ACY3 (NM_080658) Human Tagged ORF Clone

CAT#: RC202287

ACY3 (Myc-DDK-tagged)-Human aspartoacylase (aminocyclase) 3 (ACY3)

ORF Plasmid: DDK tGFP



  "NM_080658" in other vectors (5)

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CNY 2,400.00

CNY 3,990.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (5)
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

CNY 5,420.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00


DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 30 ul

CNY 300.00


ACY3 mouse monoclonal antibody, clone OTI2D5 (formerly 2D5)
    • 100 ul

CNY 1,999.00
CNY 2,700.00

Specifications

Product Data
Type Human Tagged ORF Clone
Tag Myc-DDK
Synonyms ACY-3; HCBP1
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RC202287 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTGCTCACTGCCTGTGCCCCGGGAGCCCCTGCGTCGCGTGGCTGTGACTGGGGGCACGCATGGCAACG
AGATGTCGGGCGTCTACCTGGCCCGGCACTGGCTGCATGCCCCCGCAGAGCTGCAGAGAGCCAGCTTCTC
CGCTGTGCCTGTGCTGGCCAACCCGGCAGCCACATCCGGCTGCCGCCGCTACGTGGACCATGACCTCAAC
CGCACCTTCACCAGCAGCTTCCTCAATTCCAGGCCCACCCCGGACGACCCATATGAGGTGACAAGAGCCC
GAGAGCTGAACCAGCTGCTGGGGCCCAAGGCCTCGGGCCAGGCCTTTGACTTTGTCCTTGACCTGCACAA
CACCACGGCCAACATGGGCACCTGCTTAATCGCGAAGTCCTCCCACGAAGTCTTTGCCATGCACCTGTGC
CGCCATCTGCAGCTGCAGTACCCCGAGCTGTCCTGCCAGGTCTTCCTGTACCAGCGGTCTGGGGAGGAGA
GCTACAACCTGGACTCTGTGGCCAAAAATGGACTGGGTCTGGAGCTGGGCCCCCAGCCACAGGGTGTGCT
GCGGGCTGACATTTTCTCAAGGATGAGGACCCTGGTGGCCACAGTTCTGGACTTCATCGAACTCTTCAAC
CAGGGTACGGCCTTTCCTGCCTTTGAGATGGAAGCCTATAGACCCGTGGGCGTCGTGGACTTCCCCCGCA
CCGAGGCCGGGCACCTGGCAGGCACTGTGCATCCTCAGCTGCAGGACCGAGACTTCCAGCCACTGCAGCC
TGGTGCTCCCATCTTCCAGATGTTCAGTGGGGAGGACCTGCTCTATGAGGGAGAGTCCACGGTGTACCCC
GTGTTCATTAACGAGGCTGCCTACTATGAGAAGGGCGTTGCCTTTGTCCAGACTGAGAAGTTCACATTCA
CCGTGCCTGCCATGCCCGCGCTGACCCCTGCCCCGAGCCCAGCTTCC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RC202287 protein sequence
Red=Cloning site Green=Tags(s)

MCSLPVPREPLRRVAVTGGTHGNEMSGVYLARHWLHAPAELQRASFSAVPVLANPAATSGCRRYVDHDLN
RTFTSSFLNSRPTPDDPYEVTRARELNQLLGPKASGQAFDFVLDLHNTTANMGTCLIAKSSHEVFAMHLC
RHLQLQYPELSCQVFLYQRSGEESYNLDSVAKNGLGLELGPQPQGVLRADIFSRMRTLVATVLDFIELFN
QGTAFPAFEMEAYRPVGVVDFPRTEAGHLAGTVHPQLQDRDFQPLQPGAPIFQMFSGEDLLYEGESTVYP
VFINEAAYYEKGVAFVQTEKFTFTVPAMPALTPAPSPAS

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_080658
ORF Size 957 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_080658.2
RefSeq Size 1317 bp
RefSeq ORF 960 bp
Locus ID 91703
UniProt ID Q96HD9
Domains Aste_AspA
Protein Pathways Alanine, aspartate and glutamate metabolism, Histidine metabolism
MW 35.2 kDa
Gene Summary Plays an important role in deacetylating mercapturic acids in kidney proximal tubules. Also acts on N-acetyl-aromatic amino acids (By similarity).[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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