NCKAP1 (NM_013436) Human Tagged ORF Clone
CAT#: RC201960
NCKAP1 (Myc-DDK-tagged)-Human NCK-associated protein 1 (NCKAP1), transcript variant 1
ORF Plasmid: tGFP
"NM_013436" in other vectors (6)
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CNY 7,856.00
Cited in 2 publications. |
CNY 300.00
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | HEM2; NAP1; NAP125; p125Nap1 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RC201960 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCGCGCTCAGTGCTGCAGCCCAGTCAGCAGAAGCTGGCGGAGAAGCTCACCATCCTCAACGACCGGG GCGTCGGCATGCTCACCCGCCTCTACAACATCAAGAAGGCATGTGGAGACCCCAAGGCAAAACCATCCTA TCTTATCGACAAAAACCTGGAATCTGCTGTGAAATTCATAGTCAGAAAATTCCCTGCTGTAGAAACCCGC AACAACAATCAACAGCTTGCACAACTACAGAAAGAAAAATCAGAGATTCTGAAAAATCTGGCATTATATT ACTTCACATTTGTAGATGTTATGGAATTTAAGGACCATGTTTGTGAATTGCTGAATACTATTGACGTTTG CCAAGTCTTCTTTGATATTACTGTAAACTTTGATTTAACAAAGAACTACTTAGATTTAATTATAACCTAT ACAACACTAATGATACTGCTGTCTCGAATTGAAGAAAGGAAGGCAATCATTGGATTATACAACTATGCCC ATGAAATGACTCATGGAGCAAGTGACAGAGAATACCCACGCCTTGGCCAGATGATTGTGGATTATGAAAA CCCTTTAAAGAAGATGATGGAAGAATTTGTACCCCATAGCAAGTCTCTTTCAGATGCACTAATTTCTCTT CAAATGGTATATCCTCGAAGGAATCTTTCAGCTGACCAGTGGAGAAATGCCCAGTTATTGAGCCTCATCA GTGCACCTAGTACAATGCTTAATCCAGCACAGTCCGACACTATGCCTTGTGAATACCTCTCTTTGGATGC AATGGAAAAGTGGATTATCTTTGGCTTTATTTTGTGCCATGGGATCCTAAATACTGACGCTACAGCACTG AACCTTTGGAAACTAGCTCTTCAAAGTAGCTCTTGCCTCTCTCTCTTTCGGGATGAAGTTTTCCACATTC ACAAAGCTGCAGAAGACTTATTTGTAAACATACGAGGCTATAATAAACGTATTAATGACATAAGAGAATG CAAGGAGGCAGCCGTGTCACATGCTGGTTCAATGCACAGAGAAAGACGCAAGTTTTTAAGATCTGCACTG AAGGAATTGGCTACTGTCCTCTCTGATCAACCTGGATTGCTAGGTCCCAAGGCACTTTTTGTTTTTATGG CATTATCCTTTGCCCGTGATGAAATCATCTGGCTACTTCGTCATGCAGATAACATGCCAAAGAAGAGTGC AGACGACTTTATAGATAAGCACATTGCTGAATTAATATTTTACATGGAAGAACTTAGAGCACATGTGAGG AAATACGGACCTGTAATGCAGAGGTATTACGTGCAGTACCTTTCTGGCTTTGATGCTGTTGTCCTCAATG AACTCGTGCAGAATCTTTCTGTTTGCCCTGAAGATGAATCAATCATCATGTCCTCTTTTGTTAACACTAT GACTTCCCTAAGTGTAAAACAAGTTGAAGATGGGGAAGTATTTGATTTCAGAGGAATGAGATTAGATTGG TTTAGGTTACAGGCATATACTAGTGTCTCAAAGGCTTCACTTGGCCTTGCAGATCACAGAGAACTTGGAA AGATGATGAATACAATAATTTTTCATACAAAAATGGTAGATTCCTTGGTGGAAATGTTGGTGGAAACATC AGATCTCTCCATATTTTGTTTTTATAGTCGTGCTTTTGAGAAGATGTTTCAACAGTGTTTGGAGTTACCC TCTCAATCAAGATACTCAATTGCATTTCCACTACTTTGCACTCATTTTATGAGTTGCACGCATGAACTAT GTCCAGAAGAGCGACATCATATTGGAGATCGCAGTCTTTCCTTATGTAATATGTTCCTAGATGAAATGGC CAAACAAGCTCGAAATCTCATCACTGATATTTGCACAGAACAGTGTACCCTTAGTGACCAGTTGCTACCC AAGCATTGTGCCAAAACTATCAGTCAAGCAGTGAATAAGAAATCAAAAAAGCAGACTGGTAAGAAAGGGG AACCTGAAAGGGAGAAACCAGGTGTTGAGAGCATGAGGAAAAACAGGCTGGTTGTGACCAACCTTGATAA ATTGCACACTGCACTTTCTGAGTTATGCTTCTCTATAAATTATGTACCAAACATGGTGGTATGGGAACAT ACCTTTACCCCACGAGAATATTTGACTTCTCATCTGGAAATACGCTTTACCAAGTCAATTGTTGGGATGA CTATGTATAATCAAGCCACACAGGAAATTGCAAAACCTTCAGAACTTCTAACAAGTGTAAGAGCATACAT GACCGTACTCCAGTCAATAGAAAACTATGTGCAGATTGATATTACAAGAGTATTTAATAATGTGCTTCTT CAACAAACACAACATTTAGACAGTCATGGAGAGCCAACCATTACAAGTCTATACACAAATTGGTATTTGG AAACTTTGTTACGACAAGTCAGCAATGGCCATATAGCATATTTTCCTGCAATGAAAGCGTTTGTGAACTT ACCTACAGAAAATGAATTAACATTCAATGCAGAGGAATATTCTGACATATCAGAAATGAGGTCATTATCA GAACTACTAGGCCCATATGGTATGAAGTTTCTAAGTGAAAGCCTTATGTGGCATATTTCATCACAAGTTG CTGAACTTAAGAAACTTGTGGTGGAGAATGTTGATGTGTTAACACAAATGAGGACCAGCTTTGACAAACC AGACCAGATGGCTGCACTGTTTAAAAGATTATCATCTGTTGACAGTGTCTTGAAGAGGATGACAATAATT GGTGTAATTTTATCCTTCCGATCATTGGCACAAGAAGCACTTAGAGATGTCTTATCCTACCACATTCCTT TTCTTGTAAGTTCAATTGAAGATTTTAAGGATCACATTCCAAGGGAAACTGATATGAAGGTTGCAATGAA TGTGTATGAGTTATCATCAGCTGCCGGATTACCTTGTGAGATTGATCCTGCATTGGTCGTAGCTCTTTCT TCACAAAAATCGGAAAACATTAGTCCAGAAGAAGAGTATAAAATTGCCTGCCTTCTCATGGTGTTTGTGG CAGTTTCTTTGCCAACACTGGCCAGTAATGTGATGTCTCAGTACAGCCCTGCTATAGAAGGGCATTGCAA CAACATACATTGCTTGGCCAAAGCCATCAACCAGATTGCTGCAGCTTTGTTTACAATTCACAAAGGAAGC ATTGAAGACCGTCTTAAAGAATTTCTGGCGCTTGCATCCTCCAGTCTACTGAAAATTGGCCAGGAGACAG ATAAAACTACAACAAGAAATAGAGAATCTGTTTATTTACTGCTAGATATGATTGTACAAGAATCTCCATT CCTTACAATGGATCTTTTGGAATCTTGTTTTCCTTATGTCTTGCTGAGAAATGCATACCATGCTGTCTAC AAACAAAGTGTTACATCTTCTGCA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC201960 protein sequence
Red=Cloning site Green=Tags(s) MSRSVLQPSQQKLAEKLTILNDRGVGMLTRLYNIKKACGDPKAKPSYLIDKNLESAVKFIVRKFPAVETR NNNQQLAQLQKEKSEILKNLALYYFTFVDVMEFKDHVCELLNTIDVCQVFFDITVNFDLTKNYLDLIITY TTLMILLSRIEERKAIIGLYNYAHEMTHGASDREYPRLGQMIVDYENPLKKMMEEFVPHSKSLSDALISL QMVYPRRNLSADQWRNAQLLSLISAPSTMLNPAQSDTMPCEYLSLDAMEKWIIFGFILCHGILNTDATAL NLWKLALQSSSCLSLFRDEVFHIHKAAEDLFVNIRGYNKRINDIRECKEAAVSHAGSMHRERRKFLRSAL KELATVLSDQPGLLGPKALFVFMALSFARDEIIWLLRHADNMPKKSADDFIDKHIAELIFYMEELRAHVR KYGPVMQRYYVQYLSGFDAVVLNELVQNLSVCPEDESIIMSSFVNTMTSLSVKQVEDGEVFDFRGMRLDW FRLQAYTSVSKASLGLADHRELGKMMNTIIFHTKMVDSLVEMLVETSDLSIFCFYSRAFEKMFQQCLELP SQSRYSIAFPLLCTHFMSCTHELCPEERHHIGDRSLSLCNMFLDEMAKQARNLITDICTEQCTLSDQLLP KHCAKTISQAVNKKSKKQTGKKGEPEREKPGVESMRKNRLVVTNLDKLHTALSELCFSINYVPNMVVWEH TFTPREYLTSHLEIRFTKSIVGMTMYNQATQEIAKPSELLTSVRAYMTVLQSIENYVQIDITRVFNNVLL QQTQHLDSHGEPTITSLYTNWYLETLLRQVSNGHIAYFPAMKAFVNLPTENELTFNAEEYSDISEMRSLS ELLGPYGMKFLSESLMWHISSQVAELKKLVVENVDVLTQMRTSFDKPDQMAALFKRLSSVDSVLKRMTII GVILSFRSLAQEALRDVLSYHIPFLVSSIEDFKDHIPRETDMKVAMNVYELSSAAGLPCEIDPALVVALS SQKSENISPEEEYKIACLLMVFVAVSLPTLASNVMSQYSPAIEGHCNNIHCLAKAINQIAAALFTIHKGS IEDRLKEFLALASSSLLKIGQETDKTTTRNRESVYLLLDMIVQESPFLTMDLLESCFPYVLLRNAYHAVY KQSVTSSA TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
![]() Sequencher program is needed, download here. |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_013436 |
ORF Size | 3384 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_013436.5 |
RefSeq Size | 4997 bp |
RefSeq ORF | 3387 bp |
Locus ID | 10787 |
UniProt ID | Q9Y2A7 |
Protein Families | Druggable Genome |
Protein Pathways | Regulation of actin cytoskeleton |
MW | 128.8 kDa |
Gene Summary | Part of the WAVE complex that regulates lamellipodia formation. The WAVE complex regulates actin filament reorganization via its interaction with the Arp2/3 complex. Actin remodeling activity is regulated by RAC1. As component of the WAVE1 complex, required for BDNF-NTRK2 endocytic trafficking and signaling from early endosomes.[UniProtKB/Swiss-Prot Function] |
Citations (2)
The use of this cDNA Clones has been cited in the following citations: |
---|
Role of NCKAP1 in the Defective Phagocytic Function of Microglia-Like Cells Derived from Rapidly Progressing Sporadic ALS
,null,
Molecular Neurobiology
,PubMed ID 37154887
[NCKAP1]
|
The WASF3-NCKAP1-CYFIP1 Complex Is Essential for Breast Cancer Metastasis
,Teng, Y;Qin, H;Bahassan, A;Bendzunas, NG;Kennedy, EJ;Cowell, JK;,
Cancer Res.
,PubMed ID 27432794
[NCKAP1]
|
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC201960L1 | Lenti ORF clone of Human NCK-associated protein 1 (NCKAP1), transcript variant 1, Myc-DDK-tagged |
CNY 11,590.00 |
|
RC201960L2 | Lenti ORF clone of Human NCK-associated protein 1 (NCKAP1), transcript variant 1, mGFP tagged |
CNY 10,256.00 |
|
RC201960L3 | Lenti ORF clone of Human NCK-associated protein 1 (NCKAP1), transcript variant 1, Myc-DDK-tagged |
CNY 11,590.00 |
|
RC201960L4 | Lenti ORF clone of Human NCK-associated protein 1 (NCKAP1), transcript variant 1, mGFP tagged |
CNY 10,256.00 |
|
RG201960 | NCKAP1 (tGFP-tagged) - Human NCK-associated protein 1 (NCKAP1), transcript variant 1 |
CNY 9,456.00 |
|
SC115236 | NCKAP1 (untagged)-Human NCK-associated protein 1 (NCKAP1), transcript variant 1 |
CNY 7,864.00 |