sever and disassemble microtubules, while the p80 subunit targets the enzyme to the centrosome. Katanin is a member of the AAA family of ATPases. [provided by RefSeq, Jul 2008] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC201852L3 | Lenti ORF clone of Human katanin p80 (WD repeat containing) subunit B 1 (KATNB1), Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC201852L4 | Lenti ORF clone of Human katanin p80 (WD repeat containing) subunit B 1 (KATNB1), mGFP tagged |
CNY 5,890.00 |
|
| RG201852 | KATNB1 (tGFP-tagged) - Human katanin p80 (WD repeat containing) subunit B 1 (KATNB1) |
CNY 6,480.00 |
|
| SC319053 | KATNB1 (untagged)-Human katanin p80 (WD repeat containing) subunit B 1 (KATNB1) |
CNY 4,888.00 |
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