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TRIM31 (NM_007028) Human Tagged ORF Clone
CAT＃: RC201930
TRIM31 (Myc-DDK-tagged)-Human tripartite motif containing 31 (TRIM31)
ORF Plasmid: tGFP
"NM_007028" in other vectors (6)
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CNY 3,656.00
CNY 3,990.00
| Cited in 2 publications. |
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | C6orf13; HCG1; HCGI; RNF |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC201930 representing NM_007028
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCCAGTGGGCAGTTTGTGAACAAACTGCAAGAGGAAGTGATCTGCCCCATCTGCCTGGACATTCTGC AGAAACCTGTCACCATCGACTGTGGGCACAATTTCTGCCTCAAATGCATCACTCAGATTGGGGAAACATC ATGTGGATTTTTCAAATGTCCCCTCTGCAAAACTTCCGTAAGGAAGAACGCAATCAGGTTCAACTCGCTG TTGCGGAATCTGGTGGAGAAAATCCAAGCTCTACAAGCCTCTGAGGTGCAGTCCAAAAGGAAAGAGGCTA CATGCCCGAGGCACCAGGAGATGTTCCACTATTTCTGCGAGGATGATGGGAAGTTCCTCTGTTTTGTGTG TCGTGAATCCAAGGACCACAAATCCCATAATGTCAGCTTGATCGAAGAAGCTGCCCAGAATTATCAGGGG CAGATTCAAGAGCAGATCCAAGTCTTGCAGCAAAAGGAGAAGGAGACAGTACAAGTGAAGGCACAAGGTG TACACAGGGTCGATGTCTTCACGGACCAGGTAGAACATGAGAAGCAAAGGATCCTCACAGAATTTGAACT CCTGCATCAAGTCCTAGAGGAGGAGAAGAATTTCCTGCTATCACGGATTTACTGGCTGGGTCATGAGGGA ACGGAAGCGGGGAAACACTATGTTGCCTCCACTGAGCCACAGTTGAACGATCTCAAGAAGCTCGTTGATT CCCTGAAGACCAAGCAGAACATGCCACCCAGGCAGCTGCTGGAGGATATCAAAGTCGTCTTGTGCAGAAG TGAAGAGTTTCAGTTTCTCAACCCAACCCCTGTTCCTCTGGAACTGGAGAAAAAACTCAGTGAAGCAAAA TCAAGACACGACTCCATCACAGGGAGCCTGAAAAAATTCAAAGACCAACTCCAGGCTGATAGGAAAAAAG ATGAAAACAGATTCTTCAAAAGCATGAATAAAAATGACATGAAGAGCTGGGGCTTGTTACAGAAAAATAA TCATAAAATGAACAAAACCTCAGAGCCCGGGTCATCTTCTGCAGGCGGCAGAACTACATCGGGGCCACCA AATCACCACTCTTCAGCCCCATCCCACTCCCTGTTTCGGGCCTCGTCTGCTGGGAAAGTCACTTTTCCAG TATGTCTCCTGGCCTCTTATGATGAGATTTCTGGTCAAGGAGCGAGCTCTCAGGATACGAAGACATTTGA CGTTGCGCTGTCCGAGGAGCTCCATGCGGCACTGAGTGAGTGGCTGACAGCGATCCGGGCTTGGTTTTGT AAGGTTCCTTCAAGC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC201930 representing NM_007028
Red=Cloning site Green=Tags(s) MASGQFVNKLQEEVICPICLDILQKPVTIDCGHNFCLKCITQIGETSCGFFKCPLCKTSVRKNAIRFNSL LRNLVEKIQALQASEVQSKRKEATCPRHQEMFHYFCEDDGKFLCFVCRESKDHKSHNVSLIEEAAQNYQG QIQEQIQVLQQKEKETVQVKAQGVHRVDVFTDQVEHEKQRILTEFELLHQVLEEEKNFLLSRIYWLGHEG TEAGKHYVASTEPQLNDLKKLVDSLKTKQNMPPRQLLEDIKVVLCRSEEFQFLNPTPVPLELEKKLSEAK SRHDSITGSLKKFKDQLQADRKKDENRFFKSMNKNDMKSWGLLQKNNHKMNKTSEPGSSSAGGRTTSGPP NHHSSAPSHSLFRASSAGKVTFPVCLLASYDEISGQGASSQDTKTFDVALSEELHAALSEWLTAIRAWFC KVPSS TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_007028 |
| ORF Size | 1275 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_007028.5 |
| RefSeq Size | 2049 bp |
| RefSeq ORF | 1278 bp |
| Locus ID | 11074 |
| UniProt ID | Q9BZY9 |
| MW | 48.1 kDa |
| Gene Summary | This gene encodes a protein that functions as an E3 ubiquitin-protein ligase. This gene shows altered expression in certain tumors and may be a negative regulator of cell growth. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2016] |
Citations (2)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
TRIM31 promotes Atg5/Atg7-independent autophagy in intestinal cells
,Ra, EA;Lee, TA;Won Kim, S;Park, A;Choi, HJ;Jang, I;Kang, S;Hee Cheon, J;Cho, JW;Eun Lee, J;Lee, S;Park, B;,
Nat Commun
,PubMed ID 27216961
[TRIM31]
|
|
The Melanoma-associated Antigen 1 (MAGEA1) Protein Stimulates the E3 Ubiquitin-ligase Activity of TRIM31 within a TRIM31-MAGEA1-NSE4 Complex
,Kozakova, L;Vondrova, L;Stejskal, K;Charalabous, P;Kolesar, P;Lehmann, AR;Uldrijan, S;Sanderson, CM;Zdrahal, Z;Palecek, JJ;,
Cell Cycle
,PubMed ID 25590999
[TRIM31]
|
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC201930L1 | Lenti ORF clone of Human tripartite motif containing 31 (TRIM31), Myc-DDK-tagged |
CNY 6,056.00 |
|
| RC201930L2 | Lenti ORF clone of Human tripartite motif containing 31 (TRIM31), mGFP tagged |
CNY 5,890.00 |
|
| RC201930L3 | Lenti ORF clone of Human tripartite motif containing 31 (TRIM31), Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC201930L4 | Lenti ORF clone of Human tripartite motif containing 31 (TRIM31), mGFP tagged |
CNY 5,890.00 |
|
| RG201930 | TRIM31 (tGFP-tagged) - Human tripartite motif containing 31 (TRIM31) |
CNY 5,256.00 |
|
| SC126105 | TRIM31 (untagged)-Human tripartite motif containing 31 (TRIM31) |
CNY 3,656.00 |
