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PMM1 (NM_002676) Human Tagged ORF Clone
CAT＃: RC202004
PMM1 (Myc-DDK-tagged)-Human phosphomannomutase 1 (PMM1)
ORF Plasmid: tGFP
"NM_002676" in other vectors (5)
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CNY 2,400.00
CNY 3,705.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | PMM 1; PMMH-22; Sec53 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC202004 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCAGTCACCGCCCAGGCAGCCCGCAGGAAGGAGCGCGTCCTCTGCCTGTTTGACGTGGACGGGACCC TCACGCCGGCTCGCCAGAAAATTGACCCTGAGGTGGCCGCCTTCCTGCAGAAGCTACGAAGTAGAGTGCA GATCGGTGTGGTGGGCGGCTCTGACTACTGTAAGATCGCTGAGCAGCTGGGTGACGGGGATGAAGTCATT GAGAAGTTTGATTATGTGTTTGCCGAGAACGGGACGGTGCAGTATAAGCACGGACGACTGCTCTCCAAGC AGACCATCCAGAACCACCTGGGGGAGGAGCTGCTGCAGGACTTGATCAACTTCTGCCTCAGCTACATGGC CCTGCTCAGGCTGCCCAAGAAGCGTGGAACCTTCATCGAGTTCCGGAATGGCATGCTGAACATCTCGCCC ATCGGCCGGAGCTGCACCCTGGAGGAGAGGATCGAGTTCTCCGAACTGGACAAGAAAGAGAAGATCCGGG AGAAGTTCGTGGAAGCCCTGAAAACAGAGTTTGCTGGCAAAGGGCTGAGGTTCTCTCGAGGAGGCATGAT CAGCTTTGACGTCTTCCCCGAGGGCTGGGACAAGCGCTACTGCCTGGATAGCCTGGACCAGGACAGCTTC GACACCATCCACTTCTTTGGGAACGAGACTAGCCCTGGTGGGAACGACTTTGAGATCTTTGCCGACCCCC GGACTGTTGGCCACAGCGTGGTGTCTCCTCAGGACACGGTGCAGCGATGCCGGGAGATTTTCTTCCCAGA GACAGCTCATGAGGCG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC202004 protein sequence
Red=Cloning site Green=Tags(s) MAVTAQAARRKERVLCLFDVDGTLTPARQKIDPEVAAFLQKLRSRVQIGVVGGSDYCKIAEQLGDGDEVI EKFDYVFAENGTVQYKHGRLLSKQTIQNHLGEELLQDLINFCLSYMALLRLPKKRGTFIEFRNGMLNISP IGRSCTLEERIEFSELDKKEKIREKFVEALKTEFAGKGLRFSRGGMISFDVFPEGWDKRYCLDSLDQDSF DTIHFFGNETSPGGNDFEIFADPRTVGHSVVSPQDTVQRCREIFFPETAHEA TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_002676 |
| ORF Size | 786 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_002676.3 |
| RefSeq Size | 1295 bp |
| RefSeq ORF | 789 bp |
| Locus ID | 5372 |
| UniProt ID | Q92871 |
| Domains | PMM |
| Protein Pathways | Amino sugar and nucleotide sugar metabolism, Fructose and mannose metabolism, Metabolic pathways |
| MW | 29.7 kDa |
| Gene Summary | Phosphomannomutase catalyzes the conversion between D-mannose 6-phosphate and D-mannose 1-phosphate which is a substrate for GDP-mannose synthesis. GDP-mannose is used for synthesis of dolichol-phosphate-mannose, which is essential for N-linked glycosylation and thus the secretion of several glycoproteins as well as for the synthesis of glycosyl-phosphatidyl-inositol (GPI) anchored proteins. [provided by RefSeq, Jul 2008] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC202004L3 | Lenti ORF clone of Human phosphomannomutase 1 (PMM1), Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC202004L4 | Lenti ORF clone of Human phosphomannomutase 1 (PMM1), mGFP tagged |
CNY 5,890.00 |
|
| RG202004 | PMM1 (tGFP-tagged) - Human phosphomannomutase 1 (PMM1) |
CNY 4,000.00 |
|
| SC118505 | PMM1 (untagged)-Human phosphomannomutase 1 (PMM1) |
CNY 2,400.00 |
|
| SC322342 | PMM1 (untagged)-Human phosphomannomutase 1 (PMM1) |
CNY 2,400.00 |
