UBE2E1 (NM_003341) Human Tagged ORF Clone
CAT#: RC202106
- TrueORF®
UBE2E1 (Myc-DDK-tagged)-Human ubiquitin-conjugating enzyme E2E 1 (UBE2E1), transcript variant 1
ORF Plasmid: tGFP
"NM_003341" in other vectors (4)
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CNY 2,640.00
CNY 3,990.00
Cited in 1 publication. |
CNY 300.00
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | UBCH6 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_003341 |
ORF Size | 579 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_003341.5 |
RefSeq Size | 1845 bp |
RefSeq ORF | 582 bp |
Locus ID | 7324 |
UniProt ID | P51965 |
Domains | UBCc |
Protein Pathways | Ubiquitin mediated proteolysis |
MW | 21.4 kDa |
Gene Summary | The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. Three alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, Jan 2011] |
Citations (1)
The use of this cDNA Clones has been cited in the following citations: |
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Cbl interacts with multiple E2s in vitro and in cells
,Liyasova, MS;Ma, K;Voeller, D;Ryan, PE;Chen, J;Klevit, RE;Lipkowitz, S;,
PLoS ONE
,PubMed ID 31120930
[UBE2E1]
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Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC202106L3 | Lenti-ORF clone of UBE2E1 (Myc-DDK-tagged)-Human ubiquitin-conjugating enzyme E2E 1 (UBE2E1), transcript variant 1 |
CNY 5,890.00 |
|
RC202106L4 | Lenti-ORF clone of UBE2E1 (mGFP-tagged)-Human ubiquitin-conjugating enzyme E2E 1 (UBE2E1), transcript variant 1 |
CNY 5,890.00 |
|
RG202106 | UBE2E1 (tGFP-tagged) - Human ubiquitin-conjugating enzyme E2E 1 (UBE2E1), transcript variant 1 |
CNY 4,370.00 |
|
SC311117 | UBE2E1 (untagged)-Human ubiquitin-conjugating enzyme E2E 1 (UBE2E1), transcript variant 1 |
CNY 3,990.00 |